Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,867,454 2 bp→AA coding (97‑98/993 nt) rpoS ← RNA polymerase sigma factor RpoS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,867,4540TA97.4% 125.9 / NA 38Q33M (CAG→TTG) rpoSRNA polymerase sigma factor RpoS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (15/22);  minor base C (1/0);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01
*NC_0009132,867,4550GA97.4% 129.2 / NA 38Q33M (CAG→TTG) rpoSRNA polymerase sigma factor RpoS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (15/22);  minor base C (1/0);  total (16/22)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

ACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTC  >  NC_000913/2867213‑2867696
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                 
aCCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctacta                                                                                                                                                                                                                                           <  2:139022/251‑1 (MQ=255)
    cGATTGCCATAACGGCGGGCAATTTTGACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACTCGCAAAATAAACTTCTGCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGc                                                                                                                                                                                                                                       <  2:255299/251‑1 (MQ=255)
         gCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtttt                                                                                                                                                                                                                                  <  1:41288/251‑1 (MQ=255)
              aaCGGCGGGCAATTTTTACCACCAGACGCAAGTTACGCTCGATCATCCGGCGGCGAGAGGCGACATCTCGACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCGCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCa                                                                                                                                                                                                                             <  2:169959/251‑1 (MQ=255)
                       cAATTTTTACCACCAGACGCAAGTTACTCTCGATCGTCCGGCGGCGAGAGGCAACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGATGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACTCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCCAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGGCAAAAACCTCa                                                                                                                                                                                                                    <  2:302453/251‑1 (MQ=255)
                           ttttACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTc                                                                                                                                                                                                                >  2:81053/1‑251 (MQ=255)
                               cccaccaGGCGCAAGTTTCTCGCGATCATCCGGCGGCGAGAGGCGACAGCTCCGCGCAGTGCGCGACGCGCAAAATAAACTTCTGCTTCGGCCGTTAACAGTGGTGAAGAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGGTTGGCTCCCTGCGATAACATTGCCTCTTCGGCCAAATCGGTATCACTGGGTTGCAATTCTACTAAGGCCTTTGCGGCTAGAACCTCAGCTCCGtt                                                                                                                                                                                                            <  2:126579/250‑1 (MQ=255)
                                 caccaGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACGCCGTtct                                                                                                                                                                                                          <  2:16869/251‑1 (MQ=255)
                                                cctctCGATCATCCGGCGGCGAGCGGCGACATCTCCACGCAGTGCGCGCCGCGCACCCTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATATCACCAAGGTAAAGCTGAGTCGCGTCCACCACAAGCTGTGTGGCCACCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAACGCCTTTTCCTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCAt                                                                                                                                                                                           >  2:26867/2‑251 (MQ=255)
                                                      gATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCCCTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCAt                                                                                                                                                                                     >  2:146886/1‑251 (MQ=255)
                                                       agcatcCGGCGGCGAGAGGCGCCATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAATCTGAGTCGCGTCCAACACACGCTGTTTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACGGGGTTCCAAGTCTACTAAGGCCTGTTCGTCAAAAGCCTCAACCTCGTTCTCATCAAATTCCGCATCTTCAtt                                                                                                                                                                                    <  2:143554/249‑1 (MQ=255)
                                                             cggcggCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATc                                                                                                                                                                              <  1:81053/251‑1 (MQ=255)
                                                              ggcggcGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCCATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCACATCGTTATCACTGGGCTCCCATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCACATTCCGCATCTTCATTTaactc                                                                                                                                                                              >  2:85573/1‑247 (MQ=255)
                                                              ggcggcGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATc                                                                                                                                                                              >  2:77093/1‑250 (MQ=255)
                                                                  gcgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTGATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAGTCCGCATCTTCATTTAAATCATGaa                                                                                                                                                                         <  1:147450/251‑1 (MQ=255)
                                                                      gagGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACttt                                                                                                                                                                     <  1:79943/251‑1 (MQ=255)
                                                                           gACATCCCAACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTCCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCAGCTTCATTTAAATCATGAACTTTCAGCg                                                                                                                                                                <  2:3241/251‑1 (MQ=255)
                                                                            aCATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTCACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACCCACGCTGTGTGGCTCACTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAACAACCTCAACTCCGTTCTCCTCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGt                                                                                                                                                               >  1:130589/1‑251 (MQ=255)
                                                                                   cACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGTGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGa                                                                                                                                                        <  1:255914/251‑1 (MQ=255)
                                                                                   cACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTCAGTAGCGACCACAACACGATGTGTGGCTCCCTGCGATAACAGTTCATCTTCGGCTAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTAAAAAACCTCAACTCAGTTCTCATCAAATTCCGCAACTTCATTTAAATAATGAACTTTCAGCGTATTcagc                                                                                                                                                        >  1:175474/1‑248 (MQ=255)
                                                                                             gcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGCGAATACCCAATCTCACCAAGGTACAGCTGAGCCACGTCCAACCCACGCTCAGTGGCTCCCTGAGATCACAGTTCATCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAACAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGt                                                                                                                                              >  1:135055/1‑251 (MQ=255)
                                                                                                                      tcGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGCTCCAATTCTACTAAGGCCTTTTCGTCAAAAAACTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACg                                                                                                                     >  2:226060/1‑251 (MQ=255)
                                                                                                                          ccGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAAc                                                                                                                 <  1:156550/251‑1 (MQ=255)
                                                                                                                                 cAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACCCACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCa                                                                                                            >  1:295747/1‑249 (MQ=255)
                                                                                                                                     ggtgAATAACAAATCGCACCAAGGAAAAGCTGAGTCGAGTCCAACACACGCTGAGTGGCTCACTGCGCTACCAGTTCCTCTTCGGCCAAATCGTTATCACTGTGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCTTAAGGAGGCTCCTACCAGTGATCCCTTGACGGAACATTCAAGCaa                                                                                                       >  2:144837/1‑250 (MQ=255)
                                                                                                                                      gtgAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGATGGAACATTCAAGCaaa                                                                                                      >  1:196156/1‑250 (MQ=255)
                                                                                                                                            aaCCAATCTCTCCAAGGGAAAGCTGAGTCGCGTCCAACTCCCGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTTGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTg                                                                                                <  2:101403/250‑1 (MQ=255)
                                                                                                                                                aaTCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCAGAAGGTGGCTCCTACCCGTGATCCCTTGATGGAACATTCAAGCAAAAGCCTGGTTcc                                                                                           <  2:196156/251‑1 (MQ=255)
                                                                                                                                                       ccAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGAtt                                                                                    <  1:8181/251‑1 (MQ=255)
                                                                                                                                                                         gcgTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATaa                                                                  <  1:146886/251‑1 (MQ=255)
                                                                                                                                                                           gTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTGCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAGTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGAGCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACg                                                                <  1:205034/251‑1 (MQ=255)
                                                                                                                                                                              cAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGACAAATAACGCAg                                                             <  1:226060/251‑1 (MQ=255)
                                                                                                                                                                                   cacGCTGTGTGGCTCCCTGCGATAACAGTTACTCTTCGGCCAAATCGTTCTCACTGGGTTCCACTTCTATTAAGGCCATTTCGTCAAAAACCTCACCACCGGTATCATCCAATTCCGCATCTTAATTTAAATCATGAACATTCAGCGTATTCTGACTCCTACGGTGGCTCCTACCCGTGCTCCCTTTACGGAACCTTCAAGCAAAGGCCTGGTTCCGCAGATTTATAGCTGCTGCAAATAACGCAGCGGGt                                                        >  2:270875/1‑251 (MQ=255)
                                                                                                                                                                                        tgtgtgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAACGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACg                                                   >  2:280159/1‑251 (MQ=255)
                                                                                                                                                                                                     gCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTTGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCGCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGt                                      <  2:252061/251‑1 (MQ=255)
                                                                                                                                                                                                                    ctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAAt                       >  2:128770/1‑251 (MQ=255)
                                                                                                                                                                                                                              aTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCgtgt             >  1:100322/1‑251 (MQ=255)
                                                                                                                                                                                                                                          gggTTCCAATTCTACTAATGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCATCGTATTCTTACTCATAAGGTGGCTCCTTCCCTTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGTTTTATCGCTGCGGCAAATAGCGCAGCGGGTTTACGGGTTGCCCCCTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTc  <  1:298447/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                                 
ACCACGATTGCCATAACGGCGGGCAATTTTTACCACCAGACGCAAGTTACTCTCGATCATCCGGCGGCGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTGTTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTAAATCATGAACTTTCAGCGTATTCTGACTCATAAGGTGGCTCCTACCCGTGATCCCTTGACGGAACATTCAAGCAAAAGCCTGGTTCCGCCGATTTATCGCTGCGGCAAATAACGCAGCGGGTTTACGGATTTCCCCTTGTAACGAATTTCAAAATGCAAGCGTGTTGAACTGGTTC  >  NC_000913/2867213‑2867696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑