breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 71,170 T→C S262P (TCG→CCG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
MC JC 260,173 Δ13,782 bp IS5‑mediated proB[ykfC] 19 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 298,885 G→A P684S (CCG→TCG)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 365,306 G→A R334C (CGC→TGC)  lacZ ← beta‑galactosidase
RA 414,163 G→A R531* (CGA→TGA)  sbcC ← ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit
RA 416,276 G→A A226V (GCA→GTA)  sbcD ← ATP‑dependent structure‑specific DNA nuclease ‑ SbcD subunit
RA 421,027 C→T R14R (CGC→CGT proY → putative transporter ProY
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 495,680 C→T F187F (TTC→TTT htpG → chaperone protein HtpG
RA 537,024 C→T P147S (CCG→TCG)  glxR → tartronate semialdehyde reductase 2
RA 579,099 (A)5→4 intergenic (‑206/+85) borD ← / ← ybcV prophage lipoprotein BorD/DUF1398 domain‑containing protein YbcV
RA 579,127 T→C intergenic (‑234/+57) borD ← / ← ybcV prophage lipoprotein BorD/DUF1398 domain‑containing protein YbcV
RA 579,134 T→C intergenic (‑241/+50) borD ← / ← ybcV prophage lipoprotein BorD/DUF1398 domain‑containing protein YbcV
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 627,348 G→A V427M (GTG→ATG)  entE → 2,3‑dihydroxybenzoate‑AMP ligase
RA 721,604 G→A R44C (CGT→TGT)  kdpE ← DNA‑binding transcriptional activator KdpE
JC JC 820,914 IS5 (+) +4 bp coding (122‑125/714 nt) ybhM → Bax1‑I family protein YbhM
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
JC JC 1,105,699 IS1 (+) +9 bp coding (286‑294/312 nt) ymdA → uncharacterized protein YmdA
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
JC JC 1,196,827 IS5 (–) +4 bp intergenic (+45/+37) C0293 → / ← ymfD small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,517,587 2 bp→TT intergenic (+25/‑60) ortT → / → ydcY orphan toxin OrtT/DUF2526 domain‑containing protein YdcY
RA 1,591,711 C→A E156* (GAG→TAG)  hipA ← serine/threonine‑protein kinase toxin HipA
RA 1,600,081 C→T W43* (TGG→TGA lsrK ← autoinducer‑2 kinase
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,712,919 C→T Q51* (CAA→TAA)  dtpA → dipeptide/tripeptide:H(+) symporter DtpA
RA 1,792,541 C→T G64E (GGA→GAA)  nlpC ← NlpC/P60 family lipoprotein NlpC
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,977,360 G→T P162Q (CCG→CAG)  flhC ← DNA‑binding transcriptional dual regulator FlhC
RA 2,023,559 A→G T223A (ACT→GCT)  fliR → flagellar biosynthesis protein FliR
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,083,303 C→T A183V (GCG→GTG)  sbcB → exodeoxyribonuclease I
RA 2,090,780 C→T Q197* (CAA→TAA)  hisG → ATP phosphoribosyltransferase
RA 2,104,024 G→T I162I (ATC→ATA wbbK ← putative glycosyltransferase WbbK
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,120,969 G→A L196L (CTG→TTG)  wcaJ ← UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
JC JC 2,239,316 IS5 (–) +4 bp intergenic (‑27/+31) mglA ← / ← mglB D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit/D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,481,608 A→G L190S (TTA→TCA)  emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,520,199 A→G G158G (GGT→GGC gltX ← glutamate‑‑tRNA ligase
RA 2,710,250 G→A intergenic (‑86/‑170) rseD ← / → nadB rpoE leader peptide/L‑aspartate oxidase
RA 2,745,600 A→G Y107H (TAC→CAC)  rimM ← ribosome maturation factor RimM
RA 2,774,947 G→A G544S (GGT→AGT)  yfjW → uncharacterized protein YfjW
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,959,564 C→T W955* (TGG→TGA recC ← exodeoxyribonuclease V subunit RecC
RA 3,148,900 G→A G335D (GGC→GAC)  gpr → L‑glyceraldehyde 3‑phosphate reductase
RA 3,256,301 G→A S117S (TCC→TCT cyuA ← putative L‑cysteine desulfidase CyuA
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,444,922 G→C T103S (ACC→AGC)  rpsE ← 30S ribosomal subunit protein S5
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,507,249 C→T D30N (GAC→AAC)  yhfT ← uncharacterized protein YhfT
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,679,581 G→A G55R (GGA→AGA)  kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,729,441 C→T noncoding (105/160 nt) xylZ ← small RNA XylZ
RA 3,730,560 C→T W69* (TGG→TAG)  xylA ← xylose isomerase
RA 3,931,779 T→C F155S (TTT→TCT)  kup → K(+):H(+) symporter Kup
RA 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho
RA 4,026,650 G→A D42N (GAT→AAT)  fre → NAD(P)H‑flavin reductase
RA 4,153,836 G→A Q338* (CAA→TAA)  argE ← acetylornithine deacetylase
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,479,944 G→A G72E (GGG→GAG)  yjgN → inner membrane protein YjgN
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257746 259052 1307 7 [5] [5] 6 [frsA]–[phoE] [frsA],crl,insB9,insA9,crl,[phoE]
* * ÷ NC_000913 576653 577297 645 6 [5] [5] 6 [nmpC] [nmpC]
* * ÷ NC_000913 685727 686217 491 6 [5] [5] 6 [gltK]–[gltJ] [gltK],[gltJ]
* * ÷ NC_000913 1196831 1197384 554 7 [0] [5] 6 [ymfD] [ymfD]
* * ÷ NC_000913 1423571 1424634 1064 6 [5] [4] 6 [rzoR]–[trkG] [rzoR],[trkG]
* * ÷ NC_000913 1978484 1979270 787 15 [0] [0] 16 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423867–3424234 3424438–3424238 5–572 6 [5] [5] 6 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 19796 =NA (NA)27 (0.570) 25/482 0.8 75.0% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 361656 9 (0.190)coding (205/612 nt) lacA galactoside O‑acetyltransferase
* ? NC_000913 = 25589114 (0.290)25 (0.520) 25/484 0.8 64.1% intergenic (‑175/‑86) pepD/gpt peptidase D/xanthine‑guanine phsophoribosyltransferase
?NC_000913 273955 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 258675NA (NA)31 (0.650) 29/484 0.6 73.8% noncoding (1/768 nt) IS1 repeat region
?NC_000913 362204 = 11 (0.230)coding (976/1254 nt) lacY lactose permease
* ? NC_000913 273955 =NA (NA)26 (0.540) 25/484 0.8 96.3% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 677814 1 (0.020)coding (400/1428 nt) djlB J domain‑containing protein DjlB
* ? NC_000913 = 275149NA (NA)34 (0.710) 32/484 0.5 79.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 579184 = 9 (0.190)coding (411/411 nt) ybcV DUF1398 domain‑containing protein YbcV
* ? NC_000913 = 67617518 (0.380)11 (0.230) 11/484 2.1 37.9% coding (373/978 nt) ybeQ Sel1 repeat‑containing protein YbeQ
?NC_000913 687851 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 78168812 (0.250)17 (0.460) 16/376 1.1 67.6% intergenic (+36/‑397) lysQ/nadA tRNA‑Lys/quinolinate synthase
?NC_000913 781845 = 7 (0.190)intergenic (+193/‑240) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 12994985 (0.100)7 (0.150) 7/476 2.7 74.0% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1299499 =NA (NA)7 (0.150) 6/460 2.9 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1300694 = 0 (0.000)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 14119246 (0.130)21 (0.490) 20/432 1.0 85.1% coding (25/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 1434985 = 2 (0.050)intergenic (‑579/+200) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 1469296 =NA (NA)19 (0.400) 19/484 1.2 61.3% noncoding (1/1221 nt) IS30 repeat region
?NC_000913 = 4117985 12 (0.250)coding (106/846 nt) glpF glycerol facilitator
* ? NC_000913 1743490 =17 (0.360)25 (0.520) 24/484 0.9 59.5% coding (34/1374 nt) mdtK multidrug efflux pump MdtK
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 174349317 (0.360)14 (0.290) 13/484 1.8 45.2% coding (37/1374 nt) mdtK multidrug efflux pump MdtK
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 19784830 (0.000)15 (0.310) 15/484 1.6 100% intergenic (‑286/+35) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 1979270NA (NA)14 (0.290) 14/484 1.7 28.0% noncoding (1/768 nt) IS1 repeat region
?NC_000913 2176322 = 36 (0.750)intergenic (‑1/+28) gatZ/gatY putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone/tagatose‑1,6‑bisphosphate aldolase 2
* ? NC_000913 1979271 =0 (0.000)14 (0.310)
+TTAAGTAATTGAGTG
14/454 1.6 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 2996360 = NA (NA)intergenic (+2504/+12) ygeI/insD4 protein YgeI/IS2 insertion element protein InsB
* ? NC_000913 2288919 =NA (NA)14 (0.290) 13/484 1.8 42.4% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 2484281 19 (0.400)coding (527/615 nt) evgA DNA‑binding transcriptional activator EvgA
* ? NC_000913 2288919 =NA (NA)58 (1.220) 52/482 0.1 95.1% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 3131469 = 3 (0.060)coding (940/1068 nt) yghQ putative transport protein YghQ
* ? NC_000913 2288919 =NA (NA)27 (0.570) 26/484 0.8 71.0% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 3206571 = 11 (0.230)coding (109/912 nt) ttdA L(+)‑tartrate dehydratase subunit alpha
* ? NC_000913 2288919 =NA (NA)17 (0.360) 17/484 1.4 68.0% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3362227 8 (0.170)coding (116/675 nt) yhcA putative fimbrial chaperone YhcA
* ? NC_000913 2288919 =NA (NA)8 (0.170) 8/484 2.6 27.6% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3890573 21 (0.440)coding (338/1248 nt) tnaB tryptophan:H(+) symporter TnaB
* ? NC_000913 = 2290113NA (NA)25 (0.520) 25/484 0.8 58.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 2484278 = 18 (0.380)coding (524/615 nt) evgA DNA‑binding transcriptional activator EvgA
* ? NC_000913 = 2290113NA (NA)35 (0.730) 33/484 0.5 77.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3206574 10 (0.210)coding (112/912 nt) ttdA L(+)‑tartrate dehydratase subunit alpha
* ? NC_000913 = 2290113NA (NA)30 (0.630) 28/484 0.7 79.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 3843039 = 8 (0.170)coding (751/1335 nt) adeQ adenine transporter
* ? NC_000913 = 2290113NA (NA)14 (0.290) 14/484 1.7 40.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 3890570 = 21 (0.440)coding (335/1248 nt) tnaB tryptophan:H(+) symporter TnaB
* ? NC_000913 2514274 =NA (NA)51 (1.080) 49/478 0.1 73.9% noncoding (2/1345 nt) IS186 repeat region
?NC_000913 = 2955099 18 (0.380)coding (905/3543 nt) recB exodeoxyribonuclease V subunit RecB
* ? NC_000913 = 2515616NA (NA)26 (0.550) 26/478 0.8 63.4% noncoding (1344/1345 nt) IS186 repeat region
?NC_000913 2955094 = 15 (0.320)coding (910/3543 nt) recB exodeoxyribonuclease V subunit RecB
* ? NC_000913 2537833 =16 (0.340)14 (0.290) 14/484 1.7 46.7% coding (85/735 nt) yfeS PF05406 family protein YfeS
?NC_000913 = 2997691 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 253783716 (0.340)16 (0.340) 14/484 1.7 50.0% coding (89/735 nt) yfeS PF05406 family protein YfeS
?NC_000913 2996361 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 25586984 (0.080)54 (1.170) 49/468 0.1 84.5% intergenic (‑19/‑160) eutB/intZ ethanolamine ammonia‑lyase subunit alpha/putative phage integrase IntZ
?NC_000913 2565489 = 16 (0.350)coding (1396/1404 nt) eutA ethanolamine ammonia‑lyase reactivase EutA
* ? NC_000913 2765412 =8 (0.170)14 (0.360) 13/396 1.4 74.6% intergenic (‑259/+101) abpA/yfjM anti‑bacteriophage protein/protein YfjM
?NC_000913 = 2765419 3 (0.080)intergenic (‑266/+94) abpA/yfjM anti‑bacteriophage protein/protein YfjM
* ? NC_000913 3583428 =NA (NA)36 (0.760) 36/482 0.4 83.7% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 4542049 7 (0.150)coding (13/597 nt) fimE regulator for fimA
* ? NC_000913 = 3584195NA (NA)35 (0.730) 34/484 0.4 76.1% noncoding (768/768 nt) IS1 repeat region
?NC_000913 4546532 = 11 (0.230)coding (1437/2637 nt) fimD type I fimbriae usher protein
* ? NC_000913 3652036 =NA (NA)47 (0.980) 46/484 0.2 85.5% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3843042 8 (0.170)coding (748/1335 nt) adeQ adenine transporter
* ? NC_000913 = 3653230NA (NA)25 (0.520) 22/484 1.0 67.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 3655259 = 12 (0.250)coding (644/648 nt) yhiD inner membrane protein YhiD
* ? NC_000913 3901122 =30 (0.630)13 (0.270) 13/484 1.8 30.2% coding (1099/1617 nt) bglH carbohydrate‑specific outer membrane porin, cryptic
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 390112632 (0.670)10 (0.210) 10/482 2.2 23.8% coding (1095/1617 nt) bglH carbohydrate‑specific outer membrane porin, cryptic
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 4117984 =12 (0.250)15 (0.320) 15/480 1.6 55.6% coding (107/846 nt) glpF glycerol facilitator
?NC_000913 4507459 = NA (NA)noncoding (1221/1221 nt) IS30 repeat region