Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,707,484	+GA :: IS3 (+) +4 bp	coding (219‑222/1608 nt)	dppA ←	dipeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	31 (1.010) +TC	29/486	0.1	100%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	= 3707487	0 (0.000)					coding (219/1608 nt)	dppA	dipeptide ABC transporter periplasmic binding protein
*	?	NC_000913	= 2171428	NA (NA)	25 (0.810)	22/490	0.4	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	3707484 =	0 (0.000)					coding (222/1608 nt)	dppA	dipeptide ABC transporter periplasmic binding protein

GAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2171185‑2171428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTAGCCTGAACACTTGCTGCGACGGTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGT  >  NC_000913/3707484‑3707727                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          GAGCGCAAAAGGTTGCTGCTACGATAACGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCCGTCCGGAACAATTTGAAAACAAGAACCTAGGTTAGGCCTGTGTCCATATTAAGGGGGGAGGAACACACTTCG                                                                                                                                                                                                                                               >  2:235324/1‑251    CGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTG                                                                                                                                                                                                                                            <  1:246483/251‑1      CAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGT                                                                                                                                                                                                                                          <  1:228964/251‑1      CAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCGGAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGT                                                                                                                                                                                                                                          <  1:148956/251‑1          AGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCCGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACCCTTCGGTGGTTCCG                                                                                                                                                                                                                                      >  2:113580/1‑251                     ACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCACTCCGGCACAATTTGAAAAACAGAAACTCGCTAAGGACTGGGGCCACATTACGTGGGGAAGATCACACTTCGGTGGggctgcatttaaattt                                                                                                                                                                                                                           >  2:204783/1‑235                                                 TCATTCGCTGAAAGTGTAATGTATCCATGGAGAACACTTTATCAGCCGGGAAAGAAGGCGGGCAACGGTGTTTAATGATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGA                                                                                                                                                                                                <  1:303629/250‑1                                                                   ATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAAAGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGAGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACACTTTGAAAACAAGAACCTTGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATACAGCGGGACGGAAGAGGCGTCATAGGTGGTG                                                                                                                                                                             >  1:177408/1‑251                                                                       ATCCATGGAGAACACTTAATCAGCAGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGCGATTACAATCGGTGGCGGCGGCACAGTTGGAGTGGAGGACTCAGTCCGGAAAAATTTGAAAACAAGAACCTCGCTTAGGACTGTGTCCATATTACGTGGGACGGATCCCACTTCGGAGGTGCCGATTTTAAATTCAACCAGACGGTTTTAAAGCGGGACGGAAGAGGCGTCATAGGAGGTGGCGG                                                                                                                                                                         >  2:283216/1‑251                                                                                       gaATCAGCCGGGAAATAAGGCGGGCAACGGTGTTTAAGTATATCGAATGTGATTACAATCGGTGGCTGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCGCGCTTAGGCCTGTGTGCATATTGCGTGGGTAGGATCACACTGCGGGGGTTCCGATGGTAAATTCAACCAGACGGTTATAATGCGGGAGGGAATAGGCGTCAGAGGTGGTGCCTGAGTTAAGCAGCTGC                                                                                                                                                           <  2:196316/247‑1                                                                                       TTATCAGCCAGGAAAGAATGCGGGCAACTGTGTTTAAGTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCTGCCTCAGTCCGGAACAATTTTAAAACAAGAACCTCGCTTAGGCCTGTTTCCATATTACGTGGGTAGGATCACACTTCGGGGGTGCCGAGTTTAAATTCAACCATACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTTCCGGAGGCAAACAGCTGC                                                                                                                                                           <  2:290439/249‑1                                                                                                           CGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGAGGCGGCGGCACAGTTGGTGTGGCGGCCTCCGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGG                                                                                                                                          >  1:36083/1‑246                                                                                                              GCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTT                                                                                                                                  <  2:220456/251‑1                                                                                                                   GGTGGTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAG                                                                                                                             <  2:110095/251‑1                                                                                                                        TTAATTATATCGAATGTGATTACAAGCGTTGGCGGCGGCACAGTTGGTGTGGCGGCCGCAGTCCGGAACAAGTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACATGTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGATGGAAGGGGCGTCATAGGTGGTGCCGGAGGTAATCAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAAT                                                                                                                         <  1:144504/250‑1                                                                                                                          AATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAA                                                                                                                       <  1:271216/250‑1                                                                                                                                    AATGTGATTACACTCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTT                                                                                                            >  1:217800/1‑251                                                                                                                                            TACAATCGGTGGCGGCGGCACAGTTGTGGTGGGGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACGTCGGTGGTGCCGATTTTAAATTCAACCAGACGGGTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTAGCCTGA                                                                                                     <  2:209166/250‑1                                                                                                                                            TACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTAGCCTGA                                                                                                     <  2:36083/250‑1                                                                                                                                                             GCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTAGCCTGAACACTTGCTGCGACGGTC                                                                                   <  1:235324/251‑1                                                                                                                                                                                                      ACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTATCCTGAACACTTGCTGCGACGGTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTT                                          <  1:183758/251‑1                                                                                                                                                                                                                              TCCATATTACGTGGGTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTAGCCTGAACACTTGCTGCGACGGTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCATGGAAATACGCATTATTCT                  >  2:217237/1‑251                                                                                                                                                                                                                                          GGGTAGTATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACATCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTAGCCTGAACTCTTGCTGCGACGGTCATAGCCACCAGGCGGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTG      <  2:185905/251‑1                                                                                                                                                                                                                                            GTAGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTAGCCTGAACACTTGCTGCGACGGTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGAT    <  1:113580/251‑1                                                                                                                                                                                                                                              AGGATCACACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTAGCCTGAACACTTGCTGCGACGGTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGT  <  1:283216/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          GAGCGCAAAAGGTTGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAATGTATCCATGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2171185‑2171428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACTTCGGTGGTGCCGATTTTAAATTCAACCAGACGGTTATAAAGCGGGACGGAAGAGGCGTCATAGGTGGTGCCGGAGGTAAACAGCTGCGGGTTAAACCCTTCCGGAGATCCTTCTGAGCAATAAACCAGAGTTTTAGCCTGAACACTTGCTGCGACGGTCATAGCCACCAGGCTGAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGT  >  NC_000913/3707484‑3707727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.