Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,492	A→G	intergenic (+6/‑94)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,492	0	A	G	100.0%	72.3 / NA	25	intergenic (+6/‑94)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/14);  total (11/14)

TTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCC  >  NC_000913/536246‑536736                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     ttATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGAGGGCTGATAAAATTGGTCACCCGCAAATTGCCGATAATCCGCATCGCGGCGAAACGGGACCCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGCTCCGTACCGTTAAAACGTGACGc                                                                                                                                                                                                                                                  >  1:153052/1‑251 (MQ=255)                       ggTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAg                                                                                                                                                                                                                            <  1:60410/251‑1 (MQ=255)                          gaagtAACCAACACCATGACTCATTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCTCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCGCGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGc                                                                                                                                                                                                                          <  1:196162/246‑1 (MQ=255)                                                aGTGGGCTGATAAAATTGGTCAACTCCCAATTGCCGCTCATCAGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTACGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGACGGTTTACGCTGGATGGATCCGTACCGTTACAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGATAAGTTGGCAGGGGATCgtg                                                                                                                                                                                                   >  2:284276/1‑251 (MQ=255)                                                        gATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGGTTACGCTGGATGGAGCCGTACCGTTAAAACGTGACGCGATTCAGACAATGCGTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTcc                                                                                                                                                                                             <  1:34825/249‑2 (MQ=255)                                                                         gCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGaa                                                                                                                                                                           >  1:7477/1‑250 (MQ=255)                                                                                 ccGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAAGATAAACCCCAAACCACCACGGAAGCCGGTTTACGCGGGATGGATCCGTACCGTTAAAACGTGGCGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGTAAAAGCGAAATTTAAAag                                                                                                                                                                  <  2:40329/251‑1 (MQ=255)                                                                                            cATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTAt                                                                                                                                                       >  1:76485/1‑251 (MQ=255)                                                                                            cATCGCGGCGAACCAGGAACCGTAAAAATTAATTATGTTTATCTCTTTAAGGTAATCGAAAATTCTGACTTCAACGGTTGGGTTGGGGGTAAATATAAGCCCCAAACCGCCACGGAAGCCTGTTTCCGCTTGATGGATCCGTACCGTTAAAACGCGACTGTATTCAGACAATGCTTTTTGAGGCCGCTAAGTTGGCAGGGGATCGTGTTGGCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTAt                                                                                                                                                       <  1:189189/251‑1 (MQ=255)                                                                                                  ggcgAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACt                                                                                                                                                 <  1:275124/251‑1 (MQ=255)                                                                                                            gaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGGGCCGTTAAAACGGGACGCTATTCAGGCAATGCTTTTTGATGCCGCTAAGTTTGCATGGGGTCGTGTTGTCCTAATTCGGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTAtt                                                                                                                                       <  1:24013/251‑1 (MQ=255)                                                                                                                                gATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcg                                                                                                                   <  1:214070‑M1/251‑8 (MQ=255)                                                                                                                                          ttAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTGTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGGGGTTAATTATAAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcat                                                                                                            <  1:284276‑M1/248‑15 (MQ=255)                                                                                                                                                      aaaaGTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcct                                                                                             <  2:160383‑M1/251‑30 (MQ=255)                                                                                                                                                           tCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTCGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgc                                                                                        >  2:301271‑M1/1‑217 (MQ=255)                                                                                                                                                               aCTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAACCGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacg                                                                                    >  2:69120‑M1/1‑213 (MQ=255)                                                                                                                                                               aCTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacg                                                                                    >  1:88541‑M1/1‑213 (MQ=255)                                                                                                                                                                      cGGTTGGTGTGGGTGTGAATATAAACCCCAAACCACCCCGGCAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAATCGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAATTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGATGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtggtcgtaggccttagacatttgttcctgatgcgacgcttgccg                                                                             <  2:173504‑M1/251‑46 (MQ=255)                                                                                                                                                                                gggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTACCACGTGACGCTATTCAGACAATGCTTCTTTAGGCCGCTACGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagccatttgtgcctgatgcgacgcttgacgcgtcttatc                                                                    >  1:136345‑M1/1‑196 (MQ=255)                                                                                                                                                                                                cccAAACCACCACGGAAGCCGGTTTACGCTGGATGGATACGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAGGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCCTTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgc                                                   >  2:281840‑M1/1‑180 (MQ=255)                                                                                                                                                                                                      ccaccaCGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatc                                             >  2:136406‑M1/1‑174 (MQ=255)                                                                                                                                                                                                        accacGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcatacaaccggtgccgcatccg                                           <  1:87895‑M1/251‑80 (MQ=255)                                                                                                                                                                                                                                        gTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgctt           <  2:88541‑M1/251‑112 (MQ=255)                                                                                                                                                                                                                                          aCCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttg          <  1:95267‑M1/250‑113 (MQ=255)                                                                                                                                                                                                                                                 aaaCGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatct  >  1:244768‑M1/1‑131 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCC  >  NC_000913/536246‑536736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑