Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,139,288	C→T	D141N (GAC→AAC)	fsaB ←	fructose‑6‑phosphate aldolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,139,288	0	C	T	97.4%	118.9 / NA	38	D141N (GAC→AAC)	fsaB	fructose‑6‑phosphate aldolase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (19/18);  minor base G (0/1);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTC  >  NC_000913/4139043‑4139526                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               ccTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGc                                                                                                                                                                                                                                           >  2:108651/1‑251 (MQ=255)        agaTGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGATGCGCTACATCTAAGGGCAGGGTGATGGATTGACATCCTGCCAGTACACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTTTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGc                                                                                                                                                                                                                                    >  1:267977/1‑251 (MQ=255)        agaTGAGAAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGTCAGGGAGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAACCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTCCTAACAGCGTTTGCAGCTCCTGAACCGGACGAATGCCGTTTCACCCCTGGGc                                                                                                                                                                                                                                    >  2:170456/1‑251 (MQ=255)         gaTGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGc                                                                                                                                                                                                                                    >  2:38303/1‑250 (MQ=255)          aTGAGTAGTGCCAAATGCGGCTTTCCGGTCGTTTTCAAACTTCTCTATAGCTGATCCTACCGCAGGGGTGTTGGGCATTTGTTGCGCTACATCTAAGGGCAGGGTAATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGTCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTGCCGCCCTGGGCAt                                                                                                                                                                                                                                  <  2:203129/251‑1 (MQ=255)              gtagtGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCACTATAGCTGCCTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTCCATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTAc                                                                                                                                                                                                                              >  1:300991/1‑251 (MQ=255)              gtagtGCCAAATGCGGCATTCCAGGCGTGTTCGACCTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGGGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTAc                                                                                                                                                                                                                              <  1:24127/251‑1 (MQ=255)                agtGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGCGATGGCTTCACATCCTGCCAGTCAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTAcgc                                                                                                                                                                                                                            >  1:286976/1‑251 (MQ=255)                                 tCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGc                                                                                                                                                                                                            >  2:2254/1‑250 (MQ=255)                                               aaCTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAACCAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACCGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCCTCTACGCGGTTCACATACGGAGCAACGTATTTTGcccc                                                                                                                                                                                             >  2:1201/1‑251 (MQ=255)                                                      ctATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGcc                                                                                                                                                                                         <  1:108651/248‑1 (MQ=255)                                                                    cccGCAGGGGTGTGTAGCATTTGCTGCCCAACATCTAAGGGCAGGGTGAGGAATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTTCCAGCACCATGCTTTCGGGCGCGGGCATTTCTAACAGCGGTTGCAGCTCCAGAACCGTACAAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTGGCCCCTGCCAGGGCTGCTAATAcccc                                                                                                                                                                        <  2:267977/250‑1 (MQ=255)                                                                    aCCGCAGGGGTGTTGAGCATTTATTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAAcc                                                                                                                                                                         <  1:59167/250‑1 (MQ=255)                                                                         aGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTtgtg                                                                                                                                                                   <  1:234187/251‑1 (MQ=255)                                                                             gtgtTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTgcggc                                                                                                                                                               <  1:101701/251‑1 (MQ=255)                                                                                 tGAGCATTTGTTGCGCTACATCTAAGGGCAGGGGGATGGATTCCCATCCTGCCAGTAAACAGGCCAGCGCCTGACGCGGCGGTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCTTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTGAACATACGGAGCAACGTATTTTGCCCCGGCCAGGGCTGCTAATGGCCCTTGTGCGGCGCt                                                                                                                                                            <  2:196263/250‑1 (MQ=255)                                                                                       tttGTCGCGCTACAGCTAAGGTGAGGGGGAGGGATTCCCATCCCGACAGTAACCAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATTCTTTCTGGCGCTTGCATTTCTAACAGCGTTTGCAGCTCCGGAACCGTACGAATGCCGTTTCCGCCCGGGGCATCTACGCGGTTAACATACGTAGCAACTTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCtatata                                                                                                                                                       <  2:164543/249‑1 (MQ=255)                                                                                              cgcTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCTCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCCTTTCCTCCCTGGGCAGCTACGCGGTTAACATACGGAGCAACGTATTTTGCCTCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTg                                                                                                                                               <  1:55139/250‑1 (MQ=255)                                                                                                 tACATCCAGGGGCAGGGTGATGGATTCACATCATGCCGGTACACAGTCCAGCCCCTGACGCGGCGCTTTACAGCTGGCAGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATCACCCTTGTGCGGCGCTATATACAGCAGTGCCa                                                                                                                                            >  2:141817/1‑250 (MQ=255)                                                                                                   cATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgt                                                                                                                                         <  1:38303/251‑1 (MQ=255)                                                                                                        aaGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCTCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCGAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgtgt                                                                                                                                       <  1:1201/248‑1 (MQ=255)                                                                                                                ggggATGGATTCACATCCTGCCAGTAAACAGGCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTTAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTTCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGGGTAGTAATACCct                                                                                                                            <  2:286976/248‑1 (MQ=255)                                                                                                                           cacaCCCTGCCATTAAACAGTCCATCGGCTGACGCGGCGTTGTAAAGCTAGCTGCCAGCACCATTCTTTCTGGCGCGTGCATTTCTAACAGCGTTTTCAGCTCCTGAACCGTACGAATGACGTTTCCGCCCTGGGCATCGACGCGGTTAACATGCGGAGCAATATATTTTGCCCCTGCCAGAGCTGCTAATAACCCTTGTGCGGCGCTATATACATCAGTGCCAAGTGTAGTAATACCCGCTTTTTTCAGt                                                                                                                 <  1:203448/251‑1 (MQ=255)                                                                                                                            acaTCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGAATGCCAGCACCATGACTTCTGGCGCGCGCATATCTAACAGCGCTTGCAGCTCCTGAACCGAACAAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTCCCCCTGCCAGTGCGCCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGt                                                                                                                 >  1:285213/1‑250 (MQ=255)                                                                                                                                    ccAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAAtt                                                                                                        >  1:69707/1‑251 (MQ=255)                                                                                                                                    ccAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAAtt                                                                                                        >  1:297338/1‑251 (MQ=255)                                                                                                                                             aGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCAAGCACCATGATTTCTGGCTCGTGCATTTCTAACAGCGGTTGCAGCTCCTGATCCGTACGAATGCCGTGTCCGCCCTGTGCATCTACGCGGTAAACATACGGAGCAACGTATTTTGCCTGTGCCAGTGCGGCTAATGACCCTTGTGTGGCGCTATATACAGCAGTGCCAATTGTAGTAATACGCGCTTTTTTCAGTATTTTAATTGCTGCCAGa                                                                                               <  1:117237/251‑1 (MQ=255)                                                                                                                                                ccAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCttttt                                                                                                                      <  2:297767/225‑1 (MQ=255)                                                                                                                                                ccAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCttttt                                                                                                                      >  1:297767/1‑225 (MQ=255)                                                                                                                                                          aCGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCCGCTCCTGACCCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCATTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTc                                                                                   >  2:295489/1‑250 (MQ=255)                                                                                                                                                                          ctggccgCCAGCACCATGCTTTCTGGCGCGTGCATTTCTACCAGCGTTTGAAGCTCCTGAAACGTACGAATGCAGTTTCCGCCCTGGGCATCTAAGCGGTTAACATACGGAGAAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTcaccac                                                                  >  1:145092/1‑251 (MQ=255)                                                                                                                                                                                                               cTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTCTTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACACTACCCGGAATAGCGTCGCGCAGGcgcttcgctt                               >  2:197637/1‑249 (MQ=255)                                                                                                                                                                                                               cTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGcgcttcgctt                               <  1:197637/249‑1 (MQ=255)                                                                                                                                                                                                                 aaCAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTAGACATCTACGCGGTTAACATACGGAGCAACGTATTTTACCCCTGCCATTGCGGCTAATAACCCTTGTGCGGCGCTCTATACCGCAGTGCCAAGTGTAGTAATCCCCTCTTTTTTCAGCATTTGAATTGCTGCCAGACCTTCGGAAGTGACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCttcttc                           >  1:278631/1‑251 (MQ=255)                                                                                                                                                                                                                          tGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATccc                  >  1:75370/1‑251 (MQ=255)                                                                                                                                                                                                                            cAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCAGCTACGCGATTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATAGACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTGTCAGTATGTTAATTGCTGCCAGACCTTCGGAGGTCACCGGGATTTTCCCCACAATACCCGGTATAGCGTCGCGCAGGCGCTTCTGTTCGTCCGCCATccctg                <  2:135061/251‑3 (MQ=255)                                                                                                                                                                                                                                 ccTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGGGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑Tgcgc            <  2:297338/249‑1 (MQ=255)                                                                                                                                                                                                                                         gTACGAATGCCGTTTCAGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTc  >  2:244810/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               CCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTC  >  NC_000913/4139043‑4139526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑