Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	798,989	C→T	S135L (TCA→TTA)	pgl →	6‑phosphogluconolactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	798,989	0	C	T	100.0%	148.8 / NA	41	S135L (TCA→TTA)	pgl	6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/21);  total (20/21)

TGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTGGGCGGCTG  >  NC_000913/798707‑799282                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                      tggtggTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGc                                                                                                                                                                                                                                                                                      <  1:652725/300‑1 (MQ=255)    tggtCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCtgt                                                                                                                                                                                                                                                                                   >  2:456386/1‑300 (MQ=255)         aGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGtt                                                                                                                                                                                                                                                                              <  1:305956/300‑1 (MQ=255)                    aCGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTaaa                                                                                                                                                                                                                                                                   >  1:290896/1‑300 (MQ=255)                     cGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAg                                                                                                                                                                                                                                                                  >  1:640147/1‑300 (MQ=255)                              tATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGc                                                                                                                                                                                                                                                         <  1:351248/300‑1 (MQ=255)                                tGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCAt                                                                                                                                                                                                                                                       <  1:357240/300‑1 (MQ=255)                                     gtgtTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCATGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCTGCATTAAAGCAGGATCGCATTTGcc                                                                                                                                                                                                                                                  <  2:346400/300‑1 (MQ=255)                                              cTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGGGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACgg                                                                                                                                                                                                                                         <  1:303391/300‑1 (MQ=255)                                                gAGTTTCGCGTCCTTGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGTCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTc                                                                                                                                                                                                                                       <  2:134208/300‑1 (MQ=255)                                                gAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTCCCATTCCGCCAATCTCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGAAGGATCGCATTTGCCTGTTTACGGTc                                                                                                                                                                                                                                       >  2:433149/1‑300 (MQ=255)                                                              ggCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGTCTGTTTACGGTCAGCGATGATGGTCa                                                                                                                                                                                                                         >  2:46880/1‑300 (MQ=255)                                                                      gtatcgCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTAGTgg                                                                                                                                                                                                                 >  2:263595/1‑300 (MQ=255)                                                                           gCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAg                                                                                                                                                                                                            >  2:280298/1‑300 (MQ=255)                                                                              ccgtaCGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGAc                                                                                                                                                                                                         <  1:238077/296‑1 (MQ=255)                                                                              ccGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGAc                                                                                                                                                                                                         <  1:238343/300‑1 (MQ=255)                                                                                                      gCCGCAGAGTCTGCGCTGCCGTGGAGTCCTACGCATATTTCCACCGAGCACCAGGGGCAGGTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCATTGGGCGTCGTCGATGTGGTCGATGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGaa                                                                                                                                                                                 <  2:119002/300‑1 (MQ=255)                                                                                                       ccGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTNGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAg                                                                                                                                                                                <  1:521347/300‑1 (MQ=255)                                                                                                         gCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGTGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCTTCTTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAggg                                                                                                                                                                              <  2:35544/300‑1 (MQ=255)                                                                                                                cTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTAGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGggccggcc                                                                                                                                                                       >  2:123763/1‑300 (MQ=255)                                                                                                                  gcgcTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCcg                                                                                                                                                                     >  2:307513/1‑300 (MQ=255)                                                                                                                     cTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGt                                                                                                                                                                  >  1:689613/1‑300 (MQ=255)                                                                                                                       gCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTTAAGGGGCCGGCCCGCGTCa                                                                                                                                                                >  1:99017/1‑300 (MQ=255)                                                                                                                                 ccGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGTTTCTTACNATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATtc                                                                                                                                                      <  2:529956/300‑1 (MQ=255)                                                                                                                                 ccGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGGGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATACCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTTGCGCAGGACCCTGCGGAAGTGACCACCGTTTAAGGGGCCGGCCCGCGTCATATGGTATtc                                                                                                                                                      <  2:370122/300‑1 (MQ=255)                                                                                                                                         tatTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCaaa                                                                                                                                              >  1:71951/1‑300 (MQ=255)                                                                                                                                           tttCCACCGATCACTAGGGGCAGTTTGTCTTTTTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGTAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTTAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACg                                                                                                                                            <  2:409956/300‑1 (MQ=255)                                                                                                                                                  cGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAata                                                                                                                                     <  1:309671/300‑1 (MQ=255)                                                                                                                                                                   ttGTCTTTGTAGGTTCTTACAAGGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATg                                                                                                                    <  1:698552/300‑1 (MQ=255)                                                                                                                                                                        tttGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTa                                                                                                               >  1:599074/1‑300 (MQ=255)                                                                                                                                                                              ggTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCCAGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGc                                                                                                         >  1:124048/1‑300 (MQ=255)                                                                                                                                                                                   ttACAATGCGGGTCACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGNGGGTTCCGGCATTAAAGCAGGATCGCNTTTGCCTGTTTACGGTCAGCGATGATGGTCATCTAGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGt                                                                                                    >  2:481371/1‑300 (MQ=255)                                                                                                                                                                                                                           ggCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGaa                                                            >  2:29414/1‑300 (MQ=255)                                                                                                                                                                                                                                   aGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTAACGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTAGTGGCGCAGGACCCTGCGGAAGTGACCAACGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCCAACGAACAATATGCGTATTGCGTCAATGAGCTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCa                                                    >  2:65483/1‑300 (MQ=255)                                                                                                                                                                                                                                        gCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGc                                               <  2:71777/300‑1 (MQ=255)                                                                                                                                                                                                                                           tcgtcgATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAGTATCGAATGTGTACAGAAGCTgg                                            >  2:180977/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ggTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGcc                                  >  1:170765/1‑300 (MQ=255)                                                                                                                                                                                                                                                      gTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGNGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATNCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGATCAGTGGATGTCTGGGAACTGAATGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGcag                                 >  2:575628/1‑298 (MQ=255)                                                                                                                                                                                                                                                            gggCTGGACGGTTGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAAc                           <  1:261746/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        tGCCATTCCGCCAATATCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATatgatctgtctcttatacacatctcgc               <  1:347536/300‑23 (MQ=255)                                                                                                                                                                                                                                                                                     atatCTTACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTGGGCGGCTg  <  1:235910/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                                      TGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTCCTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTGCCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCTTACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTCGATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACGCTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGTCATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCATATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTGGGCGGCTG  >  NC_000913/798707‑799282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑