Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 289,091 A→G L24L (CTT→CTC)  xynR ← DNA‑binding transcriptional repressor XynR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913289,0910AG98.3% 225.7 / NA 59L24L (CTT→CTC) xynRDNA‑binding transcriptional repressor XynR
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (31/27);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACAC  >  NC_000913/288797‑289369
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                      
ggcggcCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTc                                                                                                                                                                                                                                                                                   <  2:307336/300‑1 (MQ=255)
            gCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTc                                                                                                                                                                                                                                                                       >  1:727978/1‑300 (MQ=255)
                   aTCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATAAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAAc                                                                                                                                                                                                                                                                <  1:281619/300‑1 (MQ=255)
                            atatAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAgg                                                                                                                                                                                                                                                       >  1:246775/1‑300 (MQ=255)
                             tataGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGa                                                                                                                                                                                                                                                      <  2:415922/300‑1 (MQ=255)
                                 gACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTg                                                                                                                                                                                                                                                  >  2:457202/1‑300 (MQ=255)
                                     ccTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAAc                                                                                                                                                                                                                                              >  1:643165/1‑300 (MQ=255)
                                      cTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGNGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACg                                                                                                                                                                                                                                             >  1:485318/1‑300 (MQ=255)
                                      cTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACg                                                                                                                                                                                                                                             >  1:300179/1‑300 (MQ=255)
                                                gTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAAc                                                                                                                                                                                                                                   >  1:440883/1‑300 (MQ=255)
                                                        tCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGaa                                                                                                                                                                                                                           >  2:662458/1‑300 (MQ=255)
                                                         ccccAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGaat                                                                                                                                                                                                                          >  1:347241/1‑300 (MQ=255)
                                                         ccccAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCAGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGaat                                                                                                                                                                                                                          >  1:249408/1‑300 (MQ=255)
                                                         ccccAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGACCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCAATGAAGCTGCCGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCAGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGaat                                                                                                                                                                                                                          >  2:199844/1‑300 (MQ=255)
                                                           ccAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGaataa                                                                                                                                                                                                                        >  1:301754/1‑300 (MQ=255)
                                                                            ccGGTCCGCCTGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCa                                                                                                                                                                                                       <  1:699302/300‑1 (MQ=255)
                                                                                  cgccgGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCa                                                                                                                                                                                                 >  1:401694/1‑300 (MQ=255)
                                                                                     cgGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGc                                                                                                                                                                                              >  1:226228/1‑300 (MQ=255)
                                                                                            gCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGAAAAGCTTACCGCGCTCGAACAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCCGGAAtt                                                                                                                                                                                       >  1:350328/1‑300 (MQ=255)
                                                                                                                               tCGATGGAGCCCACGACACCATGACCGCGCTCGACCAGCTTCATGCCGAGGCGACACTTGCAGTTCTAAGGGTTCTGATCGATATAGCAGTGACGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCACCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACACACGTTCAACAGACTGACTAATCGTCATAATACTGGCATGTCCACGCTGTAATTAAGGCgcccccgtccctctttcgggctgcaacatc                                                                                                                                                    >  1:619017/1‑271 (MQ=255)
                                                                                                                                          cACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGa                                                                                                                                         >  1:4464/1‑300 (MQ=255)
                                                                                                                                                               gACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCAGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAGTGCTGGCATGTACACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTctccctctacct                                                                                                                    >  2:407615/1‑300 (MQ=255)
                                                                                                                                                                aCCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGGTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtg                                                                                                                   <  2:411188/300‑1 (MQ=255)
                                                                                                                                                                  cAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtgtg                                                                                                                 <  1:225228/300‑1 (MQ=255)
                                                                                                                                                                    gCTTCATGCCTAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGTAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGTTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTggg                                                                                                               <  2:408941/300‑1 (MQ=255)
                                                                                                                                                                    gCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTACCTGTggg                                                                                                               >  1:586796/1‑300 (MQ=255)
                                                                                                                                                                    gCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTCTTTCGGGATGAAAAATCAATGAAATGAAGCCGGTGTTCTCCCTCTCCCTGTggg                                                                                                               >  2:252288/1‑300 (MQ=255)
                                                                                                                                                                        cATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGagag                                                                                                           <  1:642305/300‑1 (MQ=255)
                                                                                                                                                                                ggCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCgggg                                                                                                   <  1:674118/300‑1 (MQ=255)
                                                                                                                                                                                            cGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGTTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCTGGGGTGAGGGCACCaa                                                                                       <  1:466056/300‑1 (MQ=255)
                                                                                                                                                                                                         cTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCAGGGGTGAGGGCACCAACGCGCAGCGGAcc                                                                          >  1:241081/1‑300 (MQ=255)
                                                                                                                                                                                                             tCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAAt                                                                      <  1:593331/300‑1 (MQ=255)
                                                                                                                                                                                                              cGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATc                                                                     >  1:228475/1‑300 (MQ=255)
                                                                                                                                                                                                                atatAGCCGTGAAGCTTCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACGGAATAATCGGCATAATGCTGGCATGTCCACGCGGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCAc                                                                   <  1:733976/300‑1 (MQ=255)
                                                                                                                                                                                                                atatAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATTGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAGAATCAATGAAATGGAGCCGGTGTTCTACCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCCATCAc                                                                   >  1:96080/1‑300 (MQ=255)
                                                                                                                                                                                                                atatAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACTCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCag                                                                   >  2:364500/1‑299 (MQ=255)
                                                                                                                                                                                                                atatAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCAc                                                                   >  1:136074/1‑300 (MQ=255)
                                                                                                                                                                                                                   taGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTcc                                                                >  1:81218/1‑300 (MQ=255)
                                                                                                                                                                                                                        gTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGNGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAg                                                           <  1:524545/300‑1 (MQ=255)
                                                                                                                                                                                                                        gTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAg                                                           <  2:661924/300‑1 (MQ=255)
                                                                                                                                                                                                                        gTGAAGCTGCAGGGTTTTGAGCAGCGAGTGGAGAGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTCTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAg                                                           <  2:661923/300‑1 (MQ=255)
                                                                                                                                                                                                                          gAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCg                                                         <  1:382397/300‑1 (MQ=255)
                                                                                                                                                                                                                          gAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGGCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAGACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCg                                                         <  2:57930/300‑1 (MQ=255)
                                                                                                                                                                                                                             gggggggggTGTTTGGCAGCGAGGGGAGGGGATTCTTGCGCAGCCTCAGCAGTGTGCTGAGGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGTATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCGTGTCCACGCTGGAATTAGGGCGAAAACGTACCTTTTTCGGTATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTg                                                      <  2:142526/294‑1 (MQ=255)
                                                                                                                                                                                                                                     gTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGTTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGNTCAACAGACTGAATAATCGGCATAATGCTTGCATGTCCACGCTGGAATTAAGGCGAAAACGNACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCatcatc                                              <  2:578305/300‑1 (MQ=255)
                                                                                                                                                                                                                                     gTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCatcatc                                              >  1:358410/1‑300 (MQ=255)
                                                                                                                                                                                                                                       ttttAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGTTCGAGGATCTGCAACGCACGTTNAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTANCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCAc                                            <  2:570029/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  aggggAGGGTACTCTTGCTCAGCCCCACCAGTTTGCTGATGTCGGTGATCTTAAGCTCGTTTGCCTGCGCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCTGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATTAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTTGGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCg                                 <  2:381230/297‑1 (MQ=255)
                                                                                                                                                                                                                                                  aGTGGAGGGTACTCTTGCTCAGCCCCATCGGTTTGCTGGTGTCGTTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGTTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCTGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTTTTGTAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCg                                 <  2:380514/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  aGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGTTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCGGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCg                                 <  2:564379/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  aGTGGAGGGTACTCTTGCTCAGCCCCATCAGGTTGCGGATGTCGGTGATCTTGAGCTTGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACATACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCg                                 <  2:125697/300‑1 (MQ=255)
                                                                                                                                                                                                                                                     ggAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTAt                              >  1:276757/1‑300 (MQ=255)
                                                                                                                                                                                                                                                      gAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGTTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATg                             <  1:169349/300‑1 (MQ=255)
                                                                                                                                                                                                                                                          gTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATc                         <  1:430785/300‑1 (MQ=255)
                                                                                                                                                                                                                                                             ctctTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGCGGATTTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCGGGAATTAAGGCGAAAACATACCTTTTTCGGGATGAAAAACCAATGAAATGGAGCAGGTGTTCTCCCTCTACCTGTGGGAGAGAGCCGGGGTAAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGTGTTACCATCATCACCGACCTAATCATATGCATCCgg                      >  2:340329/1‑300 (MQ=255)
                                                                                                                                                                                                                                                             ctctTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCgg                      >  2:548345/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                       gCCCCATCAGTTTGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAGTGGAGCCGGTGTTCTCCCTCTACCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCCATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCgact            >  2:382597/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                                 tttGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCTGTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTATTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAacac  <  2:626097/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 tttGCTGATGTCGGTGATCTTGAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAacac  <  1:648803/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                      
GGCGGCCAGCTTGCCTTCAATCTTCTCGATATAGACCCCTTCACGCCCGTCCAGGATCCCCAGATGGGTGGTCTGCCCGGTCCGCCGGGACAGCTCCGTCAGCCAGCCTTTTGCCTTCTGCCGAATATCGATGGAGCCCACGACAAAATGGCCGCGCTCGACCAGCTTCATGCCGAGGCGATACTTGCCGTTCTCCGGGTTCTGATCGATATAGCCGTGAAGCTGCAGGGTTTTTAGCAGCGAGTGGAGGGTACTCTTGCTCAGCCCCATCAGTTTGCTGATGTCGGTGATCTTAAGCTCGGTGGCCTGCTCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACAC  >  NC_000913/288797‑289369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑