Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,026,650	G→A	D42N (GAT→AAT)	fre →	NAD(P)H‑flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,026,650	0	G	A	87.8%	123.2 / 12.4	49	D42N (GAT→AAT)	fre	NAD(P)H‑flavin reductase
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (27/16);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

GATGTTGTCGCGCATATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATT  >  NC_000913/4026413‑4026889                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                gATGTTGTCGCGCATATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACa                                                                                                                                                                                                                                     >  2:6221/1‑250 (MQ=255)  aTGTTGTCGCGCATATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACaa                                                                                                                                                                                                                                    >  2:238471/1‑250 (MQ=255)       gtCGCGCATATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAACCGTc                                                                                                                                                                                                                               <  1:55350/250‑1 (MQ=255)         cgcgCATATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGt                                                                                                                                                                                                                            <  2:320904/251‑1 (MQ=255)                             gggATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGAc                                                                                                                                                                                                            <  1:238471/247‑1 (MQ=255)                                  gAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGa                                                                                                                                                                                                   <  1:365909/251‑1 (MQ=255)                                   aaCTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGa                                                                                                                                                                                                   >  2:408053/1‑250 (MQ=255)                                    aCTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCTTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGac                                                                                                                                                                                                  >  2:147928/1‑249 (MQ=255)                                               ttAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATc                                                                                                                                                                                      >  2:27001/1‑251 (MQ=255)                                                  acaacGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGCAAAAGGGGTTATCg                                                                                                                                                                                     >  2:399826/1‑249 (MQ=255)                                                       ggTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGc                                                                                                                                                                               >  1:81534/1‑250 (MQ=255)                                                       ggTAAAAGAGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGATCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGcc                                                                                                                                                                              >  2:294266/1‑250 (MQ=255)                                                        gTAAGAGCTCCTGATGCGGGGTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCAGCATCGTGCTAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGAGATGAATGAGCGCGACAAACGTACGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTAAACGAGCTGc                                                                                                                                                                               >  2:257167/1‑249 (MQ=255)                                                           aaaGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATAtt                                                                                                                                                                          <  2:303588/251‑1 (MQ=255)                                                                 ccttaTGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCTCATGTCAACCTTAAGCTGTAAAGTGACCTCGGTAGTAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCCTTCTCAATGACTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCt                                                                                                                                                                    <  2:466450/247‑1 (MQ=255)                                                                   tGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCCTGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGACTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCttt                                                                                                                                                                  >  1:286846/1‑250 (MQ=255)                                                                    gATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCt                                                                                                                                                                 <  1:408053/251‑1 (MQ=255)                                                                              tgtttTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAAcc                                                                                                                                                       >  1:83577/1‑251 (MQ=255)                                                                                          tttATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGaa                                                                                                                                             >  2:253686/1‑249 (MQ=255)                                                                                               cGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGGCGCGGCCTTTTCTTGTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTGCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTc                                                                                                                                      <  2:358269/251‑1 (MQ=255)                                                                                                gATCCGACATAGAACGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGCCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTc                                                                                                                                      >  2:465743/1‑250 (MQ=255)                                                                                                     gACAGAGCAAGCGAATGACAACCTTAAGCTGGAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGAAGCGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGTTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGGCATGGAc                                                                                                                                >  1:409846/1‑251 (MQ=255)                                                                                                      aCAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTGCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACTCGAAAGCAGTCATGGAcc                                                                                                                               <  2:182144/251‑1 (MQ=255)                                                                                                                              aaGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCaaaat                                                                                                        <  1:253686/250‑3 (MQ=255)                                                                                                                                      aaGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGAc                                                                                               >  2:10051/1‑251 (MQ=255)                                                                                                                                       aGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCTAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGAc                                                                                               >  2:196380/1‑250 (MQ=255)                                                                                                                                         tGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGGCGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACAtt                                                                                            >  1:178227/1‑251 (MQ=255)                                                                                                                                             cTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTcccc                                                                                        >  2:364451/1‑251 (MQ=255)                                                                                                                                               cGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAA‑GATCATCAAATCGTGGTCGACATTCCCCACg                                                                                     <  1:218750/251‑1 (MQ=255)                                                                                                                                                             aCGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAACAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATAATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCt                                                                        >  2:174943/1‑251 (MQ=255)                                                                                                                                                               ggATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgc                                                                      >  2:413363/1‑251 (MQ=255)                                                                                                                                                               ggATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTgc                                                                      >  2:292649/1‑251 (MQ=255)                                                                                                                                                                        atatCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATTGTAGTGATGAATGAGCGCGACAAACGGCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCTAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGTGCGACATTCCCCACGGAGAAGCGTGGCTGCGCcatgat                                                              <  2:215445/250‑1 (MQ=255)                                                                                                                                                                         tatCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGatgatg                                                             >  1:129214/1‑250 (MQ=255)                                                                                                                                                                                   gCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGAAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCg                                                  <  2:23106/251‑1 (MQ=255)                                                                                                                                                                                                cgcgTCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCC‑ACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTgc                                    >  1:372677/1‑251 (MQ=255)                                                                                                                                                                                                cgcgGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTg                                     <  2:329351/251‑1 (MQ=255)                                                                                                                                                                                                      cttttcttttCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTgcgggcg                               >  1:359785/1‑251 (MQ=255)                                                                                                                                                                                                         ttcttttcGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGggcggc                             >  1:101755/1‑250 (MQ=255)                                                                                                                                                                                                           cttttcGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCAcc                          >  2:58779/1‑251 (MQ=255)                                                                                                                                                                                                                cGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGt                      <  1:6221/250‑1 (MQ=255)                                                                                                                                                                                                                  tGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTtct                   >  1:199705/1‑251 (MQ=255)                                                                                                                                                                                                                      ggTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATgg                                                                                                                                  <  2:234405/136‑1 (MQ=255)                                                                                                                                                                                                                      ggTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATgg                                                                                                                                  >  1:234405/1‑136 (MQ=255)                                                                                                                                                                                                                          aGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGGTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCGGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGcc           >  2:387173/1‑251 (MQ=255)                                                                                                                                                                                                                                tGATGGTAGTGAAGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTc      <  2:81534/250‑1 (MQ=255)                                                                                                                                                                                                                                 gATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCg     <  1:196380/250‑1 (MQ=255)                                                                                                                                                                                                                                  aTGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGAt   >  1:385931/1‑251 (MQ=255)                                                                                                                                                                                                                                    ggTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGAtt  <  2:83577/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                GATGTTGTCGCGCATATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCCCGCTCGATT  >  NC_000913/4026413‑4026889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑