breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,446 T→C S216P (TCC→CCC)  thrB → homoserine kinase
RA 39,750 C→T D213N (GAC→AAC)  caiA ← crotonobetainyl‑CoA reductase
RA 148,502 G→A I98I (ATC→ATT panC ← pantothenate synthetase
RA 169,785 A→G E3G (GAA→GGA)  fhuC → iron(III) hydroxamate ABC transporter ATP binding subunit
RA 209,247 G→A L209L (CTG→CTA accA → acetyl‑CoA carboxyltransferase subunit alpha
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 301,516 G→A D124D (GAC→GAT paoB ← aldehyde dehydrogenase, FAD‑binding subunit
RA 320,156 T→C intergenic (‑155/‑71) rclA ← / → rclR cupric reductase RclA/DNA‑binding transcriptional activator RclR
RA 332,030 C→T intergenic (+154/‑341) yahV → / → pdeL protein YahV/DNA‑binding transcriptional regulator/c‑di‑GMP phosphodiesterase PdeL
RA 358,005 T→C E229G (GAG→GGG)  cynR ← DNA‑binding transcriptional dual regulator CynR
RA 405,638 T→C K3E (AAG→GAG)  proC ← pyrroline‑5‑carboxylate reductase
RA 474,452 C→T R237H (CGC→CAC)  tesB ← acyl‑CoA thioesterase II
RA 493,485 C→T A465V (GCG→GTG)  dnaX → DNA polymerase III subunit tau
RA 495,201 A→T K28* (AAA→TAA)  htpG → chaperone protein HtpG
RA 495,211 T→C F31S (TTC→TCC)  htpG → chaperone protein HtpG
RA 550,643 G→A E69K (GAA→AAA)  ybcF → putative carbamate kinase
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
RA 581,072 G→A G80E (GGG→GAG)  nohD → putative DNA‑packaging protein NohD
MC JC 676,678 Δ11,173 bp IS5‑mediated ybeRgltI ybeR, djlB, ybeT, ybeU, djlC, hscC, rihA, gltL, gltK, gltJ, sroC, gltI
RA 699,728 C→T G200D (GGC→GAC)  umpH ← UMP phosphatase
RA 699,843 C→T G162R (GGG→AGG)  umpH ← UMP phosphatase
RA 727,250 C→T G495R (GGG→AGG)  kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 736,715 C→T intergenic (+16/‑1377) ybfQ → / → ybfD inactive transposase YbfQ/H repeat‑associated putative transposase YbfD
RA 737,727 C→T intergenic (+1028/‑365) ybfQ → / → ybfD inactive transposase YbfQ/H repeat‑associated putative transposase YbfD
RA 748,987 C→T A241T (GCG→ACG)  ybgP ← putative fimbrial chaperone YbgP
RA 749,898 G→A R758C (CGC→TGC)  ybgQ ← putative fimbrial usher protein YbgQ
RA 753,942 C→A C176F (TGT→TTT)  gltA ← citrate synthase
RA 754,042 C→T A143T (GCG→ACG)  gltA ← citrate synthase
RA 798,989 C→T S135L (TCA→TTA)  pgl → 6‑phosphogluconolactonase
RA 817,983 C→T H314Y (CAT→TAT)  moaA → GTP 3',8'‑cyclase
RA 826,476 C→T G259G (GGG→GGA ybhS ← ABC exporter membrane subunit YbhS
RA 832,323 C→T intergenic (+87/+145) rhlE → / ← ybiA ATP‑dependent RNA helicase RhlE/N‑glycosidase YbiA
RA 839,315 C→T K739K (AAG→AAA fiu ← iron catecholate outer membrane transporter Fiu
RA 842,254 G→T intergenic (‑198/‑78) mcbA ← / → rlmF DUF1471 domain‑containing protein McbA/23S rRNA m(6)A1618 methyltransferase
RA 842,367 C→T N12N (AAC→AAT rlmF → 23S rRNA m(6)A1618 methyltransferase
RA 848,750 C→T M54I (ATG→ATA dps ← DNA protection during starvation protein
RA 848,859 C→T R18H (CGC→CAC)  dps ← DNA protection during starvation protein
RA 857,132 C→T D390D (GAC→GAT ybiT → putative ATP‑binding protein YbiT
RA 941,264 C→T R102R (CGC→CGT dmsA → dimethyl sulfoxide reductase subunit A
RA 943,457 C→T T15I (ACC→ATC)  dmsB → dimethyl sulfoxide reductase subunit B
RA 946,025 C→T T52I (ACC→ATC)  ycaD → putative transporter YcaD
RA 947,423 C→T T65T (ACC→ACT ycaM → putative transporter YcaM
RA 953,214 C→T G114D (GGT→GAT)  pflB ← pyruvate formate‑lyase
RA 954,560 C→T intergenic (‑94/+312) focA ← / ← ycaO formate channel FocA/ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 956,501 C→T W44* (TGG→TGA ycaO ← ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 978,478 C→T S718L (TCA→TTA)  mukB → chromosome partitioning protein MukB
RA 984,313 C→T intergenic (+16/+206) gloC → / ← aspC hydroxyacylglutathione hydrolase GloC/aspartate aminotransferase
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 989,027 C→T intergenic (‑42/+127) asnS ← / ← pncB asparagine‑‑tRNA ligase/nicotinate phosphoribosyltransferase
RA 1,004,748 C→T intergenic (+91/‑20) ycbF → / → pyrD putative fimbrial chaperone YcbF/dihydroorotate dehydrogenase, type 2
RA 1,007,605 C→T intergenic (‑5/‑239) ycbX ← / → rlmL 6‑N‑hydroxylaminopurine resistance protein/fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
RA 1,020,516 G→A A135V (GCA→GTA)  sulA ← cell division inhibitor SulA
RA 1,023,189 C→T R232Q (CGG→CAG)  yccS ← putative transporter YccS
RA 1,043,505 C→T G236S (GGT→AGT)  etk ← protein‑tyrosine kinase Etk
RA 1,072,103 A→G V19V (GTT→GTC rutC ← 3‑aminoacrylate deaminase
RA 1,102,966 C→T R77R (AGG→AGA csgD ← DNA‑binding transcriptional dual regulator CsgD
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,105,778 C→T intergenic (+53/‑42) ymdA → / → ymdB uncharacterized protein YmdA/2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity
RA 1,119,790 C→T G266D (GGC→GAC)  solA ← N‑methyl‑L‑tryptophan oxidase
RA 1,122,356 C→T S84S (TCG→TCA pyrC ← dihydroorotase
RA 1,123,620 C→T K145K (AAG→AAA grxB ← reduced glutaredoxin 2
RA 1,133,180 A→G T203A (ACC→GCC)  flgE → flagellar hook protein FlgE
RA 1,136,605 G→A G111E (GGA→GAA)  flgI → flagellar P‑ring protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase
MC JC 1,286,718 829 bp→C [ychS][tyrT] [ychS], rttR, tpr, tyrV, [tyrT]
RA 1,287,588 C→G noncoding (35/85 nt) tyrT ← tRNA‑Tyr
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,322,868 G→A Q27* (CAG→TAG)  trpE ← anthranilate synthase subunit TrpE
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,345,203 Δ1 bp coding (1540/1986 nt) pdeR ← cyclic di‑GMP phosphodiesterase PdeR
MC JC 1,382,928 Δ13,116 bp IS5‑mediated ycjWynaI ycjW, ycjX, ycjF, tyrR, tpx, ycjG, mpaA, ymjC, ymjD, ycjY, pgrR, mppA, ynaI
RA 1,472,163 G→A pseudogene (1645/3495 nt) ydbA → putative outer membrane protein, N‑terminal fragment
MC JC 1,494,303 Δ11 bp coding (759‑769/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,531,060 C→T intergenic (‑556/‑756) yncO ← / → ydcC protein YncO/H repeat‑associated putative transposase YdcC
RA 1,540,158 C→T A811A (GCG→GCA narZ ← nitrate reductase Z subunit alpha
RA 1,568,501 A→G F108S (TTC→TCC)  digH ← divisome localized glycosyl hydrolase
RA 1,592,212 C→T S77S (TCG→TCA hipB ← antitoxin/DNA‑binding transcriptional repressor HipB
RA 1,630,908 T→C G2G (GGA→GGG ydfI ← putative oxidoreductase YdfI
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,887 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,670,921 G→A G408E (GGG→GAG)  ynfM → putative transporter YnfM
RA 1,684,504 G→T Q82H (CAG→CAT tus → DNA replication terminus site‑binding protein
RA 1,710,681 G→A V160M (GTG→ATG)  rsxG → SoxR [2Fe‑2S] reducing system protein RsxG
RA 1,761,371 C→A A141S (GCC→TCC)  sufD ← Fe‑S cluster scaffold complex subunit SufD
RA 1,857,073 G→A L233F (CTC→TTC)  ydjI ← L‑glycero‑L‑galacto‑octuluronate‑1‑phosphate aldolase
RA 1,889,299 T→G T150P (ACC→CCC)  fadD ← long‑chain‑fatty‑acid‑‑CoA ligase
RA 1,895,329 G→A Q175Q (CAG→CAA pabB → aminodeoxychorismate synthase subunit 1
RA 1,926,213 C→T P32S (CCC→TCC)  exoX → exonuclease X
RA 1,926,324 C→T P69S (CCA→TCA)  exoX → exonuclease X
RA 1,933,994 C→T R204H (CGC→CAC)  edd ← phosphogluconate dehydratase
RA 1,935,118 C→T L399L (CTG→CTA zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
RA 1,935,208 C→T Q369Q (CAG→CAA zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
RA 1,947,914 C→T A80T (GCA→ACA)  yebC ← putative transcriptional regulator YebC
RA 1,960,121 G→A A20A (GCG→GCA argS → arginine‑‑tRNA ligase
RA 1,969,375 C→A intergenic (‑11/+8) cheR ← / ← tap chemotaxis protein methyltransferase/methyl‑accepting chemotaxis protein Tap
RA 1,977,962 G→A T79M (ACG→ATG)  flhD ← DNA‑binding transcriptional dual regulator FlhD
RA 2,010,920 C→T R107R (CGC→CGT yedL → putative acetyltransferase YedL
RA 2,011,389 A→G intergenic (+310/+1311) yedL → / ← fliE putative acetyltransferase YedL/flagellar protein FliE
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
MC JC 2,102,944 Δ39,454 bp IS5‑mediated [wbbL][udk] 36 genes
RA 2,160,741 C→T P786S (CCG→TCG)  mdtC → multidrug efflux pump RND permease subunit MdtC
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,191,105 C→T D181N (GAC→AAC)  yehC ← putative fimbrial chaperone YehC
RA 2,202,345 T→A D689E (GAT→GAA yehI → DUF4132 domain‑containing protein YehI
RA 2,247,103 C→T R477H (CGC→CAC)  lysP ← lysine:H(+) symporter
RA 2,250,944 C→T N35N (AAC→AAT nfo → endonuclease IV
RA 2,280,839 C→T Q70* (CAG→TAG)  radD → putative DNA repair helicase RadD
RA 2,325,793 C→T T346T (ACC→ACT atoE → short chain fatty acid transporter
RA 2,351,449 C→T C309Y (TGC→TAC)  glpT ← sn‑glycerol 3‑phosphate:phosphate antiporter
RA 2,388,299 T→C Q43R (CAA→CGA)  yfbN ← uncharacterized protein YfbN
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,420,652 2 bp→TG coding (32‑33/648 nt) yfcG → disulfide bond oxidoreductase YfcG
RA 2,430,737 G→A I9I (ATC→ATT cvpA ← colicin V production protein
RA 2,537,509 G→A A56A (GCG→GCA yfeK → DUF5329 domain‑containing protein YfeK
RA 2,577,284 G→A S365L (TCA→TTA)  maeB ← malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
RA 2,666,080 T→C S216P (TCC→CCC)  csiE → stationary phase‑inducible protein CsiE
RA 2,680,564 G→A P728S (CCG→TCG)  yphG ← DUF5107 domain‑containing protein YphG
RA 2,704,184 C→T intergenic (‑121/+151) rnc ← / ← lepB RNase III/signal peptidase I
RA 2,739,388 C→A E228* (GAG→TAG)  tyrA ← fused chorismate mutase/prephenate dehydrogenase
RA 2,742,238 G→A E122K (GAA→AAA)  yfiR → DUF4154 domain‑containing protein YfiR
RA 2,773,749 G→A M144I (ATG→ATA yfjW → uncharacterized protein YfjW
RA 2,789,030 C→T Q16* (CAG→TAG)  glaH → glutarate dioxygenase GlaH
RA 2,864,262 C→T S9S (TCC→TCT ygbM → putative 2‑oxo‑tetronate isomerase YgbM
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,870,068 G→A T69T (ACC→ACT umpG ← broad specificity 5'(3')‑nucleotidase and polyphosphatase
RA 2,900,293 2 bp→CT intergenic (‑20/‑298) ygcW ← / → yqcE putative deoxygluconate dehydrogenase/putative transport protein YqcE
RA 2,964,704 T→A Q151L (CAG→CTG)  thyA ← thymidylate synthase
RA 2,966,382 C→T G685R (GGG→AGG)  ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 3,028,801 G→A A71V (GCG→GTG)  ygfS ← putative electron transport protein YgfS
MC JC 3,102,359 Δ27,786 bp IS5‑mediated [trmB][yghO] 24 genes
RA 3,156,738 A→T E39V (GAA→GTA)  dkgA → methylglyoxal reductase DkgA
RA 3,160,398 G→A P250L (CCA→CTA)  ygiQ ← radical SAM superfamily protein YgiQ
RA 3,164,489 C→T L495L (CTG→CTA parC ← DNA topoisomerase IV subunit A
RA 3,166,817 G→A P301L (CCG→CTG)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,249,171 C→T A68V (GCC→GTC)  yqjC → DUF1090 domain‑containing protein YqjC
RA 3,351,722 A→T V435E (GTG→GAG)  arcB ← sensor histidine kinase ArcB
RA 3,373,610 G→A intergenic (‑34/+88) nanA ← / ← nanR N‑acetylneuraminate lyase/NanR
RA 3,408,916 C→T A437V (GCC→GTC)  panF → pantothenate:Na(+) symporter
RA 3,447,645 C→T L41L (CTG→CTA rplX ← 50S ribosomal subunit protein L24
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,504,190 G→T V47F (GTC→TTC)  frlR → DNA‑binding transcriptional regulator FrlR
RA 3,522,464 C→T R96R (CGG→CGA hofM ← DNA utilization protein HofM
RA 3,526,069 C→T V27V (GTG→GTA nudE ← ADP‑sugar diphosphatase NudE
RA 3,531,473 C→T A323T (GCG→ACG)  yhgE ← putative transport protein YhgE
RA 3,535,996 T→C M197V (ATG→GTG)  ompR ← DNA‑binding transcriptional dual regulator OmpR
RA 3,548,980 C→T G364D (GGC→GAC)  malQ ← 4‑alpha‑glucanotransferase
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,860 C→T G198D (GGC→GAC)  glpG ← rhomboid protease GlpG
RA 3,562,463 C→T L151L (CTG→TTG)  glpD → aerobic glycerol 3‑phosphate dehydrogenase
RA 3,566,305 C→T G93R (GGA→AGA)  glgP ← glycogen phosphorylase
RA 3,590,729 C→T T61T (ACG→ACA ugpA ← sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA
RA 3,629,131 C→T G136G (GGG→GGA rbbA ← ribosome‑associated ATPase
RA 3,632,121 C→T W157* (TGG→TAG)  yhiJ ← DUF4049 domain‑containing protein YhiJ
RA 3,633,986 C→T intergenic (‑1396/‑855) yhiJ ← / → yhiM DUF4049 domain‑containing protein YhiJ/inner membrane protein YhiM
RA 3,664,883 C→T noncoding (20/106 nt) gadY → small regulatory RNA GadY
RA 3,664,986 C→T *275* (TAG→TAA gadX ← DNA‑binding transcriptional dual regulator GadX
RA 3,674,975 C→T L64L (CTA→TTA)  yhjE → putative transporter YhjE
RA 3,676,176 A→G intergenic (+68/+114) yhjE → / ← yhjG putative transporter YhjE/AsmA family protein YhjG
MC JC 3,682,261 Δ34 bp coding (1154‑1187/1287 nt) dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,689,865 T→C K133E (AAA→GAA)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,715,750 C→T G215G (GGG→GGA bisC ← biotin sulfoxide reductase
RA 3,718,452 C→T G198D (GGC→GAC)  yiaF ← DUF3053 domain‑containing protein YiaF
RA 3,720,845 C→T P56S (CCC→TCC)  insJ → insertion element IS150 protein InsA
RA 3,726,824 C→T A313V (GCG→GTG)  wecH → O‑acetyltransferase WecH
JC JC 3,737,137 IS2 (+) +5 bp coding (37‑41/825 nt) bax ← putative glycoside hydrolase Bax
RA 3,749,637 C→T L136L (CTG→TTG)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,749,983 C→T S251F (TCT→TTT)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,758,589 C→T D425N (GAC→AAC)  selB ← selenocysteyl‑tRNA‑specific translation elongation factor
RA 3,777,211 C→T intergenic (+184/‑188) yibL → / → lldP DUF2810 domain‑containing protein YibL/lactate/glycolate:H(+) symporter LldP
RA 3,782,634 C→T intergenic (‑72/+8) cysE ← / ← gpsA serine acetyltransferase/glycerol‑3‑phosphate dehydrogenase
RA 3,789,826 C→T G86S (GGC→AGC)  waaH ← UDP‑glucuronate:LPS(HepIII) glycosyltransferase
RA 3,790,354 C→T G331D (GGC→GAC)  tdh ← threonine dehydrogenase
RA 3,799,201 C→T A38T (GCA→ACA)  waaU ← putative ADP‑heptose:LPS heptosyltransferase 4
RA 3,872,404 C→T E199K (GAA→AAA)  dgoD ← D‑galactonate dehydratase
RA 3,895,844 C→T V256M (GTG→ATG)  adeP ← adenine:H(+) symporter
RA 3,900,993 C→T D410N (GAT→AAT)  bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,905,833 T→C E245E (GAA→GAG bglG ← transcriptional antiterminator BglG
RA 3,906,571 A→G intergenic (‑4/+282) bglG ← / ← phoU transcriptional antiterminator BglG/negative regulator of the pho regulon
RA 3,923,107 C→T A192T (GCC→ACC)  rsmG ← 16S rRNA m(7)G527 methyltransferase
RA 3,948,736 C→T A412T (GCC→ACC)  yifB ← putative magnesium chelatase YifB
RA 3,954,084 T→C S203P (TCA→CCA)  ilvD → dihydroxy‑acid dehydratase
RA 3,958,138 C→T R57C (CGT→TGT)  ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,964,147 C→T G28E (GGA→GAA)  gpp ← guanosine‑5'‑triphosphate,3'‑diphosphate phosphatase
RA 3,972,070 C→T P271S (CCG→TCG)  wecC → UDP‑N‑acetyl‑D‑mannosamine dehydrogenase
RA 3,974,173 C→T P189L (CCG→CTG)  rffH → glucose‑1‑phosphate thymidylyltransferase 2
RA 4,001,322 T→A intergenic (+48/‑104) ysgD → / → corA protein YsgD/Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
JC JC 4,002,526 IS5 (–) +4 bp coding (271‑274/381 nt) yigF ← DUF2628 domain‑containing protein YigF
RA 4,009,698 G→A A177T (GCA→ACA)  pldB → lysophospholipase L2
RA 4,012,448 T→C K123K (AAA→AAG metR ← DNA‑binding transcriptional dual regulator MetR
RA 4,012,454 Δ1 bp coding (363/954 nt) metR ← DNA‑binding transcriptional dual regulator MetR
RA 4,026,734 Δ1 bp coding (208/702 nt) fre → NAD(P)H‑flavin reductase
RA 4,045,543 C→T R449C (CGT→TGT)  yihF → DUF945 domain‑containing protein YihF
RA 4,057,040 A→G M332T (ATG→ACG)  glnA ← glutamine synthetase
RA 4,067,781 C→T G499D (GGC→GAC)  yihQ ← sulfoquinovosidase
RA 4,086,536 C→T A174V (GCC→GTC)  fdhD → sulfurtransferase for molybdenum cofactor sulfuration
RA 4,093,609 C→T D222N (GAT→AAT)  rhaD ← rhamnulose‑1‑phosphate aldolase
RA 4,094,498 C→T intergenic (‑226/+225) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,096,858 C→T L197L (CTG→CTA rhaB ← rhamnulokinase
JC JC 4,123,923 IS2 (–) +5 bp coding (530‑534/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
RA 4,152,733 G→A I122I (ATC→ATT ppc ← phosphoenolpyruvate carboxylase
RA 4,166,486 C→T intergenic (+201/‑173) murI → / → rrsB glutamate racemase/16S ribosomal RNA
RA 4,185,812 G→A E155K (GAA→AAA)  rpoC → RNA polymerase subunit beta'
RA 4,221,743 G→A R249W (CGG→TGG)  arpA ← regulator of acetyl CoA synthetase
RA 4,222,690 G→A intergenic (‑203/+114) arpA ← / ← iclR regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,317,811 G→A L29F (CTC→TTC)  phnL ← methylphosphonate degradation complex subunit PhnL
RA 4,360,480 G→A T485T (ACC→ACT cadC ← DNA‑binding transcriptional activator CadC
RA 4,372,088 A→G E355G (GAA→GGA)  groL → chaperonin GroEL
RA 4,423,020 G→A A59T (GCG→ACG)  ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE
RA 4,561,138 T→C F70S (TTT→TCT)  kptA → RNA 2'‑phosphotransferase
RA 4,620,534 A→T H311L (CAC→CTC)  deoB → phosphopentomutase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196325 1211422 15098 19 [15] [12] 17 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270 776 87 [0] [0] 112 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423919–3424234 3424389–3424238 5–471 17 [13] [16] 17 rrlD 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)52 (0.560) 43/598 0.8 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 382183 NA (NA)noncoding (924/1331 nt) IS2 repeat region
* ? NC_000913 382179 =NA (NA)23 (0.250) 22/594 2.3 NA noncoding (920/1331 nt) IS2 repeat region
?NC_000913 = 382590 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 1397238NA (NA)87 (0.940) 61/598 0.2 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)112 (1.210) 77/598 0.1 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 = 2102943NA (NA)48 (0.520) 36/598 1.1 34.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 2256027 = 92 (0.990)intergenic (+13/+58) yeiL/psuT DNA‑binding transcriptional activator YeiL/putative pseudouridine transporter