Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,315,259	C→T	G94S (GGC→AGC)	phnO ←	aminoalkylphosphonate N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,315,259	0	C	T	100.0%	200.0 / NA	58	G94S (GGC→AGC)	phnO	aminoalkylphosphonate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/27);  total (31/27)

TCACTACGCCGCTGCACGGTTGGCGGCGATACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCC  >  NC_000913/4314970‑4315549                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                   tCACTACGCCGCTGCACGGTTGGCGGCGATACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTAACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCACACGCCAGTAACTTACTGCTGACGTTCAGa                                                                                                                                                                                                                                                                                          >  2:237252/1‑300 (MQ=255) tCACTACGCCGCTGCACGGTTGGCGGCGATACTGCGGCGAGCGCCGCGCTCTGGCACAGGCCGCACACTCGCAGCCCCATGCCGGAACGCACTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGCAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTGGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCGGGCCTGGCGGGTTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGa                                                                                                                                                                                                                                                                                          >  2:29704/1‑300 (MQ=255)  cACTACGCCGCTGCACGGTTGGCGGCGATACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGAc                                                                                                                                                                                                                                                                                         <  2:312532/300‑1 (MQ=255)  cACTACGCCGCTGCACGGTTGGCGGCGATACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCACGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTCCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGAc                                                                                                                                                                                                                                                                                         <  2:664125/300‑1 (MQ=255)           gcTGCACGGTTGGCGGCGATACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCACTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCt                                                                                                                                                                                                                                                                                >  1:196527/1‑300 (MQ=255)                 cGGTTGGCGGCGATACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGTCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCa                                                                                                                                                                                                                                                                          <  2:662907/300‑1 (MQ=255)                   gTTGGCGGCGATACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCAtt                                                                                                                                                                                                                                                                        >  1:40817/1‑300 (MQ=255)                         ggcgATACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCGGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGGCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTGGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTaccacc                                                                                                                                                                                                                                                                  <  2:214052/300‑1 (MQ=255)                             aTACTGCGGCGATCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTTTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACt                                                                                                                                                                                                                                                              >  1:596771/1‑300 (MQ=255)                             aTACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAANCGGAAGTGGCTCTGCTCGTAGCCTTCNCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACt                                                                                                                                                                                                                                                              >  2:496805/1‑300 (MQ=255)                                                     ggCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGa                                                                                                                                                                                                                                      >  1:700810/1‑300 (MQ=255)                                                        gcAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACat                                                                                                                                                                                                                                   <  1:79162/300‑1 (MQ=255)                                                                        cAGCCCCATGCCGGAATGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCATGCTCATTTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGGTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTg                                                                                                                                                                                                                   <  2:26680/300‑1 (MQ=255)                                                                                         gCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCa                                                                                                                                                                                                  >  1:277125/1‑300 (MQ=255)                                                                                             tgtgCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGcc                                                                                                                                                                                              <  2:340147/300‑1 (MQ=255)                                                                                              gtgCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCg                                                                                                                                                                                             >  2:332195/1‑300 (MQ=255)                                                                                              gtgCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCg                                                                                                                                                                                             >  1:41127/1‑300 (MQ=255)                                                                                                         gtgccggtgAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGAGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTCACACCAACTCCTAACTTTCGTCGACCTAGTTGCCATAATGCAGATGAAACTGCAAATGCAGGCCGATTATGCCGagaaattagcc                                                                                                                                                                                  >  2:566673/1‑290 (MQ=255)                                                                                                                    cGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGCCATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAgcg                                                                                                                                                                       >  2:562929/1‑300 (MQ=255)                                                                                                                         gggTCAGGCTCATGTTAAAGCGTCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGCTCGGTCCTTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTACTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGCCACCTTCACCATCAAGCAGCGCCAGa                                                                                                                                                                  >  2:49914/1‑300 (MQ=255)                                                                                                                          ggTCATGCTCATGTTACAGCGCCTTGGTGAAGCGGAATTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGTTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGAt                                                                                                                                                                 <  2:341691/300‑1 (MQ=255)                                                                                                                          ggTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCNTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATNAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGAt                                                                                                                                                                 <  2:672536/300‑1 (MQ=255)                                                                                                                          ggTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGAt                                                                                                                                                                 <  1:85997/300‑1 (MQ=255)                                                                                                                             cAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTGCGCGCAGATAGAAACGGTGCGCTTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGt                                                                                                                                                              <  2:235741/300‑1 (MQ=255)                                                                                                                                  tCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGTGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTAGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTAACACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGTAGCGCCAGATGGTAgtgc                                                                                                                                                         >  2:138355/1‑297 (MQ=255)                                                                                                                                  tCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGTTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAgcgc                                                                                                                                                         <  1:543047/300‑1 (MQ=255)                                                                                                                                            gcgcCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGt                                                                                                                                               <  1:551529/300‑1 (MQ=255)                                                                                                                                            gcgcCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGGGCGCTTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACCTACTGCTGACGTTCAGACCGCGCGCCTGCGGNATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCNAGCAGCGCCAGATGGTAGCACATGTTTGGGt                                                                                                                                               <  2:504214/300‑1 (MQ=255)                                                                                                                                                      gAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTTGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGTTCGCGCAGAtt                                                                                                                                     <  2:701081/300‑1 (MQ=255)                                                                                                                                                        aGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTATTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACTGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAATAGCGCCAGATGGTAGCGCATGTGTGGGCCGCGCAGATTgg                                                                                                                                   >  2:358314/1‑300 (MQ=255)                                                                                                                                                                  gCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAAcc                                                                                                                         >  1:111907/1‑300 (MQ=255)                                                                                                                                                                       gCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCAcgc                                                                                                                    >  1:181095/1‑300 (MQ=255)                                                                                                                                                                        cTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCAcgcg                                                                                                                   >  1:664491/1‑300 (MQ=255)                                                                                                                                                                                         gATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCaa                                                                                                  >  1:34372/1‑300 (MQ=255)                                                                                                                                                                                           tAGAGACGGTGCGCGTCGTGGCGCTTCACGTTAGTCGAAAGTTCGGTCATTTCAGCCCCGGCCTGGCGGGCTTCTCCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCGGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAACGCATGTTTGGGTCGCACAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAAc                                                                                                <  2:107169/300‑1 (MQ=255)                                                                                                                                                                                            aGAAACGGTGCGCGTCTTGGCGCTTCACGTTGGTCGAAAGTTCGTTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACt                                                                                               <  2:300630/300‑1 (MQ=255)                                                                                                                                                                                             gAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTc                                                                                              <  1:298581/300‑1 (MQ=255)                                                                                                                                                                                                  ggTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTACTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACACATGGTGGCCAAACTCCgact                                                                                         >  2:140660/1‑297 (MQ=255)                                                                                                                                                                                                           cgtGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCt                                                                                <  1:216311/300‑1 (MQ=255)                                                                                                                                                                                                                                              tCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTAc                                             <  1:322839/300‑1 (MQ=255)                                                                                                                                                                                                                                                   cccGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGt                                        <  1:680649/300‑1 (MQ=255)                                                                                                                                                                                                                                                      ggCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTTACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGc                                     <  2:393533/300‑1 (MQ=255)                                                                                                                                                                                                                                                       gCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGcc                                    >  1:268934/1‑300 (MQ=255)                                                                                                                                                                                                                                                        ccTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCTTACGTTCAGACCGCGCGCCTGCTGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCg                                   <  2:101079/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 cttcttcttCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGNAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                          <  1:509563/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 cttcttcttCTGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt                          >  1:247189/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cttctGCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCNTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAg                    >  1:519115/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cctctGCCCACGCCAGTAATTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAGCTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAAATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACGGCGTGGCCGGGCGAAGCTCACAAg                    <  2:380760/298‑1 (MQ=255)                                                                                                                                                                                                                                                                          aggCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAg                 <  2:707146/298‑1 (MQ=255)                                                                                                                                                                                                                                                                            gCCCACGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAgct               >  1:723618/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                 cGCCAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgt          >  1:576292/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   ccattAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgtgt        >  1:122624/5‑300 (MQ=255)                                                                                                                                                                                                                                                                                   ccAGTAACTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGgtgt        >  1:552540/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        aaCTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTTCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtctc   <  1:330697/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        aaCTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCAGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTCAACCGCGTCGGTGTCGTACTGCGCGGCCGGGCGAAGCTCACAAGCAGACATGGTGTTtctc   >  1:189202/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         aCTTACTGCTGACGTTGAGACCGCACGCCTGCGGCATTACCACCAACTCCTGAACTTCGTCGATCCAGCTGACATGATACAAATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGACATCAAGCAGCGCCAGATGGTAGCGCATGTTTGAGTCGCGCAGATTGGCGTTACAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCATGTTTTAGCTCACAAATCAGCGCGTAAACCGCATCGGTATCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTGTCTcc  >  2:40944/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         aCTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTtctac  >  2:693811/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                         aCTTACTGCTGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGTCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTcc  >  1:461136/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                   TCACTACGCCGCTGCACGGTTGGCGGCGATACTGCGGCGAGCGCCGCGCTCTGGCGCAGGCCGCACACTCGCAGCCCCATGCCGGAACGCCCTGTGCGCCGCCGGTGCCGGTGAGCGTGAGGGTCAGGCTCATGTTACAGCGCCTTGGTGAAGCGGAAGTGGCTCTGCTCGTAGCCTTCGCGCAGATAGAAACGGTGCGCGTCGTGGCGCTTCACGTTGGTCGAAAGTTCGGTCATTTCGGCCCCGGCCTGGCGGGCTTCTTCTTCTGCCCACGCCAGTAACTTACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAGCAGGCATGGTGTTTCTCC  >  NC_000913/4314970‑4315549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑