Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,312,151	G→C	Q281E (CAA→GAA)	alsR ←	DNA‑binding transcriptional repressor AlsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,312,151	0	G	C	100.0%	107.3 / NA	29	Q281E (CAA→GAA)	alsR	DNA‑binding transcriptional repressor AlsR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/14);  total (15/14)

GCCTTTTTTCATATCTACCCAAAATGGGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAC  >  NC_000913/4311908‑4312370                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                            cgctccagttaatcaaatcacgcattcgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCATGGTGAGAAGAAAGCAACAATTGCGCCGGTTTTTTCCatattaa                                                                                                                                                                                                                     <  2:227219‑M2/102‑1 (MQ=255)            atcaaatcacgcatacgcgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGt                                                                                                                                                                                                          >  2:171979‑M2/139‑251 (MQ=255)                     cgcatacgcgctctcgactaccgtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactctttattcaaacctctgacgaacaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTAAGCGCGCCTGGTGAGAAGAAATCAACACTTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTg                                                                                                                                                                                                 >  1:126456‑M2/130‑251 (MQ=255)                        atacgcgctctcgactacagtatgcatcttttatgccacattttatgtgggggcattccctgatattccgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGCAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGCGAGAAGAAATCCACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGc                                                                                                                                                                                              >  1:186990‑M2/127‑251 (MQ=255)                        atacgcgctctcgactacagtatgcatcttttatgcaacattttatgtggggtcattccctgatattacgggcactatttattccaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGc                                                                                                                                                                                              >  1:261513‑M2/127‑251 (MQ=255)                             cgctctcgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTGGTTCAATGTTGAGCTGGGc                                                                                                                                                                                         <  1:79961‑M2/130‑1 (MQ=255)                                  ccgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCGTTTTCTTATTTCAGCGCGCCTTGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATAGTAGCTTGTTCAATGTTGAGCTGGGCGACag                                                                                                                                                                                    <  2:270651‑M2/135‑1 (MQ=255)                                   cgactacagtatgcatcttttatgccacattttatgtggggtcattccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGAc                                                                                                                                                                                      >  2:232933‑M2/116‑248 (MQ=255)                                        acagtatgcatcttttatgccacattttatgtggggtcattccctgatattgcgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTTATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGAc                                                                                                                                                                                <  1:268695‑M2/139‑1 (MQ=255)                                                                              attccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTCGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGAAAGGTTGGTTGTAATATTCTTGcc                                                                                                                                        >  1:201050‑M2/73‑251 (MQ=255)                                                                                tccctgatattacgggcactatttattcaaaactctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCg                                                                                                                                       >  2:70570‑M2/71‑250 (MQ=255)                                                                                                                  ctgacgaaaaacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCt                                                                                                    >  1:189308‑M2/37‑251 (MQ=255)                                                                                                                           aacaggctgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCa                                                                                            >  1:107264‑M2/28‑250 (MQ=255)                                                                                                                                  tgtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGCTGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAataatat                                                                                    >  1:19758‑M2/21‑251 (MQ=255)                                                                                                                                  ggtcgtcagttttgacttgaGGTAAGGGGACA‑GTAACTCCGCGATGGCTGAATTTCCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCTGTTTTTTCCATATTAATATTAGCTTGTTCAAGGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAAGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAagaatat                                                                                    <  2:205064‑M2/231‑1 (MQ=255)                                                                                                                                   gtcgtcagttttgacgtgaGGTAAGGGGACA‑GTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAaataatatg                                                                                   <  1:185260‑M2/232‑2 (MQ=255)                                                                                                                                                 cgtgaGGTTAGGGGACA‑GTAACTCCGCGATGGCGGAATGACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGGTTTTTCCATATTAATATTAGCTTGTTCGATGTTGAGCTGGGCGACTGAGACAAAAAAAGCGTCCAGCAATGTTGGTTGTGATATTCTTGCCGAGGCAGTACGACCTAATAACGGCGTTTCGGGGGCTGGTGAGCAAATAATATTATCGGCCAGTTTCg                                                                     <  1:291477‑M2/246‑1 (MQ=255)                                                                                                                                                        tAAGGGGACAG‑TAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCgg                                                                              <  1:6762/235‑1 (MQ=255)                                                                                                                                                        tAAGGGGACAG‑TAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGAGCGCCTGGCGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTATTATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCgg                                                                              >  2:6762/1‑235 (MQ=255)                                                                                                                                                         aaGGGGACAG‑TAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCggg                                                             >  1:173024/1‑250 (MQ=255)                                                                                                                                                                         ccGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTAt                                                <  1:171979/249‑1 (MQ=255)                                                                                                                                                                            cgATGGCGGAATTACGTTTTTCTTCTTTCAGTGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACACAACAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTCGCTCTGGGtt                                           >  2:109916/1‑251 (MQ=255)                                                                                                                                                                                   ggAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACaaa                                    <  1:232933/251‑1 (MQ=255)                                                                                                                                                                                     aaTTACCTTTTTCTTATTTCAGATCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGCGCAAATAATATGATCGGCCAGTTTCGCTACCGGGGAATGGTAGCTATGGGTTATTCAaat                                   >  2:256177/1‑250 (MQ=255)                                                                                                                                                                                         aCCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGACGGTTTTTTCCATATTAATATTAGCTTGGTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGTGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATc                               <  1:175539/250‑1 (MQ=255)                                                                                                                                                                                                       tCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCtt                 <  1:65555/250‑1 (MQ=255)                                                                                                                                                                                                                     gagaAGAAATCAACAATTGCGCCGGTTTTGTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAc  <  2:201050/251‑1 (MQ=255)                                                                                                                                                                                                                       gaagaaATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAaat                                   <  2:100152/216‑1 (MQ=255)                                                                                                                                                                                                                       gaagaaATCAACAATTGCGCCGGTTTTTTCCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAGGTTGGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATGATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAaat                                   >  1:100152/1‑216 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                            GCCTTTTTTCATATCTACCCAAAATGGGTTGGAGAGGGTTTTCAATACGACAGCATATTCGGCGGCAGCAAAAGCGCTGGTTGACAACATTAAGCCCACGAGTGTGCCGCTGAAATATTTCAGATATTTATTCATAATAATGTTCTCACGGTAAGGGGACAGGTAACTCCGCGATGGCGGAATTACCTTTTTCTTATTTCAGCGCGCCTGGTGAGAAGAAATCAACAATTGCGCCGGTTTTTTGCATATTAATATTAGCTTGTTCAATGTTGAGCTGGGCGACAGAGACAAAAAAAGCGTCCAGCAAAGTTAGTTGTAATATTCTTGCCGAGGCATTACGACCTAATAACGGCGTTTCCGGGGCTGGTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTAC  >  NC_000913/4311908‑4312370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑