Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,628,332	T→C	intergenic (+69/‑20)	ydfG → / → rspR	3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,628,332	0	T	C	100.0%	84.3 / NA	23	intergenic (+69/‑20)	ydfG/rspR	3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

ACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTC  >  NC_000913/1628097‑1628547                                                                                                                                                                                                                                            |                                                                                                                                                                                                                        aCGGTAAAGCAGAAAGAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGCGGTAGACTCATGCAGTTAACCCACTCACtacatga                                                                                                                                                                                                           >  2:53235‑M1/1‑243 (MQ=255)                   ccgatcaAAATACCGCTGCATTGACGCAAGAAGATGTCCGCGAAGCCGTCTGGTGGGAGTCAACGCTGCCTGCTCACGTCAATCTACATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGACTGTCCACCGTCAGTCATTTTTCTACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttcg                                                                                                                                                                                        >  2:211203‑M1/4‑225 (MQ=255)                             aacCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctc                                                                                                                                                                              <  2:4021‑M1/250‑37 (MQ=255)                                      aTTGACGCCAGAAGATGTCAGCGAAGCCGTCGTGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttatgtccgttctcaataccaaa                                                                                                                                                                     <  2:16337‑M1/251‑46 (MQ=255)                                         gACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaatt                                                                                                                                                                   <  1:238641‑M1/250‑48 (MQ=255)                                                         aGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTCCTCACGTCAATATCAATCCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCTTCAGTAATTTTTAGACCCGGCTTAACTGCCGTGTTATTTCTTGGCGCAAAAAAGTGGTAGACTCAGTCAGTGAACCCACTCACtacatgaacagccgcaggacctttagctccgttctcaacaccaaattcaacttcctgattctca                                                                                                                                                  <  1:147087‑M1/251‑65 (MQ=255)                                                                                  cGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATGCCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctct                                                                                                                         <  1:62513‑M1/251‑90 (MQ=255)                                                                                       cctgcTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaatt                                                                                                                    <  2:251645‑M1/251‑95 (MQ=255)                                                                                                          ataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGACTGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggaactttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaac                                                                                                  >  2:285714‑M1/1‑138 (MQ=255)                                                                                                              ccTGGAAATGATGCCGGTTACCCAACGCTATGCCGGACTGCATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaatacccaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatc                                                                                             >  2:177149‑M1/1‑134 (MQ=255)                                                                                                                             ggTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatcttt                                                                              <  2:82170‑M1/251‑133 (MQ=255)                                                                                                                             ggTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatcttt                                                                              <  1:9554‑M1/251‑133 (MQ=255)                                                                                                                                                ggACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaa                                                           <  1:126072‑M1/251‑152 (MQ=255)                                                                                                                                                 gACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGCGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaac                                                          <  1:53235‑M1/251‑153 (MQ=255)                                                                                                                                                                        tttttATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaacc                                        >  1:263379‑M1/1‑76 (MQ=255)                                                                                                                                                                        tttttATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaacc                                        <  2:263379‑M1/246‑171 (MQ=255)                                                                                                                                                                                 ccGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaa                                                                                                            <  2:86188‑M1/169‑103 (MQ=255)                                                                                                                                                                                 ccGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaa                                                                                                            >  1:86188‑M1/1‑67 (MQ=255)                                                                                                                                                                                                  ttATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCCCTCACtacatgaacagcggcagcacccttaggtccgatcacaataccaaattcaacttcctgattctcagttaatgttttgacctcgttgctctgaattgctgagaactagacaaacacatctttgctgccatctttccgcgtgatgaaaccaaaaactttttcagggttaaaccatttcactaaaccagtcattttgttagacat         >  1:157054‑M1/1‑50 (MQ=255)                                                                                                                                                                                                        cTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattat   >  1:75484‑M1/1‑44 (MQ=255)                                                                                                                                                                                                          tGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctgtaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattatt  <  2:2931‑M1/250‑209 (MQ=255)                                                                                                                                                                                                             cacaAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgtttt                                                                                                                                       <  2:213860‑M1/114‑76 (MQ=255)                                                                                                                                                                                                             cacaAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgtttt                                                                                                                                       >  1:213860‑M1/1‑39 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                        ACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTC  >  NC_000913/1628097‑1628547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑