Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,423	G→A	V219V (GTC→GTT)	yedJ ←	putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,423	0	G	A	100.0%	71.1 / NA	26	V219V (GTC→GTT)	yedJ	putative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/13);  total (13/13)

CCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTT‑CCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCGACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGC  >  NC_000913/2032183‑2032656                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           ccAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTT‑CCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CAt                                                                                                                                                                                                                                 <  2:223104/251‑1 (MQ=255)            cACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGAATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTT‑CCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGcc                                                                                                                                                                                                                      >  1:143227/1‑251 (MQ=255)            cACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATAATAAATTTGCAGCAGTTTCTCCAGCATTGCATGTGCGGCATTCCCGGAGCTGTATGAATCGGTTACTGATATATTTT‑CCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCCGCGGATGACAACGCATCTATACCC‑TTGTGATCAACACCTT‑CATTCTCCCCCGcc                                                                                                                                                                                                                      >  2:54275/1‑251 (MQ=255)                                  caccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTT‑CCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCAACCCCTT‑CATTCTCCCCCGCCAGTTCGGCAATGAGCTTCGccc                                                                                                                                                                                                >  2:72914/1‑250 (MQ=255)                                                                          aGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTT‑CCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAgctg                                                                                                                                                        <  1:100271/251‑1 (MQ=255)                                                                                gCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTT‑CCTGCATAGATTTCACCGGCAATCTGAGATGGCCGACAGGTTAACATAATTACAGCCCTTTAGGGCCACGCGCTCAGACAGCGGATGAAAACGCATCTATAACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGcc                                                                                                                                                  >  1:279192/1‑251 (MQ=255)                                                                                         ccATTCCCGGTGCTGTATGAAGCGGTTACTGATAAATTCT‑CCGGCATAGATTTCCCCGGCCATCGGAGATGGCCGACAGGTTAACATAATTACCGCCATTTAGGGCCCCGGCCTCAGACAGCGGATGAAAACGCATCTATGCCC‑GTGTGACCAAC‑CCTTGCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGt                                                                                                                                         <  2:231187/250‑1 (MQ=255)                                                                                           aTTCCCGGTGCTGTATGAATCGGTTACTGATATATTTT‑CCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACCGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCAAGCGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGttt                                                                                                                                       >  1:295316/1‑251 (MQ=255)                                                                                                    gCTGTATTAATCGGTGACTGA‑AAATTTTACCTCCAAAGTTTCCACCGTCCATCGGAGATGGCGGACAGGGTAACATAATTACGGCCAGTTAGGGCCACGCGCTCAGCCGGCGGATGAAACCGCATCTATCCCC‑TTGTGATCAACACCGT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGttt                                                                                                                              <  2:299811/251‑1 (MQ=255)                                                                                                       gTATGAATCGGTTACTGATATCTTTT‑CCTGCATAGATTTCACCGGCCATATGAGATGGCCGACAGGATAACATAATTACAGCCATTTAGGGACACGCGCTCAGCCAGCGGGTGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCACCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAACGTGCGATGTGCTGCGCCAGCTCCTTGCCTCTTGCGGTTTGCATGGTTTGCg                                                                                                                           >  1:265592/1‑251 (MQ=255)                                                                                                         aTGAATCGTTTACTGATATTTTTT‑CCTGAATATATTGCACCGACCATCTGAGATGGCCGCCAGGTTAACAAAATTACGGCCATTAGGGGCCACGCGCACACCCAGCGGATGAAAACGCTTCGATCCCC‑GTGTTACCAACACCTT‑CATCCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCGTGCGGTTTGCATGGTTTGCgg                                                                                                                          <  2:254893/250‑1 (MQ=255)                                                                                                             atgggTTCCTGATATATTTT‑CCTGCAAAGATTACACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TGGTGATCAACACCTT‑CATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTGTGCATGGTTTGCGGTAGCt                                                                                                                     <  2:75079/248‑1 (MQ=255)                                                                                                                                t‑CCTGCATAGATTTCCCCGGCCATCTGAGATGGCCGACTGGTTAAAATAATTACAGCCATTTAGGGCCACGCGCCCAGCCAGCGGATGAAAACGCATCTACCACC‑TTGTGATCAACCCCTT‑CATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCGCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGa                                                                                                   <  2:100687/250‑1 (MQ=255)                                                                                                                                   cTGCATAGATTTCCCCGGCCATCTGAGATGGCCGACAGGTTAACATCATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAAt                                                                                                >  2:47027/1‑251 (MQ=255)                                                                                                                                            tttCACGGGCCATCTGAGATGGCCGAAAGGTTAACATAATTACAGCAATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAgcg                                                                                       >  2:143762/1‑251 (MQ=255)                                                                                                                                                   ggCCATCTGAGATGGCCGACCGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCACCACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGCTCCAgcgactagcg                                                                                >  2:264666/1‑244 (MQ=255)                                                                                                                                                       attggagaTGGCCGACAGGTTAACATAATTACAGCCATTTAGGCCCACGCGCTCAGCCAGCGGATGAAACCGCATCTAGCCCC‑GTGTGTCCAACACCTA‑CATGCTCCCCCGCCAGTTCGGCACTGAGCGTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCGGCGCCAGCTGCTTGCCTCGTGCGGTTTGCATGGTTGGCGGTAGGTGCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTa                                                                            <  1:41229/247‑1 (MQ=255)                                                                                                                                                                gcccaccAGGGTAACATAAATACAGCCGTTTAGGGCCACCCTCTCATCCACCGGATGAAACCGCATCAATCCCC‑TTGTGACCAACACCTT‑CATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGc                                                                   <  2:80069/245‑1 (MQ=255)                                                                                                                                                                 gCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGACAGCGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGCGCTTCGCCATACACTCGACTAAAAACTCCGCGTTGTGATGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCg                                                                  >  1:271601/1‑251 (MQ=255)                                                                                                                                                                   caacagataacaagacttACAGCCATTTAGGCCCACGCGTTCAGCCAGCGTATGAAAAGGCATCTATCCCC‑TGGGGATCAACCCCTT‑CATTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACAAAAAAATGCGCGGTGTGCTGCTCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTGCAGCAACGGAGGCTGGAAATGATCCAGCGCTTAGCGTTTAACATCAAGCggg                                                                <  2:303582/235‑1 (MQ=255)                                                                                                                                                                             aCATAATTACAGCCATTTAGGCCCACGCGCTCAGCCAGAGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCtgtg                                                      <  1:13318/251‑1 (MQ=255)                                                                                                                                                                               aTAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCg                                                    >  1:34350/1‑251 (MQ=255)                                                                                                                                                                                   ttACAGCCATTAAGGCCCACGCCCTCAGCCAGCGGAGAAAAACTCATCTA‑CACCCTGGTGATCAACACTTT‑CTTTCCCCCCCGCCAGTTCGGCACGGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGGTCCAGCGCGTAGCGTTTATCATCAAGCGGGCTATGCTGTGCGAAc                                                 <  2:211199/250‑1 (MQ=255)                                                                                                                                                                                       aGCCATTTACGGCCACGCGATCAGCCAGCGGATGAACACGCCTATCTCACC‑TTGTGCTCAACACCTT‑CATTCTCCCCCGCACGTTAGGCACCAGGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGCGCTGCCCCAGCTGCTTGCCTCATGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGGCTGGAAATGATCCAGCGCGTAGCGTTTATCCTCAAGCGGGCGATGCTGTGCGCACGGagc                                            >  2:297865/1‑249 (MQ=255)                                                                                                                                                                                                                cagcGGATGAAAACGCATCTATCACC‑TTGTGATCAACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCCCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGccc                   >  2:144165/1‑251 (MQ=255)                                                                                                                                                                                                                                    atcgcc‑TTGTGATCAACGCCTT‑GAGTCCCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTAGCATCAAGCGGTCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGc  <  1:47027/244‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           CCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTT‑CCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACC‑TTGTGATCGACACCTT‑CATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGGC  >  NC_000913/2032183‑2032656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑