Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 421,027 C→T R14R (CGC→CGT)  proY → putative transporter ProY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913421,0270CT81.4% 93.0 / 15.4 43R14R (CGC→CGT) proYputative transporter ProY
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base T (22/13);  total (27/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTA  >  NC_000913/420784‑421269
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
cTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATAc                                                                                                                                                                                                                                             >  1:360597/1‑251 (MQ=255)
              tACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACt                                                                                                                                                                                                                                <  1:113808/250‑1 (MQ=255)
                  gCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGtt                                                                                                                                                                                                                           <  2:42102/251‑1 (MQ=255)
                                            tAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTC‑GGAATTGATGGACAGTAA‑GAACAAGCTAAAGCGTGGG‑CTACGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTAc                                                                                                                                                                                                 >  2:125872/1‑251 (MQ=255)
                                             aaTGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATg                                                                                                                                                                                                                                     >  2:219870/1‑214 (MQ=255)
                                             aaTGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATg                                                                                                                                                                                                                                     <  1:219870/214‑1 (MQ=255)
                                                         ggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGACACGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCAcc                                                                                                                                                                                                             >  1:240520/1‑226 (MQ=255)
                                                           tgATGGTGGGTCTGGCAATTATCTGGGATCGCGCGGCAGGTCGTCAGGGGACCTCAAGCGCTCACTAAATCACTGAACAATTGATTTACCCACGGAGCTGCGATGACCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTCA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGAAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGcc                                                                                                                                                                                  >  2:37081/1‑251 (MQ=255)
                                                                      cTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAAGAAGCTAAAGCGTGGG‑CTAAGTACCCGCCACATACGCTTTATGGCACTGGGGTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGc                                                                                                                                                                       >  2:71111/1‑251 (MQ=255)
                                                                        ggCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATgg                                                                                                                                                                        <  2:480656/248‑1 (MQ=255)
                                                                               aTCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCg                                                                                                                                                                >  1:134054/1‑249 (MQ=255)
                                                                                   gggATCGCGCGGCAGGGCGTCAGGTGACCTCCAGCGCTAACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCg                                                                                                                                                            <  1:125872/249‑1 (MQ=255)
                                                                                   gggACCGCGGGGCAGGTCGTAAGGTACCCCCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCAGGGGGCGGCGATGCCCCGTGGTTTTTTTTTGTGTGGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTCCCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGc                                                                                                                                                             <  1:413705/248‑1 (MQ=255)
                                                                                        cgcgcgGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGtgttgt                                                                                                                                                      <  1:169072/250‑1 (MQ=255)
                                                                                             ggCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCt                                                                                                                                                >  2:395452/1‑251 (MQ=255)
                                                                                                                          aCTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATc                                                                                                                   >  1:66378/1‑251 (MQ=255)
                                                                                                                                   cTGAACATTTGTTTTAACAACGGGGCGGAGATGCCCCGTGGTTTTTTATTGGGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGAAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTAtgcgt                                                                                                          >  1:57466/1‑251 (MQ=255)
                                                                                                                                    tGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTAtgcgtg                                                                                                         >  2:362550/1‑251 (MQ=255)
                                                                                                                                     gAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTGTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTGGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTAtgcgtgc                                                                                                        <  2:36692/251‑1 (MQ=255)
                                                                                                                                         aTTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTg                                                                                                    <  2:134054/251‑1 (MQ=255)
                                                                                                                                            tGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGCATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAAtt                                                                                                                                                                                                                   >  1:70782/1‑137 (MQ=255)
                                                                                                                                                          ggCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCtata                                                                                                                                             >  2:84900/1‑193 (MQ=255)
                                                                                                                                                          ggCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CGAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCtata                                                                                                                                             <  1:84900/193‑1 (MQ=255)
                                                                                                                                                              gNGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGTAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACACTTTATGGCGCTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGGTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGAGATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATa                                                                                 <  2:443412/249‑1 (MQ=255)
                                                                                                                                                               cGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAAcc                                                                              >  2:222420/1‑251 (MQ=255)
                                                                                                                                                                   cccggtgttttttttGTTGTGT‑GA‑GGGTTAAGGAATTG‑TGGTAAGTAAAGAAAAAACAAAAG‑GTGGGTCTAAGTCCCCTCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGTCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgcc                                                                         <  2:57466/242‑1 (MQ=255)
                                                                                                                                                                               tttATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGCAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTAGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTCTATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAAGGTCGGTACCTAACCCGGCCGCCAGCTCttt                                                              >  2:94699/1‑251 (MQ=255)
                                                                                                                                                                                 tATTGTGTTAGTGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTtct                                                            >  2:315689/1‑251 (MQ=255)
                                                                                                                                                                                    tgtgCTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGCTTTACGGTTCGGCAGACGCCATCAAAATGGCCGCTCCGCGCGTGTTGTTGGCCTATATTATCGGTGGCACCGCGGCGTCTATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTcgc                                                         >  2:163234/1‑251 (MQ=255)
                                                                                                                                                                                       gtTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAACGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGtt                                                      >  1:127615/1‑251 (MQ=255)
                                                                                                                                                                                         tGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTa                                                     <  1:222420/250‑1 (MQ=255)
                                                                                                                                                                                                       aTTGATGGAAAGTAA‑GACCAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGTCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGTAAAAcc                                       <  1:151751/251‑1 (MQ=255)
                                                                                                                                                                                                          gATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACATCg                                    >  1:489473/1‑251 (MQ=255)
                                                                                                                                                                                                           aTGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCgg                                   >  2:430033/1‑251 (MQ=255)
                                                                                                                                                                                                                aaGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGc                              >  1:254992/1‑251 (MQ=255)
                                                                                                                                                                                                                aaGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGc                              >  1:166681/1‑251 (MQ=255)
                                                                                                                                                                                                                        aaCAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAgg                       >  1:237386/1‑251 (MQ=255)
                                                                                                                                                                                                                         aaaaGCTAAAGCGTGGG‑TAAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACAt                                                                                  <  1:471999/189‑1 (MQ=255)
                                                                                                                                                                                                                         aCAAGCTAAAGCGTGGG‑CTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACAt                                                                                  >  2:471999/1‑191 (MQ=255)
                                                                                                                                                                                                                          cAAGCTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCATAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGtt                     >  2:10870/1‑251 (MQ=255)
                                                                                                                                                                                                                              cTAAAGCGTGGG‑CTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACAt                 >  1:50828/1‑251 (MQ=255)
                                                                                                                                                                                                                                            tAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTgggcc    >  2:80968/1‑248 (MQ=255)
                                                                                                                                                                                                                                              aGTACCCGTCGCTTACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTGCGGGTCGGCAGACGCCATCAAAATGGCCGGGCCGAGCGTGTGGTGGGCCTAGATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGGCGGTACATATCCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATGACCGGCTGGACCTa  <  2:464754/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
CTGGGCGCAGCGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTA‑GGAATTGATGGAAAGTAA‑GAACAAGCTAAAGCGTGGG‑CTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTGGACCTA  >  NC_000913/420784‑421269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑