Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,004,748	C→T	intergenic (+91/‑20)	ycbF → / → pyrD	putative fimbrial chaperone YcbF/dihydroorotate dehydrogenase, type 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,004,748	0	C	T	100.0%	212.7 / NA	58	intergenic (+91/‑20)	ycbF/pyrD	putative fimbrial chaperone YcbF/dihydroorotate dehydrogenase, type 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/35);  total (23/35)

ACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATCGGTACCGTCACGCCACGT  >  NC_000913/1004453‑1005037                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                  aCAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCggg                                                                                                                                                                                                                                                                                               >  2:687583/1‑300 (MQ=255)       cACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACCTAGTACGACCCAGGTTTGCACATTGGGAATCCa                                                                                                                                                                                                                                                                                         >  2:91139/1‑300 (MQ=255)           aaCCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGgag                                                                                                                                                                                                                                                                                     <  1:110249/300‑1 (MQ=255)             cccAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGagag                                                                                                                                                                                                                                                                                   >  2:418858/1‑300 (MQ=255)                aaCGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCTTTAAGNAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGNTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTc                                                                                                                                                                                                                                                                                <  2:655754/300‑1 (MQ=255)                                    tGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAANGATTACGGCGGGTTGAGTGCAAAGAANGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCAttcgttcg                                                                                                                                                                                                                                                            >  2:491066/1‑300 (MQ=255)                                                gtgtAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGNTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTNTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGAGAATCCAGGAGAGTTCATGTACTAACCCTTCGTTCGTAAAGCCCtttt                                                                                                                                                                                                                                                >  2:564623/1‑300 (MQ=255)                                                gtgtAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAgtcttttt                                                                                                                                                                                                                                                >  2:8533/1‑293 (MQ=255)                                                      aTGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGNTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCt                                                                                                                                                                                                                                          >  1:525461/1‑300 (MQ=255)                                                                gTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCagag                                                                                                                                                                                                                                <  1:463307/300‑1 (MQ=255)                                                                   aaGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTNGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAgcgc                                                                                                                                                                                                                             >  1:482483/1‑300 (MQ=255)                                                                      tAATTTGGTAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCAGGTCGCCTGGCGTGCGATAAATGATTACGGCGGGTTGAGTGCAGAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCt                                                                                                                                                                                                                          <  2:11245/300‑1 (MQ=255)                                                                                        gccTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAg                                                                                                                                                                                                        >  1:142130/1‑300 (MQ=255)                                                                                                 ccAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGc                                                                                                                                                                                               <  1:63171/300‑1 (MQ=255)                                                                                                     cGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTNGCGTTTTTTTTGCCGCAGGTCAATTCNCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTAGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTa                                                                                                                                                                                           >  2:484936/1‑300 (MQ=255)                                                                                                           aCAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAg                                                                                                                                                                                     <  1:225181/300‑1 (MQ=255)                                                                                                                           caagcGCACTGTTTCATGTCGCCTGGCGAGCGATAAATGATTGCGGCGGGTTGAGTGCAAAGAAGGAGTGAAATCTGCCCTGAAACAGTTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCATGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCTCTTTTCCAGCGCAATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGttttaacc                                                                                                                                                                     <  2:328831/296‑6 (MQ=255)                                                                                                                           cAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGc                                                                                                                                                                     >  1:165235/1‑300 (MQ=255)                                                                                                                           cAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGc                                                                                                                                                                     >  2:153704/1‑300 (MQ=255)                                                                                                                             aTCGCACCGTGGCATGGCGCCTGGCGGGCGAGAAATGAGTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCTCTTTTGGTCCGAACTCGCACATAATACGCCCCCTGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCAc                                                                                                                                                                   <  2:19260/300‑1 (MQ=255)                                                                                                                                     gtgtCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCNGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCgg                                                                                                                                                           <  1:480007/300‑1 (MQ=255)                                                                                                                                     gtgtCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCgg                                                                                                                                                           >  2:72354/1‑300 (MQ=255)                                                                                                                                     gtgtCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGATGCgg                                                                                                                                                           >  2:98670/1‑300 (MQ=255)                                                                                                                                     gtgtCATGTCGCCTGGCGGGCGATAAATGATNACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTNGGTCCGAACTCGCACATAATACGCCCACGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCgg                                                                                                                                                           >  2:515797/1‑300 (MQ=255)                                                                                                                                     gtggcATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGATCAAAATCTTCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCNGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCGCATGAGTTTACTTTTCAGTAATTACGCCGTATTATAGGAACGCCGTTTGAAGCACTGGTGCgg                                                                                                                                                           <  1:479358/296‑1 (MQ=255)                                                                                                                                                    gcgggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCg                                                                                                                                            <  1:707403/300‑1 (MQ=255)                                                                                                                                                       ggcgATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCACGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGaaa                                                                                                                                         >  1:363904/1‑300 (MQ=255)                                                                                                                                                                tGATTACGGCGGGTTGAGTGCAAAGGAGGAGCAAAATCTGCCCGGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAAc                                                                                                                                <  2:351583/300‑1 (MQ=255)                                                                                                                                                                   ttACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCTGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTGTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAGGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGc                                                                                                                             <  2:282667/300‑1 (MQ=255)                                                                                                                                                                        gcggGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGCAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGNCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCNTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATggg                                                                                                                        <  2:569766/300‑1 (MQ=255)                                                                                                                                                                          gggTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAACCAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCCTTTGGTCCGAACTCGCACAGAATACGCCCCTGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGGAGCCTGTTAATTGcgcgggcc                                                                                                                      <  1:654367/300‑8 (MQ=255)                                                                                                                                                                          gggTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGcc                                                                                                                      <  1:282754/300‑1 (MQ=255)                                                                                                                                                                            gTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTg                                                                                                                    >  1:194630/1‑300 (MQ=255)                                                                                                                                                                               gAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGTACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCAAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTAAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCATGACg                                                                                                                 >  2:185254/1‑300 (MQ=255)                                                                                                                                                                               gAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACg                                                                                                                 <  1:8542/300‑1 (MQ=255)                                                                                                                                                                                 gTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGtt                                                                                                               >  1:448731/1‑300 (MQ=255)                                                                                                                                                                                                                  ggTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGa                                                                              >  1:406609/1‑300 (MQ=255)                                                                                                                                                                                                                         aaaaCGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGTAACGCCGTTTGAATCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGAc                                                                       <  2:141562/300‑1 (MQ=255)                                                                                                                                                                                                                                    gTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCAt                                                            <  1:241307/300‑1 (MQ=255)                                                                                                                                                                                                                                     ttttttttGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCAtt                                                           <  1:315006/300‑1 (MQ=255)                                                                                                                                                                                                                                       ttttttGCCGCAGGTCAATTTCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGa                                                         <  2:150404/300‑1 (MQ=255)                                                                                                                                                                                                                                       ttttttGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATAGGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCNTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTCCTTTTCAGCAATTACGCCGTATTACANGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGa                                                         <  2:578709/300‑1 (MQ=255)                                                                                                                                                                                                                                              ccGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTa                                                  <  1:574572/300‑1 (MQ=255)                                                                                                                                                                                                                                                gCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAgg                                                <  1:710746/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 tgttCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGgat                               <  1:146760/297‑1 (MQ=255)                                                                                                                                                                                                                                                                 tGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGTGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGNGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACATGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGgat                               <  2:630050/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 tGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAACGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGgat                               <  2:616373/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 tGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGgat                               <  1:338011/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            cGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACTCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATc                    <  2:40088/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            cGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATc                    <  1:142961/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            cGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACNTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCNTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATc                    <  2:576285/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            cGCACATAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGAGCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATc                    <  1:128077/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              cacaTAATACGCCCCCGGTTTGCACATCGGGAATCCAGGATAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATCgg                  >  1:563502/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               acaTAATACGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTANTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGAAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATCGGt                 <  1:528085/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  taataCGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTNGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGNGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATCGGTAcc              >  2:663018/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    ataCGCCCCCGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATCGGTACCGt            <  2:42674/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         cccccGGTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATCGGTACCGTCACGc       <  1:153358/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              ggTTTGCACATCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTAAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATCGGTACCGTCAAGCCACGt  >  1:162991/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                  ACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCCGGTTTGCACACCGGGAATCCAGGAGAGTTCATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAGTTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAGAAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTGGCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGATCGATCGAGATCGGTACCGTCACGCCACGT  >  NC_000913/1004453‑1005037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑