Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,570,269	G→A	P23P (CCC→CCT)	eutJ ←	putative ethanolamine utilization chaperonin EutJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,570,269	0	G	A	100.0%	211.1 / NA	60	P23P (CCC→CCT)	eutJ	putative ethanolamine utilization chaperonin EutJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/34);  total (26/34)

GCTCACTTCCAGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCA  >  NC_000913/2569975‑2570532                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                        gCTCACTTCCAGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGTGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACAGCCACTGGCCGAATGTCGCGGTCGACAACCCTCGACACCACATTGCAGGTGCCCAGATCCACCACACGCCAAAGCGAAGATCCCGTCGCGGCAGGCGt                                                                                                                                                                                                                                                                    >  2:72795/1‑300 (MQ=255) gCTCACTTCCAGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGt                                                                                                                                                                                                                                                                    >  1:250741/1‑300 (MQ=255)       ttCCATTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCATACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGtt                                                                                                                                                                                                                                                              <  2:262913/300‑1 (MQ=255)         ccattCCGGCAGATTCCAGCACGTTGATGGAAATACGCTGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGTTTac                                                                                                                                                                                                                                                            <  2:487011/296‑1 (MQ=255)           aatCCGGCAGATTCCAGCACGGTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCAAACCACATCGGCCCAGTCTAGGCATACCACCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTacac                                                                                                                                                                                                                                                          <  2:235371/298‑1 (MQ=255)           aGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGANATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTacac                                                                                                                                                                                                                                                          <  1:635839/300‑1 (MQ=255)           aGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTacac                                                                                                                                                                                                                                                          <  1:364233/300‑1 (MQ=255)           aGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTacac                                                                                                                                                                                                                                                          <  2:388092/300‑1 (MQ=255)             tCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAg                                                                                                                                                                                                                                                        <  1:816579/300‑1 (MQ=255)                ggCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGTATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGt                                                                                                                                                                                                                                                     <  2:539430/300‑1 (MQ=255)                ggCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGCGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGt                                                                                                                                                                                                                                                     >  2:860003/1‑300 (MQ=255)                ggCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGt                                                                                                                                                                                                                                                     <  1:301505/300‑1 (MQ=255)                   aGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTgc                                                                                                                                                                                                                                                  <  1:264759/300‑1 (MQ=255)                     aTTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTgccg                                                                                                                                                                                                                                                <  1:385996/300‑1 (MQ=255)                        ccAGCACGTTGATGGAAAGACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGccgccg                                                                                                                                                                                                                                             <  2:285545/300‑1 (MQ=255)                                      gAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGACCCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATTCACCCACAGCCACAGCGGAGATTCCGTAGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTggg                                                                                                                                                                                                                               >  2:285987/1‑300 (MQ=255)                                       aaaTACGCTGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCTAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTgggg                                                                                                                                                                                                                              <  2:154325/300‑1 (MQ=255)                                             gcgGGTCCGTACCTTGAGGAAATGAAGTCGCCGCATGGCTAAAACTACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCTAAGAAATCCCAGACGATGCCGTCGCGAANCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGcc                                                                                                                                                                                                                        <  2:220840/300‑1 (MQ=255)                                                ggTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGNCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGNGCCAtt                                                                                                                                                                                                                     >  2:726563/1‑300 (MQ=255)                                                    cGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTtc                                                                                                                                                                                                                 <  2:685204/300‑1 (MQ=255)                                                     gTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTtcg                                                                                                                                                                                                                >  1:505371/1‑300 (MQ=255)                                                      tACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTtcgt                                                                                                                                                                                                               <  1:677886/300‑1 (MQ=255)                                                                       gTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCANATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTAc                                                                                                                                                                                              <  1:595091/300‑1 (MQ=255)                                                                       gTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTAc                                                                                                                                                                                              <  1:287514/300‑1 (MQ=255)                                                                           ccgcATGGCTAAAACGACGACCGAATTGCGGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCTAGGCATACCGCCACTGGCTGACCGTCGCGNTCGACAACCATCGACACCACATCGCANGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCATCTGTTTACACAGCGTTGCCGCCGGTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCt                                                                                                                                                                                          <  2:614274/300‑1 (MQ=255)                                                                                tGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAAc                                                                                                                                                                                     <  1:69658/300‑1 (MQ=255)                                                                                tGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAAc                                                                                                                                                                                     <  2:398842/300‑1 (MQ=255)                                                                                tGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTAAAAc                                                                                                                                                                                     <  1:434621/300‑1 (MQ=255)                                                                                 ggCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACCCAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTCAACa                                                                                                                                                                                    >  2:801973/1‑300 (MQ=255)                                                                                  gCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACaa                                                                                                                                                                                   <  2:349617/300‑1 (MQ=255)                                                                                            cgacCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCAAATCGCAGGTGCCCAGNTCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGAAGGCGTCTGGTTAAACAGCGTTGCCGCCGTTTGTCGACGTGGGGTGAGCCACTGTTCGTCGTGTGCCATCTGTTACTCATTAAAAAGTGCGaagcga                                                                                                                                                                         >  2:219108/1‑296 (MQ=255)                                                                                                        tgctCGAGCGTGTCTAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCATACGATGCCGTCGCGAACCACATCGGCCCAGTCTAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCNACACCACATCGCAGGTGCCCAAATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTGTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACGATGCGAAACGCATCGATTAATac                                                                                                                                                             <  2:228299/300‑1 (MQ=255)                                                                                                        tgctCGAGCGTGTCGAGATGTCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATac                                                                                                                                                             <  2:855296/300‑1 (MQ=255)                                                                                                          ctCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATacac                                                                                                                                                           <  1:488687/300‑1 (MQ=255)                                                                                                          ctCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATacac                                                                                                                                                           <  2:365756/300‑1 (MQ=255)                                                                                                                  tgtCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGaagc                                                                                                                                                   >  1:29088/1‑297 (MQ=255)                                                                                                                            gCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGNCACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCNTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACaa                                                                                                                                         <  2:713754/300‑1 (MQ=255)                                                                                                                            gCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACaa                                                                                                                                         >  1:897992/1‑300 (MQ=255)                                                                                                                                     aaTGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTACTTAAACAATGCGAAACGCATCGACTAATACACAAAGACGCAGACGGACAAATGTAcgtg                                                                                                                                >  2:438653/1‑298 (MQ=255)                                                                                                                                                  ccGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTAcccc                                                                                                                   <  1:44154/300‑1 (MQ=255)                                                                                                                                                       gaaATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTGACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCGTCAc                                                                                                              <  2:29028/300‑1 (MQ=255)                                                                                                                                                          aTCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCgg                                                                                                           <  2:802355/300‑1 (MQ=255)                                                                                                                                                                      gCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGa                                                                                               <  1:182229/300‑1 (MQ=255)                                                                                                                                                                               aaCCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCAtgg                                                                                      >  1:449519/1‑300 (MQ=255)                                                                                                                                                                                 ccACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCAtggtg                                                                                    >  1:744565/1‑300 (MQ=255)                                                                                                                                                                                    caTCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTc                                                                                 >  1:46887/1‑300 (MQ=255)                                                                                                                                                                                             aGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTcc                                                                        >  1:787660/1‑300 (MQ=255)                                                                                                                                                                                              gTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCCCCTTTTTGCGTGGTGCTGGTCATGGTGGTCCAGCCTTccc                                                                       >  2:518263/1‑300 (MQ=255)                                                                                                                                                                                              gTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGTGTGGTGATGGTCATGGTGGTCCAGCCTTccc                                                                       >  2:11002/1‑300 (MQ=255)                                                                                                                                                                                                  aGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGcc                                                                   <  1:875279/300‑1 (MQ=255)                                                                                                                                                                                                           gCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGATTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGcccagccccag                                                          >  2:812206/1‑300 (MQ=255)                                                                                                                                                                                                           gCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTAACCGGTTGGCGTGGTGATGGTAATGGTGGTCCAACCTTCCCCGcctagccccag                                                          >  2:450036/1‑300 (MQ=255)                                                                                                                                                                                                                 ggCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGc                                                    >  1:35726/1‑300 (MQ=255)                                                                                                                                                                                                                                  gACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCATTTGCAGACGTGGGGNGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAANACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCt                                   <  2:713336/300‑1 (MQ=255)                                                                                                                                                                                                                                                  acaTCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGt                   >  2:498600/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCAt        >  1:442922/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCAt        >  2:876965/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCACGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGcc   >  2:678661/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGAAGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCATTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGca   >  1:429162/1‑299 (MQ=255)                                                                                                                                                                                                                                                                   aTCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCAGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCa  >  2:87096/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                        GCTCACTTCCAGTCCGGCAGATTCCAGCACGTTGATGGAAATACGCGGGTCCGTACCTGGAGGAAATGAAGTCGCCGCATGGCTAAAACGACGACCGAATTGCTGCTCGAGCGTGTCGAGATGGCGACGAACAATGGTGACAGCGCCGAAGAAATCCCAGACGATGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCGCTGGTTACCCCTTCACCGGTTGGCGTGGTGATGGTCATGGTGGTCCAGCCTTCCCCGCCCAGCCCCAGCCCGGCAATGCACGGTCCGTTCTTAACGAAAATGCTGGTATCAATAGCATTCGCCA  >  NC_000913/2569975‑2570532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑