Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,027,332	T→A	E229V (GAA→GTA)	dgcQ ←	diguanylate cyclase DgcQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,027,332	0	T	A	97.8%	289.9 / NA	89	E229V (GAA→GTA)	dgcQ	diguanylate cyclase DgcQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (54/33);  minor base C (2/0);  total (56/33)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATC  >  NC_000913/2027042‑2027627                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                        tGTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCg                                                                                                                                                                                                                                                                                                <  1:116249/300‑1 (MQ=255)  gTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTANTGTCATAGAGCTGATACTCACCATCGNGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCGGTATTGCTGGCGTGTACCGGTTGCGa                                                                                                                                                                                                                                                                                               >  2:604883/1‑300 (MQ=255)  gTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTCCCGGTTGCGa                                                                                                                                                                                                                                                                                               >  1:498171/1‑300 (MQ=255)  gTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACTGGTTGCGa                                                                                                                                                                                                                                                                                               >  2:151326/1‑300 (MQ=255)  gTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGa                                                                                                                                                                                                                                                                                               >  2:537900/1‑300 (MQ=255)  gTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGa                                                                                                                                                                                                                                                                                               >  1:1408/1‑300 (MQ=255)  gTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAAACTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGa                                                                                                                                                                                                                                                                                               >  1:490807/1‑300 (MQ=255)    ccATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAg                                                                                                                                                                                                                                                                                             <  1:78275/300‑1 (MQ=255)    ccATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGGTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAg                                                                                                                                                                                                                                                                                             <  1:78546/300‑1 (MQ=255)       tACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGa                                                                                                                                                                                                                                                                                          >  2:251092/1‑300 (MQ=255)           aaTGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGAATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTCAAAATTGCTGCATAGTCCGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTTTACCGGAAAACTGACGGTAACCTGCGGTTCAGTATTGCTGTCGTGTACCGGTTGCGAAGTGAACCa                                                                                                                                                                                                                                                                                      >  2:106689/1‑300 (MQ=255)               ccgccCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGt                                                                                                                                                                                                                                                                                  <  1:20849/300‑1 (MQ=255)                          gtCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTg                                                                                                                                                                                                                                                                       >  1:702872/1‑300 (MQ=255)                           tCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGa                                                                                                                                                                                                                                                                      <  1:139310/300‑1 (MQ=255)                             aTGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACg                                                                                                                                                                                                                                                                    >  1:876391/1‑300 (MQ=255)                               gTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGAt                                                                                                                                                                                                                                                                  >  1:528825/1‑300 (MQ=255)                                 tCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGAttt                                                                                                                                                                                                                                                                >  1:709357/1‑300 (MQ=255)                                      tGCCTGCGCCAGCAAGGCAAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGGTTTTCTCg                                                                                                                                                                                                                                                           >  2:335483/1‑300 (MQ=255)                                                          gTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATaaaac                                                                                                                                                                                                                                       >  1:120220/1‑298 (MQ=255)                                                          gTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATaaaac                                                                                                                                                                                                                                       >  2:573826/1‑298 (MQ=255)                                                          gTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAAcc                                                                                                                                                                                                                                       >  1:175211/1‑300 (MQ=255)                                                            tCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAg                                                                                                                                                                                                                                     <  1:463310/300‑1 (MQ=255)                                                               cGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGt                                                                                                                                                                                                                                  <  1:663793/300‑1 (MQ=255)                                                               cGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTNTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGt                                                                                                                                                                                                                                  <  1:628214/300‑1 (MQ=255)                                                                   gATCAAAAATATTCCATGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGNTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTAGTTGTGAATGGCCAATAAACCAAGGTTggg                                                                                                                                                                                                                              >  2:225117/1‑300 (MQ=255)                                                                             atTCCCTGTTGGATGGTCAGGAGTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGcc                                                                                                                                                                                                                    <  2:474602/300‑1 (MQ=255)                                                                                           ggTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTg                                                                                                                                                                                                      >  1:545940/1‑300 (MQ=255)                                                                                             tCAGGATTGTAAGAGGTCAAAAATCTCAGCTTGCTGTCATAGAGCTGATACTCACCAGCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGa                                                                                                                                                                                                    <  2:259118/300‑1 (MQ=255)                                                                                                ggATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGCACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGagca                                                                                                                                                                                                 >  1:570460/1‑297 (MQ=255)                                                                                                 gATTGGAAGAGGTCAAAAATCTCAGCTTACTNTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACNCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACAt                                                                                                                                                                                                >  2:627753/1‑300 (MQ=255)                                                                                                 gATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACAt                                                                                                                                                                                                <  1:449939/300‑1 (MQ=255)                                                                                                                 aaaTCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGt                                                                                                                                                                                <  1:254093/300‑1 (MQ=255)                                                                                                                         gCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTAGTTGTGAATGGCAAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACCTTGCGAGTAAACAAGGTCGGCTGCg                                                                                                                                                                        >  2:453229/1‑300 (MQ=255)                                                                                                                                gTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATCAAACAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAAc                                                                                                                                                                 >  1:322736/1‑300 (MQ=255)                                                                                                                                gTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTAACGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAAc                                                                                                                                                                 >  1:312149/1‑300 (MQ=255)                                                                                                                                       agCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAAt                                                                                                                                                          >  1:884377/1‑300 (MQ=255)                                                                                                                                       agCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTAGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAAt                                                                                                                                                          >  1:180424/1‑300 (MQ=255)                                                                                                                                           gATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCgg                                                                                                                                                      >  2:698434/1‑300 (MQ=255)                                                                                                                                           gATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTAATGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCAGCTGCGTCGAAACGTAAAATCCgg                                                                                                                                                      >  2:351386/1‑300 (MQ=255)                                                                                                                                                      tCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGaca                                                                                                                                           <  2:840083/300‑1 (MQ=255)                                                                                                                                                          ggTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGacataca                                                                                                                                       >  1:137571/1‑300 (MQ=255)                                                                                                                                                           gTTTTTATCGATTGCTGTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTATTTACTACTGTCTACCTGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAAGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCTAGacatacat                                                                                                                                      <  2:23671/300‑1 (MQ=255)                                                                                                                                                           gTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGacatacat                                                                                                                                      <  1:724876/300‑1 (MQ=255)                                                                                                                                                             ttttATCGATGGCGTTTCTGAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCg                                                                                                                                    <  2:270788/300‑1 (MQ=255)                                                                                                                                                                              cTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTNCGAAGTGAACCAGCGTACCGCGCGGTNACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATcgaa                                                                                                                   >  2:613989/1‑299 (MQ=255)                                                                                                                                                                                  aaaaTTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACgagg                                                                                                               <  1:151793/300‑1 (MQ=255)                                                                                                                                                                                    aaTTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACgaggag                                                                                                             <  1:290949/300‑1 (MQ=255)                                                                                                                                                                                      ttGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAAACAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGGACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCACGTTCAAACATCGACGAGGAGtt                                                                                                           >  2:331514/1‑300 (MQ=255)                                                                                                                                                                                      ttGCTGCATAGTACGCACGGGAATACTCATCCACAGCACGCCATACAAGTAGTTATTAATGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCCATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAAAAAGGTCGGCTGCGTCGAAACATACACTCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGtt                                                                                                           >  2:331738/1‑300 (MQ=255)                                                                                                                                                                                           gcATAGTACGCACGGGAATACTCATCCCCAGCCCGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTgcg                                                                                                      >  1:852568/1‑300 (MQ=255)                                                                                                                                                                                           gcATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTgcg                                                                                                      >  1:807052/1‑300 (MQ=255)                                                                                                                                                                                            cATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTGCTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACTAGGAGTTGTgcgc                                                                                                     <  2:346708/300‑1 (MQ=255)                                                                                                                                                                                            cATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTgcgc                                                                                                     <  1:223457/300‑1 (MQ=255)                                                                                                                                                                                                   cGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCgc                                                                                              >  1:108689/1‑300 (MQ=255)                                                                                                                                                                                                    gCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCgca                                                                                             >  1:571001/1‑300 (MQ=255)                                                                                                                                                                                                         ggAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGCACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTCCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAACCCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACACACATCGCCTGTTCAAACATCGACGAGGAGTTGTGCGCTAGTAGCAGCAAg                                                                                        >  1:462058/1‑300 (MQ=255)                                                                                                                                                                                                         ggAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAACATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAg                                                                                        >  1:455032/1‑300 (MQ=255)                                                                                                                                                                                                         ggAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGTTAGTCGCAGCAAg                                                                                        >  1:145422/1‑300 (MQ=255)                                                                                                                                                                                                         ggAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAg                                                                                        >  1:762976/1‑300 (MQ=255)                                                                                                                                                                                                              aCTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAAcc                                                                                   <  1:193995/300‑1 (MQ=255)                                                                                                                                                                                                                            cGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCCGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAAACATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAg                                                                     >  2:45187/1‑300 (MQ=255)                                                                                                                                                                                                                            cGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAANGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAg                                                                     >  1:616911/1‑300 (MQ=255)                                                                                                                                                                                                                            cGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAg                                                                     >  1:426547/1‑300 (MQ=255)                                                                                                                                                                                                                              ccATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCCGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCg                                                                   >  1:697239/1‑300 (MQ=255)                                                                                                                                                                                                                                 tACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGNGTCGAAACGTCAAATCCGGCACGCGAGACATACATCGACTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTa                                                                >  2:787758/1‑300 (MQ=255)                                                                                                                                                                                                                                 tACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCATGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTa                                                                >  2:323205/1‑300 (MQ=255)                                                                                                                                                                                                                                  aCCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAACATCCGGCACGCGAGACATACATCGCCTGTTTAACCATCNACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAAATTCCAGTGCAGCGGTaa                                                               >  2:669857/1‑300 (MQ=255)                                                                                                                                                                                                                                         gtTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATGGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCAtt                                                        <  1:424567/300‑1 (MQ=255)                                                                                                                                                                                                                                          ttattaCTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACACAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCTTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACTATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGcggtggtgcgttcattg                                                       >  2:268763/1‑284 (MQ=255)                                                                                                                                                                                                                                             ttaCTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCCACCCCCGACGAAGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCCGTGCAGCGGTAATTTCGTTGTCg                                                    >  2:48876/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gTCTACCTGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAACCCAGGGTTGGGTGACATAGCCGTAATAACGCGTTNGAACATTGCGCGTAAACAAGGTCGGCNGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCt                                               <  2:596113/300‑1 (MQ=255)                                                                                                                                                                                                                                                  gTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCTAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTAGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCt                                               >  1:478340/1‑300 (MQ=255)                                                                                                                                                                                                                                                         ggAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCAttt                                        >  1:824415/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aCGGTAACCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGAc                               >  1:219137/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       aaCCTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGgag                          <  1:512092/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          cTGCGGTTCAGTATTGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACGTACATCGCCTGTTTAAAAATCGAAGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTTGAGGCTTTCATTTTCGCGAGACAGgagact                       >  2:299305/1‑298 (MQ=255)                                                                                                                                                                                                                                                                            gCGGTTCAGTACGGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCGCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCTAGACAGGAGAtgcc                     <  1:290657/300‑4 (MQ=255)                                                                                                                                                                                                                                                                                       ttGCTGGCGTGTACCGTTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACt          <  1:684446/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ttGCTGGCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGNTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACt          <  1:598669/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ggCGTGTACCGTTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGACTTTCTCGTTGTGAATGGCCAATAAACCAGGTTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCTAGACAGGAGATTCCCCTCGCTGACTAATGc     <  2:431877/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ggCGTGTACCGGTTGCGAAGTGAACCAGCGTNCCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACNAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGc     >  2:625076/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            ggCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAGTTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCACTCGCTGATTAATGc     >  2:46324/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            ggCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGc     <  2:491586/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ggCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGc     <  1:532623/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ggCGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAAACAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCCGCGTCGAAACGTCAAATCCGGCACGCGAGACATACATCACCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCCGCACGTAACCAACTTCCAGTGCAGCGGTAACTTNATTGTCGAGGATTTCGTTCCCGCGAGACAGGCGACTccccccaccgcctaatgc     >  2:662918/1‑286 (MQ=255)                                                                                                                                                                                                                                                                                              cGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCTCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTNCGTCGAAACGTAAAATCCGGCACGCTAGACATACATCGCCTGTTCAACCATCGACTAGTAGTTGTGCGCTATTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCAt   <  2:208994/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              cGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCAt   <  1:237858/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              cGTGTACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCAt   <  2:855650/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               gtgtACCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGACTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCCACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGAAAGGAGATTCCCCTCGCTGACTAATGCATc  >  2:448807/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                        TGTCCATACGAATGCCGCCCCGCGTGTCATGTTCCATTGCCTGCGCCAGCAAGGCCAGTTCACGAGGATCAAAAATATTCCCTGTTGGATGGTCAGGATTGGAAGAGGTCAAAAATCTCAGCTTACTGTCATAGAGCTGATACTCACCATCGAGGTTTTTATCGATGGCGTTTCTTAAAAATTGCTGCATAGTACGCACGGGAATACTCATCCCCAGCACGCCATACCAGTAGTTATTACTGTCTACCGGAACACTGACGGTAACCTGCGGTTCAGTATTGCTGGCGTGTTCCGGTTGCGAAGTGAACCAGCGTACCGCGCGGTGACGATTTTCTCGTTGTGAATGGCCAATAAACCAGGGTTGGGTGACATAGCCGTAATAACGCGTTGGAACATTGCGCGTAAACAAGGTCGGCTGCGTCGAAACGTAAAATCCGGCACGCGAGACATACATCGCCTGTTCAACCATCGACGAGGAGTTGTGCGCTAGTCGCAGCAAGTAACCAACTTCCAGTGCAGCGGTAATTTCATTGTCGAGGCTTTCATTTTCGCGAGACAGGAGATTCCCCTCGCTGACTAATGCATC  >  NC_000913/2027042‑2027627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑