Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,947,914	C→T	A80T (GCA→ACA)	yebC ←	putative transcriptional regulator YebC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,947,914	0	C	T	100.0%	176.8 / NA	49	A80T (GCA→ACA)	yebC	putative transcriptional regulator YebC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/21);  total (28/21)

TCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGC  >  NC_000913/1947619‑1948185                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                tcttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCGGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATAATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGtatca                                                                                                                                                                                                                                                                             <  1:132110/300‑1 (MQ=255)   ttcCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCAGCCAGGACCGTAACCTTAGTAGATGATGGTTTCCATGTTTGtatcatc                                                                                                                                                                                                                                                                           >  2:462326/1‑300 (MQ=255)     cccATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCAGCCAGGACCGTAACCTTCGTAGACGATGGTTTCCATGTTTGtatcatcat                                                                                                                                                                                                                                                                         >  2:31105/1‑300 (MQ=255)             gtgtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGCAGGAGATCACGCCTTTTTTGCTGAACANATAGGCAACGGAACCATCAGTACCGANGTTACCGCCACATTTGCTAAATGCATGACGCACTACAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCCTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATAATCACCgacc                                                                                                                                                                                                                                                                 >  2:469818/1‑297 (MQ=255)             gtgtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGccc                                                                                                                                                                                                                                                                 >  1:255902/1‑300 (MQ=255)             gtgtAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTAGTAGATGATGGTTTCCATGTTTGTATCATCATCAACGccc                                                                                                                                                                                                                                                                 >  2:112001/1‑300 (MQ=255)               ttaggtaTCAATCGCGCCGTCATCATAGGTCACGACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCGCGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCcac                                                                                                                                                                                                                                                               <  2:225636/294‑1 (MQ=255)               gtcgACATCAATCGCGCCGTCATCATCGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTAACGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGCCAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCcac                                                                                                                                                                                                                                                               >  1:96181/4‑300 (MQ=255)                        aaTCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCNGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGc                                                                                                                                                                                                                                                      >  2:637743/1‑300 (MQ=255)                             cgcCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTg                                                                                                                                                                                                                                                 <  1:128510/300‑1 (MQ=255)                             cgcCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCANGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCTAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTg                                                                                                                                                                                                                                                 <  2:533892/300‑1 (MQ=255)                                cGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTgcgc                                                                                                                                                                                                                                              <  1:139737/300‑1 (MQ=255)                                cGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTgcgc                                                                                                                                                                                                                                              <  2:545381/300‑1 (MQ=255)                                          ggTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAgtgt                                                                                                                                                                                                                                    <  2:549604/300‑1 (MQ=255)                                          ggTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACAGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAgtgt                                                                                                                                                                                                                                    <  1:411605/300‑1 (MQ=255)                                               caACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCCTTATGGTGGCTTCATCGCTTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCATGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTAGCATCATCACCGCCCACACCACATGCAATTGCGCGGTTCAGTGTGTCAc                                                                                                                                                                                                                               <  2:95907/300‑1 (MQ=255)                                                   gTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTANCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGt                                                                                                                                                                                                                           <  1:499085/300‑1 (MQ=255)                                                   gTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTACATGTTTGNATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTNACGGGt                                                                                                                                                                                                                           >  2:591891/1‑300 (MQ=255)                                                   gTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCAAATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCAGACAGGACCGTAACCTTCGTAGATGCTGGTTTCCATGTTTGTATCATCATCAACGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTAACGGGt                                                                                                                                                                                                                           >  2:56016/1‑300 (MQ=255)                                                     cTTCAGCACCTGCTTCCAGTGCTGCTTACATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCAACGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTcg                                                                                                                                                                                                                         >  2:222659/1‑299 (MQ=255)                                                          gCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCAtgttg                                                                                                                                                                                                                    <  2:554203/300‑1 (MQ=255)                                                               tGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGTGCGGTTCAGTGTGTCACGGGTCATGTCGTTAGa                                                                                                                                                                                                               >  2:314993/1‑300 (MQ=255)                                                                gCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTANCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGAc                                                                                                                                                                                                              <  2:633874/300‑1 (MQ=255)                                                                gCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGAc                                                                                                                                                                                                              >  1:599447/1‑300 (MQ=255)                                                                gCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGAc                                                                                                                                                                                                              >  2:397053/1‑300 (MQ=255)                                                                gCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCGCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGAc                                                                                                                                                                                                              <  1:663254/300‑1 (MQ=255)                                                                          ctgctTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTAt                                                                                                                                                                                                    >  1:172236/1‑300 (MQ=255)                                                                                ccATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCAccg                                                                                                                                                                                              >  2:335311/1‑300 (MQ=255)                                                                                                                                   tGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCcg                                                                                                                                           <  1:609393/300‑1 (MQ=255)                                                                                                                                    gAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCcgc                                                                                                                                          >  1:44810/1‑300 (MQ=255)                                                                                                                                         gATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTa                                                                                                                                     >  2:346307/1‑300 (MQ=255)                                                                                                                                               cAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTGCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCt                                                                                                                               >  2:448782/1‑300 (MQ=255)                                                                                                                                                    gAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTGGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGa                                                                                                                          >  1:54275/1‑300 (MQ=255)                                                                                                                                                        cATCAGTACCGAGGTTACCGCCACATTTGCTCAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCAACGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCAACGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGa                                                                                                                      >  1:572475/1‑300 (MQ=255)                                                                                                                                                             gTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTa                                                                                                                 >  1:56082/1‑300 (MQ=255)                                                                                                                                                                         ccGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTa                                                                                                     >  2:459128/1‑300 (MQ=255)                                                                                                                                                                                        aaTGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATcc                                                                                      <  1:261096/300‑1 (MQ=255)                                                                                                                                                                                        aaTGCANGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCANTCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCCCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATcc                                                                                      <  2:590977/300‑1 (MQ=255)                                                                                                                                                                                                     cTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTAc                                                                         >  1:238268/1‑300 (MQ=255)                                                                                                                                                                                                       tCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGACGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGa                                                                       <  2:677537/300‑1 (MQ=255)                                                                                                                                                                                                        cAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGAt                                                                      >  1:68326/1‑300 (MQ=255)                                                                                                                                                                                                         aGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATg                                                                     <  1:694205/300‑1 (MQ=255)                                                                                                                                                                                                                gtgcggttgcggttgTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTNAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGgtg                                                              >  1:486812/4‑300 (MQ=255)                                                                                                                                                                                                                 tacggtTGCGGTTGTTAGACAGACATTCAATCATGATTACCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCAGGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGTGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGgtgt                                                             <  2:55632/300‑1 (MQ=255)                                                                                                                                                                                                                      ttgcggttgTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTACATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGcc                                                        <  1:601967/300‑1 (MQ=255)                                                                                                                                                                                                                                        caTTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCAAGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTAAAGATTTTACCGCGCTTAGCATCCTGTGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGcc                                      >  2:269606/1‑300 (MQ=255)                                                                                                                                                                                                                                                  tgcttGCCGTGCCGCCAAGACCATAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCAAGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAatat                            >  2:346022/4‑300 (MQ=255)                                                                                                                                                                                                                                                              cgccAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGcc                >  2:354332/1‑300 (MQ=255)                                                                                                                                                                                                                                                              cgccAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTACAGATAGcc                >  1:457944/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     aCCGTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCAAGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGTAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCcctgcctg         >  2:123478/1‑300 (MQ=255)                                                                                                                                                                                                                                                                        gTAACCTTCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCTAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTc      <  1:404946/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            ccTTCTTAGATGATGTTTTCCATGTTTGTATCATCATCACCACCCACACCACGTTCAATTGCGCGGTTCAGTGTGTCACTGGTCATGTTTTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGc  <  1:270972/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            cattCGTAGATGATGGTTTCCATGTTTGTATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTATGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATACCTCCAGATAGCCCTGCCTGTTCAGGc  >  2:58118/3‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                TCTTCCCATGCAGTGTAGACATCAATCGCGCCGTCATCATAGGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTGTGTCACGGGTCATGTTGTTAGACAGTGCTTTATCCACCGCCGCACGCAGACGCGGGTTAGCGTCCGGATCGCCACCGCCCAGCTTAGCCGCGGTTACCAGCTCACGAATGATTTTAGTGAAGATTTTACCGCGCTTAGCATCCTGCGCAGCTTTACGATGTCTGGTGTTGGCCCATTTACTATGACCTGCCATAAAAATATCTCCAGATAGCCCTGCCTGTTCAGGC  >  NC_000913/1947619‑1948185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑