| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 414,163 | G→A | R531* (CGA→TGA) | sbcC ← | ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 414,163 | 0 | G | A | 93.6% | 138.2 / 1.7 | 47 | R531* (CGA→TGA) | sbcC | ATP‑dependent structure‑specific DNA nuclease ‑ SbcC subunit |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (30/14); total (32/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
AGCTGGCGTTCGTGCTCATCTTGTGCATCCAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTC > NC_000913/413919‑414404 | aGCTGGCGTTCGTGCTCATCTTGTGCATCCAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTg > 1:486403/1‑251 (MQ=255)aGCTGGCGTTCGTGCTCATCTTGTGCATCCAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTg < 1:256098/251‑1 (MQ=255) cGTTCGTGCTCATCTTGTGCATCCAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCCCGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAAc > 2:169732/1‑251 (MQ=255) gTTCGTGCTCATCTTGTGCATCCAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCGAGACTGCTTAACg > 1:18854/1‑251 (MQ=255) gTTCGTGCTCATCTTGTGCATCCAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACg > 1:285242/1‑251 (MQ=255) gTGCTCATCTTGTGAATCCAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGTGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGcc > 1:368135/1‑249 (MQ=255) gCATCCAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGCCTGATTAACGCCAGGCTCCAGCGCCt < 1:211305/250‑1 (MQ=255) ccAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATa < 1:412518/250‑1 (MQ=255) gCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGcc < 1:259861/251‑1 (MQ=255) gTTGAATATCGTCCAGTGGATGCAAGGTGATATTGGGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCGGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCCGCGCATGATACGCATCGAcc > 2:107209/1‑251 (MQ=255) gCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGATGAGTAAGAGCTTGCACATATTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGa > 1:20443/1‑250 (MQ=255) cAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACc‑ac > 1:235286/1‑251 (MQ=255) tGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGt > 1:234688/1‑251 (MQ=255) aGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAgg > 2:10018/1‑251 (MQ=255) aagggtttgctCATCTTGTCGGGGGTTTTGCGCTTCGTTTTCACCACGCGGAAGCTGCTTTGTTAGGGCGGCCAGTTCCCCACGAGGCGTCGCTCTTTCTTCCCCGACCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGAAATAAAGACTTATAAACGCCAGGCCCCGGCGCCTGATCCCCCCCGCCCGCGGGGGGGCTGGTGAAACC‑AAAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCtt < 2:278296/245‑1 (MQ=255) gctCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGGGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGtt > 2:481681/1‑251 (MQ=255) tCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGt < 2:17407/201‑1 (MQ=255) tCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGt > 1:17407/1‑201 (MQ=255) gcTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGAGCTTCCTGCTCGc > 1:436132/1‑250 (MQ=255) ttttCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCCCCTTCTTCTCCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACaaa < 1:192950/172‑1 (MQ=255) ttttCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACaaa > 2:192950/1‑172 (MQ=255) tcaCGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTAG‑TTTTCCAGCGCCAGTAATAAAGACTGATTAACGCCAGGCTACAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCGGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTcac > 2:455985/1‑251 (MQ=255) aaGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCa > 2:135269/1‑250 (MQ=255) aGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCa > 1:417293/1‑249 (MQ=255) tttttttttgggtgtcatgTTGCCCGCGTAGCGCCTCACCCTTTACACCGA‑CATTTTTAC‑TTCGGTTTTCCAGCCCCATAAACCAAGTCTGATTACCGCCAGCCCCCTGCCCTTGAGACGCCTCGCCCGCCGGGTGGTTGGTGGACCC‑AAAAAGTGGGCGAGGCTGCCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGt < 1:468381/230‑1 (MQ=255) ttgtggtggggctccggTTGCACACTTAGCGTTGCCCTTTCTACACGGAG‑TTTTTAACCTTCG‑TTTTCCGGCGCCAGAAAAAAAGTCGGATTACCGCCAGGCCCCAGCCCCTAATACGCCTCG‑CCGGCGGGGGGGCGGGGGAACCCAAAAATTGGGCAAGGCTACCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTtgct < 2:368135/234‑1 (MQ=255) gTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGt > 1:364532/1‑251 (MQ=255) gTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATAAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTAACATCGGCAAGTTGCTGCGt > 2:140995/1‑251 (MQ=255) tGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGtcttt > 1:114146/1‑251 (MQ=255) cccACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCAAGCGCCAGTAGTCAAGACTGATTAACGCCAGGCTCCAGCGCATGATACGCCTCGACCGCCGGGTGGCTGGAGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGAGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCt > 1:485411/1‑251 (MQ=255) cgtggcgtccccctttcttcACCGAGTTTTTAAAC‑TTCT‑TTTTCCAGCCCCAGAAATCAAGACTTATTAACGCCGGGCCCCGGCCCCTGATACGCCGGGCCCGCGGGGTGGCTGGGGAAACC‑AAAAGGTGGGCAGGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGgcg < 1:458437/237‑1 (MQ=255) gTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCAAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGgcgc > 2:28026/1‑251 (MQ=255) gTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTc > 2:103347/1‑251 (MQ=255) gTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACAGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTc > 1:3612/1‑251 (MQ=255) tCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCGGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCg > 1:308425/1‑251 (MQ=255) cACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGtt > 1:189090/1‑250 (MQ=255) ttctaccccgatttttttAAC‑TTCT‑TTTGCCAGCGCAAGTAATCAAAGCTAATTAACGCCAGCCCCAAGCCCCTAACACGCCTGGACCGCCGGGTGGCTGGGGGACCC‑ACAAAGTGGGAAAGGCTGCCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGt < 2:157344/237‑1 (MQ=255) tttttAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACGGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGCTGCTGCGTCTTTTCTTTAAAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTg > 2:234475/1‑251 (MQ=255) tttttactttCG‑TTTTCCAGCCCCAGTAATAAAGATTAATAAACGCCGGCCCCCAGCCCCTGATGCGCCTCGCCCGCGGGGTGGGTGGGGGAACC‑ACAAGGGGGGAAGGGTTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGtt < 2:234688/242‑1 (MQ=255) tttAAC‑TTCG‑TTTTCCAGCGACAGAAATAAAGGCTAATTAACGCCAGGCCCCAGCCCCTAACACCCCTCGACCGCGGGGTGGCGGGTGGACCC‑AAAAAGTGGGCAAGGTTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGc < 2:285242/250‑1 (MQ=255) tAAC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTc > 2:162831/1‑251 (MQ=255) aaC‑TTCG‑TTTTCCAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCt > 2:114297/1‑251 (MQ=255) cG‑TTTCCCAGCGCCAGTAAACAAGGCTGATTAACGCCGGGCTCCAGCGCCTGATGCGCCCCGACCGCGGGGTGGCTGGGGGACCC‑ACAAAGTGGGCAGGGCTGCCCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCt < 1:151640/251‑1 (MQ=255) ccAGCGCCAGTAAGCGAGACTGATTAACGCGAGTCTCCAGCCCCTGATACGCCTCGCCCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGAAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTg < 1:366394/251‑1 (MQ=255) cAGCGCCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGC‑TCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGAc > 1:442150/1‑251 (MQ=255) gcCAGTAATCAAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACAtt > 1:397827/1‑251 (MQ=255) ccAGTAATCAAGGCTGATTAACGCCAGGTTCCAGCGCCTGATACGCCTCGACCGACGAATGCCTGGTGGAACC‑ACAAAGTGGACAAGGCTGACCCGCCTGTAACTGTGCACGATGAGCTTCCAGAGTTTTGATGCGCGATTACTGCTCGCAAATGGTTTTAACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGAGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTc > 2:215981/1‑251 (MQ=255) | AGCTGGCGTTCGTGCTCATCTTGTGCATCCAGCCACGGTTGAATATCGTCCAGTGGCTGCAAGGTGATATTGAGGCTGGCCGTGACGGCTTGCCATTGTTGAGTAAGTGCTTGCTCATCTTGTCGGAGGCTTTGCGCTTCGTTTTCATCACGCTGAAGCTGCTTTGTTATGGCGTCCAGTTGCCCACGTAGCGTCGCACCTTCTTCACCGAGCTTTTTAAC‑TTCG‑TTTTCCAGCGCCAGTAATCGAGACTGATTAACGCCAGGCTCCAGCGCCTGATACGCCTCGACCGCCGGGTGGCTGGTGGAACC‑ACAAAGTGGGCAAGGCTGACCCGCCTGTAACTGTGCACGTTGAGCTTCCAGCGTTTTGATGCGCGCTTCCTGCTCGCAAATGGTTTTCACATCGGCAAGTTGCTGCGTCTTTTCTTTATAACGCTGGCGCATTTCGTTAAGTGCGGCGTTACGTTGCGTCTGTTCTTGCGTGACATTC > NC_000913/413919‑414404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |