Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,522,583	C→T	Q161Q (CAG→CAA)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,522,583	0	C	T	100.0%	204.8 / NA	59	Q161Q (CAG→CAA)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/41);  total (18/41)

AGCCTTCCATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGA  >  NC_000913/1522289‑1522867                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                             aGCCTTCCATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGttgttg                                                                                                                                                                                                                                                                                         >  1:882870/1‑300 (MQ=255)  gCCTTCCATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTtgttgt                                                                                                                                                                                                                                                                                        >  1:747730/1‑300 (MQ=255)   ccTTCCATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTTAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTc                                                                                                                                                                                                                                                                                       <  2:484906/300‑1 (MQ=255)    cTTCCATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTcc                                                                                                                                                                                                                                                                                      <  2:512068/300‑1 (MQ=255)    cTTCCATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATNGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCNGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTcc                                                                                                                                                                                                                                                                                      >  2:830886/1‑300 (MQ=255)       ccATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGt                                                                                                                                                                                                                                                                                   >  1:754147/1‑300 (MQ=255)             tgcgttGGTTAACCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGggt                                                                                                                                                                                                                                                                             <  2:680191/296‑1 (MQ=255)                   ggTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTACCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGAc                                                                                                                                                                                                                                                                       >  1:787557/1‑300 (MQ=255)                       aGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGAcattc                                                                                                                                                                                                                                                                   <  1:59239/300‑1 (MQ=255)                           cACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGNCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAANTAATGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTa                                                                                                                                                                                                                                                               >  2:830981/1‑300 (MQ=255)                           cACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTa                                                                                                                                                                                                                                                               >  1:367868/1‑300 (MQ=255)                             ccTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTAcc                                                                                                                                                                                                                                                             >  1:820253/1‑300 (MQ=255)                                    cATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCTGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAg                                                                                                                                                                                                                                                      <  1:275702/300‑1 (MQ=255)                                       cTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGNCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCNCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGAcg                                                                                                                                                                                                                                                   <  2:705430/300‑1 (MQ=255)                                       cTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGAcg                                                                                                                                                                                                                                                   <  1:206467/300‑1 (MQ=255)                                                  tCCACACTGTTTAAAATTAGACTTAAGTTGCTGGCTTCATCAATACGTACGCCCAGTTTGGCATTGGCTAGATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGGGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATAc                                                                                                                                                                                                                                        <  2:336853/300‑1 (MQ=255)                                                  tCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGTGTACGCCCAGTTTTGCATTGGCTAAATTTTTCTGTGCGCCACTGTGGTTACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAAGCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTGGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATAc                                                                                                                                                                                                                                        <  2:304940/300‑1 (MQ=255)                                                  tCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTTAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATAc                                                                                                                                                                                                                                        <  1:682426/300‑1 (MQ=255)                                                  tCCACACTATTGAAAATCAAACTTAATTTGCTGGCTTCATCAATACGGACGCCCATGTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGTTTTATACGGTGTAATCGACATCGCCAGGCTGGGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATAc                                                                                                                                                                                                                                        <  2:145871/300‑1 (MQ=255)                                                  tCCACACGCTTGAAAATCAGACTTAATTGTCTAGCTCCATCAATGCGTACGCCCAGTTTTGCATTGGCTAAATTTTTCTGTGCGCCACTGTGGTCATGATAGCCGTGGGTTGTAAAACGCGTGTTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGGTGCGCCCGTTGCTTTCAGCCCATGGCGCCAGCTGCCAAAACTGCCTTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATAc                                                                                                                                                                                                                                        <  2:95909/300‑1 (MQ=255)                                                  tCCACACGATTGAAAATCAGACTTGATTTGTTGGCTTCATCGATGCGTACGCCCAGTTTGGCATTTGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTTAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTTCCGTAGTAACTACTGGCTTCAATGGTGGGTGGTTGTTGTCCGGTCTGGGTGTTGACATTCATTACCCCACCAGACGCGTTGCCATAc                                                                                                                                                                                                                                        <  2:250459/300‑1 (MQ=255)                                                    cacaCTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTTAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAg                                                                                                                                                                                                                                      <  1:513152/300‑1 (MQ=255)                                                       acTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTNGCATTGGCTAAATTTTTCTGTTCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGNTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGc                                                                                                                                                                                                                                   <  2:815546/300‑1 (MQ=255)                                                        cTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCa                                                                                                                                                                                                                                  >  1:434094/1‑300 (MQ=255)                                                            tGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGAACATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAgaga                                                                                                                                                                                                                              <  1:781839/300‑1 (MQ=255)                                                                  tCAGACTTAATTTGCTTGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGc                                                                                                                                                                                                                        <  1:104911/300‑1 (MQ=255)                                                                         tAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACgcag                                                                                                                                                                                                                 <  1:673212/300‑1 (MQ=255)                                                                               gCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTc                                                                                                                                                                                                           <  1:15423/300‑1 (MQ=255)                                                                                  ggCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCcac                                                                                                                                                                                                        >  1:680189/1‑300 (MQ=255)                                                                                           aaTGCGTATTCCCAGTTTGGGATTGGCTAAATTTTTCTGTGCGCCATTGTGTTCACGGTGGCCGTGGTTCGTGAAACGCTTGTTTGAGACGGTGTAATCGACGTCGCCATGCTGTGTGCCGGCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCGGCCAAAACTGCCGTAGTAACTACTTGCTTCAATTGTTGGTGTTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGcac                                                                                                                                                                                               <  2:190234/300‑1 (MQ=255)                                                                                           aaTGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGcac                                                                                                                                                                                               <  1:556309/300‑1 (MQ=255)                                                                                                         gTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGa                                                                                                                                                                                 >  2:38049/1‑300 (MQ=255)                                                                                                         gTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTACACATTTTGCACACTGCTTAAATCGa                                                                                                                                                                                 >  1:330227/1‑300 (MQ=255)                                                                                                                  tGGCTAAATTTGTCTGTACGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGGGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATg                                                                                                                                                                        <  2:14164/300‑1 (MQ=255)                                                                                                                              tCTGTGCGCCACTATAGTCACGATAGCCGGGGGTCGTAAAACGCGTGGGTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCTTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAGTGGTTGGTGTTTGTTGNCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTTCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTNCTTAAATCGATGTTGGATGTttgcccttgcc                                                                                                                                                            <  2:626753/300‑1 (MQ=255)                                                                                                                                   gcgcCACTATGGTCACGATAGCCGTGGGTCGTACAACGCGTGGTTGAGACGGTGTAATCGAAATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTCCCCCACCAGACGCGTTGCCATAAAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTACATCGATGTTGGATGTTTGCCCTTGCCCGTCg                                                                                                                                                       >  1:135304/1‑300 (MQ=255)                                                                                                                                      ccACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGc                                                                                                                                                    >  2:123298/1‑300 (MQ=255)                                                                                                                                           aTGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCAtggt                                                                                                                                               <  1:130438/300‑1 (MQ=255)                                                                                                                                                      aGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTNTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATAc                                                                                                                                    <  2:666868/300‑1 (MQ=255)                                                                                                                                                      aGCCGTGGGTCGTAAAACGCGTGGTCGAGACGGTGTAATCGACATCGCCAGGCTGTGTACCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCACCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGTTGGTTGTTGTCCGGTCTGGGTGTTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATAc                                                                                                                                    <  2:3656/300‑1 (MQ=255)                                                                                                                                                                cGTAAAACGCGGGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCTTAGNAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATa                                                                                                                          <  1:596131/300‑1 (MQ=255)                                                                                                                                                                 gTAAAACGCATGGTTTAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATAc                                                                                                                         <  1:216423/300‑1 (MQ=255)                                                                                                                                                                    acatgcgTGGTTTAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCGGCCCATAGCGCCAGCTGCCAAAACTGCCGTGGTAATTACTGGCTTCAATGGTGGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAgg                                                                                                                      <  2:515532/296‑1 (MQ=255)                                                                                                                                                                           tgttttagaCGGGGTGATCGACAGCTCCACGCTGTGTACTGCCTCCCGTTGCGCCCGTTGCTTTCATCCCATAGCGCCAGCTGCCAAAACTGCCGTAGGAACTACTGGCTGCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGTTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGTGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaatac                                                                                                               <  2:251735/294‑1 (MQ=255)                                                                                                                                                                           tGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaatac                                                                                                               <  1:421050/300‑1 (MQ=255)                                                                                                                                                                           tGGTTGAGACAGTGTAATCGACATCGCCATGCTGTGTGCCGTCTCCCGTTGCACCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaatac                                                                                                               <  2:473172/300‑1 (MQ=255)                                                                                                                                                                            ggTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTCAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATATCGTCCACATACAGgcgaatacc                                                                                                              >  2:464138/1‑300 (MQ=255)                                                                                                                                                                                    cGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGgcgaataccgcgaatac                                                                                                      <  1:103717/300‑1 (MQ=255)                                                                                                                                                                                             cGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGNTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTgg                                                                                             <  1:630534/300‑1 (MQ=255)                                                                                                                                                                                                  tCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACTGAGAATACCGTAAGTGGAGCgg                                                                                        >  2:318624/1‑300 (MQ=255)                                                                                                                                                                                                            gtgtgCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGTTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATc                                                                              <  1:758582/300‑1 (MQ=255)                                                                                                                                                                                                                     ctcCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCg                                                                     >  1:209537/1‑300 (MQ=255)                                                                                                                                                                                                                                 ccGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGTTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAAt                                                         <  1:731514/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gcgcCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGTTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATAAAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCg                                      <  1:228024/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gcgcCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGNCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCg                                      <  1:643444/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gcgcCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCg                                      <  1:452003/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     ggCTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAg     >  1:565041/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       cTTCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATNCCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTg   >  1:661876/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        ttCACTGGTTTGTGGTTTTTTTCCGGTCTGTGTGGTGACATTCATTACCCCACTAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTTGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCATTGa  <  2:316180/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        ttCAATGGTTGGTGGTTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGa  <  1:832948/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                             AGCCTTCCATTCTGCTTTGGTTAGCCCACCTGGGTCATCTGCTTTGATATCCACACTATTGAAAATCAGACTTAATTTGCTGGCTTCATCAATGCGTACGCCCAGTTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAGCACTTCCACATTTTGCACACTGCTTAAATCGATGTTGGATGTTTGCCCTTGCCCGTCGGGCATGGTGGCGGGAATACCGTCCACATACAGGCGAATACCGCGAATACCGTAAGTGGAGCGGGAGCCAAATCCGCGAATCGACAGCTGTAAATCTTGCGCATAGTTCTGCCGGTTTTGTACCTGCAAACCAGGCACGCCGGTCAGTGA  >  NC_000913/1522289‑1522867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑