Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,540,158	C→T	A811A (GCG→GCA)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,540,158	0	C	T	100.0%	184.2 / NA	51	A811A (GCG→GCA)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/24);  total (27/24)

GAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCCGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGATACTTCTGCATATACTCGTGGCCTTTGCCGGAGGAGCCAAGCAGGTTAGAGC  >  NC_000913/1539885‑1540453                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                        gagcgagGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGAACGCTGCAGAAAGCGGATGCATAAACGGATg                                                                                                                                                                                                                                                                               >  2:3029/1‑300 (MQ=255)       ggTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCNCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCNACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATc                                                                                                                                                                                                                                                                         >  2:574349/1‑300 (MQ=255)        gTAAAGCGTTCGTACGTAGCAGGTTAGTCGCGCTCCACCTCCACAATATTCTCCGCTGTTTTGCCTGGAATCAGATCGCATTTACCTTTGCGCCATTCGAGCACGTCACACGGCTGCGAGAGCTCTGCCAGAGAGTCATGCAGTAGGGTTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACACACACTTGCGAAAATGCTTTGTCGATACCTTTGTAGATTTCCCATTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATcc                                                                                                                                                                                                                                                                        <  2:72858/300‑1 (MQ=255)         tAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCg                                                                                                                                                                                                                                                                       <  2:146978/300‑1 (MQ=255)               gTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGgtgt                                                                                                                                                                                                                                                                 >  1:620102/1‑300 (MQ=255)                    tACGTAGCATGGTAGTCGCGCTCCACCGCCACAATATTCGGCTCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCata                                                                                                                                                                                                                                                            <  2:7281/300‑1 (MQ=255)                      cGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATc                                                                                                                                                                                                                                                          <  2:645838/300‑1 (MQ=255)                        tAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGt                                                                                                                                                                                                                                                        >  2:358351/1‑300 (MQ=255)                        tAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGt                                                                                                                                                                                                                                                        >  1:621534/1‑300 (MQ=255)                             gggTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCtttt                                                                                                                                                                                                                                                   >  1:166865/1‑300 (MQ=255)                             gggTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCAGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTTATATCGTCtttt                                                                                                                                                                                                                                                   >  2:26155/1‑300 (MQ=255)                                        cTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGt                                                                                                                                                                                                                                        <  1:242073/300‑1 (MQ=255)                                                         tCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGa                                                                                                                                                                                                                       <  1:459544/300‑1 (MQ=255)                                                               cgGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACTGCTGTGAGAGCTCTGCCTGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGTAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCgg                                                                                                                                                                                                                 <  2:291357/300‑1 (MQ=255)                                                                 gTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGgag                                                                                                                                                                                                               >  2:239058/1‑300 (MQ=255)                                                                 gTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCACCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGNCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGgag                                                                                                                                                                                                               <  2:637803/300‑1 (MQ=255)                                                                                   tCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACNCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGAc                                                                                                                                                                                             <  1:526202/300‑1 (MQ=255)                                                                                   tCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCGTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGAc                                                                                                                                                                                             <  1:630723/300‑1 (MQ=255)                                                                                        ttCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGNACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCg                                                                                                                                                                                        <  1:474258/300‑1 (MQ=255)                                                                                        ttCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCg                                                                                                                                                                                        <  1:695346/300‑1 (MQ=255)                                                                                            ccTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAg                                                                                                                                                                                    >  1:102227/1‑300 (MQ=255)                                                                                                   gcCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCATTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTGTGTAGATTTCCCATTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTGTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCg                                                                                                                                                                             <  1:173737/300‑1 (MQ=255)                                                                                                               cTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAATGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGt                                                                                                                                                                 >  2:24560/1‑300 (MQ=255)                                                                                                               cTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATTGGAGAACAGGCAGGTACCGGACATGTGGAAGTCGAGCGTCAACAGCAGGt                                                                                                                                                                 >  2:32317/1‑300 (MQ=255)                                                                                                               cTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGt                                                                                                                                                                 >  2:618209/1‑300 (MQ=255)                                                                                                               cTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGACAGGTACTGGCAATGCGGAAGTCGAGCGTCACCCGCAGGt                                                                                                                                                                 >  2:366708/1‑300 (MQ=255)                                                                                                               cTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCAAAGATGAACCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATACACGGATGCATATCCGAGGTGTTCATACCGTCTTTTTCGTACCAGGTGGCGGTAGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGt                                                                                                                                                                 >  2:366798/1‑300 (MQ=255)                                                                                                                 tCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCg                                                                                                                                                               >  1:429094/1‑300 (MQ=255)                                                                                                                        gCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGNACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGNGCTTGc                                                                                                                                                        >  2:596851/1‑300 (MQ=255)                                                                                                                        gCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGc                                                                                                                                                        >  1:393129/1‑300 (MQ=255)                                                                                                                        gCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCAACAGCAGGTCGAGCTTGc                                                                                                                                                        >  1:648749/1‑300 (MQ=255)                                                                                                                         cTGTGAGAGCTCTGCCGGAGGGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACACACACTTGCGAAAATGCTTTGTCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAAAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGcc                                                                                                                                                       <  2:287623/300‑1 (MQ=255)                                                                                                                         cTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGcc                                                                                                                                                       <  1:152653/300‑1 (MQ=255)                                                                                                                               gagCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCCCGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAAt                                                                                                                                                 <  2:616065/300‑1 (MQ=255)                                                                                                                                                 aTGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGNCCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCNTACCAGGTGGCGGTGGGCCGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCCCCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTc                                                                                                                               >  2:561567/1‑300 (MQ=255)                                                                                                                                                    cAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGAc                                                                                                                            >  1:37660/1‑300 (MQ=255)                                                                                                                                                            gTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCTTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCg                                                                                                                    >  1:131470/1‑300 (MQ=255)                                                                                                                                                                         gTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATANACGGATGCATATCCGAGGTGTTCATAGCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACNATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCGTTCAATCGCTGCAGTTTGCCACTCGCCTTCTTCCGGTTTGATCCCGTc                                                                                                       >  2:577045/1‑300 (MQ=255)                                                                                                                                                                            ggTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCt                                                                                                    >  1:579640/1‑300 (MQ=255)                                                                                                                                                                                     gCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAg                                                                                           >  1:708096/1‑300 (MQ=255)                                                                                                                                                                                      ccAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCTGTTTGATCCCGTCGCTGGCACCGAGt                                                                                          <  1:659224/300‑1 (MQ=255)                                                                                                                                                                                               cccACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGNCATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGccc                                                                                 >  1:484363/1‑300 (MQ=255)                                                                                                                                                                                                 cACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTg                                                                               >  1:443852/1‑300 (MQ=255)                                                                                                                                                                                                     cacaCTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGCAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATc                                                                           <  2:429569/300‑1 (MQ=255)                                                                                                                                                                                                                           cGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGAt                                                     <  1:546718/300‑1 (MQ=255)                                                                                                                                                                                                                                          tttCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGATACTTCTGCATATACt                                      <  1:269924/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gTCGCTGCGTGATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGATACTTCTGCATATACTCGTGGcc                               <  2:45600/300‑1 (MQ=255)                                                                                                                                                                                                                                                       gCGTGATTCCCACGCCGGATCGACCGCTACAGAAAGCGGATGAATAAGCGGATGCATACCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTNGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGATACTTCTGCATATACTCGTGGCCTTTGcc                         <  2:177936/300‑1 (MQ=255)                                                                                                                                                                                                                                                           gATTCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGATACTTCTGCATATACTCGTGGCCTTTGCCggag                     <  1:241105/300‑1 (MQ=255)                                                                                                                                                                                                                                                              tCCCACGCCGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGATACTTCTGCATATACTCGTGGCCTTTGCCGgaggag                  >  2:425149/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      cGGATCGACCGCTGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGTNCGAGCTTGCCTTCAATCGCTGCAGTTNGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGATACTTCTGCATATACTCGTGGCCTTTGCCGGAGGAGCCAAGCAg          <  2:466810/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              ccgctgCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGATACTTCTGCATATACTCGTGGCCTTTGCCGGAGGAGCCAAGCAGGTTAGAgc  <  2:206569/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                        GAGCGAGGTAAAGCGTTCATACGTAGCAGGGTAGTCGCGCTCCACCGCCACAATATTCGGCGCGGTTTTGCCTGGAATCAGATCGCATTCGCCTTTGCGCCAGTCGAGCACTTCACACGGCTGTGAGAGCTCTGCCGGAGAGTCATGCAGTAGGGGTTGTAATACCACGTCGGTTTCTTTGCCAAGATGACCCACGCACACTTGCGAAAATGCTTTGGCGATACCTTTGTAGATTTCCCAGTCGCTGCGTGATTCCCACGCCGGATCGACCGCCGCAGAAAGCGGATGAATAAACGGATGCATATCCGAGGTGTTCATATCGTCTTTTTCGTACCAGGTGGCGGTGGGCAGAACGATATCGGAGAACAGGCAGGTACTGGACATGCGGAAGTCGAGCGTCACCAGCAGGTCGAGCTTGCCTTCAATCGCTGCAGTTTGCCACTCGACTTCTTCCGGTTTGATCCCGTCGCTGGCACCGAGTTCCTCGCCCTGAATCCCGCTTTCGGTCCCCAGCAGATACTTCTGCATATACTCGTGGCCTTTGCCGGAGGAGCCAAGCAGGTTAGAGC  >  NC_000913/1539885‑1540453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑