Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,516,350	G→A	P110S (CCA→TCA)	fecA ←	ferric citrate outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,516,350	0	G	A	100.0%	200.2 / NA	57	P110S (CCA→TCA)	fecA	ferric citrate outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/32);  total (25/32)

ACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAACC  >  NC_000913/4516054‑4516639                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   aCCGTAAGGGGCGAAGGGGCAGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGACAGGTCGTGGCTGCCGGTGCCGTTGTTTNCCGGCGCGCTGACGCCAGGGATGCGGNTAAGTACCTAACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCAAGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCAACCAGCCAGTCGCCGACCACGGTCCGGGCATCTTCTTTtgctgc                                                                                                                                                                                                                                                                                                 >  2:475055/1‑300 (MQ=255) aCCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATgc                                                                                                                                                                                                                                                                                                 >  2:137293/1‑300 (MQ=255)                   gACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGt                                                                                                                                                                                                                                                                               <  1:17082/300‑1 (MQ=255)                                       ggACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCg                                                                                                                                                                                                                                                           >  1:110037/1‑300 (MQ=255)                                               gAGCGGCTGGCTAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCTGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTAcc                                                                                                                                                                                                                                                   <  2:304855/300‑1 (MQ=255)                                               gAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTAcc                                                                                                                                                                                                                                                   <  1:584145/300‑1 (MQ=255)                                                aGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCt                                                                                                                                                                                                                                                  <  1:369733/300‑1 (MQ=255)                                                            ggCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGc                                                                                                                                                                                                                                      >  1:730910/1‑300 (MQ=255)                                                            gccgcttgTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGTTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCACCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGAGGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGc                                                                                                                                                                                                                                      <  2:44539/293‑1 (MQ=255)                                                                   gTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCATTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCcag                                                                                                                                                                                                                               <  1:270970/300‑1 (MQ=255)                                                                                                    ttcttGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTTACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATc                                                                                                                                                                                              <  1:415942/296‑1 (MQ=255)                                                                                                    gTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCCTGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCAAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTAATTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATc                                                                                                                                                                                              >  2:377826/1‑300 (MQ=255)                                                                                                    gTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATc                                                                                                                                                                                              <  1:544869/300‑1 (MQ=255)                                                                                                    gTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGAAGTCGTAATc                                                                                                                                                                                              >  2:366553/1‑300 (MQ=255)                                                                                                        tgtcTGCCGGTGCCGTTGTTTTCCTGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGTATGGTGGTTGCGCCGGTTTTGGCGAAACCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCCTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGTTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCg                                                                                                                                                                                          <  2:430877/297‑1 (MQ=255)                                                                                                                ggTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGcc                                                                                                                                                                                  <  1:642389/300‑1 (MQ=255)                                                                                                                    ccattgttTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTAGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGTGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCAGTGTAGGCCGTTg                                                                                                                                                                              >  2:135378/4‑300 (MQ=255)                                                                                                                                          cGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGc                                                                                                                                                        >  1:290966/1‑300 (MQ=255)                                                                                                                                                    gCGGTTAAGTACCTCACGCATGGTGTTTGCGCCGGTTTTGGCGAAATCCTCACGNCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGTGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACNTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAc                                                                                                                                              <  2:680223/300‑1 (MQ=255)                                                                                                                                                    gCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAc                                                                                                                                              >  1:312279/1‑300 (MQ=255)                                                                                                                                                     cgattAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTAAGGGCATCTTATTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCGGTTGTTGCAGGCCGCTCTCGAAGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAct                                                                                                                                             >  2:132122/4‑299 (MQ=255)                                                                                                                                                     cGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAAcc                                                                                                                                             >  1:59602/1‑300 (MQ=255)                                                                                                                                                      ggTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCg                                                                                                                                            <  2:717169/300‑1 (MQ=255)                                                                                                                                                       gTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCga                                                                                                                                           >  1:557437/1‑300 (MQ=255)                                                                                                                                                                      cATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCt                                                                                                                            >  1:294081/1‑300 (MQ=255)                                                                                                                                                                        tggtggTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCtgt                                                                                                                          >  1:314887/1‑300 (MQ=255)                                                                                                                                                                          gtggtTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCtgtgt                                                                                                                        >  1:549880/1‑300 (MQ=255)                                                                                                                                                                               tGCGCCGTTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCTCATCACCCAGCCATTCTCCTACCACGTTCAGTGCATCCTCTTTTTATGCGGGCGCGGGCTCCAGCGTCCAGCTGGGACCTCATAGAGGTTTTACGGGCAGGTAGATGTTGGTAAGAGGTGGTTGCAGGACGCTTTCGGCGGTGTAGTCGTCGTGCGGTTCGTGGCGTTGATTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGc                                                                                                                   <  1:184451/300‑1 (MQ=255)                                                                                                                                                                               tGCGCCGGTTTTGTCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGc                                                                                                                   <  1:561217/300‑1 (MQ=255)                                                                                                                                                                               tGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACTTCACGCGCGCCAGCATTTTCAAATACTTCGTTTTCACGCGCATCACTCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCAGGCGCTGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGGCCGCTGCCGTCCAGCAGTTGTTGCATGCCGCTCTCGACGTCGTAACCGCCATGCAGGCCGTTGCTCTGCTTGCCGCGCGGCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGc                                                                                                                   <  2:103415/300‑1 (MQ=255)                                                                                                                                                                               tGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGc                                                                                                                   <  2:109655/300‑1 (MQ=255)                                                                                                                                                                               tGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAACATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGc                                                                                                                   <  2:409721/300‑1 (MQ=255)                                                                                                                                                                                        tttGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTNTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGAtt                                                                                                          <  1:528535/300‑1 (MQ=255)                                                                                                                                                                                           ggCGAANTCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCNTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCATAATCGCCGTGCATGCCGTTGCTCTGCTTGCCGCGCGTCAGGACGGCGGCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAg                                                                                                       <  2:605318/300‑1 (MQ=255)                                                                                                                                                                                              gAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAgcgc                                                                                                    <  1:1487/300‑1 (MQ=255)                                                                                                                                                                                                     tCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTGCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCACTCTCGACGTCGTAATCGCCGTGCAGGCCGTTACTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCg                                                                                             <  2:424152/300‑1 (MQ=255)                                                                                                                                                                                                                  cgtcacgCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTg                                                                                >  1:637261/1‑300 (MQ=255)                                                                                                                                                                                                                           cgcCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTa‑a                                                                      >  1:46710/1‑300 (MQ=255)                                                                                                                                                                                                                           cgcCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTCCCCCGCAGTCTGCTGCCGTCCCTCAGTTGTTGCAGGCCGCTCTCAACATCGCAATCGCCGTGCAGGCAGTTGCTCTGCTTGCCGCGCGCCAGGCTGGCGTCAACCGGGAGGGTAGCTCCGCTGTGTGCAGAATAGTGGGTGAGCGCTTTATTGAGCGATCCAGGTGCGATATTa‑a                                                                      >  2:717383/1‑300 (MQ=255)                                                                                                                                                                                                                             ccAGCATGTTCAAATACGTCGTTTTCGCGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCAGGCTCCAGTGTCCAGCTGTTATTTCCCATCGGTTTTACCTGCAGTNCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCCTTGCTCTACTTGCCGCGCGTCAGGCTGGCGCCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑Acc                                                                    <  2:172391/300‑1 (MQ=255)                                                                                                                                                                                                                             ccAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATNTCCCATCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑Acc                                                                    <  1:484854/300‑1 (MQ=255)                                                                                                                                                                                                                             ccAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATNTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑Acc                                                                    <  1:520155/300‑1 (MQ=255)                                                                                                                                                                                                                               aGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGCTTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCtg                                                                  >  1:106866/1‑300 (MQ=255)                                                                                                                                                                                                                                    gTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGGATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCtgtgtcg                                                             >  2:276730/1‑297 (MQ=255)                                                                                                                                                                                                                                       cAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCATGGTCAGGGCATCTTCTTTTGATGCGGGCGTGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGTCCGCTCTCGACTTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCCGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCaa                                                          <  2:699811/300‑1 (MQ=255)                                                                                                                                                                                                                                       cAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCaa                                                          <  1:598937/300‑1 (MQ=255)                                                                                                                                                                                                                                               tcgtTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTACCGAGCGATCCCGGTGCGATATTC‑ACATGTGAAGCAAAAGCGGac                                                  >  2:717864/1‑299 (MQ=255)                                                                                                                                                                                                                                                           gcATCAACCAGCCAGTCGCCGACCACGGTCAGGACATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGTAGTCCGCTGCCGTCCAGCAGTTGTTGCCGGCCGCTCTCGGCGTCGTAATCGCCGTACAGGCCGTTGCTCTGCTTGCCGCGCGTCGGGCTGGCGTCAAACGAGGGGGTAAATCCGCGGTGTGAGGCATACGGATGGAGCGCTTTATCTAGCGATCCCGGTGCGAGATTACACC‑GCGCAGCAAAAGCGGagaaagggggaccg                                      >  2:250279/1‑287 (MQ=255)                                                                                                                                                                                                                                                              tCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATAATGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGAGGAAAACGAGAGACCGGcc                                   >  2:611958/1‑300 (MQ=255)                                                                                                                                                                                                                                                              tCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGACCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGAGATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACTGGca                                   >  2:260951/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                aCCACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCCGGCCGTTGCCCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGACATTC‑ACCTATTCAGCAGACGCGGAAACCGCAAGACCAGCCAACGTCAGAAGGCTccga                 >  2:207242/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                  cACGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCg               <  1:586132/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    cGGTCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGaa             >  1:667482/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       tCAGGGCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATgg          <  1:620001/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gggCATCTTCTTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGgtgt       <  1:18456/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               tcttctTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCATCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCTAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAAcc  <  1:341952/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               tcttctTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAAcc  <  1:446036/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               tcttctTTTGATGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAAGGGTGGTAAcc  <  1:729249/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                   ACCGTAAGGGGCGAAGGGGACGGGGATGCCGTCCATCAGGACGGTCGAGCGGCTGGCGAGGCGCGGGTTCAGGCCCCGGATGCCAAAGTTCATCGCCAGGTCGTGGCTGCCGGTGCCGTTGTTTTCCGGCGCGCTGACGCCAGGGATGCGGTTAAGTACCTCACGCATGGTGGTTGCGCCGGTTTTGGCGAAATCCTCACGGCGGATCACGTCACGCGCGCCAGCATGTTCAAATACGTCGTTTTCACGCGCATCACCCAGCCAGTCGCCGACCACGGTCAGGGCATCTTCTTTTGGTGCGGGCGCGGGCTCCAGCGTCCAGCTGTTATTTCCCAGCGGTTTTACCTGCAGTCCGCTGCCGTCCAGCAGTTGTTGCAGGCCGCTCTCGACGTCGTAATCGCCGTGCAGGCCGTTGCTCTGCTTGCCGCGCGTCAGGCTGGCGTCAACCGAGAGGGTAAATCCGCTGTGTGCGGCATACTGATTGAGCGCTTTATCGAGCGATCCCGGTGCGATATTA‑ACCTGTGCAGCAAAAGCGGAAAACGAGAGACCGGCCAGCGGCAGCAGGCTCAGGCGAATGGTGTTAACC  >  NC_000913/4516054‑4516639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑