Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,498	C→G	R165P (CGC→CCC)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,498	0	C	G	100.0%	72.5 / NA	23	R165P (CGC→CCC)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (13/10);  total (13/10)

GCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAAT  >  NC_000913/4312278‑4312735                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              gcaaacaacatgatCGACCACTTTCGCTATCGCTGAATAGAAGCTCAGCGGTATCCAAATAATCTTTGCCACGTTCTTTTTTACACGTTCTACGGCCGCTTTTACCTCCCTGGTTCGCCCGGAATCGGTCCCTAACAGCACCACATCTTCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACCTGTGCTGTACATCAgacc                                                                                                                                                                                                                    >  2:284487/13‑245 (MQ=255)       aatatgatCGGCCAGTTTCGCTATCGGTGTATGGTAGCTATGGGTTATACAAATAATCTTTGCACCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTTCATCCCTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCATTAACTTGGTCTGTACTTCAGCACAGATAGCAt                                                                                                                                                                                                           <  2:186990/251‑1 (MQ=255)                         cGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCAGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGt                                                                                                                                                                                         >  2:154483/1‑251 (MQ=255)                          gCTATCGGTGGATGGTAGCTATGGGTTATACAAGTAATCTTTGCACCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGCTGTGAGCATCAGGCTAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCCCAGCTAGCATTTGATCCTCCGGccacgt                                                                                                                                                                                         >  2:140408/1‑245 (MQ=255)                                     aTGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTGTGCCAGTTCTACGGCCGCTTTTACAGCACTGGTTCGCCCGGAATGGGTCACGACCAGCACAACATCTCCTGCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCAGCAGGATAGGCCTGACATCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCAGTTGATCCTCCGGCACCGTACAAAGCCCGCt                                                                                                                                                                             <  2:261513/251‑1 (MQ=255)                                                 ggTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGAt                                                                                                                                                                 <  1:98493/251‑1 (MQ=255)                                                   ttATACACATAATCTTTGCCCCGTTCTTTTTTGCCAGATCTCCGGCAGCTTTTACATCAATGGTTCGCCCGGACTGGGTCACTACCACCACAACATCTCCTTCCTGTCACAACGAAGCGGACATCATCATGATGTGAGCATCCGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGAt                                                                                                                                                                 >  2:273960/1‑249 (MQ=255)                                                       aCAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGGTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGaaa                                                                                                                                                            <  2:107264/250‑1 (MQ=255)                                                                cTTTGCCCCGTTCTTTTTTGCCAGTTATACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACCTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGCTAGAAAAAgcgggcgg                                                                                                                                                  >  2:203075/1‑251 (MQ=255)                                                                       ccGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTg                                                                                                                                           >  1:37907/1‑251 (MQ=255)                                                                           tCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCTCCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGAt                                                                                                                                        <  1:154483/250‑1 (MQ=255)                                                                               ttttGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATCGAAAAAGCGGGCGGCACGGTGGATCtcat                                                                                                                                   >  2:297035/1‑251 (MQ=255)                                                                                                         cATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGCTCTCATCAACGTTGACGATCGACTgcacttcc                                                                                                         >  2:36250/1‑244 (MQ=255)                                                                                                                        cGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTaa                                                                                           >  2:12311/1‑250 (MQ=255)                                                                                                                        cGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTaa                                                                                           >  1:225267/1‑250 (MQ=255)                                                                                                                         ggAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATATGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCGCCGGCACCTTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTTGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAg                                                                                         <  1:140408/251‑1 (MQ=255)                                                                                                                              gggTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGGTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGAt                                                                                     <  1:297035/250‑1 (MQ=255)                                                                                                                                         aGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCtt                                                                         >  1:240696/1‑251 (MQ=255)                                                                                                                                             caacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTc                                                                     <  1:256177/251‑1 (MQ=255)                                                                                                                                                              aacaacGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGgcgc                                                    >  2:85394/1‑251 (MQ=255)                                                                                                                                                               acaacGAAGCGGACCTCATCATGATGTGAGCATCAGGATAGGACTGACAGCGTCCGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCATGCGGCGCt                                                   >  2:274648/1‑251 (MQ=255)                                                                                                                                                                                                 cggatcagCCTGACAGCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGGACAGATAGCATTTGACCCTCCGGCACCGTACAAATTCCGGTGTCGGGCCTGATAGAAAAAGCGGGCGGCGCGGTGGATCTCATCAACGTTGACGATCGACTGAACTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCTCATCCTGCGGCGCTTCAGCAAAAGCCAGCACGGAAGGCGATACCTGtt                 <  2:167722/244‑1 (MQ=255)                                                                                                                                                                                                                gCGTACGCCAATGGGCAAGAACTTGTGCTGTACATCAGCACATATAGCATTTGATCCGCCGGCACCGTACAAATCCCTCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAATTCGGAAGGCAATACCTGTTCTGACTGAGAAAaat  <  2:67313/251‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              GCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCCTGATAGAAAAAGCGGGCGGCACGGTGGATCTCATCAACGTTGACGATCGACTGACCTTCCATAATGGTGCGTAAAGTGATGTTAAATACCTTATTCACCACATCCTGCGGCGCTTCATCAAAAGCCAACTCGGAAGGCAATACCTGTTCTGACTGAGAAAAAT  >  NC_000913/4312278‑4312735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑