Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,313,027	T→G	intergenic (‑36/‑323)	alsR ← / → rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,313,027	0	T	G	100.0%	58.8 / NA	18	intergenic (‑36/‑323)	alsR/rpiB	DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/9);  total (9/9)

GCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCAT  >  NC_000913/4312807‑4313272                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      gCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTg                                                                                                                                                                                                                         >  1:166083/1‑251 (MQ=255)       aaTACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCGCCACAGCAAGAACAGAAAAGATGCTGGCGACt                                                                                                                                                                                                                   <  2:225267/250‑1 (MQ=255)                      tCTGCGACATCGTTAATTGGGGGTGCACAACTTAGGTTACCGGGTTTGAGTAACCACTCCACGGTGCGGGTTTCATTTTCTGTAAGTCCTTCCTGCAGCAGTCGCAGGGAAGGCGCTAACCCTAGACCGTTCGGAGGCGCTGAATCAAACTCTGACGCGCTCATCGCGGTCTCTTGGTCTTGAAGAGAAGATGCCAGTAGAGCACCACAGCAAGAACAGTAAAGTTGATTGCGTCTTTACAAAaaaataa                                                                                                                                                                                                     <  2:132644/250‑1 (MQ=255)                          cGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAAttt                                                                                                                                                                                                >  1:1741/1‑251 (MQ=255)                                           ggTGCACAACTCAGGTTACCGGGTTTGAGGAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTttattttatt                                                                                                                                                                               <  1:36250/251‑1 (MQ=255)                                                                    tGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTaaa                                                                                                                                                       <  2:14558/250‑1 (MQ=255)                                                                                    gATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAAt                                                                                                                                      >  1:261713/1‑251 (MQ=255)                                                                                     aTGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGGAAGGCGCTAACCCTATGCCTTTCGGATGCGCTGAATCAAACTCTGACGGGGTCAGCGCTGTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAAGAAAATTTATAAATTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAAtt                                                                                                                                     <  1:284487/251‑1 (MQ=255)                                                                                                           ttccttccTGCTTCATTCGCAGGTAAGGCGCTACCCCTATACCGTTCGGCAGCGCTGAATCCAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGACAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTAtt                                                                                                               >  2:248988/1‑251 (MQ=255)                                                                                                             ccttcctGCTTCAATCGCAGGTAATGCGCTACACCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATTCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGGTAttt                                                                                                              <  2:145987/250‑1 (MQ=255)                                                                                                                    gCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCGCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACGTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGGGGCTTAAAATTTTATTGCATTTAATTAGTTTAAAAACATGAGGTGATTTGTTTTTc                                                                                                       <  2:37907/250‑1 (MQ=255)                                                                                                                                ggTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAgat                                                                                          <  1:203075/251‑1 (MQ=255)                                                                                                                                               cTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTCCTCTTGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACATCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTgg                                                                           >  1:194848/1‑251 (MQ=255)                                                                                                                                                                                          caCGTTCTCTTGTCCTTGAAGTGAAGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAAct                                 <  1:248988/250‑1 (MQ=255)                                                                                                                                                                                                   ttGTCCTTGAATTGAAGACGCCACTAGATCACCACAGCGAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAAc                        >  1:221381/1‑250 (MQ=255)                                                                                                                                                                                                                 aaGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGt         >  1:63146/1‑251 (MQ=255)                                                                                                                                                                                                                  aGACGCCACTAGATCACCACAGCAAGAACAGAAAAGATCCTGGCGCCTTCACAAAAAAATAACATTTATACAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTAATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGt         >  1:122223/1‑250 (MQ=255)                                                                                                                                                                                                                        cACTAGATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCAt  >  1:264130/1‑251 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      GCTTCAGATACCGCCAGAGCTTCTGCGACATCTTTAATTGCGGGTGCACAACTCAGGTTACCGGGTTTGAGTAACCACTCCACGATGCGGCTTTCATTTTCTGTCATTCCTTCCTGCTTCATTCGCAGGTAAGGCGCTAACCCTATACCGTTCGGAAGCGCTGAATCAAACTCTGACTGGCTCATCACGTTCTCTTGTCCTTGAATTGAAGACGCCACTATATCACCACAGCAAGAACAGAAAAGATGCTGGCGACTTCACAAAAAAATAAAATTTATAAAGTTTATTTTATTGTGAACAATGGCGAGTGGCTTAAAATTTTATTTCATTTAATTAGTTTAAAAACATGATGTTATTTGTTTTTCTGGTTTTTTAGATGATAGATGTTGCTGGATGGTGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCAT  >  NC_000913/4312807‑4313272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑