| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,139,511 | Δ1 bp | coding (198/663 nt) | fsaB ← | fructose‑6‑phosphate aldolase 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,139,511 | 0 | C | . | 94.7% | 73.1 / ‑3.5 | 19 | coding (198/663 nt) | fsaB | fructose‑6‑phosphate aldolase 2 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (10/8); minor base T (0/1); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01 | |||||||||||
CCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGT > NC_000913/4139267‑4139726 | ccTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGGGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑Tgcgc < 2:297338/249‑1 (MQ=255) gTACGAATGCCGTTTCAGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTc > 2:244810/1‑251 (MQ=255) gCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCGAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATGTTAATTGCTGCCAGACCTTCGGAAGTCGCCGGGATTTTCACCACAATATCCGGAATAGCGCCGCGCAGGCGCGTCGCTTCTTCCTCCA‑GCCTTGCGCTTCGCGGCTCATGGTCGGATCATACAGAATGccc < 2:48029/251‑1 (MQ=255) gTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCTGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTGAATTGCTGCCAGTCCTTCGGAAGGCTCCGGGATTTTCACTACAATACCCGGAATATCGTCGTGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCTTCGCGGCTCATGGGCTGAGCAAACAGAATGCCCTCATCACCAAt < 1:40217/251‑1 (MQ=255) gTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAAACCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCAcccat > 2:24525/1‑248 (MQ=255) aTACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCttt > 1:263622/1‑251 (MQ=255) aaCGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGAcg > 1:185616/1‑251 (MQ=255) gCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGa < 1:24525/250‑1 (MQ=255) ataaCCCTTGTGCGGCGCTATATACAGCAGTGCGAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCACTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGCCTCCTTGc > 2:218488/1‑251 (MQ=255) tgtgCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGc < 1:2254/250‑1 (MQ=255) ctTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCTGGTTCGTTGTCACCCCGGCCATGGGGACTATGc > 2:278578/1‑251 (MQ=255) tCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCg > 1:30480/1‑251 (MQ=255) gAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGt < 1:42272/193‑1 (MQ=255) gAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGt > 2:42272/1‑193 (MQ=255) aCCGGGATTTTCACCACATTACCCGGAATAGCGGCGCGCAGGCGCTTCACTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGATCAAACAGAATGCCTTCAGCACCAATCGCTTTTTGCATACGCGGCAGCTCTTCCCATATGGATTCCTTGCTGGCAGCGATAATGCTCGGGTTTTTTTTCACCCCGGCAATTGGGAATATGCTTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGa < 1:185913/251‑1 (MQ=255) caccacAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCat > 1:54276/1‑251 (MQ=255) aaTACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGATCAATCAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCATCGATAATGTTCGGGTTAGTTTTCACCCCGGCAATGGGGAATATGCGTGCCATACGTTCGACTTCTGCGACGTTAGCGTTGTCCAGATACAGTTCCATGATGtt < 2:208770/251‑1 (MQ=255) tAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTc < 2:230307/201‑1 (MQ=255) tAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTc > 1:230307/1‑201 (MQ=255) aGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGGCGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTAcagt < 1:141817/251‑1 (MQ=255) atcccTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCATCACTGCCCATATGTACGCCTTGCGTGCAGCGATAATGCTc < 2:32795/114‑1 (MQ=255) atcccTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCACTCATCACCAATCGCTTTTTGCAGACGAGGCAGAAATTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTc > 1:32795/3‑116 (MQ=255) | CCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGT > NC_000913/4139267‑4139726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |