Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,173	T→C	L155P (CTC→CCC)	hyi →	hydroxypyruvate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,173	0	T	C	100.0%	92.2 / NA	28	L155P (CTC→CCC)	hyi	hydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/15);  total (13/15)

TCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGG  >  NC_000913/535937‑536418                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      tCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGc                                                                                                                                                                                                                                         >  1:161252/1‑251 (MQ=255)                   gTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAattgattg                                                                                                                                                                                                                       <  1:174337/251‑1 (MQ=255)                         ggATGGCGTAGCAGCAGCGATTAGTTATGCCCGTGCGCTGAGAACTAAAAAAATTAACTGACTGGTCGATAAAACGCCGGATGGTTTCCGCAGTGAACAGCTCCACGAAACGCTTGTAGAAAACCTGAGTTATGCCGCGAATATGCTGCTGACAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATtgatgctg                                                                                                                                                                                                                 >  1:197664/1‑248 (MQ=255)                          gATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgt                                                                                                                                                                                                                >  1:72087/1‑251 (MQ=255)                                           gATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTg                                                                                                                                                                                                >  2:95747/1‑250 (MQ=255)                                              tCGTTATGCCCGTGCGCTGGGTACTAACAACATTAAATGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGCACAGATTCACGCAACGCTTGTAGCAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGACGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCAATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGaaa                                                                                                                                                                                             >  2:196162/1‑250 (MQ=255)                                                   aTGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGTCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCa                                                                                                                                                                                        <  1:190970/250‑1 (MQ=255)                                                         gTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGAc                                                                                                                                                                                 <  1:8422/251‑1 (MQ=255)                                                             gcTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTGACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACAttt                                                                                                                                                                             <  2:11601/251‑1 (MQ=255)                                                                      taaAAAAATTAACTGACTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGATTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAACGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGc                                                                                                                                                                    >  2:153395/1‑251 (MQ=255)                                                                             aTTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTCGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGAt                                                                                                                                                             <  2:219384/251‑1 (MQ=255)                                                                                            ggTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTTAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAAc                                                                                                                                              <  2:290565/251‑1 (MQ=255)                                                                                            ggTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTGGGTTGCTGCAAGTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAAc                                                                                                                                              <  1:153395/251‑1 (MQ=255)                                                                                            ggTAAAAAGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGATTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGTTGGAAGGTGAATTAAc                                                                                                                                              >  2:275124/1‑251 (MQ=255)                                                                                                       ggCTGGTTTCCCCAGTGAACAGCTTCACGCAACGCTTGTAGAAGACCTGCGTTATGCCGCGAATATGATGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAAACATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGAGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATg                                                                                                                                    >  2:34825/1‑250 (MQ=255)                                                                                                            gTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTc                                                                                                                                >  1:160383/1‑249 (MQ=255)                                                                                                                     gTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGAt                                                                                                                     <  1:95747/251‑1 (MQ=255)                                                                                                                                                 aaaCCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATa                                                                                         <  1:172529/251‑1 (MQ=255)                                                                                                                                                                cgcgAATATGCTGATGAAAGAAGATATTTTATTACTTATTGAACCTATTAACCATTTTGATATACCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCGCCTGCAAATTGCCGATAATCCGCATCgcggcg                                                                          <  2:76928/251‑1 (MQ=255)                                                                                                                                                                   gAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACt                                                                                                                                                                                                                                             <  2:117152/85‑1 (MQ=255)                                                                                                                                                                   gAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACt                                                                                                                                                                                                                                             >  1:117152/1‑85 (MQ=255)                                                                                                                                                                            gatgaAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaac                                                                <  2:72087/249‑1 (MQ=255)                                                                                                                                                                             atgaAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCgaaccgggaaccgg                                                             <  2:161252/251‑1 (MQ=255)                                                                                                                                                                                 aagaagATATTTTATTAGTGATTGAACCTATTACCCCTTTTGATATTCCTGGTTTCCATCCCACCGGAACTCTGCAGGCGCTGAAATTGCTTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGCATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCCAATTGCCGATAATCCGCCTCGCGGCGAACCGGGAACCGgagc                                                          >  2:214070/1‑249 (MQ=255)                                                                                                                                                                                     agaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGCTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTCCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGACGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAaatt                                                      >  1:194678/1‑250 (MQ=255)                                                                                                                                                                                                                 aaCCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCACTGAAATTGATTGATGCTGTCGCTTGCTGCAATTTGAAACTTCAGTCTGACATTTAACCTATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCACATTGCCGATAATCCGCATCCCGGCGCACCGGGAACCGGAGAACTTAATTATGATTATCTCTTTCAGGCAATCg                          >  1:196010/1‑250 (MQ=255)                                                                                                                                                                                                                                       tCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACggttg    <  1:76621/250‑1 (MQ=255)                                                                                                                                                                                                                                        ccATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACggttggg  <  2:34214/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      TCCTGGCCGTGAAGAAGAGTTTCGGGATGGCGTAGCAGCAGCGATTCGTTATGCCCGTGCGCTGGGTAATAAAAAAATTAACTGTCTGGTCGGTAAAACGCCGGCTGGTTTCAGCAGTGAACAGATTCACGCAACGCTTGTAGAAAACCTGCGTTATGCCGCGAATATGCTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGACATTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGG  >  NC_000913/535937‑536418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑