breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 255 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,848,3000CT79.7% 116.8 / 28.5 59G126E (GGG→GAG) ‡ydjAputative oxidoreductase YdjA
*NC_0009131,147,7920TA79.6% 125.7 / 25.4 55S58T (TCG→ACG) plsXputative phosphate acyltransferase
*NC_000913309,9850TA79.5% 104.5 / 18.8 44G14G (GGA→GGTecpBputative fimbrial chaperone EcpB
*NC_0009132,546,8040TA79.5% 93.7 / 18.5 45D44E (GAT→GAAmurPN‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
*NC_0009133,729,4400CT79.5% 93.5 / 19.0 45noncoding (106/160 nt)xylZsmall RNA XylZ
*NC_0009133,411,6890GT79.4% 58.0 / 11.6 35E13* (GAA→TAA) yhdJDNA adenine methyltransferase
*NC_000913395,1690CT79.3% 60.5 / 10.0 29E373E (GAG→GAAampHpeptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
*NC_000913789,5800CA79.3% 123.8 / 30.7 58E134* (GAA→TAA) galKgalactokinase
*NC_0009131,848,3010CT79.3% 116.8 / 28.8 59G126R (GGG→AGG) ‡ydjAputative oxidoreductase YdjA
*NC_000913780,6240CT79.2% 95.7 / 24.3 53noncoding (71/76 nt)lysTtRNA‑Lys
*NC_0009132,570,2690GA79.1% 173.1 / 41.6 88P23P (CCC→CCTeutJputative ethanolamine utilization chaperonin EutJ
*NC_0009132,002,8250CT78.8% 62.9 / 17.0 33G261E (GGA→GAA) fliCflagellar filament structural protein
*NC_0009132,577,9370TC78.7% 105.9 / 20.1 47E147E (GAA→GAGmaeBmalate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
*NC_0009131,038,4770GA78.6% 90.5 / 19.1 43A246A (GCG→GCAappCcytochrome bd‑II subunit 1
*NC_0009131,522,5830CT78.6% 108.1 / 31.6 56Q161Q (CAG→CAAyncDputative TonB‑dependent outer membrane receptor
*NC_0009133,6450GA78.4% 73.1 / 17.5 37W282* (TGG→TAG) thrBhomoserine kinase
*NC_0009132,041,2780GA78.1% 57.2 / 17.0 32intergenic (+160/‑97)msrQ/zinTmembrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
*NC_000913708,7740GA78.0% 80.4 / 19.6 41A147A (GCG→GCAchiPchitooligosaccharide outer membrane channel
*NC_0009131,541,0370AG78.0% 82.1 / 21.0 41I518I (ATT→ATCnarZnitrate reductase Z subunit alpha
*NC_0009132,926,7720CA78.0% 122.9 / 28.4 59G155G (GGC→GGAppnNnucleotide 5'‑monophosphate nucleosidase

Marginal new junction evidence (lowest skew 10 of 18 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4163082 =49 (1.030)6 (0.130) 6/482 3.0 10.9% coding (189/1101 nt) trmA tRNA m(5)U54 methyltransferase
?NC_000913 = 4499511 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 308832433 (0.690)7 (0.150) 6/484 3.0 17.5% coding (41/1395 nt) galP galactose:H(+) symporter
?NC_000913 = 4508679 NA (NA)noncoding (1/1221 nt) IS30 repeat region
* ? NC_000913 1978503 =NA (NA)6 (0.130) 6/484 3.0 14.6% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 2176330 35 (0.730)intergenic (‑9/+20) gatZ/gatY putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone/tagatose‑1,6‑bisphosphate aldolase 2
* ? NC_000913 = 174349921 (0.440)6 (0.130) 6/484 3.0 22.2% coding (43/1374 nt) mdtK multidrug efflux pump MdtK
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1034134 =49 (1.030)6 (0.130) 6/480 3.0 10.9% coding (881/1794 nt) hyaB hydrogenase 1 large subunit
?NC_000913 1469296 = NA (NA)noncoding (1/1221 nt) IS30 repeat region
* ? NC_000913 3088323 =34 (0.720)5 (0.110) 5/480 3.2 12.8% coding (40/1395 nt) galP galactose:H(+) symporter
?NC_000913 4507459 = NA (NA)noncoding (1221/1221 nt) IS30 repeat region
* ? NC_000913 = 12994974 (0.080)6 (0.130) 5/480 3.2 60.2% intergenic (+252/‑70) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 = 1300694 NA (NA)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 2579070 (0.000)5 (0.110) 5/468 3.2 100% intergenic (+8/+16) crl/insB9 RNA polymerase holoenzyme assembly factor Crl/IS1 family transposase B
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl RNA polymerase holoenzyme assembly factor Crl
* ? NC_000913 = 3187426NA (NA)5 (0.100) 5/484 3.3 11.4% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 3367749 39 (0.820)intergenic (+107/‑78) yhcF/yhcG DUF1120 domain‑containing protein YhcF/DUF1016 domain‑containing protein YhcG
* ? NC_000913 = 280111NA (NA)4 (0.080) 4/480 3.5 10.5% noncoding (181/181 nt) IS30 repeat region
?NC_000913 3088323 = 34 (0.720)coding (40/1395 nt) galP galactose:H(+) symporter