| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,120,969 | G→A | L196L (CTG→TTG) | wcaJ ← | UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,120,969 | 0 | G | A | 87.3% | 140.7 / 12.3 | 55 | L196L (CTG→TTG) | wcaJ | UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase |
| Reads supporting (aligned to +/- strand): ref base G (4/3); new base A (26/22); total (30/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.51e-01 | |||||||||||
GCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATACCACG > NC_000913/2120728‑2121214 | gCACGTTTGAGCAGGCGGTTAACCACGGAAAGCTGCGTGTCGTAAAGCGTCACCACCGGTACGCCGTTCATCTCTTCGATGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGCTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCAGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGGTTTCGCGTCCTCGACCAACTGTTGCAg < 2:14500/251‑1 (MQ=255) ttGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTAc < 1:372831/250‑1 (MQ=255) aGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑Gc > 1:239518/1‑251 (MQ=255) cGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTAACC‑GCCCAGTc > 2:371492/1‑251 (MQ=255) ggTTACCCCCGGAAAGCGGCGTGTCGTAAAGCGGCGCCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCGTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCg < 1:390174/251‑1 (MQ=255) ccccGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGTCCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGaa < 1:383493/251‑1 (MQ=255) ccGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCC‑GCCCAGTCGTTAGnacc > 2:444090/1‑249 (MQ=255) gAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAAcgcc > 1:241629/1‑251 (MQ=255) gTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCTCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGt < 1:286137/251‑1 (MQ=255) gTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGt > 1:102253/1‑251 (MQ=255) aaaGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGAACCGAACAGGTGGTGTCCGGCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCTTCCTCGACCAGCTGTTGCAGGTTTCCC‑TCCCAGTCGGTAGAAACGCCGCCCGTTTTCGGGTCg > 1:342407/1‑251 (MQ=255) gCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTa > 1:187627/1‑250 (MQ=255) ccGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGccc > 2:100755/1‑251 (MQ=255) ggTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGCATGTTGACGGTCAACACCACGGGGATCAGCACCCCCGAACAGGTGGGGTCCGCCAGTTGTTAGCCCCGTTTTTTCACTCGCGGGCCGACGCACATCTGCCTCGCGATATAGCCGTTATGAATCTTGCACGCTTTCGCGTCCTCGACGAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCCCCCGGCTTCGGGCCGTGGTAAACGccccc > 2:65874/1‑249 (MQ=255) gCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACtt > 2:394762/1‑251 (MQ=255) ttCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAAcc > 1:483150/1‑251 (MQ=255) aggcgacaaTGAAGAATGGTGAAGGCGAAGACGTCGGGGATCAGCCGCACCGAACCGGAAGGGTCCGCCAGTTGATCGACCAGCTTTTTAACTCGCGCGACGTCGCACATTTGCATCGCGATATAGCAGCAATGAATCTTGCACGCCCTCGCGTCATCGCCCAACTGTTGCCGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCTGGTTTAGGGTCGTGGTCAACGCCAACTACTTCACACCCTAACCNCgg > 2:441259/8‑251 (MQ=255) gCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCt < 1:471344/251‑1 (MQ=255) gTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTgg > 2:244947/1‑251 (MQ=255) tGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGt > 1:370138/1‑251 (MQ=255) gagaATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGa < 1:248300/251‑1 (MQ=255) gaATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAg < 1:290560/251‑1 (MQ=255) aaTGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGc > 2:383230/1‑251 (MQ=255) tGAAGGTAAATACGACGGGGATAAGCAGCACCGAACAGGTGGTGTACGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGGCGCACATTTGCATCGAGATATAAACGTTATGAATCATGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCAGGTTTCGGGTCGTGGTAAACACCCAACACTTCAAACCCTAACCACGGCTGGTACCGGAAGCTCTcc > 2:74541/1‑251 (MQ=255) ggTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATc > 1:84751/1‑250 (MQ=255) gACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCAttt < 1:444090/251‑1 (MQ=255) gggATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGCTACGGAAGCTCTCCATCCGCATTTACCcggc > 1:256603/1‑251 (MQ=255) cagcagCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCt > 1:47094/1‑250 (MQ=255) agcaCCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGTCGGCTAAATc > 1:397118/1‑251 (MQ=255) cccGAACAGGTGGTGTCCGCCAGTTGATGTACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATcccc < 1:388088/250‑1 (MQ=255) ccGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGc > 1:377204/1‑233 (MQ=255) ccGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGc < 2:377204/233‑1 (MQ=255) gAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGc < 2:241629/251‑1 (MQ=255) aaCAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGGCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCCGGCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGcc < 1:109331/251‑1 (MQ=255) aGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCAcc > 2:22085/1‑251 (MQ=255) ggtggtGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCAccc > 1:136066/1‑250 (MQ=255) cAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCt < 2:102253/251‑1 (MQ=255) gTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTg < 1:111937/251‑1 (MQ=255) ccAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAAc > 2:113391/1‑161 (MQ=255) ccAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACGTCAAAc < 1:113391/161‑1 (MQ=255) cACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGc < 1:244947/250‑1 (MQ=255) gcgcgcCGGCGTACATTTGAATCGCGATATAGACGTTATGAATCTTGCCCTCTTTCGCGTCCCCGACCAACTGTTGCAGGTTCCCC‑GCCCAGTCGTTAGAAAAGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCTATTCAAACCCTACCCACGGCTGGTTACTGAAGCTCTCCATCAGTATGTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGcagccag < 2:426498/251‑1 (MQ=255) cgcCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCTTCCTCTACCAACTGTTGCAGGTTACCC‑GCGCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTTCCCGGCGGCGAAGTCCCCCGTCACCGCGCCCATGCGCTTGTTATAGCCATGATTACGCAGCCAgccc < 1:65874/251‑1 (MQ=255) ccGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAgcccgc > 1:412111/1‑251 (MQ=255) aTCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCACTGCGAATac > 1:352894/1‑250 (MQ=255) cgcgATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATacac < 1:305820/250‑1 (MQ=255) tgATATAGACGTTATTAATCTTGCCCGCTTTCGCGTCCTCTACCAACTGTTGC‑CGTTACCCCGCCCAGTCGTTAGAAACTCCGCCCGGTTTCGGGTCGGGTTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACg < 2:370138/248‑1 (MQ=255) gATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAAc < 1:371492/251‑1 (MQ=255) ataGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACgg < 1:383230/250‑1 (MQ=255) cGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAacc > 2:89602/1‑251 (MQ=255) aaTCTTGCCCGCTTTCGCGTCCGCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTATAACCGCCGCCCGGTTTCGGGTCGTGGTAAACGACCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTc < 1:25910/251‑1 (MQ=255) cTTGCCCGCTTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACCCGAACGGCAAACCACCAGTCCg > 2:81638/1‑250 (MQ=255) gCCCGATTTCGCGTCCTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGNTGc > 2:436311/1‑251 (MQ=255) ccTCGACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCGAACCACGGCTGGTTACGGAAGCTCTCCATCATCATTTGCCCGGCGGCTAAATCCCCCGCCGCCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATa < 1:481266/250‑1 (MQ=255) gACCAACTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCCTCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACTGCAAACCACCAGTCCCATGCTGGTCAGCGCATACCACg > 1:466024/1‑251 (MQ=255) | GCACGTTTGAGCAGGCGGTTAACCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTCATCTCTTCGAGGCGTGAATGGAGAATGTTGAAGGTAAAGACGTCGGGGATCAGCAGCACCGAACAGGTGGTGTCCGCCAGTTGATGGACCAGTTTTTTCACTCGCGCGCCGTCGCACATTTGCATCGCGATATAGACGTTATGAATCTTGCCCGCTTTCGCGTCCTCGACCAGCTGTTGCAGGTTACCC‑GCCCAGTCGTTAGAAACGCCGCCCGGTTTCGGGTCGTGGTAAACGCCCACCACTTCAAACCCTAACCACGGCTGGTTACGGAAGCTCTCCATCAGCATTTGCCCGGCGGCTAAATCCCCCGCCACCGCGACCATGCGCTTGTTATAGCCATGATTACGCAGCCAGCCCGCCCCAATGCGAATACACGAACGGCAAACCACCAGTCCGATGCTGGTCAGCGCATACCACG > NC_000913/2120728‑2121214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |