breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,645 G→A W282* (TGG→TAG)  thrB → homoserine kinase
RA 71,170 T→C S262P (TCG→CCG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 81,102 G→A S286L (TCG→TTG)  leuB ← 3‑isopropylmalate dehydrogenase
RA 110,258 C→T Y660Y (TAC→TAT secA → protein translocation ATPase
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
MC JC 255,892 Δ18,063 bp IS5‑mediated gpt[ykfC] 26 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 292,019 C→T L174L (CTA→TTA)  yagJ → protein YagJ
RA 298,885 G→A P684S (CCG→TCG)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
RA 321,260 A→T intergenic (+179/‑348) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
RA 365,306 G→A R334C (CGC→TGC)  lacZ ← beta‑galactosidase
RA 395,169 C→T E373E (GAG→GAA ampH ← peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
RA 400,444 G→A T160T (ACC→ACT ddlA ← D‑alanine‑‑D‑alanine ligase A
RA 410,666 C→T P175S (CCT→TCT)  mak → fructokinase
RA 414,163 G→A R531* (CGA→TGA)  sbcC ← ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit
RA 416,276 G→A A226V (GCA→GTA)  sbcD ← ATP‑dependent structure‑specific DNA nuclease ‑ SbcD subunit
RA 420,423 G→A G278D (GGC→GAC)  brnQ → branched chain amino acid transporter BrnQ
RA 421,027 C→T R14R (CGC→CGT proY → putative transporter ProY
RA 431,912 C→T W34* (TGG→TGA tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 465,005 G→A V181V (GTC→GTT queC ← 7‑cyano‑7‑deazaguanine synthase
RA 467,736 G→A G109S (GGT→AGT)  cof → HMP‑PP phosphatase
RA 471,709 G→A K358K (AAG→AAA mdlB → ABC transporter family protein MdlB
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 495,680 C→T F187F (TTC→TTT htpG → chaperone protein HtpG
RA 534,742 G→A G276D (GGT→GAT)  gcl → glyoxylate carboligase
RA 537,024 C→T P147S (CCG→TCG)  glxR → tartronate semialdehyde reductase 2
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 585,712 T→G intergenic (‑79/+435) ompT ← / ← envY omptin family outer membrane protease OmpT/DNA‑binding transcriptional activator EnvY
RA 627,348 G→A V427M (GTG→ATG)  entE → 2,3‑dihydroxybenzoate‑AMP ligase
MC JC 677,815 Δ10,036 bp IS5‑mediated [djlB]gltI [djlB], ybeT, ybeU, djlC, hscC, rihA, gltL, gltK, gltJ, sroC, gltI
RA 694,941 G→A L5L (CTC→CTT miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 708,774 G→A A147A (GCG→GCA chiP → chitooligosaccharide outer membrane channel
RA 721,604 G→A R44C (CGT→TGT)  kdpE ← DNA‑binding transcriptional activator KdpE
RA 753,977 G→A R164R (CGC→CGT gltA ← citrate synthase
RA 758,462 G→A intergenic (+57/‑244) sdhB → / → sucA succinate:quinone oxidoreductase, iron‑sulfur cluster binding protein/subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 780,624 C→T noncoding (71/76 nt) lysT → tRNA‑Lys
RA 789,580 C→A E134* (GAA→TAA)  galK ← galactokinase
RA 822,527 C→T E405K (GAA→AAA)  clsB ← cardiolipin synthase B
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 908,169 C→T R38R (AGG→AGA ybjT ← putative NAD(P)‑binding protein YbjT
RA 1,038,477 2 bp→AA coding (738‑739/1545 nt) appC → cytochrome bd‑II subunit 1
RA 1,052,001 C→T T52I (ACC→ATC)  ymcE → protein YmcE
RA 1,097,037 (G)7→8 intergenic (+208/+528) ycdU → / ← serX uncharacterized protein YcdU/tRNA‑Ser
JC JC 1,105,699 IS1 (+) +9 bp coding (286‑294/312 nt) ymdA → uncharacterized protein YmdA
RA 1,123,823 2 bp→AG coding (231‑232/648 nt) grxB ← reduced glutaredoxin 2
RA 1,147,792 T→A S58T (TCG→ACG)  plsX → putative phosphate acyltransferase
RA 1,153,007 G→A A357T (GCG→ACG)  fabF → 3‑oxoacyl‑[acyl carrier protein] synthase 2
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,212,088 (C)8→7 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM/protein YmgK
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,308,684 T→C intergenic (‑39/‑104) clsA ← / → yciY cardiolipin synthase A/uncharacterized protein YciY
RA 1,331,059 2 bp→AT coding (12‑13/2598 nt) topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,395,367 G→A R186C (CGT→TGT)  ynaI ← small conductance mechanosensitive channel YnaI
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
MC JC 1,411,925 Δ23,060 bp [ttcA]ynaM 34 genes
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,517,587 2 bp→TT intergenic (+25/‑60) ortT → / → ydcY orphan toxin OrtT/DUF2526 domain‑containing protein YdcY
RA 1,522,583 C→T Q161Q (CAG→CAA yncD ← putative TonB‑dependent outer membrane receptor
RA 1,541,037 A→G I518I (ATT→ATC narZ ← nitrate reductase Z subunit alpha
RA 1,541,058 G→A T511T (ACC→ACT narZ ← nitrate reductase Z subunit alpha
RA 1,591,711 C→A E156* (GAG→TAG)  hipA ← serine/threonine‑protein kinase toxin HipA
RA 1,600,081 C→T W43* (TGG→TGA lsrK ← autoinducer‑2 kinase
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,660,102 C→T N678N (AAC→AAT ynfE → putative selenate reductase YnfE
RA 1,664,866 C→T Q121* (CAA→TAA)  dmsD → redox enzyme maturation protein DmsD
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,712,661 A→T intergenic (+503/‑108) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,792,541 C→T G64E (GGA→GAA)  nlpC ← NlpC/P60 family lipoprotein NlpC
RA 1,799,792 C→T intergenic (‑43/+10) rplT ← / ← rpmI 50S ribosomal subunit protein L20/50S ribosomal subunit protein L35
RA 1,836,397 G→A E109K (GAG→AAG)  ynjB → putative ABC transporter periplasmic binding protein YnjB
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,959,375 G→A P158S (CCG→TCG)  yecM ← putative metal‑binding enzyme YecM
RA 1,971,380 C→T E438K (GAA→AAA)  tar ← methyl‑accepting chemotaxis protein Tar
RA 1,979,987 C→T P79S (CCC→TCC)  uspC → universal stress protein C
RA 1,981,874 C→T G172S (GGT→AGT)  otsB ← trehalose‑6‑phosphate phosphatase
RA 1,987,212 A→G Q96Q (CAA→CAG ftnB → putative ferritin‑like protein
RA 1,993,143 G→A P522S (CCA→TCA)  uvrC ← UvrABC excision nuclease subunit C
RA 2,001,353 T→C Y146C (TAC→TGC)  fliA ← RNA polymerase sigma factor FliA
RA 2,002,825 C→T G261E (GGA→GAA)  fliC ← flagellar filament structural protein
RA 2,003,378 T→C T77A (ACC→GCC)  fliC ← flagellar filament structural protein
RA 2,021,904 C→T L12L (CTC→CTT fliP → flagellar biosynthesis protein FliP
RA 2,023,559 A→G T223A (ACT→GCT)  fliR → flagellar biosynthesis protein FliR
RA 2,026,636 C→T G461E (GGA→GAA)  dgcQ ← diguanylate cyclase DgcQ
RA 2,027,332 T→A E229V (GAA→GTA)  dgcQ ← diguanylate cyclase DgcQ
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
RA 2,053,034 C→T intergenic (+1020/‑609) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
RA 2,060,006 C→T Q303Q (CAG→CAA cbl ← DNA‑binding transcriptional activator Cbl
RA 2,064,731 C→T R164R (CGG→CGA cobS ← cobalamin 5'‑phosphate synthase
RA 2,066,671 C→T Q205Q (CAG→CAA insH6 ← IS5 family transposase and trans‑activator
RA 2,068,504 C→T intergenic (‑1219/‑131) insH6 ← / → yoeA IS5 family transposase and trans‑activator/TonB‑dependent receptor plug domain‑containing protein YoeA
RA 2,068,723 C→T pseudogene (89/306 nt) yoeA → TonB‑dependent receptor plug domain‑containing protein YoeA
RA 2,076,331 C→T L8L (CTC→CTT yeeS → RadC‑like JAB domain‑containing protein YeeS
RA 2,083,303 C→T A183V (GCG→GTG)  sbcB → exodeoxyribonuclease I
RA 2,090,780 C→T Q197* (CAA→TAA)  hisG → ATP phosphoribosyltransferase
RA 2,099,385 C→T V77I (GTC→ATC)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 2,104,024 G→T I162I (ATC→ATA wbbK ← putative glycosyltransferase WbbK
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,111,502 C→T G159R (GGA→AGA)  rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,112,656 C→T V136I (GTC→ATC)  rfbB ← dTDP‑glucose 4,6‑dehydratase 1
RA 2,120,969 G→A L196L (CTG→TTG)  wcaJ ← UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase
RA 2,128,046 C→T S90N (AGC→AAC)  gmd ← GDP‑mannose 4,6‑dehydratase
RA 2,135,082 C→T A191T (GCC→ACC)  wzc ← protein‑tyrosine kinase Wzc
RA 2,140,209 C→T Q468Q (CAG→CAA asmA ← putative assembly protein AsmA
RA 2,142,598 G→A L117L (CTC→CTT udk ← uridine/cytidine kinase
RA 2,143,767 C→T L168L (CTG→TTG)  dgcE → putative diguanylate cyclase DgcE
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
JC JC 2,239,316 IS5 (–) +4 bp intergenic (‑27/+31) mglA ← / ← mglB D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit/D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,242,247 C→T G241R (GGG→AGG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter
RA 2,361,210 C→G E69Q (GAA→CAA)  rhmD ← L‑rhamnonate dehydratase
RA 2,388,789 C→T P52L (CCG→CTG)  yfbO → uncharacterized protein YfbO
RA 2,404,402 G→A S71L (TCG→TTG)  nuoB ← NADH:quinone oxidoreductase subunit B
RA 2,481,608 A→G L190S (TTA→TCA)  emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,498,392 A→C intergenic (‑97/‑279) alaC ← / → pyrS glutamate‑‑pyruvate aminotransferase AlaC/sensor histidine kinase PyrS
RA 2,510,084 C→T S152F (TCT→TTT)  yfeO → putative transport protein YfeO
RA 2,518,927 C→T P39S (CCC→TCC)  yfeD → putative DNA‑binding transcriptional regulator YfeD
RA 2,520,199 A→G G158G (GGT→GGC gltX ← glutamate‑‑tRNA ligase
RA 2,522,441 G→A R13C (CGT→TGT)  xapR ← DNA‑binding transcriptional activator XapR
RA 2,524,090 C→T Q263Q (CAG→CAA xapA ← xanthosine phosphorylase
RA 2,528,150 C→T E4K (GAG→AAG)  ypeB ← PF12843 family protein YpeB
RA 2,533,306 C→T P300S (CCA→TCA)  cysK → cysteine synthase A
RA 2,535,491 C→T P476S (CCA→TCA)  ptsI → PTS enzyme I
RA 2,535,795 C→T intergenic (+2/‑39) ptsI → / → crr PTS enzyme I/Enzyme IIA(Glc)
RA 2,540,538 C→T D93N (GAC→AAC)  cysA ← sulfate/thiosulfate ABC transporter ATP binding subunit
RA 2,545,703 C→T intergenic (‑94/‑70) murR ← / → murQ DNA‑binding transcriptional dual regulator MurR/N‑acetylmuramic acid 6‑phosphate etherase
RA 2,546,804 T→A D44E (GAT→GAA murP → N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,567,007 G→A N367N (AAC→AAT eutH ← putative ethanolamine permease EutH
RA 2,570,269 G→A P23P (CCC→CCT eutJ ← putative ethanolamine utilization chaperonin EutJ
RA 2,577,291 C→T V363M (GTG→ATG)  maeB ← malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
RA 2,577,937 T→C E147E (GAA→GAG maeB ← malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
RA 2,585,210 G→A L106L (CTG→TTG)  aegA ← putative oxidoreductase AegA
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,605,751 G→A G314D (GGC→GAC)  hyfD → hydrogenase 4 component D
RA 2,605,853 G→A G348D (GGT→GAT)  hyfD → hydrogenase 4 component D
RA 2,607,337 G→A A141T (GCG→ACG)  hyfF → hydrogenase 4 component F
RA 2,710,250 G→A intergenic (‑86/‑170) rseD ← / → nadB rpoE leader peptide/L‑aspartate oxidase
RA 2,745,600 A→G Y107H (TAC→CAC)  rimM ← ribosome maturation factor RimM
RA 2,766,503 G→A D196N (GAT→AAT)  rnlA → RNase LS, toxin of the RnlAB toxin‑antitoxin system
RA 2,774,947 G→A G544S (GGT→AGT)  yfjW → uncharacterized protein YfjW
RA 2,818,449 T→C intergenic (‑175/+24) argY ← / ← argV tRNA‑Arg/tRNA‑Arg
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,880,635 T→C L138L (TTA→TTG casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
JC JC 2,955,094 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (905‑910/3543 nt) recB ← exodeoxyribonuclease V subunit RecB
RA 2,959,564 C→T W955* (TGG→TGA recC ← exodeoxyribonuclease V subunit RecC
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
RA 3,105,127 G→A G232E (GGA→GAA)  mltC → membrane‑bound lytic murein transglycosylase C
MC JC 3,131,341 Δ128 bp IS5‑mediated yghQ ← putative transport protein YghQ
RA 3,148,900 G→A G335D (GGC→GAC)  gpr → L‑glyceraldehyde 3‑phosphate reductase
RA 3,167,046 C→T A225T (GCA→ACA)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,245,992 G→A V257I (GTT→ATT)  exuT → hexuronate transporter
RA 3,256,301 G→A S117S (TCC→TCT cyuA ← putative L‑cysteine desulfidase CyuA
RA 3,279,276 C→G F121L (TTC→TTG kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,411,689 G→T E13* (GAA→TAA)  yhdJ → DNA adenine methyltransferase
RA 3,433,900 C→T L71L (CTA→TTA)  def → peptide deformylase
RA 3,441,062 G→A S205F (TCC→TTC)  rpsD ← 30S ribosomal subunit protein S4
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,486,175 G→A C19Y (TGC→TAC)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,489,288 G→A A298V (GCG→GTG)  argD ← N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase
RA 3,507,249 C→T D30N (GAC→AAC)  yhfT ← uncharacterized protein YhfT
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,522,616 C→T D46N (GAT→AAT)  hofM ← DNA utilization protein HofM
RA 3,526,801 C→T Y111Y (TAC→TAT igaA → inner membrane protein IgaA
RA 3,532,984 C→T A56V (GCC→GTC)  pck → phosphoenolpyruvate carboxykinase (ATP)
RA 3,555,047 2 bp→TG coding (1963‑1964/2706 nt) malT → DNA‑binding transcriptional activator MalT
RA 3,555,371 T→C F763L (TTT→CTT)  malT → DNA‑binding transcriptional activator MalT
RA 3,556,628 C→T Q74Q (CAG→CAA rtcA ← RNA 3'‑terminal phosphate cyclase
RA 3,560,362 G→A intergenic (+496/+260) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,563,956 C→T G17D (GGC→GAC)  yzgL ← putative uncharacterized protein YzgL
RA 3,566,962 C→T A358T (GCA→ACA)  glgA ← glycogen synthase
RA 3,578,319 C→T K136K (AAG→AAA gntR ← DNA‑binding transcriptional repressor GntR
RA 3,581,238 C→T P34L (CCT→CTT)  yhhY → N‑acetyltransferase YhhY
RA 3,602,271 C→T L159L (CTG→CTA ftsE ← cell division protein FtsE
RA 3,608,348 C→T A633V (GCG→GTG)  zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
RA 3,625,601 C→T intergenic (+87/+78) yhhI → / ← yhhJ putative transposase/ABC transporter family protein YhhJ
RA 3,632,568 C→T G8D (GGC→GAC)  yhiJ ← DUF4049 domain‑containing protein YhiJ
RA 3,633,558 C→T intergenic (‑968/‑1283) yhiJ ← / → yhiM DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM
RA 3,637,711 C→T L24L (CTG→TTG)  pitA → metal phosphate:H(+) symporter PitA
RA 3,646,970 G→T Q224H (CAG→CAT gor → glutathione reductase (NADPH)
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,648,339 C→T noncoding (46/82 nt) agrB → small regulatory RNA AgrB
RA 3,658,873 G→A G170S (GGT→AGT)  gadE → DNA‑binding transcriptional activator GadE
RA 3,660,853 G→A G147D (GGC→GAC)  mdtF → multidrug efflux pump RND permease MdtF
RA 3,661,547 G→A G378G (GGG→GGA mdtF → multidrug efflux pump RND permease MdtF
RA 3,679,581 G→A G55R (GGA→AGA)  kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,719,429 G→A intergenic (‑385/‑49) yiaF ← / → yiaG DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ
RA 3,730,560 C→T W69* (TGG→TAG)  xylA ← xylose isomerase
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,817,167 C→T T164T (ACC→ACT yicC → putative RNase adaptor protein YicC
RA 3,872,642 C→T W119* (TGG→TGA dgoD ← D‑galactonate dehydratase
RA 3,886,777 C→T intergenic (+55/‑51) yidC → / → mnmE membrane protein insertase YidC/5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
JC JC 3,890,222 IS5 (–) +4 bp intergenic (+77/‑11) tnaA → / → tnaB tryptophanase/tryptophan:H(+) symporter TnaB
RA 3,894,251 C→T S124F (TCC→TTC)  yieE → putative phosphopantetheinyl transferase
RA 3,895,856 C→T G252R (GGG→AGG)  adeP ← adenine:H(+) symporter
RA 3,897,452 C→T intergenic (+13/‑54) yieH → / → cbrB 6‑phosphogluconate phosphatase/putative inner membrane protein
RA 3,902,697 C→T W335* (TGG→TGA bglB ← 6‑phospho‑beta‑glucosidase B
RA 3,905,605 C→T intergenic (‑8/+126) bglF ← / ← bglG beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase/transcriptional antiterminator BglG
RA 3,906,597 C→T intergenic (‑30/+256) bglG ← / ← phoU transcriptional antiterminator BglG/negative regulator of the pho regulon
RA 3,910,955 C→T V191I (GTA→ATA)  pstS ← phosphate ABC transporter periplasmic binding protein
RA 3,914,574 C→T G209G (GGG→GGA glmU ← fused N‑acetylglucosamine‑1‑phosphate uridyltransferase and glucosamine‑1‑phosphate acetyltransferase
RA 3,919,381 G→A I159I (ATC→ATT atpA ← ATP synthase F1 complex subunit alpha
RA 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,931,346 G→A A11T (GCG→ACG)  kup → K(+):H(+) symporter Kup
RA 3,931,779 T→C F155S (TTT→TCT)  kup → K(+):H(+) symporter Kup
RA 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho
RA 3,968,403 G→A G163D (GGC→GAC)  rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 3,977,600 G→A A26T (GCG→ACG)  wecF → TDP‑N‑acetylfucosamine:lipid II N‑acetylfucosaminyltransferase
RA 3,983,718 G→A G254D (GGC→GAC)  aslB → putative anaerobic sulfatase maturation enzyme AslB
RA 3,998,071 G→A G30D (GGC→GAC)  uvrD → DNA helicase II
RA 4,000,778 G→A I93I (ATC→ATT yigE ← DUF2233 domain‑containing protein YigE
RA 4,005,075 G→A A72T (GCG→ACG)  pldA → outer membrane phospholipase A
RA 4,006,669 G→A G269E (GGG→GAG)  recQ → ATP‑dependent DNA helicase RecQ
RA 4,011,619 C→T L182L (CTG→TTG)  bioP → biotin transporter
RA 4,011,998 C→T Q273Q (CAG→CAA metR ← DNA‑binding transcriptional dual regulator MetR
RA 4,014,732 C→T C560C (TGC→TGT metE → cobalamin‑independent homocysteine transmethylase
RA 4,014,875 C→T P608L (CCG→CTG)  metE → cobalamin‑independent homocysteine transmethylase
MC JC 4,017,577 Δ408 bp coding (245‑652/1428 nt) rmuC → putative recombination limiting protein RmuC
RA 4,018,102 C→T A257V (GCC→GTC)  rmuC → putative recombination limiting protein RmuC
RA 4,026,650 G→A D42N (GAT→AAT)  fre → NAD(P)H‑flavin reductase
RA 4,029,121 C→T Q617Q (CAG→CAA fadB ← multifunctional enoyl‑CoA hydratase, 3‑hydroxyacyl‑CoA epimerase, Delta(3)‑cis‑ Delta(2)‑trans‑enoyl‑CoA isomerase, L‑3‑hydroxyacyl‑CoA dehydrogenase
RA 4,030,999 C→T intergenic (‑28/‑162) fadB ← / → pepQ multifunctional enoyl‑CoA hydratase, 3‑hydroxyacyl‑CoA epimerase, Delta(3)‑cis‑ Delta(2)‑trans‑enoyl‑CoA isomerase, L‑3‑hydroxyacyl‑CoA dehydrogenase/Xaa‑Pro dipeptidase
RA 4,038,003 C→T noncoding (485/2905 nt) rrlA → 23S ribosomal RNA
RA 4,046,693 C→T intergenic (‑91/‑273) yihG ← / → polA 1‑acylglycerol‑3‑phosphate O‑acyltransferase YihG/DNA polymerase I
RA 4,055,494 C→T R282R (CGG→CGA glnL ← protein histidine kinase NtrB
RA 4,067,865 C→T G471D (GGC→GAC)  yihQ ← sulfoquinovosidase
RA 4,073,072 C→T G167E (GGG→GAG)  yihU ← 3‑sulfolactaldehyde reductase
RA 4,078,683 C→T intergenic (+245/‑608) fabY → / → yiiE putative fatty acid biosynthesis enzyme FabY/putative DNA‑binding transcriptional regulator YiiE
RA 4,082,106 G→A G218G (GGC→GGT fdoH ← formate dehydrogenase O subunit beta
RA 4,095,498 C→T C162Y (TGC→TAC)  rhaA ← L‑rhamnose isomerase
RA 4,096,387 C→T R354R (CGG→CGA rhaB ← rhamnulokinase
JC JC 4,117,984 IS30 (+) +2 bp coding (106‑107/846 nt) glpF ← glycerol facilitator
RA 4,123,389 C→T intergenic (‑50/+42) ftsN ← / ← cytR cell division protein FtsN/DNA‑binding transcriptional repressor CytR
RA 4,126,368 C→T G148D (GGC→GAC)  priA ← primosomal protein N'
RA 4,131,075 A→G Q414R (CAA→CGA)  metL → fused aspartate kinase/homoserine dehydrogenase 2
RA 4,131,375 G→A R514H (CGC→CAC)  metL → fused aspartate kinase/homoserine dehydrogenase 2
RA 4,132,065 C→T A744V (GCC→GTC)  metL → fused aspartate kinase/homoserine dehydrogenase 2
RA 4,137,282 C→T E126K (GAA→AAA)  yijF ← DUF1287 domain‑containing protein YijF
RA 4,139,498 C→T E71K (GAA→AAA)  fsaB ← fructose‑6‑phosphate aldolase 2
RA 4,140,761 C→T R487R (CGG→CGA ptsA ← putative PTS multiphosphoryl transfer protein PtsA
RA 4,146,192 C→T A733V (GCC→GTC)  pflD → putative formate acetyltransferase 2
RA 4,150,410 C→T intergenic (‑145/+37) eptC ← / ← ppc phosphoethanolamine transferase EptC/phosphoenolpyruvate carboxylase
RA 4,153,836 G→A Q338* (CAA→TAA)  argE ← acetylornithine deacetylase
RA 4,154,616 C→T A78T (GCG→ACG)  argE ← acetylornithine deacetylase
RA 4,155,363 C→T T121T (ACC→ACT argC → N‑acetylglutamylphosphate reductase
RA 4,160,243 C→T G183D (GGT→GAT)  sthA ← soluble pyridine nucleotide transhydrogenase
RA 4,171,557 C→A intergenic (+13/‑80) rrlB → / → rrfB 23S ribosomal RNA/5S ribosomal RNA
RA 4,184,500 C→T P1086S (CCT→TCT)  rpoB → RNA polymerase subunit beta
RA 4,187,828 G→A E827K (GAG→AAG)  rpoC → RNA polymerase subunit beta'
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,215,670 G→T D65Y (GAT→TAT)  aceB → malate synthase A
RA 4,218,853 C→T Y86Y (TAC→TAT aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,224,762 G→A G312D (GGC→GAC)  metH → cobalamin‑dependent methionine synthase
RA 4,225,271 G→A V482I (GTA→ATA)  metH → cobalamin‑dependent methionine synthase
RA 4,261,296 C→T S233L (TCG→TTG)  yjbM → DUF2713 domain‑containing protein YjbM
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,306,660 G→A intergenic (‑63/+210) yjcS ← / ← alsK linear primary‑alkylsulfatase/D‑allose kinase
RA 4,316,498 G→A H241Y (CAC→TAC)  phnM ← RPnTP hydrolase
RA 4,322,048 (C)5→6 coding (375/726 nt) phnF ← putative transcriptional regulator PhnF
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
RA 4,452,986 C→T A139V (GCC→GTC)  ytfT → galactofuranose ABC transporter putative membrane subunit YtfT
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,479,944 G→A G72E (GGG→GAG)  yjgN → inner membrane protein YjgN
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
JC JC 4,586,822 IS5 (+) +4 bp intergenic (‑61/‑124) hsdR ← / → mrr type I restriction enzyme EcoKI endonuclease subunit/Type IV methyl‑directed restriction enzyme EcoKMrr

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 361657 362203 547 123 [0] [0] 129 [lacA]–[lacY] [lacA],[lacY]
* * ÷ NC_000913 803113 820913 17801 39 [37] [0] 128 [ybhI]–[ybhM] [ybhI],ybhJ,ybhC,ybhB,bioA,bioB,bioF,bioC,bioD,uvrB,ybhK,moaA,moaB,moaC,moaD,moaE,ybhL,[ybhM]
* * ÷ NC_000913 1196302 1211459 15158 38 [36] [36] 39 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978484 1979270–1978503 20–787 127 [0] [1] 149 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423802–3424461 3424461 1–660 38 [37] [37] 38 [rrlD] [rrlD]
* * ÷ NC_000913 4542050 4544734 2685 167 [0] [0] 118 [fimE]–[fimC] [fimE],fimA,fimI,[fimC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 19796 =NA (NA)122 (1.070) 75/596 0.2 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 361656 0 (0.000)coding (205/612 nt) lacA galactoside O‑acetyltransferase
* ? NC_000913 = 258675NA (NA)124 (1.090) 81/598 0.1 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 362204 = 0 (0.000)coding (976/1254 nt) lacY lactose permease
* ? NC_000913 273955 =NA (NA)128 (1.120) 92/598 0.0 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 820914 = 0 (0.000)coding (122/714 nt) ybhM Bax1‑I family protein YbhM
* ? NC_000913 = 275149NA (NA)49 (0.430) 39/598 1.8 49.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 297512 = 51 (0.450)coding (280/411 nt) yagP putative LysR family substrate binding domain‑containing protein YagP
* ? NC_000913 = 275149NA (NA)60 (0.530) 44/598 1.4 49.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 800228 62 (0.540)coding (348/954 nt) ybhD putative DNA‑binding transcriptional regulator YbhD
* ? NC_000913 1287362 =51 (0.450)46 (0.430) 36/558 1.8 51.8% intergenic (‑34/+176) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
?NC_000913 = 1287536 38 (0.360)intergenic (‑208/+2) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
* ? NC_000913 = 12994981 (0.010)128 (1.140) 78/590 0.1 99.6% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19784830 (0.000)127 (1.110) 82/598 0.1 100% intergenic (‑286/+35) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 1979271 =1 (0.010)139 (1.280)
+TTAAGTAATTGAGTG
80/568 0.1 99.3% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 2996360 = NA (NA)intergenic (+2504/+12) ygeI/insD4 protein YgeI/IS2 insertion element protein InsB
* ? NC_000913 3583428 =NA (NA)167 (1.470) 106/596 0.0 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 4542049 0 (0.000)coding (13/597 nt) fimE regulator for fimA
* ? NC_000913 = 3584195NA (NA)117 (1.020) 78/598 0.2 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 4544735 = 0 (0.000)coding (432/726 nt) fimC type 1 fimbriae periplasmic chaperone