Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,6380TC95.7% 73.6 / ‑6.2 23intergenic (‑10/‑190)iclR/metHDNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (9/13);  total (9/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGT  >  NC_000913/4223404‑4223880
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    
cTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGGTTTC‑CGGGGATTGGGGCGACCATGACAGACTCcttttttttttttgtgg                                                                                                                                                                                                                                      >  1:460323/1‑240 (MQ=255)
              ccTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCG‑CTTTGCGGGGATGGGTGGGACCAAGACAGGCTCCCTTTTCTTTTTCGTGGaaagaaattttctt                                                                                                                                                                                                                        >  1:180031/1‑240 (MQ=255)
                       gCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTTGAAATCATTTT‑CATTTTTATTGt                                                                                                                                                                                                               <  1:252566/250‑1 (MQ=255)
                        cATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTa                                                                                                                                                                                                             <  2:147922/251‑1 (MQ=255)
                                   gCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGTCAATCCACTCCAGTGAGTTCAGGCCACGCGTTAAAGACTGAACCTGTCGAATCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGTCGCG‑TTGCGCAGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTTTATCGTTGAAATCATGTT‑CTTTTTTATTGTTTTCTAAT‑GCAAt                                                                                                                                                                                                 <  2:43100/251‑1 (MQ=255)
                                                         gggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTTGCGGGGATGGGGGGGGCCATGACAGTCTCCTTTTTTTTTTTCGTGGGAATCATTTT‑TATTTTTTTTTTTTGCTAAT‑GCAATAGTTACTGAAATGTTCCGATgt                                                                                                                                                                           >  1:358189/1‑250 (MQ=255)
                                                             aaCCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTa                                                                                                                                                                       <  1:105243/251‑1 (MQ=255)
                                                               cccGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAAt                                                                                                                                                                     <  1:306563/251‑1 (MQ=255)
                                                                      tgttgCGCTCGTTCCGTTAGTGCCACACTGCCATTGGATTCTGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGTTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTTAACTGATCCGATGAGTTATTGTTGaa                                                                                                                                                               <  2:48042/250‑1 (MQ=255)
                                                                                gTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCCCGGGACTGGGGGGGGCCAAGAGAGACTCCTTTTTTTGGATCGGGGGAAACATTTTTCTTTTTTTTTTTTA‑ATAATTGCA‑TAGTTTCTGAACTGATACGATGAGTTaaatttgtaaaaatctcttg                                                                                                                                                    >  2:301447/1‑233 (MQ=255)
                                                                                  tCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGt                                                                                                                                                   <  1:301447/250‑1 (MQ=255)
                                                                                    cGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGc                                                                                                                                                <  2:466915/251‑1 (MQ=255)
                                                                                           gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGtggtgg                                                                                                                                         <  2:159788/251‑1 (MQ=255)
                                                                                                acTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTTGCGGGGATGGGGGGGACCATGACAGTCTCCTTTTTCTGTATCGTGGGAATCATTTT‑TATTTTTTTTTTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGGGTGGTGGTCggc                                                                                                                                    >  1:251042/1‑249 (MQ=255)
                                                                                                   gCCATTGGATTCGGCAATCCACTCCAGTAATTGCAGGCCACGCGTTAAAGACGGAACCTGGCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGGTACGGAACTGAGCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCtt                                                                                                                                   <  1:295927/249‑1 (MQ=255)
                                                                                                                      cACTCCAGTAATTGCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTGTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAAGAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTGGCGTGGTGGTCGCTGTTACCACAGATGCGTTTATGc                                                                                                              <  2:237544/251‑1 (MQ=255)
                                                                                                                                                 aaaGTCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACTGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTaa                                                                                   <  1:79333/251‑1 (MQ=255)
                                                                                                                                                   aGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTTCCGCG‑GTTCGCGGGGATGGGGGGGAACATGACAGTTTCCTTTTTTTGTTTTGTGGAAATCCTTTT‑TATTTTTATTGTTTGCTAAT‑GCAATAGTTACTGAACTGATCCGAAGAGTTTATGGTTAACAAATATCATGTTTCGTGGGGGGCGCTTTTACCACAGATGCGTTTATTCCAGTATGGTTTGTTGAATTTTTATTTAAt                                                                                 >  1:192144/1‑251 (MQ=255)
                                                                                                                                                     aCTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCt                                                                               <  2:340742/251‑1 (MQ=255)
                                                                                                                                                                   gTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTc                                                                 <  2:315974/251‑1 (MQ=255)
                                                                                                                                                                                          gggTTTTCTGCCGCG‑GTTCGCGGAAATGGGTGCGACCCTGACAGTCTCCTTTATCTGTATCGTGGAAACCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACCGATCCGATGAGTTAATGTTGAACAAATCTCATGTTTCGTGGTGGTCGCTTTTACCACAGTTGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAACGTGTCGGGAGCAAGTGagagacgcaa                                            >  2:480568/1‑243 (MQ=255)
                                                                                                                                                                                                          tttCGCGGGAATGGGTGCGACCATGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACtgcgtg                           >  1:150891/1‑251 (MQ=255)
                                                                                                                                                                                                                                aTGACAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCt     >  1:341319/1‑251 (MQ=255)
                                                                                                                                                                                                                                   aCAGTCTCCTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTggg  >  1:159135/1‑250 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    
CTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCG‑TTTCGCGGGAATGGGTGCGACCATGACAGTCTCTTTTTTCTGTATCGTGGAAATCATTTT‑CATTTTTATTGTTAGCTAAT‑GCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGCTTTTACCACAGATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGGTTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGT  >  NC_000913/4223404‑4223880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑