Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,444,922 G→C T103S (ACC→AGC)  rpsE ← 30S ribosomal subunit protein S5

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,444,9220GC87.0% 172.7 / 17.2 54T103S (ACC→AGC) rpsE30S ribosomal subunit protein S5
Reads supporting (aligned to +/- strand):  ref base G (4/3);  new base C (26/21);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

GACCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTA  >  NC_000913/3444677‑3445155
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         
gacCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCt                                                                                                                                                                                                                                      <  2:96643/251‑1 (MQ=255)
        gCACTGCGGGTTTGAGTAATTTTCATAGACTTTGCCATGGTTTATTTCCCCAGAATTTATTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCACTAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGCACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACAGGCGATGATACCGCTACCTTCTGAAGc                                                                                                                                                                                                                              >  2:51881/1‑251 (MQ=255)
           cTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCgg                                                                                                                                                                                                                           >  2:102319/1‑251 (MQ=255)
                ggTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCa                                                                                                                                                                                                                      <  1:341658/251‑1 (MQ=255)
                    tGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGa                                                                                                                                                                                                                   >  2:99770/1‑250 (MQ=255)
                      aGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGACCGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAAt                                                                                                                                                                                                                 >  2:480607/1‑250 (MQ=255)
                          aTTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATAcgcg                                                                                                                                                                                                            <  2:99731/251‑1 (MQ=255)
                          aTTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCAAGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATAcgcg                                                                                                                                                                                                            >  1:475448/1‑251 (MQ=255)
                               aaTAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAAc                                                                                                                                                                                                       >  1:282648/1‑251 (MQ=255)
                               aaTAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCAAGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGCTACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAAc                                                                                                                                                                                                       >  1:473405/1‑251 (MQ=255)
                                 tAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGCAccc                                                                                                                                                                                                     >  2:160794/1‑251 (MQ=255)
                                   gTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAAACCgt                                                                                                                                                                                                   >  1:314944/1‑251 (MQ=255)
                                          ccATGGTTTATTTCCACAGAATTTATTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGATGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGACAGCCGGCTGCATGCATACGCGAGAACCCGTGTGAac                                                                                                                                                                                             >  2:277448/1‑250 (MQ=255)
                                              ggTTTATTTCCCCAGAATTTCTTCAACGGATTTCCCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCtt                                                                                                                                                                                        >  1:416584/1‑251 (MQ=255)
                                              ggTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCtt                                                                                                                                                                                        <  1:387225/251‑1 (MQ=255)
                                                               tttCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGc                                                                                                                                                                          >  1:233201/1‑248 (MQ=255)
                                                                       aCGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCgtg                                                                                                                                                                                                  >  2:49864/1‑216 (MQ=255)
                                                                       aCGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCgtg                                                                                                                                                                                                  <  1:49864/216‑1 (MQ=255)
                                                                           aTTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTc                                                                                                                                                           <  2:254405/251‑1 (MQ=255)
                                                                                    ggttGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACg                                                                                                                                                  >  2:51359/3‑251 (MQ=255)
                                                                                    gCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACg                                                                                                                                                  <  2:364439/251‑1 (MQ=255)
                                                                                        ggCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTaa                                                                                                                                              <  1:223084/251‑1 (MQ=255)
                                                                                         gCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAAt                                                                                                                                             >  2:113811/1‑251 (MQ=255)
                                                                                          cAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATc                                                                                                                                            >  1:204750/1‑251 (MQ=255)
                                                                                                          agaATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCtt                                                                                                                            <  1:107156/251‑1 (MQ=255)
                                                                                                          agaATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCtt                                                                                                                            >  1:271939/1‑251 (MQ=255)
                                                                                                               tCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTc                                                                                                                        <  1:470710/250‑1 (MQ=255)
                                                                                                                                       ttGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTgg                                                                                                >  1:84235/1‑250 (MQ=255)
                                                                                                                                                ccACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCAcgc                                                                                      >  2:36407/1‑251 (MQ=255)
                                                                                                                                                       gATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTAc                                                                               >  2:240906/1‑251 (MQ=255)
                                                                                                                                                        aTCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTAcc                                                                              <  1:458511/251‑1 (MQ=255)
                                                                                                                                                           gggTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGACCACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGt                                                                            >  1:21189/1‑250 (MQ=255)
                                                                                                                                                            ggTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTa                                                                           >  2:328442/1‑250 (MQ=255)
                                                                                                                                                               tggtggAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTaacc                                                                        <  2:74347/250‑1 (MQ=255)
                                                                                                                                                                ggaggAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTACAGAAAGGAGCGCATTGCACCACAGGCGATGATACCGGTACCTTCGGAAGCCGGCGGCGTGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCCGCGCGACGATAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGAGATTTACCGTaacca                                                                       >  2:190757/4‑250 (MQ=255)
                                                                                                                                                                         aTAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGa                                                                                                                                                                                                                   >  1:140082/1‑101 (MQ=255)
                                                                                                                                                                         aTAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGa                                                                                                                                                                                                                   <  2:140082/101‑1 (MQ=255)
                                                                                                                                                                         aTAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAAcgc                                                             >  1:156325/1‑251 (MQ=255)
                                                                                                                                                                         aTAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAAcgc                                                             <  1:480607/251‑1 (MQ=255)
                                                                                                                                                                                           gTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGtt                                                                                                                                                >  1:419930/1‑150 (MQ=255)
                                                                                                                                                                                           gTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGtt                                                                                                                                                <  2:419930/150‑1 (MQ=255)
                                                                                                                                                                                             tATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACt                                         >  2:245777/1‑251 (MQ=255)
                                                                                                                                                                                              aTGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTa                                        <  2:328894/251‑1 (MQ=255)
                                                                                                                                                                                               tGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGAc                                                          >  2:190441/1‑232 (MQ=255)
                                                                                                                                                                                               tGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGAc                                                          <  1:190441/232‑1 (MQ=255)
                                                                                                                                                                                                  aCCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGCATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGt                                    >  1:271871/1‑251 (MQ=255)
                                                                                                                                                                                                         cGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCt                             <  1:140327/251‑1 (MQ=255)
                                                                                                                                                                                                               ccAGAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAg                       <  1:99770/251‑1 (MQ=255)
                                                                                                                                                                                                                  gAACGGCGCGCATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGGAACCAAAACCAACGCGGCCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGgag                    <  2:282648/251‑1 (MQ=255)
                                                                                                                                                                                                                            cATTGCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGac          <  1:271199/251‑1 (MQ=255)
                                                                                                                                                                                                                                gCACCACCGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGaccacc      <  1:239900/251‑1 (MQ=255)
                                                                                                                                                                                                                                    caccGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTa  <  2:156325/251‑1 (MQ=255)
                                                                                                                                                                                                                                      ccGGCGATGATACCGCTCCCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCttt                                                                                                                                                                                       <  1:19941/68‑1 (MQ=255)
                                                                                                                                                                                                                                      ccGGCGATGATACCGCTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCttt                                                                                                                                                                                       >  2:19941/1‑68 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         
GACCGATTGCACTGCGGGTTTGAGTAATTTTAATAGTCTTTGCCATGGTTTATTTCCCCAGAATTTCTTCAACGGATTTACCACGCTTGGCAGCGACCATTTCTGGAGAATTCATATTTTCCAGGCCATCAATAGTTGCACGAACCACGTTGATCGGGTTGGTGGAACCATAGGCTTTAGCCAGAACGTTATGAACCCCAGCGACTTCCAGAACGGCGCGCATTGCACCACCGGCGATGATACCGGTACCTTCGGAAGCCGGCTGCATGAATACGCGAGAACCCGTGTGAACACCTTTAACAGGGTGTTGCAGAGTGCCGTTATTCAGCGCGACGTTAATCATATTGCGACGGGCTTTTTCCATCGCTTTCTGGATCGCTGCTGGAACTTCACGCGCTTTACCGTAACCAAAACCAACGCGACCGTTACCATCGCCAACTACAGTCAGAGCTGTGAAGGAGAAAATACGACCACCTTTA  >  NC_000913/3444677‑3445155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑