Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,256,301	G→A	S117S (TCC→TCT)	cyuA ←	putative L‑cysteine desulfidase CyuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,256,301	0	G	A	81.0%	127.1 / 27.6	64	S117S (TCC→TCT)	cyuA	putative L‑cysteine desulfidase CyuA
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base A (28/23);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01

CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGC  >  NC_000913/3256057‑3256543                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   cAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCCCACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACt                                                                                                                                                                                                                                              <  1:138415/251‑1 (MQ=255)  aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACtt                                                                                                                                                                                                                                             >  2:32748/1‑251 (MQ=255)   aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACttt                                                                                                                                                                                                                                            >  1:450578/1‑251 (MQ=255)       ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTcccc                                                                                                                                                                                                                                        <  1:110139/251‑1 (MQ=255)        gACTTAATTGACGAACTTCAGGCTCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCACCGGAGACTCTTGCTCGCCCTCTGCCCCACACGCCTGCTGGGTAAACACCAAACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑CATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCATGGATCTTAACCGAGACTTTccc                                                                                                                                                                                                                                         >  2:73899/1‑249 (MQ=255)           ttCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCccgccg                                                                                                                                                                                                                                    >  1:137044/1‑251 (MQ=255)              aTTGACGAACTGCAGGATCTCAGCCAGGTTCGTTCTGAAAAGCCCCGTCAGGGGAGATTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACCCCATCGTGCGT‑CTCGATATCCACAATGTTGG‑TATGCCCGCCGACGATGGTAACACACGCCCACTTCTCACCGTTCCAGCCTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCca                                                                                                                                                                                                                                 <  2:146442/251‑1 (MQ=255)                tGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCnagc                                                                                                                                                                                                                               >  2:437962/1‑251 (MQ=255)                 gACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGAcagc                                                                                                                                                                                                                               >  1:386878/1‑250 (MQ=255)                  aCGAACTTCAGGATCTCAGCCAGCGTCTTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCcagcag                                                                                                                                                                                                                             <  1:229512/251‑1 (MQ=255)                   caaccTTCAGGATCTCAGCCAGCGTCGTTCTGGAAACCCCCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGT‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCCCCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCAGCGCAAGTTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGt                                                                                                                                                                                                                            <  2:223224/247‑1 (MQ=255)                      aCTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTATTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGGGACACACGCCCACTTATCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAAACTTTCCCCGCCGCCAGCCGTGCt                                                                                                                                                                                                                         >  1:349039/1‑251 (MQ=255)                        ttCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATAGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCttt                                                                                                                                                                                                                       >  2:477565/1‑251 (MQ=255)                               cctcAGCCGGCGTCGGTCGGGAAAGCACCGTCAGGGGAGCCTCTTGCTCGCCCTCTGCCACACCCGCCGGTGGGGTAAACACCCCACCATCGTGCGT‑CTCGATATGCACAATGTGGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCGGGTTCTTAACAGAGACTTGCCCCGCCGCCAGCAGTGCTTTGGCATc                                                                                                                                                                                                                 <  2:295010/249‑1 (MQ=255)                                ctcAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTACGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGCGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCgg                                                                                                                                                                                                               >  1:160473/1‑251 (MQ=255)                                  gagccagcTTCGTTCTGGAAAGCACAGCCAGCGGAGACTCTGGCCCGCCCGCTGCAACACTCGCCTGCTGGGTAAACACCACACCATCTGGCGT‑CATTATAGGCACAATGTGGG‑TATGCCCGCCGACGATGGTGACACACTCCCACTTCTCACAGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCTGGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCg                                                                                                                                                                                                                <  1:37524/247‑1 (MQ=255)                                  cagccaggGTCGTTTGGGAAGGCACCGTCAGCGGAGACTCTTGCTCGCCCTCGGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCCT‑CTCGATAGGCACAATGTTGG‑TATGCCCGCCGACGGTGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTGCATCGCAAGGTTCCTGAATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCa                                                                                                                                                                                                             <  2:128867/251‑1 (MQ=255)                                     ccagcGTCGTTCGGGAAAGCACCGTCAGCGGAGCCTCTTGCCCGCCCTCTGCCCCACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGTTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAAtt                                                                                                                                                                                                          <  1:109497/251‑1 (MQ=255)                                          gtcgtTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTg                                                                                                                                                                                                     >  1:83923/1‑251 (MQ=255)                                                   gAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACCCACGCCTGCTGGTTAAACCCCACACCATCGTGCTT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCTCAAGGTTCCTGAATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGc                                                                                                                                                                                            <  1:316591/251‑1 (MQ=255)                                                    aaaGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCg                                                                                                                                                                                           <  1:262098/251‑1 (MQ=255)                                                     aggccccgccggCGGAGACGCTTGCTCGCCCTCTGCCGCACACGCCTTCTGGGAAAACCCCCCACCAGCTTGCTT‑CTCGATATACACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGCCTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGt                                                                                                                                                                                          <  2:215294/240‑1 (MQ=255)                                                       ccACCGCCAGCGGGGACTCTTCCTCGCCCCCTGCACCACACGCCTGCTGGGTAAACCCCACACCTTCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGCCGATGGTGACACACGCCCCCTTCCCCCCGTTCCAGACTTTGGCGCGGGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTCCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCt                                                                                                                                                                                        <  2:401524/250‑1 (MQ=255)                                                            gTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTc                                                                                                                                                                                     >  2:210569/1‑249 (MQ=255)                                                            gTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTc                                                                                                                                                                                     >  1:263403/1‑249 (MQ=255)                                                                           tGCTCGCCCTCTGCCCCACACGCCTTCTGGGAAAACACCACACCATCGTGCGT‑CTCGATATGCCCAATGTTGG‑TAGGCCCGCCGACGATGGTGACACACGCCCACTTCTCCCCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCg                                                                                                                                                                      <  1:119178/249‑1 (MQ=255)                                                                            gCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGc                                                                                                                                                                    <  1:429993/250‑1 (MQ=255)                                                                                      tGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTc                                                                                                                                                         >  1:206476/1‑251 (MQ=255)                                                                                          acacccGCCTGCTGGGTAAACACCACCCCAGCG‑GTGTGCTGGATATGCACAATGTGGG‑GAGGCCCGCCGACGATGGTGACACACGCCCACTTCCCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGTTTTCACCGCAGGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCTCTGTTGCTTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCAcc                                                                                                                                                     <  2:386878/251‑1 (MQ=255)                                                                                           cgcccGCCTGCTGGGTAAACCCCGCGCCAGCGTGCGT‑CTGGGTATGCACAATGTGGG‑GCTGCCCGCCGACGATGGTGACACACGCCCTCTGCTCACCTTGCCAGACTTTGGCCCGTGAGAAGACGTTTTCATCGCAAGGTCCCTGGAGCTTAACAGAGATTTACCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCt                                                                                                                                                    <  2:166567/247‑1 (MQ=255)                                                                                               cGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACg                                                                                                                                                >  2:480717/1‑251 (MQ=255)                                                                                                        ggaaaaccccaccacacggTGCGA‑CTCAATTTGCACAA‑GTTGGGTATGCCCGCCACCGATTGTGACCCGCGCCCACTTCTCACCGTTCCAGACGTTGGCGCGTGAGAAGAGTATTGCATCGCAAGGTTCCTGCATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTATCGCCCCCAgcg                                                                                                                                       <  2:193351/233‑1 (MQ=255)                                                                                                           aaaCACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGcgccg                                                                                                                                    >  1:101435/1‑251 (MQ=255)                                                                                                               cccccaccaGCGTGCGT‑CGCGATATGCCAAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCCCCGTTCCAGATTTGGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATTTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGcgccgccgc                                                                                                                                <  2:325873/247‑1 (MQ=255)                                                                                                                          gTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGcc                                                                                                                     >  1:231141/1‑251 (MQ=255)                                                                                                                               t‑ctcGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCccacc                                                                                                                >  1:120110/1‑251 (MQ=255)                                                                                                                                   cGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCAtt                                                                                                             >  1:67940/1‑251 (MQ=255)                                                                                                                                     atatGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTACAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCACCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCACTCGGCCACCCCACCATTcc                                                                                                           >  2:60821/1‑251 (MQ=255)                                                                                                                                           acaATGTTGG‑TAGCCCCGCCGACGATGGTGACACACCCCCCCTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCACCGAAAGTTTCCTGGATCTTAACAGAGACTTCCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGcc                                                                                                     <  2:263403/251‑1 (MQ=255)                                                                                                                                                        tGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTACAGCCTTTGGCGCGTGAGAAGAGGATTTCATCGCCAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTTCCGGGAACGGTGAc                                                                                         >  2:465118/1‑251 (MQ=255)                                                                                                                                                          cccGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGc                                                                                       >  2:471184/1‑251 (MQ=255)                                                                                                                                                            cgccgACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGccc                                                                                     >  1:346842/1‑251 (MQ=255)                                                                                                                                                             gccgAAGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCAACCATTCCCGTGCCGGGAACGGGGACGccc                                                                                     >  2:310305/1‑250 (MQ=255)                                                                                                                                                                 acgaTGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGAcc                                                                                <  1:373570/251‑1 (MQ=255)                                                                                                                                                                    aTGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGtt                                                                             <  1:437962/251‑1 (MQ=255)                                                                                                                                                                           cacacGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatc                                                                      >  1:175638/1‑251 (MQ=255)                                                                                                                                                                            acacGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatc                                                                      >  1:249625/1‑250 (MQ=255)                                                                                                                                                                                gCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAtt                                                                 >  1:298280/1‑251 (MQ=255)                                                                                                                                                                                           aCCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGATCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGACATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAccc                                                      >  1:193484/1‑251 (MQ=255)                                                                                                                                                                                             cGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGGGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAAccc                                                      >  1:39246/1‑249 (MQ=255)                                                                                                                                                                                               ttCCAGACTTTGGCGCGTGATAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGCCTTCCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAgg                                                   <  2:123719/250‑1 (MQ=255)                                                                                                                                                                                                  cAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAgg                                                   >  1:134666/1‑247 (MQ=255)                                                                                                                                                                                                  cAGACTTGGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAgg                                                   <  2:134666/247‑1 (MQ=255)                                                                                                                                                                                                        ngggCGCGTGAGAAGGGGATTTCACCGCAAGGTTCCTGGATCTAAACAGAGCCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTac                                            <  2:450578/246‑1 (MQ=255)                                                                                                                                                                                                               gTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCACCGCCAGCAGTGCATTGGCATCAGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCACTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGGTCTCCACCAGCTTTGCCGCAACCCCGGCTTCTACACGTTCNAcc                                  >  2:442011/1‑251 (MQ=255)                                                                                                                                                                                                                tGAGAAGCTGATTCCATCGCAAGGTTCCTTTATCTTAATAGAGACTGTACCCGCGGCCAGCAGTGCTTGGGCATCGGCAATTGCATGCGCTGTTGCGTCTTGCAGCACTGCCAGCCCGGCGTTGGCATTTCCGCCGAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTCCCGGGAACGGTGATGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAcc                                  <  2:160473/250‑1 (MQ=255)                                                                                                                                                                                                                tGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCg                                 >  1:482088/1‑251 (MQ=255)                                                                                                                                                                                                                 gagaAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGGACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCgg                                >  2:215253/1‑251 (MQ=255)                                                                                                                                                                                                                 gagaAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCg                                 <  2:231141/250‑1 (MQ=255)                                                                                                                                                                                                                             tCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaa                                                          >  2:292375/1‑213 (MQ=255)                                                                                                                                                                                                                             tCATCGCAAGGTTCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaa                                                          <  1:292375/213‑1 (MQ=255)                                                                                                                                                                                                                                       gTTCCTGGATCTTAACGGAGACTTCCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcagca          <  1:60821/251‑1 (MQ=255)                                                                                                                                                                                                                                        ttCCTGGATCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcagca          <  2:298280/250‑1 (MQ=255)                                                                                                                                                                                                                                               aTCTTAACAGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgc  >  1:463183/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGT‑CTCGATATGCACAATGTTGG‑TATGCCCGCCGACGATGGTGACACACGCCCACTTCTCACCGTTCCAGACTTTGGCGCGTGAGAAGAGGATTTCATCGCAAGGTTCCTGGATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGC  >  NC_000913/3256057‑3256543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑