Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,556,628	C→T	Q74Q (CAG→CAA)	rtcA ←	RNA 3'‑terminal phosphate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,556,628	0	C	T	100.0%	207.1 / NA	58	Q74Q (CAG→CAA)	rtcA	RNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/23);  total (35/23)

GGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGCTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTCCACCAGA  >  NC_000913/3556332‑3556904                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                     ggCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCAGGGCCGGAAGAGCAGACGTTGc                                                                                                                                                                                                                                                                                   >  1:591564/1‑300 (MQ=255)      gaCTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGAccc                                                                                                                                                                                                                                                                              >  1:251146/1‑300 (MQ=255)            cGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTc                                                                                                                                                                                                                                                                        <  2:580922/300‑1 (MQ=255)                           gCCTCCTGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCNATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGGGCCGCCGCTCACTTCAACACGCGAAGGTCTACCGGCAAACCAAAGCGCTGGCAGCATCGTTGGCAGCACCAGCGTACGAGTACCAGCGCTACCGAGAGCAAAGCCGTAATCGCCGCCGCGNACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGt                                                                                                                                                                                                                                                         <  2:829274/300‑1 (MQ=255)                             cTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTANTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCANGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTg                                                                                                                                                                                                                                                       <  2:824740/300‑1 (MQ=255)                                                   gCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTcgccgc                                                                                                                                                                                                                                 >  1:87570/1‑300 (MQ=255)                                                           gCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCTGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCTTCGCCGCCTTTACcg                                                                                                                                                                                                                         <  2:194291/300‑1 (MQ=255)                                                                      tgatgaATGCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGANAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAANTTCCGTCGCCGCCTTTACCGCGGTCAGAttc                                                                                                                                                                                                              <  2:645815/300‑3 (MQ=255)                                                                                                cGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTTCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCTCCTTTACCGCGGTCAGATGCTGGCGCAACAGCACAGGTTTcgcccg                                                                                                                                                                                    >  1:544598/1‑300 (MQ=255)                                                                                                cGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTcgcccg                                                                                                                                                                                    >  1:71922/1‑300 (MQ=255)                                                                                                   cTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGGGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCgcccgccc                                                                                                                                                                                 >  1:148046/1‑300 (MQ=255)                                                                                                   cTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCgcccgccc                                                                                                                                                                                 >  1:700390/1‑300 (MQ=255)                                                                                                        gCACCCGGCGGATAAAATCCGCAGGCGGCGCCGAAGGGTTATCGGTGCCGCCGCTCACTTCAAAACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGAGCACGGTGCAGGGACGGAAGAGCAGACGTTACGAACCAAGCTCCGCGCCTTCCACGGTTGTTCCGCAAATTTCCGTCGCCGACTTTACCGCGGTCAGATGCTGGCGCAACAGCCCAGGTTTCGCACGCACGGCAc                                                                                                                                                                            >  2:878383/1‑300 (MQ=255)                                                                                                              ggcggATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGc                                                                                                                                                                      <  1:675077/300‑1 (MQ=255)                                                                                                                        aTCCGCGGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACTCGAAGGTCCATCGGCAAACCACAACGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATggc                                                                                                                                                            <  2:291652/300‑2 (MQ=255)                                                                                                                        aTCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGt                                                                                                                                                            <  1:762588/300‑1 (MQ=255)                                                                                                                         tCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTa                                                                                                                                                           <  1:344335/300‑1 (MQ=255)                                                                                                                                   gcgcCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTCCCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCATGCCCGGCACGAATGCTGGTGATGGTAAAtggctggc                                                                                                                                                 >  2:369592/1‑300 (MQ=255)                                                                                                                                          cGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTNCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTat                                                                                                                                          >  1:655430/1‑300 (MQ=255)                                                                                                                                          cGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTNCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCAGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTat                                                                                                                                          >  1:657263/1‑300 (MQ=255)                                                                                                                                          cGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTat                                                                                                                                          >  2:348933/1‑300 (MQ=255)                                                                                                                                            ggTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTACGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTatca                                                                                                                                        >  2:159626/1‑300 (MQ=255)                                                                                                                                            ggTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTAACGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGTAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCATCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGATGGCCGGGTatca                                                                                                                                        >  2:4729/1‑300 (MQ=255)                                                                                                                                              ttATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCTGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTatcatc                                                                                                                                      <  2:37388/300‑1 (MQ=255)                                                                                                                                              ttATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTatcatc                                                                                                                                      <  1:351701/300‑1 (MQ=255)                                                                                                                                                            gcTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAgcgc                                                                                                                        >  2:21393/1‑300 (MQ=255)                                                                                                                                                                 cTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCNTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAgc                                                                                                                   >  1:611486/1‑300 (MQ=255)                                                                                                                                                                 cTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAgc                                                                                                                   >  1:104294/1‑300 (MQ=255)                                                                                                                                                                 cTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGAAAGGCTCAGCGCCGAgc                                                                                                                   >  2:514375/1‑300 (MQ=255)                                                                                                                                                                     aaCACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAg                                                                                                               >  1:425320/1‑300 (MQ=255)                                                                                                                                                                        acGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGAt                                                                                                            <  1:509136/300‑1 (MQ=255)                                                                                                                                                                        acGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGAt                                                                                                            <  2:554239/300‑1 (MQ=255)                                                                                                                                                                         cgcgAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCANACGTTGCGACCCCAGCTCCGCGCCTTNCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCAGGTTTCGCCCGCACGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATc                                                                                                           >  2:597231/1‑300 (MQ=255)                                                                                                                                                                         cgcgAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATc                                                                                                           >  1:335044/1‑300 (MQ=255)                                                                                                                                                                             aaGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGcc                                                                                                       <  1:823875/300‑1 (MQ=255)                                                                                                                                                                                           aaCCACCGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACGACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGccc                                                                                         <  2:756858/300‑1 (MQ=255)                                                                                                                                                                                                      gggCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATc                                                                              >  1:168303/1‑300 (MQ=255)                                                                                                                                                                                                       ggCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATcc                                                                             <  2:845512/300‑1 (MQ=255)                                                                                                                                                                                                       ggCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCAGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATcc                                                                             >  1:357740/1‑300 (MQ=255)                                                                                                                                                                                                           gcaCCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAgcg                                                                         >  1:283728/1‑300 (MQ=255)                                                                                                                                                                                                            caCCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAgcgc                                                                        <  1:728974/300‑1 (MQ=255)                                                                                                                                                                                                                   tGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGACGCCGCGCACGGTGCCGGGCAGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCACGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCAGGTTATCATCGCCAGGCTAAGCGCCGAGCGCAGGATCTGCACGCCACCGACGACCTGTGCGCCATCCAGCGACatcatc                                                                 >  2:53723/1‑300 (MQ=255)                                                                                                                                                                                                                       gcaCCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGNATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCttt                                                             >  1:598302/1‑300 (MQ=255)                                                                                                                                                                                                                       gcaCCAGCGTACAACTCCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCAGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCttt                                                             >  2:49348/1‑300 (MQ=255)                                                                                                                                                                                                                             gCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTNGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTtaatca                                                       >  1:645461/1‑300 (MQ=255)                                                                                                                                                                                                                             gCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTtcatca                                                       >  1:832049/1‑300 (MQ=255)                                                                                                                                                                                                                              cGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTcatcat                                                      >  1:174858/1‑300 (MQ=255)                                                                                                                                                                                                                                                          aaGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACNCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGTTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGc                          <  2:658549/300‑1 (MQ=255)                                                                                                                                                                                                                                                            gCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAg                        >  2:512156/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCAACTGACGCAg                        >  1:742813/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCNGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGt                     <  1:637998/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     gccgccgcGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCNGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGAt               <  1:613377/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      ccgccgcGCACGGTGCCGTGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGTTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGTTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATa              <  2:105373/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      ccgccgcGCACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATa              <  2:524347/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             gcACGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTcca       >  1:895494/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               aCGGTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTccacc     <  1:459445/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTCCACCAGa  >  1:43166/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gTGCCGGGCCGGAAGAGCAGACGTTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTCCAACAGa  >  2:541940/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                     GGCGAGACTTCCGTTGCCACCACACCGCCTCCGGCAGGGTAAAAACCGTGGCGTAACAGCGTGGTTTGCTGATGAATTCCTATTTTCGCCAGCAGCGGCTCCAGCACCCGGCGGATAAAATCCGCAGGCGGCGCCGACGGGTTATCGGTGCCGCCGCTCACTTCAACACGCGAAGGTCCATCGGCAAACCACAGCGCGGGCAGCACCGTTTGCAGCACCAGCGTACAACTTCCGGCGCTACCGATAGCAAAGCGGTAATCGCCGCCGCGCACGGTGCCGGGCCGGAAGAGCAGACGCTGCGACCCCAGCTCCGCGCCTTCCACGGTTGCTCCGCAAATTTCCGTCGCCGCCTTTACCGCGGTCAGATGCTGGCGCAACAGCCCCGGTTTCGCCCGCCCGGCACGAATGCTGGTGATGGTAAATGGCTGGCCGGTTATCATCGACAGGCTCAGCGCCGAGCGCAGGATCTGCCCGCCACCTTCGCCCTGTGCGCCATCCAGCGCAATCATCCTTTTCATCATTTATCCTTTTACGCACACCACCTGACGCAGGGTATAGATAACTTCCACCAGA  >  NC_000913/3556332‑3556904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑