Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,105,376	IS2 (–) +5 bp	intergenic (+83/‑34)	csgC → / → ymdA	curlin chaperone/uncharacterized protein YmdA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	381260 =	NA (NA)	28 (0.910)	26/488	0.2	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	1105376 =	0 (0.000)					intergenic (+83/‑38)	csgC/ymdA	curlin chaperone/uncharacterized protein YmdA
*	?	NC_000913	= 1105380	0 (0.000)	28 (0.910)	24/490	0.3	100%	intergenic (+87/‑34)	csgC/ymdA	curlin chaperone/uncharacterized protein YmdA
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/381479‑381260 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACT  >  NC_000913/1105376‑1105612                                                                                                                                                                                                                                                                                                                                                                                                                                                                           GGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAA                                                                                                                                                                                                                  <  1:162264/249‑1        ACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAA                                                                                                                                                                                                         <  1:216544/251‑1             ATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTT                                                                                                                                                                                                    <  2:241978/251‑1                            GAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCCTTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAA                                                                                                                                                                                      >  2:253441/1‑250                                GAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCT                                                                                                                                                                                 <  2:126578/251‑1                                      ggCATCCCCGGTTCAAAGCTCTGCTGAACAATTGAGATCTTTTCCTGTGTGGCACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGCCTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATCTTCAGACCTTTTTTAAACTCTCTTATG                                                                                                                                                                           >  2:144369/3‑251                                                          cgGCTGAACAATTGCGATCTTTTCCTGGGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTTAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTC                                                                                                                                                        <  1:278823/248‑1                                                                   AATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTATCCGGACCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGC                                                                                                                                              >  1:122474/1‑251                                                                              TTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCT                                                                                                                                       <  1:253441/247‑1                                                                              TTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCT                                                                                                                                       <  2:45555/247‑1                                                                                           ACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCCTCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTCAACTCTCTTCTGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGG                                                                                                                      >  1:116831/1‑251                                                                                           ACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCGTATGCTCGGCAGTTTGTTTGTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAG                                                                                                                       <  2:82070/250‑1                                                                                                                            AATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGG                                                                                      >  1:61224/1‑250                                                                                                                                ATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTAGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCG                                                                                 >  1:35499/1‑251                                                                                                                                          CAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACG                                                                       >  2:215930/1‑251                                                                                                                                              GACTAGTCTAAATACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCAGTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCGCTTATGCTCGGCATTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTTATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCA                                                                    <  1:175193/250‑1                                                                                                                                                     CTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGCTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGCCGTCAGCACCC                                                              >  2:239484/1‑249                                                                                                                                                              AGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTGTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGGCATCACC                                                    <  2:180699/250‑1                                                                                                                                                                 ATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCGTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTA                                                <  2:53521/251‑1                                                                                                                                                                         TATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGA                                         >  2:18909/1‑250                                                                                                                                                                                 ATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCAC                                >  1:300658/1‑251                                                                                                                                                                                         GATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACATTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGT                        >  1:144237/1‑251                                                                                                                                                                                            ATTGGTTGTCGGGAGATGCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTTGAAGCACCGTGCAAGGGGGCGTGATCCATTTGTATGGCCAAATTGTGGAACCGGCATTTCACGTCAGCACCCGGTCATCACCCGTAGAAATGATCTGCACACAGAATGGTTCT                     <  2:177896/251‑1                                                                                                                                                                                                GTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCGCTTATGCTCTGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTTTGGAACCGGCAAGTGACGTCAGCACCCAGTCAGCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTC                 <  1:188535/251‑1                                                                                                                                                                                                   GTCTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGACGACTAATAAATCAGGAAATAGAAAACATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCACACAGAATGGTTCTATTCagg              >  1:23382/1‑248                                                                                                                                                                                                     CTGGAGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCCCCCGTAGAAATGAACTGCCCACCGAATGGTTCTATTCCGGGC            >  2:83411/1‑251                                                                                                                                                                                                         AGATTCAGGGGGCCAGTCTAACCCTCAAGGATGACTCATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGCAGAAATGAACTGCCCACAGAATGGGTCTCTTCCGGGCAAA         >  2:6793/1‑250                                                                                                                                                                                                               AGGGGGCCAGTCTAACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACT  <  1:215930/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                           GGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/381479‑381260 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aACCCTCAAGGATGACTAATCATTGAGGAAATAGAATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACT  >  NC_000913/1105376‑1105612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.