Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,985,726	A→G	intergenic (‑134/‑121)	yqeF ← / → yqeG	putative acyltransferase/putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,985,726	0	A	G	100.0%	87.8 / NA	26	intergenic (‑134/‑121)	yqeF/yqeG	putative acyltransferase/putative transporter YqeG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/12);  total (14/12)

CACGACCAGACTACCAAGTTCCACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGT  >  NC_000913/2985491‑2985962                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             cACGACCAGACTACCAAGTTCCACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACt                                                                                                                                                                                                                                <  1:97965/250‑1 (MQ=255)                         ggcgtAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCGTTCATCACAGCCACGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATGACAGTTATCGTAATTGTTTAAAAAAAGTGATGTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACAGAACTGGCACGTAGTGTTTGTACCCACTAACCCACCAATAGAGGGGTAGATAg                                                                                                                                                                                                       <  2:116153/251‑1 (MQ=255)                                                 acGAAAGCAGCCGATAGGTGTCCGTAACGCCACGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAAATGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAcc                                                                                                                                                                               <  1:111574/251‑1 (MQ=255)                                                               tAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTaa                                                                                                                                                                  >  1:71485/1‑250 (MQ=255)                                                                aagtgtCCGTAACGCCCCGACAATCACAACGTCTTTCATCAAAACCTCGCACCAAGTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGTGACATAACTGGCACGTAGGGTTTGCAACCCCTAACCCTCCAATAGAGGGTTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTaaa                                                                                                                                                                 <  2:14984/248‑1 (MQ=255)                                                                     tCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTACCAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTaa                                                                                                                                                                                                                              >  2:67297/1‑184 (MQ=255)                                                                     tCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTaa                                                                                                                                                                                                                              <  1:67297/184‑1 (MQ=255)                                                                                 cGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGttt                                                                                                                                               <  1:229463/251‑1 (MQ=255)                                                                                      aTCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGTGTAGATAGGGCGTTAATCTCCCATTCTTAACCGGGTTTATGGTAAATTGCCCTCCATTCTGTTTAAttt                                                                                                                                          <  1:287106/251‑1 (MQ=255)                                                                                      aTCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTGTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTGGCAACCACTAACCCACCAATAGAGGTGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAAttt                                                                                                                                          <  1:266583/251‑1 (MQ=255)                                                                                           aaCGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGtt                                                                                                                                                                          <  2:172869/214‑1 (MQ=255)                                                                                           aaCGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGAGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGtt                                                                                                                                                                          >  1:172869/1‑214 (MQ=255)                                                                                                                     aaTTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGAAATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaacaaccccgac                                                                                                            >  1:192098‑M1/1‑235 (MQ=255)                                                                                                                               gCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAACAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccgggg                                                                                                 >  1:300920‑M1/1‑225 (MQ=255)                                                                                                                               gCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccgggg                                                                                                 >  2:76440‑M1/1‑225 (MQ=255)                                                                                                                                                         gTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattt                                                                       <  1:240617‑M1/251‑53 (MQ=255)                                                                                                                                                                           aaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAAATGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgg                                                     >  2:300147‑M1/1‑181 (MQ=255)                                                                                                                                                                            aaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccggg                                                    >  1:235992‑M1/1‑180 (MQ=255)                                                                                                                                                                             aaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGCGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTACATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttcccggacgcatcgccggg                                                    >  2:151771‑M1/1‑179 (MQ=255)                                                                                                                                                                                   aTTTTTATCACAAAGGGAATATGCCTGAGCAGCCGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACGTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgtcaacagaa                                             <  2:57040‑M1/251‑79 (MQ=255)                                                                                                                                                                                     ttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccgggcgcatcgccgggaacagaat                                            <  1:96613‑M1/250‑80 (MQ=255)                                                                                                                                                                                      tttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaata                                           >  2:57436‑M1/1‑170 (MQ=255)                                                                                                                                                                                           cacaAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcg                                     >  1:102499‑M1/1‑165 (MQ=255)                                                                                                                                                                                                   aaaTATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggt                             >  1:10316‑M1/1‑157 (MQ=255)                                                                                                                                                                                                                              aTAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGATTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggacgcatcgccgggaacagaataacgtcgcggatggtatggctgttggtgaacagcattaccat  >  1:168270‑M1/1‑130 (MQ=255)                                                                                                                                                                                                                              aTAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGCAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTCTGTTTAATTTGTAGATGATACGTTCtaaaaaaaccccgacatttgccggggttgtgagcataacgtaatgcttattttaccggaagcatcgccgggaacagaataacgtcgaggatggtatggctgttggttgacagcaataacat  >  2:90252‑M1/1‑130 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             CACGACCAGACTACCAAGTTCCACGGCGGAATGACCCGCTAACGCACCACGAAAGCAGCCGATAGGTGTCCGTAACGCCCCGACAATCACAACGTCTTTCATCACAACCTCGCACCAATTAACAGTGCAATAGTAAATGATTGTTAATTACAGTTATCTTAATTGTTTAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGTTTATGGTAAATTGCCCTCCATTTTGTTTAATTTGTAGATGATACGTTCAGATAATGTCTAATATTTGGTCTAAAGAAGAAACTCTGTGGAGTTTCGCGCTCTACGGCACAGCCGTTGGTGCAGGCACGCTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGT  >  NC_000913/2985491‑2985962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑