mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 155,463 | A→T | *247K (TAA→AAA) | yadV ← | putative fimbrial chaperone YadV |
| MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
| MC JC | 519,143 | Δ14,682 bp | [ybbO]–[allZ] | [ybbO], [tesA], ybbA, ybbP, rhsD, ybbC, ylbG, selU, allS, allA, [allR], [allZ] | |
| RA | 535,337 | T→C | A474A (GCT→GCC) | gcl → | glyoxylate carboligase |
| RA | 536,173 | T→C | L155P (CTC→CCC) | hyi → | hydroxypyruvate isomerase |
| RA | 536,492 | A→G | intergenic (+6/‑94) | hyi → / → glxR | hydroxypyruvate isomerase/tartronate semialdehyde reductase 2 |
| RA | 536,549 | T→C | intergenic (+63/‑37) | hyi → / → glxR | hydroxypyruvate isomerase/tartronate semialdehyde reductase 2 |
| MC JC | 536,617 | Δ14,712 bp | [glxR]–[ybcF] | [glxR], ybbW, allB, ybbY, glxK, allE, allC, allD, fdrA, ylbE, ylbF, [ybcF] | |
| RA | 557,635 | A→T | L36Q (CTG→CAG) | folD ← | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase |
| MC JC | 565,054 | 44181 bp→TGA | [intD]–insL2 | 52 genes [intD], renD, insE3, insF3, renD, emrE, ylcJ, ybcK, ybcL, ybcM, ylcH, ybcN, ninE, ybcO, rusA, ylcG, quuD, nmpC, insH2, nmpC, essD, rrrD, rzpD, rzoD, borD, ybcV, ybcW, ylcI, nohD, tfaD, tfaX, appY, ompT, envY, ybcH, nfrA, nfrB, cusS, cusR, cusC, cusF, cusB, cusA, pheP, ybdG, nfsB, ybdF, ybdJ, ybdK, sokE, hokE, insL2 |
|
| RA | 670,635 | A→T | L324Q (CTG→CAG) | holA ← | DNA polymerase III subunit delta |
| RA | 1,048,596 | G→A | G32G (GGC→GGT) | gfcC ← | capsule biosynthesis GfcC family protein |
| MC JC | 1,048,805 | Δ48,085 bp | [gfcB]–ycdU | 45 genes [gfcB], gfcA, insA4, insB4, cspH, cspG, ymcF, ymcE, gnsA, yccM, torS, torT, torR, torC, torA, torD, cbpM, cbpA, yccE, agp, yccJ, wrbA, ymdF, rutG, rutF, rutE, rutD, rutC, rutB, rutA, rutR, putA, ymdG, putP, efeO, efeB, phoH, pgaD, pgaC, pgaB, pgaA, dgcT, insF4, insE4, ycdU |
|
| JC JC | 1,105,376 | IS2 (–) +5 bp | intergenic (+83/‑34) | csgC → / → ymdA | curlin chaperone/uncharacterized protein YmdA |
| RA | 1,196,861 | A→G | intergenic (+79/+6) | C0293 → / ← ymfD | small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD |
| MC JC | 1,196,867 | Δ27,041 bp | [ymfD]–ymgJ | 37 genes [ymfD], ymfE, lit, intE, xisE, ymfH, ymfI, ymfJ, ymfK, ymfT, ymfL, ymfM, ymfN, ymfR, beeE, ymfQ, ycfK, tfaP, tfaE, pinE, mcrA, iraM, ymgK, ymgL, ycgX, bluR, bluF, ycgZ, ymgA, ariR, ymgC, pdeG, ymgF, ymgD, ymgG, ymgI, ymgJ |
|
| MC JC | 1,299,499 | Δ1,199 bp | insH21 | insH21 | |
| RA | 1,301,917 | C→T | Q246Y (CAG→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
| RA | 1,301,919 | G→T | Q246Y (CAG→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
| RA | 1,337,394 | A→G | S522G (AGC→GGC) | acnA → | aconitate hydratase 1 |
| RA | 1,628,332 | T→C | intergenic (+69/‑20) | ydfG → / → rspR | 3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR |
| MC JC | 1,628,340 | Δ10,127 bp | rspR–[cspI] | 17 genes rspR, ydfZ, ydfI, ydfJ, ynfT, ydfK, pinQ, tfaQ, stfQ, nohA, ynfO, ydfO, gnsB, ynfN, ynfR, ynfQ, [cspI] |
|
| RA | 1,638,571 | G→A | H33Y (CAT→TAT) | cspI ← | cold shock‑like protein CspI |
| RA | 1,640,045 | +C | coding (13/534 nt) | rrrQ ← | putative lysozyme |
| MC JC | 1,640,057 | Δ7,591 bp | [rrrQ]–[dicC] | 18 genes [rrrQ], [ydfR], essQ, ynfU, cspB, cspF, ynfS, ydfT, ydfU, rem, hokD, relE, relB, ydfV, flxA, ydfW, ydfX, [dicC] |
|
| RA | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
| JC JC | 2,016,437 | Δ2 bp :: IS186 (–) +8 bp :: Δ1 bp | coding (570‑577/687 nt) | fliH → | flagellar biosynthesis protein FliH |
| RA | 2,032,423 | G→A | V219V (GTC→GTT) | yedJ ← | putative HD superfamily phosphohydrolase YedJ |
| RA | 2,173,363 | Δ2 bp | intergenic (‑490/+918) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| RA | 2,867,454 | 2 bp→AA | coding (97‑98/993 nt) | rpoS ← | RNA polymerase sigma factor RpoS |
| RA | 2,985,726 | A→G | intergenic (‑134/‑121) | yqeF ← / → yqeG | putative acyltransferase/putative transporter YqeG |
| RA | 2,985,816 | T→C | intergenic (‑224/‑31) | yqeF ← / → yqeG | putative acyltransferase/putative transporter YqeG |
| MC JC | 2,985,842 | Δ47,769 bp | yqeG–[idi] | 39 genes yqeG, yqeH, yqeI, yqeJ, yqeL, yqeK, ygeG, ygeH, ygeI, insD4, insC4, ygeQ, glyU, actS, xdhA, xdhB, xdhC, ygeV, ygeW, ygeX, ygeY, hyuA, yqeA, yqeB, yqeC, mocA, ygfK, ssnA, ygfM, xdhD, xanQ, guaD, ghxQ, ygfS, ygfT, uacT, yqfG, yqfH, [idi] |
|
| MC JC | 3,110,591 | Δ24,234 bp | [yghD]–yghT | 20 genes [yghD], yghE, yqhJ, yghF, yghG, pppA, yghJ, glcA, glcB, glcG, glcF, glcE, glcD, glcC, yghO, insH9, yghQ, yghR, yghS, yghT |
|
| RA | 3,486,205 | C→A | T29K (ACG→AAG) | crp → | DNA‑binding transcriptional dual regulator CRP |
| RA | 3,560,455 | +G | intergenic (+589/+167) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
| JC JC | 3,707,484 | +GA :: IS3 (+) +4 bp | coding (219‑222/1608 nt) | dppA ← | dipeptide ABC transporter periplasmic binding protein |
| JC JC | 3,720,136 | IS3 (–) +3 bp :: +TCA | coding (88‑90/213 nt) | cspA → | cold shock protein CspA |
| JC JC | 3,890,411 | IS1 (–) +9 bp | coding (176‑184/1248 nt) | tnaB → | tryptophan:H(+) symporter TnaB |
| MC JC | 4,060,444 | Δ19,761 bp | yihL–yiiF | 19 genes yihL, yihM, yihN, ompL, yihO, yihP, yihQ, yihR, yihS, yihT, yihU, yihV, csqR, yihX, yihY, dtd, fabY, yiiE, yiiF |
|
| MC JC | 4,086,996 | Δ13,531 bp | yiiG–rhaT | yiiG, frvR, frvX, frvB, frvA, rhaM, rhaD, rhaA, rhaB, rhaS, rhaR, rhaT | |
| RA | 4,139,288 | C→T | D141N (GAC→AAC) | fsaB ← | fructose‑6‑phosphate aldolase 2 |
| RA | 4,139,511 | Δ1 bp | coding (198/663 nt) | fsaB ← | fructose‑6‑phosphate aldolase 2 |
| MC JC | 4,139,771 | 10483 bp→TG | [ptsA]–[eptC] | [ptsA], frwC, frwB, pflD, pflC, frwD, yijO, [eptC] | |
| RA | 4,235,651 | T→C | intergenic (+244/‑255) | pgi → / → yjbE | glucose‑6‑phosphate isomerase/uncharacterized protein YjbE |
| MC JC | 4,235,905 | 16376 bp→52 bp | yjbE–malM | yjbE, yjbF, yjbG, yjbH, yjbT, psiE, xylE, malH, malG, malF, malE, malK, lamB, malM | |
| RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| MC JC | 4,299,522 | Δ12,535 bp | mdtP–alsB | mdtP, mdtO, mdtN, ytcA, yjcS, alsK, alsE, alsC, alsA, alsB | |
| RA | 4,312,070 | Δ1 bp | intergenic (‑28/+31) | alsB ← / ← alsR | D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,151 | G→C | Q281E (CAA→GAA) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,215 | A→G | T259T (ACT→ACC) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,219 | A→G | L258P (CTA→CCA) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,289 | A→G | Y235H (TAT→CAT) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,498 | C→G | R165P (CGC→CCC) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,313,027 | T→G | intergenic (‑36/‑323) | alsR ← / → rpiB | DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B |
| MC JC | 4,313,864 | Δ16,405 bp | yjdP–rdcB | 18 genes yjdP, phnP, phnO, phnN, phnM, phnL, phnK, phnJ, phnI, phnH, phnG, phnF, phnD, phnC, yjdN, yjdM, rdcA, rdcB |
|
| RA | 4,558,907 | T→A | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 357845 | 357933 | 89 | 6 [5] | [5] 6 | cynR | DNA‑binding transcriptional dual regulator CynR |
| * | * | ÷ | NC_000913 | 1978495 | 1979270–1978503 | 9–776 | 21 [0] | [0] 20 | insB5–insA5 | insB5,insA5 |
| * | * | ÷ | NC_000913 | 2406788 | 2407179 | 392 | 39 [0] | [0] 27 | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA |
| * | * | ÷ | NC_000913 | 3423843–3424229 | 3424432–3424238 | 10–590 | 6 [5] | [5] 6 | [rrlD] | [rrlD] |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 1397238 | NA (NA) | 21 (0.680) | 20/490 | 0.5 | 100% | noncoding (1195/1195 nt) | IS5 | repeat region |
| ? | NC_000913 | = 1978494 | 0 (0.000) | intergenic (‑297/+24) | flhD/insB5 | DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB | |||||
| * | ? | NC_000913 | 1978503 = | NA (NA) | 39 (1.270) | 37/488 | 0.0 | 100% | noncoding (768/768 nt) | IS1 | repeat region |
| ? | NC_000913 | = 2406787 | 0 (0.000) | intergenic (‑146/‑774) | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA | |||||
| * | ? | NC_000913 | 1979271 = | 0 (0.000) | 20 (0.650) | 18/490 | 0.6 | 100% | intergenic (‑56/‑482) | insA5/uspC | IS1 family protein InsA/universal stress protein C |
| ? | NC_000913 | = 2102947 | NA (NA) | pseudogene (443/446 nt) | wbbL | interrupted rhamnosyltransferase WbbL | |||||
| * | ? | NC_000913 | 2407180 = | 0 (0.000) | 27 (0.880) | 25/488 | 0.3 | 100% | intergenic (‑539/‑381) | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA |
| ? | NC_000913 | 3583428 = | NA (NA) | noncoding (1/768 nt) | IS1 | repeat region | |||||
| * | ? | NC_000913 | 4542682 = | 19 (0.620) | 7 (0.240) | 7/472 | 2.0 | 26.3% | intergenic (+49/‑433) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
| ? | NC_000913 | 4542996 = | 21 (0.710) | intergenic (+363/‑119) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit | |||||