Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	155,463	A→T	*247K (TAA→AAA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	155,463	0	A	T	100.0%	94.9 / NA	28	*247K (TAA→AAA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/15);  total (13/15)

AAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACC  >  NC_000913/155222‑155705                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    aaaCTCTGACGTCGTAAACCCCCTGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCGATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg                                                                                                                                                                                                                                             <  2:256552/250‑1 (MQ=255)                   cacCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTCCCCCATCAGGAAGGAATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGcc                                                                                                                                                                                                                          >  1:190409/1‑251 (MQ=255)                      ccGGTATAGCGGGGTTACATTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacc                                                                                                                                                                                                                       >  2:215468/1‑251 (MQ=255)                       cGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca                                                                                                                                                                                                                      <  1:10712/251‑1 (MQ=255)                                      aCCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCg                                                                                                                                                                                                       >  1:239942/1‑251 (MQ=255)                                               gTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTAGATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGc                                                                                                                                                                                              <  1:106384/251‑1 (MQ=255)                                                  ccGGCTGAGATCAATATTAGATGCATTCTGCCCCATATGGAAGGTATGGCCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTCGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACACTATGCTTACTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACt                                                                                                                                                                                           >  1:18806/1‑251 (MQ=255)                                                              aacagtcgagaccttcgtccccATCAGGAAGGTATGGTGATATTCAACGAGTGCATCACTGAAAGCGGAAGTGCAATCCAGCAGCGCGCAAAACGTGGCGAAACTGATCAGATCATGATAAGTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCCGAAGTATATCCTTGCCTTATTTCAGGCTGGCATTCCCTTCAACTGAGCCACCAAAGCCATTAATGTCGTAAAAATCCACTCTT‑GCAGAATTc                                                                                                                                                                              <  2:14449/233‑1 (MQ=255)                                                                                  ccATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCAtt                                                                                                                                                          <  1:94062/251‑1 (MQ=255)                                                                                           aGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGc                                                                                                                                                                                                        <  1:217949/197‑1 (MQ=255)                                                                                           aGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGc                                                                                                                                                                                                        >  2:217949/1‑197 (MQ=255)                                                                                                       atTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACt                                                                                                                                     <  2:239942/251‑1 (MQ=255)                                                                                                                     gCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCa                                                                                                                       <  2:90653/251‑1 (MQ=255)                                                                                                                          gAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAg                                                                                                                                                                >  1:47150/1‑205 (MQ=255)                                                                                                                          gAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAg                                                                                                                                                                <  2:47150/205‑1 (MQ=255)                                                                                                                          gAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACAAAATGGTGCAATCATTTTc                                                                                                                  <  2:51709/251‑1 (MQ=255)                                                                                                                               cAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATc                                                                                                             >  2:236602/1‑251 (MQ=255)                                                                                                                                      gCAATACAGCAGCGCGCAAAACGTGGCGATACGGGGCAGATGATGATAATTTGTAGTATATTCTATAGTCACGCTATGCTTCCCGCGGAATTATATCCTTGCCTGATCTCAGCCTGGCATTTCCTTCAAGTGCTCCACCAAAGTCATTAATGGCGCAAAAGTGCACTGTT‑GCATATTACGCGTTGCCATTAAGGCCAGTGCCTTTCACGTCTGCATCACCAAATCGTGCaaacgttgtcggatcgatcgg                                                                                                       <  2:131988/250‑20 (MQ=255)                                                                                                                                           acggcagcGCCCGAAACGTGGCGAGACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCAGGCTATGCTTCCTGCGGACTTATATCCTCGCCTGATTTCAGCATGGCATTACCTTCAACTGCGCCACCAAAGTCATTAATGGCGGAACACTG‑ACTTTTTGGAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACAAAATGGTGCCATCATTTACACGTGAATCGGTTAggg                                                                                                 >  2:166162/4‑249 (MQ=255)                                                                                                                                                          aCGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCTAAGTCATTAATGGCGTAAAAATGCTCTTTT‑GCAGAATTCGCCTTGCCATTAAGGCCATTGGCTTTCATGGCCTCATCACTTAATGGTGCAATCATTTTCACATCAATCGGATATCGTTTACCGCTAGCTTc                                                                                  <  1:272772/251‑1 (MQ=255)                                                                                                                                                                     cGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATcaccac                                                                       <  2:257992/251‑1 (MQ=255)                                                                                                                                                                         tCAGATGATGATACTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCt                                                                   >  2:135729/1‑251 (MQ=255)                                                                                                                                                                                      aTTTTTAGTATATTCTATAGTCACGCTATGATTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCACAGTCATTCATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACgt                                                        >  1:40294/1‑249 (MQ=255)                                                                                                                                                                                            aGTATATTCTATAGTCACGATATGCTTCCTGCGGAATTATCTCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAggg                                                >  1:259964/1‑251 (MQ=255)                                                                                                                                                                                               atatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGtt                                             <  2:218223/251‑1 (MQ=255)                                                                                                                                                                                                                                    atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTa                  >  2:110542/1‑241 (MQ=255)                                                                                                                                                                                                                                    atCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTa                  <  1:110542/241‑1 (MQ=255)                                                                                                                                                                                                                                            cTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGACATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATCACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGAcc  >  1:144617/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    AAACACTGACGTCGTAAACACCCGGTATAGCGGGGTTACCTTCACTGTACCGGCTGAGATCAATATTAGATGCATTCTGCCCCATCAGGAAGGTATGGTCATATTCAACGAGTTCAGCACTGAAAGCAGTATTGCAATACAGCAGCGCGCAAAACGTGGCGATACGGGTCAGATGATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTT‑GCAGAATTCGCTTTGCCATTAAGGCCATTGACTTTCATGACCTCATCACTAAATGGTGCAATCATTTTCACATCAATCGGATAGCGTTTACCGCTAGCTTCTAAATCACCACTGCTAAAAGAGACGTAGTAAGGGGTTGGATTGGTCACTCGTAATGACGCCTTACCTTCTGAACCTGACC  >  NC_000913/155222‑155705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑