Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	978,478	C→T	S718L (TCA→TTA)	mukB →	chromosome partitioning protein MukB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	978,478	0	C	T	100.0%	182.6 / NA	50	S718L (TCA→TTA)	mukB	chromosome partitioning protein MukB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/20);  total (30/20)

AAGAGTTTACCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTCACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAG  >  NC_000913/978184‑978773                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        aaGAGTTTACCTCCAGCCAGGACGTCACCGAATATCTGCAACGGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAgg                                                                                                                                                                                                                                                                                                    <  2:345904/300‑1 (MQ=255)                    ggACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAgg                                                                                                                                                                                                                                                                                 <  1:54456/300‑1 (MQ=255)                           aCCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGNGGAGCGTTTTGGTGGTGTGCTGCTGTNAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGAcc                                                                                                                                                                                                                                                                          <  2:487397/300‑1 (MQ=255)                           aCCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGAcc                                                                                                                                                                                                                                                                          <  1:417275/300‑1 (MQ=255)                                    cTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGAACGATTGCCCg                                                                                                                                                                                                                                                                 >  1:638160/1‑300 (MQ=255)                                          cAGTTGCTGGAGCGTGAGCGGGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTAAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCNGATCTGTTACAGGTAACTGAACACCTGGAAGGCCTGACCGATTGCCCNGAAGac                                                                                                                                                                                                                                                           >  2:584586/1‑299 (MQ=255)                                          cAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCNGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCNGAAGAt                                                                                                                                                                                                                                                           >  2:583897/1‑300 (MQ=255)                                            gTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGAtct                                                                                                                                                                                                                                                         <  1:30248/300‑1 (MQ=255)                                             ttGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGAtctc                                                                                                                                                                                                                                                        <  1:276206/300‑1 (MQ=255)                                                                   cGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCACGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGc                                                                                                                                                                                                                                  >  2:336730/1‑300 (MQ=255)                                                                      ttgttgAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGt                                                                                                                                                                                                                               <  1:347688/300‑1 (MQ=255)                                                                                    gAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGc                                                                                                                                                                                                                 >  1:124964/1‑300 (MQ=255)                                                                                             gcgcgcAAAAACGCCGTCGATTAAGAGATCGAACGTTTAAGCCAGCCTGTCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGc                                                                                                                                                                                                        <  2:18065/300‑1 (MQ=255)                                                                                               gcgcAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGt                                                                                                                                                                                                      <  1:293818/300‑1 (MQ=255)                                                                                                             tCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTCTCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGaaa                                                                                                                                                                                        >  2:217791/1‑300 (MQ=255)                                                                                                               gATGAAGAGATCGAACGTTTACGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGCTTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGCCAGCGCGTTCAGCGTTAATGAGCTGGaaaaa                                                                                                                                                                                      >  2:611089/1‑300 (MQ=255)                                                                                                               gATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGaaaaa                                                                                                                                                                                      >  1:645607/1‑300 (MQ=255)                                                                                                                 tGAAGAGATCGAACGTTTAAGCCAGCCTGGCTGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGTAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGc                                                                                                                                                                                    <  2:325631/300‑1 (MQ=255)                                                                                                                              cGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGaaa                                                                                                                                                                       >  1:307047/1‑300 (MQ=255)                                                                                                                              cGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGACGAGCTGGAAAAAGCGGCAGTGGTGaaa                                                                                                                                                                       >  2:303917/1‑300 (MQ=255)                                                                                                                               gTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGaaaa                                                                                                                                                                      >  1:285143/1‑300 (MQ=255)                                                                                                                                        agccTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGTGGTAGTGGTGAAAATCGCCGATc                                                                                                                                                             >  2:145449/1‑300 (MQ=255)                                                                                                                                                    ctGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGtt                                                                                                                                                 >  2:377118/1‑300 (MQ=255)                                                                                                                                                      gAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGTTGTCAGGAATTTATGACGACGTTAGCCTGGAAGATGTGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGGTCTGTTAAAGGTAACCGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGCCATTCGATCACCACGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATTGCCGATTGTCAGTGGCGTTAt                                                                                                                                               >  2:246800/1‑300 (MQ=255)                                                                                                                                                              gCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCACGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGttt                                                                                                                                       >  2:304050/1‑300 (MQ=255)                                                                                                                                                                    gAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGa                                                                                                                                 <  1:147284/300‑1 (MQ=255)                                                                                                                                                                             ggCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTAAGCGATGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATNCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCt                                                                                                                        >  1:491886/1‑300 (MQ=255)                                                                                                                                                                                      ttttGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCg                                                                                                               >  1:536741/1‑300 (MQ=255)                                                                                                                                                                                                                tttATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCTTTGATGAGCTGGAAAAAGCGGTAGTGTTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCTTATTGaaa                                                                                     <  2:213194/300‑1 (MQ=255)                                                                                                                                                                                                                tttATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGaaa                                                                                     <  1:325968/300‑1 (MQ=255)                                                                                                                                                                                                                         acgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATg                                                                            >  2:104630/1‑300 (MQ=255)                                                                                                                                                                                                                         acgTTAGCCTGGAAGATGCGCCGTACTTCTAAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATg                                                                            >  2:248900/1‑300 (MQ=255)                                                                                                                                                                                                                          cgTTAGCCTGGAAGATGTGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGTAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGc                                                                           <  2:277580/300‑1 (MQ=255)                                                                                                                                                                                                                          cgTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCAGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGc                                                                           <  1:69980/300‑1 (MQ=255)                                                                                                                                                                                                                              agcatGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCAGATCGTCAGTGGCGTTATTCACGTTTCCAGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAg                                                                       >  2:349369/5‑300 (MQ=255)                                                                                                                                                                                                                              aGCCTGGAAGATGCGCCGTACTTCTAAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTACCGGAAGTGCCGCTGTTTGGTCGTGCTGAGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAg                                                                       >  2:149375/1‑300 (MQ=255)                                                                                                                                                                                                                              aGCCTGGAAAATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGNGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCAGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAg                                                                       >  1:519173/1‑300 (MQ=255)                                                                                                                                                                                                                                        aTGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGAGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGANAGCGTGTTCAGCGTTGATGAGCTGGANAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGc                                                             <  2:471331/300‑1 (MQ=255)                                                                                                                                                                                                                                             ccGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCATTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTcc                                                        <  1:685203/300‑1 (MQ=255)                                                                                                                                                                                                                                             ccGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTcc                                                        >  2:413189/1‑300 (MQ=255)                                                                                                                                                                                                                                              cGTACTTCTCAGCGCTGTATTGCCCGTCACGCCACGCCATCTTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCNGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGANAGCCTCCATGCCGAGCGTGAAGTGCTTTCCg                                                       <  2:511365/300‑1 (MQ=255)                                                                                                                                                                                                                                                    tctcAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGCAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCt                                                 >  2:104224/1‑300 (MQ=255)                                                                                                                                                                                                                                                            cTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACg                                         >  1:39929/1‑300 (MQ=255)                                                                                                                                                                                                                                                              gTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGct                                       >  1:168950/1‑300 (MQ=255)                                                                                                                                                                                                                                                              gTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCAGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGct                                       >  1:346425/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   gCCCGTCACGCCACGCCATCGTGGTGCCAGANCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGNGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCt                                  >  2:505317/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         cacgccacgcCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCTGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATg                            <  2:379910/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         cacgccacgcCATCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATg                            <  1:184269/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  ccaTCGTGGTGCCAGATCTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTACCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGaaaa                   >  2:289411/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   cTGTTACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAg  >  1:325424/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                        AAGAGTTTACCTCCAGCCAGGACGTCACCGAATATCTGCAACAGTTGCTGGAGCGTGAGCGAGAGGCGATTGTTGAACGCGATGAAGTGGGCGCGCGCAAAAACGCCGTCGATGAAGAGATCGAACGTTTAAGCCAGCCTGGCGGCTCTGAAGATCAGCGTCTGAACGCGCTGGCGGAGCGTTTTGGTGGTGTGCTGCTGTCAGAAATTTATGACGACGTTAGCCTGGAAGATGCGCCGTACTTCTCAGCGCTGTATGGCCCGTCACGCCACGCCATCGTGGTGCCAGATCTGTCACAGGTAACTGAACACCTGGAAGGCTTGACCGATTGCCCGGAAGATCTCTATCTGATCGAAGGAGATCCGCAGTCATTCGATGACAGCGTGTTCAGCGTTGATGAGCTGGAAAAAGCGGTAGTGGTGAAAATCGCCGATCGTCAGTGGCGTTATTCACGTTTCCCGGAAGTGCCGCTGTTTGGTCGTGCTGCGCGTGAAAGCCGTATTGAAAGCCTCCATGCCGAGCGTGAAGTGCTTTCCGAACGCTTCGCCACGCTCTCCTTTGATGTACAGAAAACTCAGCGTCTGCATCAG  >  NC_000913/978184‑978773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑