Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,818,449	T→C	intergenic (‑175/+24)	argY ← / ← argV	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,449	0	T	C	89.7%	191.0 / 11.5	81	intergenic (‑175/+24)	argY/argV	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base C (50/20);  total (56/25)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01

ACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTTCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGTCAAACAAT  >  NC_000913/2818174‑2818740                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       actaAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAatat                                                                                                                                                                                                                                                                             >  1:580118/1‑300 (MQ=255)     aaGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCATGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTg                                                                                                                                                                                                                                                                         >  1:104707/1‑300 (MQ=255)       gCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGCAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCCAGTCAGCGGTGGTGCATCCGGGAAGCTTACTCGCCTTACGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TCTTAACGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTCGAATATGGTgtg                                                                                                                                                                                                                                                                       >  1:822103/1‑298 (MQ=21)       gCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CCGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAAGATTACTCGCCTTACGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTACA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCa                                                                                                                                                                                                                                                                       >  1:822756/1‑300 (MQ=255)               aaataagaaacgttGCATCCGGGAGGAGTCGAACTGCCTACTGCGCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGggag                                                                                                                                                                                                                                                               <  1:761719/287‑1 (MQ=18)               aaGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGggag                                                                                                                                                                                                                                                               >  1:98863/1‑300 (MQ=255)                        agaggcagtCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAAc                                                                                                                                                                                                                                                      <  2:61076/292‑1 (MQ=255)                                tCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTCCTTTACTTTCTG‑TTTTTTGCTCCCGCTCCCAAAGCCGTCTCACGTAGGAGCTGGTGCCTCCGGGGGGCTTACTCGCCTTGCGGCTCACCCTTCGGGCCTTTACTAAAGCAAATTTCTCCTCCCCGTTCCCTGTGGT‑CATTCCCTCCACCCCTTCCCTCCCTCCC‑TCACTG‑AA‑ATTCCGTAGAATATGGTGCATCCGGGAGGATTCGAACCtgcccct                                                                                                                                                                                                                                              >  1:61250/1‑294 (MQ=12)                                             gaccccncgccttcTCGTTTCGTAGCCGAGTACCCTATCCAGCTGAGCTACGGGNTCTTTGGGAAAACTTATTCTACTG‑CGGATATT‑‑GATACCGCTACTAAAGCCGTATCAAGTAATAGATGGTGCATCCGGGAGGATTACTCGCCGTCCGGCTCGCTCTGCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCGGCGGT‑TATTACCGCAACCTCTTCCAGACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                                                                <  2:740128/287‑1 (MQ=11)                                              cacttcaggccGCTCGGTTCGTATACGAGTACTCTATCCAGCCAAGCGACGGAGGCATCGGGAAA‑CTCACTTTACTG‑CAGAGTTGTTGATACCACGACTAAAGCCGTGTCAAGTAAGAGATGGGGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTCATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TTGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                                                                <  2:100669/291‑1 (MQ=17)                                              cacatccgaccgaccagTTCGTAGCCGAATACTCTGTCCAGCCTAGCTGCAGAGGCATCTGGAAA‑CCTGCTTTACTG‑CAGATGTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGNAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                                                                                                                                                                                                                                <  2:210906/284‑1 (MQ=18)                                                            tggTTTGTAGCCGAGGACTCCGTCCCGCTGGGCTACTGACGCATCGGGAAA‑CTTCCTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCATATCAATTAATCTATTGTACATCCGGGAGGATTCCTCGCCTTTCTTCTCTCCCTTCTGGCCGTTGCTAAAGCAACTTTATCCTCCAGGGTGACTTCGGT‑TGGTCCCGTAACTTTTTCCATGCCC‑CAGTTGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc                                                                                                                                                                                                                  <  1:436602/299‑1 (MQ=12)                                                              gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTG‑CTGATATT‑‑GATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTCCCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTATATAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGTTCGGTTCGTAGCCGAGTActctccccaga                                                                                                                                                                                                               >  1:521156/1‑293 (MQ=11)                                                              gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                                                                                                                                                                >  1:431185/1‑300 (MQ=255)                                                              gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCAACTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCAGAGTACTCTATCCAg                                                                                                                                                                                                                >  1:521170/1‑300 (MQ=255)                                                              gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCAACTCTTCCATACCTCCA‑GCGCTG‑CA‑AGTACGTAGAATATGGTGCANCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAATCTATCCAg                                                                                                                                                                                                                >  2:657628/1‑300 (MQ=255)                                                              gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCATGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                                                                                                                                                                >  1:228340/1‑300 (MQ=255)                                                                 cttagccgaataccccagccaacTGAGCTACGGGTGCATCGGGAAA‑CTCGCTTTATTG‑CAGATTTTTTGATACCGCTACTAAATCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                                                                                                                                                             <  1:374644/278‑1 (MQ=18)                                                                 cGTAGCCGAGTACTCTATCCAACTGAGCTACGGATGCATCGGGAAA‑CCTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                                                                                                                                                             <  2:98546/300‑1 (MQ=255)                                                                     gCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTAACGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCt                                                                                                                                                                                                         >  2:179350/1‑300 (MQ=255)                                                                            aCTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                                                                                                                                                                  >  1:779226/1‑300 (MQ=255)                                                                               ctATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGCTACCGCTACTACAGCCGTATCAAGTAGGGGATGGTGCATCTGGGAGGATTACTCGCCTTGTGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTCTCATCCCTGGTGCCTGCGGT‑TATTACCGCCGCTTCTTCCATACCTCCA‑TCGCTG‑AG‑AGTACGTAGGATGTGGTGCATCCGGGAGGATTCGACCCTCCGAACGCTCGGTTCGTAGCCGAGTAATCTATCCAGATGCGCTACGGCTGCaa                                                                                                                                                                                               >  2:373582/1‑300 (MQ=18)                                                                                    cAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTG‑CTGATATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAACGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCg                                                                                                                                                                                         >  1:61456/1‑300 (MQ=11)                                                                                         gAGCTACGGATGCATCGGGAAAACTTATTCTACTG‑CTGATATT‑‑GATACCGCTACTAAAGCCATATCAATTAAGAGATG‑TGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAgg                                                                                                                                                                                   >  1:503520/1‑300 (MQ=11)                                                                                                ggATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCgggg                                                                                                                                                                              >  1:448957/1‑300 (MQ=255)                                                                                                                     tttactG‑CAGATTTTTTGATACCACTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGTGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGc                                                                                                                                                          <  2:513062/300‑1 (MQ=255)                                                                                                                      attctactgcggatattGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGc                                                                                                                                                          <  2:513049/285‑1 (MQ=255)                                                                                                                                    tttGAGACAGCTACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATAc                                                                                                                                            <  1:181040/300‑1 (MQ=255)                                                                                                                                             gctacgacagccagcgtaggTAAGAGATGGCGCATACGGGAGGGTAACGTGGCTTGCGGCTCCGCCTTCGGGCCTTTATTAAGGAAGCGTGACACCCCCTGGTGCC‑GAGGTGTATTACCGCAACCTCTTTCATACCTCCA‑GCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTTAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCGTACTCCCTTAGc                                                                                                                                   <  2:191334/281‑1 (MQ=16)                                                                                                                                             gCTACTAAAGCCGTATCAAGTAATAGATGTTGCCTCCGGGAGGATTATTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTTTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGc                                                                                                                                   <  2:61249/300‑1 (MQ=255)                                                                                                                                             acgactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGc                                                                                                                                   <  2:61282/297‑1 (MQ=255)                                                                                                                                              ctaagaaaaccatATCAATTAAGAGATGGTGCATCCGTGAGGAATACTCGCCTTGCTGCTCGCCCTTCCTACCCTTGCTAAAGCAACGTTACCATCCCTAGTGCCTGCGGT‑TAATTCCTCTCTCTTTTCGCGACCCTCA‑TTGATGGAG‑ATTTCGTAGAAGATGGTGCATCCTGTAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGc                                                                                                                                   <  2:292465/288‑1 (MQ=2)                                                                                                                                                actaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCGAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAgg                                                                                                                                >  2:542084/1‑300 (MQ=255)                                                                                                                                                actaAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAgg                                                                                                                                >  1:754234/1‑300 (MQ=255)                                                                                                                                                  taAAGCCGTATCAAGTAATAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCTGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAgggg                                                                                                                              <  1:252565/300‑1 (MQ=255)                                                                                                                                                       cagtgtCAAGGAAGAGATGGGACATCCGGGAGAATGACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCGAAAGCAATGTTATCCTCCCTGGTGCCTGCGTT‑TATTACCACCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAgcgc                                                                                                                         <  1:159534/295‑1 (MQ=25)                                                                                                                                                             caattAAGAGATG‑TGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGc                                                                                                                  <  2:496609/296‑1 (MQ=255)                                                                                                                                                              aaggaaGAGATGGTGCATCCGGGAGGATGACTCTCTTGGTGGCTCTCCTTTCGGGCCGTTGCGAAAGAAACGTGATCCTCCCGGGTGCCGGCGGT‑GATTACCGCCACCTCTTCCGTACCTCCA‑TCGCTG‑AA‑AGTGCGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGc                                                                                                                  <  1:405175/296‑1 (MQ=25)                                                                                                                                                                gTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCct                                                                                                                >  2:531362/1‑300 (MQ=255)                                                                                                                                                                    gagaTGGTGCATCCGGGAGAATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCAGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCg                                                                                                            >  2:795246/1‑300 (MQ=255)                                                                                                                                                                              aTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTcac                                                                                                  >  1:740603/1‑300 (MQ=255)                                                                                                                                                                                 cGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TACTACCGCCACCTCTTCCATACCTCCA‑TCACTG‑AA‑AGTACGTAGAATATGGTGAATCCGGGGTGAGTCGAGCCTCCGACTACGCGGGTCGGAGCTGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTcaccac                                                                                               <  1:46738/300‑1 (MQ=25)                                                                                                                                                                                    gaggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACg                                                                                            <  1:886004/300‑1 (MQ=255)                                                                                                                                                                                      ggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTAGGCCACCTCACCACACGcc                                                                                          >  1:482569/1‑300 (MQ=255)                                                                                                                                                                                      ggaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCACTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAAGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCATTCAGCCTCTCGGCCACCTCACCACACGcc                                                                                          >  2:197610/1‑300 (MQ=255)                                                                                                                                                                                      ggaTTACTCGCCTTGCGGCTCGCACTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCAACTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTGCTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAACGCCTTCAGCCTCTCGGCCACCTCACCACACGcc                                                                                          >  2:197620/1‑300 (MQ=255)                                                                                                                                                                                             tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACg                                                                                   <  1:750525/300‑1 (MQ=255)                                                                                                                                                                                                ccTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGT‑TAATTCCGCTACCTCTTCGATACCTTCA‑TTGCTG‑AAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAg                                                                                 <  1:871692/300‑1 (MQ=17)                                                                                                                                                                                                           gCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACNGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGNGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCgaagaa                                                                     >  2:824844/1‑300 (MQ=255)                                                                                                                                                                                                            cccTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTATTACGAGTGCTTCGAAGAAc                                                                    >  1:489905/1‑300 (MQ=255)                                                                                                                                                                                                            cccTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCATGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAAc                                                                    >  2:421553/1‑300 (MQ=255)                                                                                                                                                                                                              cTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCNTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGANGAACtt                                                                  >  2:726477/1‑300 (MQ=255)                                                                                                                                                                                                              cTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCATGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTACA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTAGGCCACCTCACCACACGCCTCTTCCGAGTGCTTCGAAGAACtt                                                                  >  2:175935/1‑300 (MQ=255)                                                                                                                                                                                                                          gCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGT‑TAATTCCGCTACCTCTTCGATACCTTCA‑TTGCTG‑AAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGCGGCGGGGATTCGAACCCAAGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGTGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGCAGAACTTATTTATCGCTc                                                       >  1:885675/1‑300 (MQ=17)                                                                                                                                                                                                                          gCTAAAGCAACGTTATCATCCATAGTGCCTGCGGT‑TAATTCCGCTACCTCTTCGATACCTTCA‑TTGCTG‑AAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGAGGTGAGGTGGGGCTTCGAACCCAGGATGCAGCTTTTGACCGCATACTACATTAGCAGGGGAGCGCCTTAAGCCTCTAAGCCACCTCACCCCACGCCTCTTCCGAGTGCTTCGAAAAGCTCATTTATCGCTc                                                       >  2:298819/1‑300 (MQ=14)                                                                                                                                                                                                                                   aCGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTg                                             >  1:370663/1‑300 (MQ=255)                                                                                                                                                                                                                                   aCGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGAATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAAGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGCGTGTTTCGAAGAACTTGTTTATCGCTCATCGTCGCTg                                             >  2:24849/1‑300 (MQ=255)                                                                                                                                                                                                                                     gTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCg                                           >  1:794673/1‑300 (MQ=255)                                                                                                                                                                                                                                      ttATCATCCCTAGTGCCTGCGGT‑TAATTCCGCTACCTCTTCGATACCTTCA‑TTGCTG‑AAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGCGGCGGGGGTTCGACCCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCCGGTGAGCGCATTCAGCCTCTAGGCCACACCACCCCACTCCTCTTACGAGTACTTCGCAGAACTTGTTTATCGCTCATCGTCGCTGCg                                           >  2:797173/1‑300 (MQ=17)                                                                                                                                                                                                                                       tATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTg                                         >  2:750034/1‑300 (MQ=255)                                                                                                                                                                                                                                       gacagtccCTAGTGCCTGCGGT‑TAATTCCGCTACCTCTTCGATACCTTCA‑TTGCTG‑AAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGt                                          <  1:840488/295‑1 (MQ=21)                                                                                                                                                                                                                                            tccatGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCACGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCAACACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGgcgga                                    >  2:132116/5‑298 (MQ=255)                                                                                                                                                                                                                                            tccCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATNCAGCTGAGCTACGGATGCAAATGGCGNTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGca                                    >  2:594123/1‑300 (MQ=255)                                                                                                                                                                                                                                            tccCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGca                                    >  2:149865/1‑300 (MQ=255)                                                                                                                                                                                                                                            tccCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGca                                    >  1:246951/1‑300 (MQ=255)                                                                                                                                                                                                                                            tccCTAGTGCCTGCGGT‑TAATTCCGCTACCTCTTCGATACCTTCA‑TTGCTG‑AAGATNTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGNCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGtctgtc                                     >  2:821398/1‑295 (MQ=21)                                                                                                                                                                                                                                                 ggTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACatat                               >  2:286959/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gCGGT‑TATTACCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTAGCTGCGTGGCGCACATATTACTTTc                        >  1:552635/1‑300 (MQ=255)                                                                                                                                                                                                                                                               attaCCGCCACCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGAc                  >  1:518993/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        aCCTCTTCGATACCTTCA‑TTGCTG‑AAGATTGCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGt          <  2:864071/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aCCTCTTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGTc         >  1:720041/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          ctctTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGTCaa       >  2:691651/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tctTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCANCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTTAGACTTATAAGTCaaa      >  1:595316/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            ctTCTATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGCGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATTGTCGCTGCGTGTCGCATATATTACTTTCTGCGACTTATAAGTCAAAc     >  2:371120/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            ctTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGNCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGTCAAAc     >  1:632204/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            ctTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGTCAAAc     >  1:711976/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            ctTCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAACAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGCTTATCGCTCATCGTCGCTGCGTGGCGCATATATTACTTTCTGAGACTTATAAGTCAAAc     >  2:60093/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             ttCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGTCAAACa    >  2:291094/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             ttCCATACCTCAA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATACGTCAAACa    >  2:291104/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              tCCATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAAGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGTCAAACaa   >  2:342178/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               ccATACCTCCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGTCAAACAAt  >  1:593623/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                       ACTAAAGCCATATCAAGTAAGAGATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAA‑CTTACTTTACTG‑CAGATTTTTTGATACCGCTACTAAAGCCGTATCAAGTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCCTCCCTGGTGCCTGCGGT‑TATTACCGCCACCTCTTCCATACCTTCA‑TCGCTG‑AA‑AGTACGTAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCAAATGGCGGTGAGGCGGGGATTCGAACCCCGGATGCAGCTTTTGACCGCATACTCCCTTAGCAGGGGAGCGCCTTCAGCCTCTCGGCCACCTCACCACACGCCTCTTACGAGTGCTTCGAAGAACTTGTTTATCGCTCATCGTCGCTGCGTGGCGCACATATTACTTTCTGAGACTTATAAGTCAAACAAT  >  NC_000913/2818174‑2818740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑