| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 670,635 | A→T | L324Q (CTG→CAG) | holA ← | DNA polymerase III subunit delta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 670,635 | 0 | A | T | 95.7% | 62.0 / ‑6.0 | 23 | L324Q (CTG→CAG) | holA | DNA polymerase III subunit delta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (11/11); minor base G (0/1); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
CATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACAAAATAACC > NC_000913/670396‑670877 | cATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGTTGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAACTCCGTCCGCCCGGGGTTTATGGCACAAAAGAAGAGATAACCCTTCCTGCTCCGCCcac > 2:190143/1‑251 (MQ=255) gCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTg > 1:29941/1‑249 (MQ=255) gACGATGCGGAGTAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGGTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGTTTTATGGCACAACAGAAGAGATAACCCTTCCTGTTCTGCTCACACGGACTGACCGTAATCTTGTTTTTGGGTGAGTTCCGt < 2:121106/251‑1 (MQ=255) cGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAAGTCGCCAGCGTGTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTGCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGATAGAACCCTTGCTGCTCTGCTCACACTTACGGACCGTAAGCTGGGTTGAGGGTGACTTCCGTTCGTGTc < 2:282603/251‑1 (MQ=255) ggaggcaacatattaGAGATGATTTTGAACCCCCTCAGACACATCAAATTCGACAGCCTTTCCACGGGCTTTCGACGCCCATAGTGCACCGGATCAACGCTGCCGCCCAACAGAGCCTGTACAGATTTCATCTCAACAGTCGATAAATACGTCCGCCAGGGGTTCATGGCACAACAGAAGAGATCACCCTTCCTGCTCTGCCCTAACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGGGTcc > 2:181153/11‑250 (MQ=255) cGCCAGCGCTTCCACGGGATTCAGATGACCATAGTGAACCGGATCAAAGGTGCCGCAAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATACATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTACTCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGACTGACGTAACTGCGTCTGACTTAAGCGATTTAACGcc > 2:303697/1‑251 (MQ=255) ccAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCTGAGCAAAGGTGCCTCCAAACAGAGCCTGTAAAGTTTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCGGCTCGGGCCACTCTGAGTTACCGTTATCTTGTGTGAGGTTGAGTGCGGTTCGTGTCTGGATTTGTACTGCCGGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTc < 1:285772/251‑1 (MQ=255) cGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCGCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGcc < 2:212245/251‑1 (MQ=255) gggTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGccc < 1:238839/251‑1 (MQ=255) tttAGATGGCCAGAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAATATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCGCTGCCCACGCTGACTGACCGTAATCTGGTTTGAGGGTGAGTTCCGTTCGTGGCAGGAGTTGCACGGCCTGACGTAACTGCGTCGGACGTAAGCGATTTAACGCCTCGCCCATCATGCCCcgg < 2:269485/250‑1 (MQ=255) cATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCa < 1:106471/251‑1 (MQ=255) cgtaTCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACGTACTGACCGTAATCTTGTTTGAGGGTGATTTCCTTTCGTGTCAGGTGTTGCACGTCGGGACGTAACTGTTTCTGACTTAAGCGTTTTTACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGc < 1:196259/248‑1 (MQ=255) aGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCaa > 2:143298/1‑250 (MQ=255) gaCAGAGCCTGTAAAGATTTCATATCAATAGTCGATAAATACGTCCGCAGGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATATTGTTTGAGGGAGAGTTCCGTTCGTGTCAGGAGCTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATTCCCCGGCGCTTCTGTCATCCCCGATACTTAtacacacacgaccgcc > 2:178341/2‑236 (MQ=255) ccgagCCTGTAAAGAGTTCATAGCAACCGTCGATAAATACGTCCGCCAGGGGCTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGGCTGACCGTAATCTTGTTTGAGGGTGAGTGCCGTTCGTGGCAGGAGTTGCACGGCCTGACGTAAGCGCGTCTGACTTAAGCGACTTAACGCGTCGCCCAACATGCCCCGGCGGTTCTGCCGTACCCGATGCTTATCAAACAGCGCACGCAGt < 2:106624/249‑1 (MQ=255) aaaGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCCTACCCGATGCTTATCAAACAACCCACGCAGTGGCGTATggg > 2:168667/1‑251 (MQ=255) cAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGACGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTTTCAAACAACGCACGCACTGGCGTATGGGCAGACTGGCGttt > 2:148221/1‑251 (MQ=255) cGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTa < 1:143298/251‑1 (MQ=255) gATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTaa < 1:281783/251‑1 (MQ=255) gggttATGGCACAACAGAAGAGATAACCCTTCCTGCTCTGCACACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCTGTTCGTGTCCGGCGTTGCACGGCCTGACGTAACTGAGTCTGACTTAAGCGAATTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGGCATACCCGATGCTTATAAAACAACGAACGAAGGGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGAAAAAACAggtaacg > 1:144736/4‑245 (MQ=255) acaacaGAAGAGATAACCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAAtgtg < 2:62075/251‑1 (MQ=255) aaCCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGACCCGGCGGGTCTGCCATACCCGCTGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAAAAACAGTTCCCGCTGTAATGTGCGTAAACAAATAAc > 2:66467/1‑251 (MQ=255) aCCCTTCCTGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACAAAATAAcc > 1:20939/1‑251 (MQ=255) | CATGTGTTTACGCTGCACGCTGTTCGCTTCCGGCTGGGGACGATGCGGAGGAACATTATTAGGGATGATTGTGACCCGCGTCAGACCAATCAAATTCGCCAGCGTTTCCACGGGTTTTAGATGACCATAGTGCACCGGATCAAAGGTGCCGCCAAACAGAGCCTGTAAAGATTTCATATCAACCGTCGATAAATACGTCCGCCAGGGGTTTATGGCACAACAGAAGAGATAACCCTTCCAGCTCTGCCCACACTGACTGACCGTAATCTTGTTTGAGGGTGAGTTCCGTTCGTGTCAGGAGTTGCACGGCCTGACGTAACTGCGTCTGACTTAAGCGATTTAACGCCTCGCCCATCATGCCCCGGCGGTTCTGCCATACCCGATGCTTATCAAACAACGCACGCAGTGGCGTATGGGCAGACTGGCGTTTCAGGTTAACCAGTAACAACAGTTCACGTTGTAATGTGCGCAACAAAATAACC > NC_000913/670396‑670877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |