Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	100.0%	72.3 / NA	22	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/6);  total (16/6)

ACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTG  >  NC_000913/3485959‑3486437                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         ccAGTAATCCATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTTCAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGCCAAGCCAGAGACAGCGGCGTTATCTGGCCCTGGAGAAAGCTTTTAACAGAGGATAACCGCGCATGGTCCTTGGCAACCCGCAAACACACCCGACTCTCGAATGGTTCATGGCTCATTGCCACATTCATAAGTTCCCATCTAAGAGCAAGCt                                                                                                                                                                                                                                       <  1:256141/249‑1 (MQ=255)      aaTACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTc                                                                                                                                                                                                                                  >  1:92641/1‑250 (MQ=255)                                          acaTTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGCAAGCTTATAACAGAGGCTAACCGCGCCTGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGCATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCCTCCAAGAGCAAGCTTATTCACCAGGGTGATACAGCGGAAACTCTGTACTACATCGt                                                                                                                                                                                             >  1:133026/1‑251 (MQ=255)                                                 cGTGAAGTACATTGGATAGCCCCTTCCCATGTAGCGGTAAGCATATTTCGGAAATCCAGAGACACCGGCGTTATCTGGCTCGGGAGAAAGCTTATAACAGAGGATACCCTCGCATGGTCCTTGGCAACCCGCAAACAGACCCGACTCTCGAATGGTTCTTTTCTCATTGCCACATTCAGAAGTACCCATCCAAGAGCAAGGTTATTCACCAGGGTGAAAAATCGGAAACGCTTTACGACATCGTGAAAGGc                                                                                                                                                                                      <  2:288350/251‑1 (MQ=255)                                                    gCAGTCCAGTTGATACCCCCTTCCCAGGTGGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGctct                                                                                                                                                                                   <  2:7531/251‑1 (MQ=255)                                                                         tCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGa                                                                                                                                                               <  1:6154/250‑1 (MQ=255)                                                                              ggTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa                                                                                                                                                            >  1:227143/1‑248 (MQ=255)                                                                                   cGGGAAGCATATTTCGGCAATCAAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCCAACAGACCCGACTCTCGAATGGTTCTTGTATCATTGCCACATTCATAAGTACCCATACAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACCTCGTTACAGGCTCTGTGGCAGTGCGGATCCAAGACGAAGAggtt                                                                                                                                                     >  2:174668/1‑248 (MQ=255)                                                                                             atTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAACGCGGAAACGCTGTACTACATCGTTCAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGAt                                                                                                                                          >  1:103316/1‑251 (MQ=255)                                                                                                                 gcggcgTTATCTTGCGCTGGAGAAAGCTTATAACAGAGGATAGCCGCGCATGGTGCTTGGCACACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCAGTGCCACATTCATAAGTACCCAGCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAgg                                                                                                                      <  2:220580/251‑1 (MQ=255)                                                                                                                      gTTATCTGGCTCTGGAGAAAGCTTATAAAAGAGGATAACCGCGCATGGGGCTTGGCAAACCGCAAACAGACCCGCCTCTCGAATGGTTCTTGTCTCATTGCCAAATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGATGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAt                                                                                                                 >  1:262797/1‑251 (MQ=255)                                                                                                                          tCTGGCTATGGCGATAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACACGACTCTAGAATGGTTCTTGTCTCATTGCCACATTAATAAGTCCCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCATCTCCTATCTGAATCAGGGTGAtttt                                                                                                              >  2:38300/1‑250 (MQ=255)                                                                                                                            tGGCTCTGGAGAAAGCTTATCACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTAtt                                                                                                           >  1:258379/1‑251 (MQ=255)                                                                                                                             ggCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGAACTTGGCACACCGCAAACAGACCCGCCTCTCGAATGGCTATTGTCTCATTGCCACATTCATAAGTACCCATCAAAGCGCAAGCTTATTCCCCAGGGGGAAACAGCGGAAACGCTGTACTACATCGTTAAAGGCACTCCGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGCTTTTATTg                                                                                                          >  1:266897/1‑251 (MQ=255)                                                                                                                                      gaAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTgg                                                                                                 >  1:121981/1‑251 (MQ=255)                                                                                                                                                       gATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGc                                                                                >  1:101846/1‑251 (MQ=255)                                                                                                                                                       gATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGAACCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAACTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGc                                                                                >  1:26501/1‑251 (MQ=255)                                                                                                                                                                           ggCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGt                                                            >  1:108009/1‑251 (MQ=255)                                                                                                                                                                                                  tctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGc                                                                                                                                                                                                          <  1:90574/86‑1 (MQ=255)                                                                                                                                                                                                  tctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGc                                                                                                                                                                                                          >  2:90574/1‑86 (MQ=255)                                                                                                                                                                                                          ggTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGa                              >  1:294436/1‑250 (MQ=255)                                                                                                                                                                                                                                      gTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTg  >  1:170616/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         ACAGTAATACATTGATGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTG  >  NC_000913/3485959‑3486437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑