Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,018,102	C→T	A257V (GCC→GTC)	rmuC →	putative recombination limiting protein RmuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,018,102	0	C	T	100.0%	338.9 / NA	97	A257V (GCC→GTC)	rmuC	putative recombination limiting protein RmuC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (52/45);  total (52/45)

TCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGTTGAAAAACAACATCATGCTGGTTAGCCCGACTACGCTGCTGGTGGCGCTGCGC  >  NC_000913/4017811‑4018391                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                  gttatcaacatgcgcagcaaaaagccgagcaattagctgaacgtgaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGAGCGAAAGGTACGAATCTGAAAACCAGGTCAGCATCGAAAATGACGCCCTCTCGCGGATGCAGCCGNATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACATCAAAATGa                                                                                                                                                                                                                                                                                           >  2:668983‑M2/175‑300 (MQ=255) gttatcaacatgcgcagcaaaaagccgagcaattagctgaacgtgaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataaagaagtgcgcagcctgcaaagtattaacacctatctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCCGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAACAGGTGGTGATCGACGTCAAAATGa                                                                                                                                                                                                                                                                                           >  2:281707‑M2/175‑300 (MQ=255)    atcaacatgcgcagcaaaaagccgagcaattagctgaacgtgaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGc                                                                                                                                                                                                                                                                                        <  1:752062‑M2/129‑1 (MQ=255)                 gcaaaaagccgagcaattagctgaacgtgaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctatctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGa                                                                                                                                                                                                                                                                           >  1:195358‑M2/159‑300 (MQ=255)                   aaaaagccgagcaattgactgaacttgaagagatggtcgcggaattaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgctagttactcaataactaaatgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCACCTATGAAc                                                                                                                                                                                                                                                                         <  2:309819‑M2/144‑1 (MQ=255)                   aaaaagccgagcaattagctgaacgttaatagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaantattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGTTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAAc                                                                                                                                                                                                                                                                         <  2:210763‑M2/144‑1 (MQ=255)                   aaaaagccgagcaattagctgaacgggaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCACTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAAc                                                                                                                                                                                                                                                                         <  2:441164‑M2/144‑1 (MQ=255)                   aaaaagccgaacaattagctgaacgtgaagagatgggcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgctagttacttaataacgaagtgtgcagcctgcaaagtattaacacctctctggaggccgGCCTGCGTGAAGGGTATGAATACGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCATGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAAc                                                                                                                                                                                                                                                                         <  2:131857‑M2/144‑1 (MQ=255)                           gtgtataagagacagcgtgaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCAAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTAtttt                                                                                                                                                                                                                                                                 <  1:325309‑M2/152‑1 (MQ=255)                             gcaattagctgaacgtgaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTaa                                                                                                                                                                                                                                                               >  2:580230‑M2/147‑300 (MQ=255)                              caattagctgaacgtgaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAAc                                                                                                                                                                                                                                                              >  1:899839‑M2/146‑300 (MQ=255)                               aattagctgaacgtgaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACg                                                                                                                                                                                                                                                             >  1:869123‑M2/145‑300 (MQ=255)                                attagctgaacgtgaagagatggtcgcggagttaagcgcggcaaaaaaacaaattaccaaaagcgagcattggcgtgcagagtgcgagttgctaaataacgaagtgcgcagcctgcaaagtattcacacctatatggaggacgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGACCGCTCGCGGATGCCGTAGGATGTCATAGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATTGACGTCACAGTGACACTGGTCGACTATGAACACTATTTTaacaa                                                                                                                                                                                                                                                            >  2:361090‑M2/144‑298 (MQ=255)                                   agatgaaagggaggggatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGa                                                                                                                                                                                                                                                         <  2:491613‑M2/160‑1 (MQ=255)                                          cgttaatagatggtcgcggagttaagcgctgcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAgacgac                                                                                                                                                                                                                                                  <  2:77327‑M2/167‑1 (MQ=255)                                          cgtgaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCAAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTcgcctatgaacgctattttctgtctcttatacac                                                                                                                                                                                                                                                  >  2:322963‑M2/134‑285 (MQ=255)                                             gaagagatggtcgcggagttacgcgcggcaaaacaacaaattacccaacgcgagccctggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctatggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAAGTGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTCTTAACGCTATTTTCACGCCGAAGACGACTac                                                                                                                                                                                                                                               >  2:258677‑M2/131‑300 (MQ=255)                                             gaagagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagnactggcgtgcagagtgcgagttactcaataacgaagtgcgcaacctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTac                                                                                                                                                                                                                                               <  2:673518‑M2/170‑1 (MQ=255)                                               agagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcantggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGANGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTacac                                                                                                                                                                                                                                             <  2:835448‑M2/172‑1 (MQ=255)                                               agagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATNACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATNAACGCTATTTTAACGCCGAAGACGACTacac                                                                                                                                                                                                                                             <  2:641647‑M2/172‑1 (MQ=255)                                                tagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACAcc                                                                                                                                                                                                                                            <  1:103493‑M2/173‑1 (MQ=255)                                                gagatggtcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACAcc                                                                                                                                                                                                                                            <  1:166063‑M2/173‑1 (MQ=255)                                                       tcgcggagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCNGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGaaa                                                                                                                                                                                                                                     >  1:661174‑M2/121‑300 (MQ=255)                                                            gagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCACAATGACGCCGGTCGACTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAgcgcg                                                                                                                                                                                                                                >  1:793859‑M2/116‑300 (MQ=255)                                                            gagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGACGAAGACGACTACACCCGCGAAAgcgcg                                                                                                                                                                                                                                >  2:430236‑M2/116‑300 (MQ=255)                                                            gagttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAAACCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAgcgcg                                                                                                                                                                                                                                >  2:157714‑M2/116‑300 (MQ=255)                                                              gttaagcgcggcaaaacaacaaattacccaangcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAANGGTATGAATATGAAACCCAGGTCAGCCTCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCt                                                                                                                                                                                                                              >  2:622743‑M2/114‑300 (MQ=255)                                                              gttaagcgcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCt                                                                                                                                                                                                                              >  1:693939‑M2/114‑300 (MQ=255)                                                                     gcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgTTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGaa                                                                                                                                                                                                                       <  2:420634‑M2/194‑1 (MQ=255)                                                                     gcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTTAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACTTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGaa                                                                                                                                                                                                                       <  2:422504‑M2/194‑1 (MQ=255)                                                                     gcggcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGaa                                                                                                                                                                                                                       <  1:411819‑M2/194‑1 (MQ=255)                                                                        gcaaaacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGNTGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGNCTACACCCGCGAAAGCGCGCTACAGGAACat                                                                                                                                                                                                                    <  2:641331‑M2/197‑1 (MQ=255)                                                                          aaaacaacaaattacccaaagcgagcactggcgtgcagagcgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGGGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACatat                                                                                                                                                                                                                  <  2:761312‑M2/199‑1 (MQ=255)                                                                            aacaacaaattacccaaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGAGGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATcg                                                                                                                                                                                                                <  2:195355‑M2/201‑1 (MQ=255)                                                                                          caaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAAc                                                                                                                                                                                                  <  1:283389‑M2/215‑1 (MQ=255)                                                                                           aaagcgagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAAcc                                                                                                                                                                                                 <  1:384200‑M2/216‑1 (MQ=255)                                                                                                gagcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATc                                                                                                                                                                                            >  1:252882‑M2/80‑300 (MQ=255)                                                                                                 agcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATcc                                                                                                                                                                                           <  2:502557‑M2/222‑1 (MQ=255)                                                                                                  gcactggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCg                                                                                                                                                                                          >  2:348700‑M2/78‑300 (MQ=255)                                                                                                     ctggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGttt                                                                                                                                                                                       >  1:783203‑M2/75‑300 (MQ=255)                                                                                                       ggcgtgtagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGc                                                                                                                                                                                     >  1:11108‑M2/73‑300 (MQ=255)                                                                                                       ggcgtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGc                                                                                                                                                                                     >  1:386971‑M2/73‑300 (MQ=255)                                                                                                          gtgcagagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacanctctctggaggccgGTCTGCGTGAAGGCTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTgg                                                                                                                                                                                  <  2:659786‑M2/231‑1 (MQ=255)                                                                                                               gagtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGc                                                                                                                                                                             >  1:542670‑M2/65‑300 (MQ=255)                                                                                                                 gtgcgagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAAACATATCCGTTTGCTGGGACGCaa                                                                                                                                                                           >  2:371127‑M2/63‑300 (MQ=255)                                                                                                                     gagttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGAt                                                                                                                                                                       >  1:35837‑M2/59‑300 (MQ=255)                                                                                                                        ttactcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCNGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGNTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTAt                                                                                                                                                                    >  2:700577‑M2/56‑300 (MQ=255)                                                                                                                        ttactcaataacgaagtgcgcagcctgcaaagtattaacacctatctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAGAGGTGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAACGCGCTACAGGAACATATCGCGTCGGTGTGTATCTATATCCGTTTGCTGGGACGCAAAGACTAt                                                                                                                                                                    >  2:368570‑M2/56‑300 (MQ=255)                                                                                                                            tcaataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAAc                                                                                                                                                                <  1:123231‑M2/249‑1 (MQ=255)                                                                                                                             caataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACa                                                                                                                                                               >  1:328472‑M2/51‑300 (MQ=255)                                                                                                                               ataacgaagtgtgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGc                                                                                                                                                             >  2:809627‑M2/49‑300 (MQ=255)                                                                                                                               ataacgaagtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGc                                                                                                                                                             >  1:444041‑M2/49‑300 (MQ=255)                                                                                                                                      agtgcgcagcctgcaaagtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCggg                                                                                                                                                      <  1:82818‑M2/259‑1 (MQ=255)                                                                                                                                               cctgcaaagtattaacacctctctgtaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATTTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGGGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAAc                                                                                                                                             <  1:437795‑M2/268‑1 (MQ=255)                                                                                                                                                    aaagtattaacacctctctggaggccgTTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTttgctgggacgcaaagattatcaacctgtctcttatacacatctcgcag                                                                                                                                        <  1:351468‑M2/273‑25 (MQ=255)                                                                                                                                                      agtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGAt                                                                                                                                      <  1:889428‑M2/275‑1 (MQ=255)                                                                                                                                                       gtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGAtt                                                                                                                                     >  1:126899‑M2/25‑300 (MQ=255)                                                                                                                                                       gtattaacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGAtt                                                                                                                                     >  2:805545‑M2/25‑300 (MQ=255)                                                                                                                                                         attaacacctctctggaggccgGTCTGCGGGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTTGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTAc                                                                                                                                   <  2:456240‑M2/278‑1 (MQ=255)                                                                                                                                                           taacacctctctggaggccgGTCTGCGTTAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGCTGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGt                                                                                                                                 <  2:535396‑M2/280‑1 (MQ=255)                                                                                                                                                           taacacctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCATGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGt                                                                                                                                 <  1:366447‑M2/280‑1 (MQ=255)                                                                                                                                                                cctctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGa                                                                                                                            >  1:18522‑M2/16‑300 (MQ=255)                                                                                                                                                                  tctctggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGNAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATg                                                                                                                          >  1:629112‑M2/14‑300 (MQ=255)                                                                                                                                                                  tctcgggaggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCTGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTTGGACGCAAAGATTATCAACAGCTGCCTGGGCTGCGAACTCTGGATTACGTGCTGATg                                                                                                                          <  2:148406‑M2/287‑1 (MQ=255)                                                                                                                                                                         aggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGTGCGCTAAAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTc                                                                                                                   >  2:65104‑M2/7‑300 (MQ=255)                                                                                                                                                                         aggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTc                                                                                                                   >  1:798801‑M2/7‑300 (MQ=255)                                                                                                                                                                          ggccgGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTcc                                                                                                                  <  1:812975‑M2/295‑1 (MQ=255)                                                                                                                                                                               gTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTg                                                                                                             <  2:797179/300‑1 (MQ=255)                                                                                                                                                                                   gCGTGAAGGGTATGAATACGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAAcc                                                                                                         >  1:664856/1‑300 (MQ=255)                                                                                                                                                                                       gAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCt                                                                                                     <  1:116766/300‑1 (MQ=255)                                                                                                                                                                                          gggTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACATCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCtttt                                                                                                  >  1:795645/1‑300 (MQ=255)                                                                                                                                                                                          gggTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCtttt                                                                                                  >  1:250417/1‑300 (MQ=255)                                                                                                                                                                                          gggTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCtttt                                                                                                  >  2:442342/1‑300 (MQ=255)                                                                                                                                                                                          gggTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGACTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGCCGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCtttt                                                                                                  >  2:178931/1‑300 (MQ=255)                                                                                                                                                                                           ggTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCttttt                                                                                                 >  1:666868/1‑300 (MQ=255)                                                                                                                                                                                            gTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGACTATGAACGCTATTTTAACGCCGACTACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCAGGNGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCATTGAACCCGCtttttt                                                                                                >  2:669294/1‑300 (MQ=255)                                                                                                                                                                                                         aCCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGAc                                                                                   >  1:340249/1‑300 (MQ=255)                                                                                                                                                                                                             aGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTANGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTTGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGcc                                                                               <  1:640911/300‑1 (MQ=255)                                                                                                                                                                                                              ggTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCa                                                                              >  1:122601/1‑300 (MQ=255)                                                                                                                                                                                                                   gCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGcagg                                                                         >  1:522022/1‑297 (MQ=255)                                                                                                                                                                                                                   gCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCgg                                                                         >  1:701011/1‑300 (MQ=255)                                                                                                                                                                                                                   gCATCGAAAATAACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAACTGCCGGGGCTGCGAACTCTGGATTACGTGNTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGccag                                                                         >  2:674339/1‑298 (MQ=255)                                                                                                                                                                                                                           aaTGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATc                                                                 <  1:826885/300‑1 (MQ=255)                                                                                                                                                                                                                             tGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCAc                                                               <  2:516745/300‑1 (MQ=255)                                                                                                                                                                                                                                      cTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGtt                                                      >  1:722526/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACATGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTTGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGTTGAAAAACAAcat                                          <  2:188023/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGAACGCCAGCCGGAGCTGATCACCGAAGCGTTGAAAAACAACATCATg                                      >  1:210085/1‑300 (MQ=255)                                                                                                                                                                                                                                                       aTGTCATCGTGCGCCTGCCGCAGGGAAAAGATTTGGTGATCGACGTTAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCTTCGGTGCTTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATATTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGTTTAAAAACAACATCATGc                                     <  2:173374/300‑1 (MQ=255)                                                                                                                                                                                                                                                       aTGTAATCGTGCGCCTGCCGCAGAGAAAAGATGTGGTTATCGACGTCACCCTGACGCTAGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAGGCGCGCTACAGGAACATATCGCGTCGGTGTGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCACCAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGCTGTCTATTCCCGTTGAACCCACTTTTTTACTGGCGATTGACCGCCAGCCGGAGCTGATCAACGAAGCGTTGAAAAACAACATCATga                                     >  2:464511/1‑299 (MQ=255)                                                                                                                                                                                                                                                        tGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACTTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGTTGAAAAACAACATCATGCt                                    <  2:549525/300‑1 (MQ=255)                                                                                                                                                                                                                                                         gTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGTTGAAAAACAAAGTCATGCTg                                   >  2:98165/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTAATGGCGCTTGACCGCCAGCCGGAGCTGATCAACGAAGCGTTGAAAAACAACATCATGCTg                                   >  2:95129/1‑300 (MQ=255)                                                                                                                                                                                                                                                          tCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGTTGAAAAACAACATCATGCTgg                                  <  1:686068/300‑1 (MQ=255)                                                                                                                                                                                                                                                            aTCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGTTGAAAAACAACATCATGCTGGtt                                <  1:418122/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 gcgcCTGCCGCAGGGAAAAGATGTGGTGATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGTTGAAAAACAACATCATGCTGGTTAGccc                           <  1:848913/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gtgATCGACGTCAAAATGACGCTGGTCGCCTATGAACGCTATTTCAACGCCGAAGGCGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATTACCGAAGCGTTGAAAAACAACATCATGCTGGTTAGCCCGACTACGCTGCTAGTGGCGCTgcgc  >  2:38606/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          gtgATCGACGTCAAAATGACGCTGGTCGCCGATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCTAAGCGTTGAAAAACAACATCATGCTGGTTAGCCCGACTACGCTGCTGGTGGCGCTgcgc  <  1:186147/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                  TCGGTAAAGAAGCACAAGAACGCCATACCCTGACCCACGAAATTCGCAATCTCCAGCAACTCAACGCGCAAATGGCCCAGGAAGCGATCAACCTGACGCGCGCGCTGAAAGGCGACAATAAAACCCAGGGCAACTGGGGCGAGGTAGTATTGACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCGAACTCTGGATTACGTGCTGATGTTTATTCCCGTTGAACCCGCTTTTTTACTGGCGCTTGACCGCCAGCCGGAGCTGATCACCGAAGCGTTGAAAAACAACATCATGCTGGTTAGCCCGACTACGCTGCTGGTGGCGCTGCGC  >  NC_000913/4017811‑4018391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑