Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	148,502	G→A	I98I (ATC→ATT)	panC ←	pantothenate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	148,502	0	G	A	98.2%	196.9 / NA	57	I98I (ATC→ATT)	panC	pantothenate synthetase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (26/30);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTCCATACGCAGGCGGCGAATTTGCTGACGCAGCAGCGGCAGGGTTTCGATA  >  NC_000913/148228‑148787                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                              tACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGaaaa                                                                                                                                                                                                                                                                      >  1:181865/1‑300 (MQ=255)         cTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATcc                                                                                                                                                                                                                                                              >  1:447002/1‑300 (MQ=255)                       gTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCGTTTTGCGGATCAGCGCAAGTTGCTGAAAATCTTTTTCAACGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCAAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACgtttgtt                                                                                                                                                                                                                                                >  2:126016/1‑300 (MQ=255)                       gTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACgtttgtt                                                                                                                                                                                                                                                >  1:662474/1‑300 (MQ=255)                                       aTTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGtcc                                                                                                                                                                                                                                <  1:402546/300‑1 (MQ=255)                                       aTTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTAGCAGtcc                                                                                                                                                                                                                                >  1:551834/1‑300 (MQ=255)                                               acCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGNACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCaa                                                                                                                                                                                                                        <  1:519736/300‑1 (MQ=255)                                                       tctcAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGCAGGGGCGAAANCTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTg                                                                                                                                                                                                                >  2:523505/1‑300 (MQ=255)                                                        ctcAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGNAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCTAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTgg                                                                                                                                                                                                               <  2:160220/300‑1 (MQ=255)                                                         tcAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGa                                                                                                                                                                                                              <  1:435506/300‑1 (MQ=255)                                                         tcAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCNGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGa                                                                                                                                                                                                              <  1:478524/300‑1 (MQ=255)                                                                cGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCNAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACNTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAg                                                                                                                                                                                                       <  2:567232/300‑1 (MQ=255)                                                                       cATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATc                                                                                                                                                                                                <  1:247277/300‑1 (MQ=255)                                                                       cATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATc                                                                                                                                                                                                <  2:425967/300‑1 (MQ=255)                                                                          atCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCAGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTc                                                                                                                                                                                             >  2:378639/1‑300 (MQ=255)                                                                              gCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCggg                                                                                                                                                                                         <  1:346534/300‑1 (MQ=255)                                                                              gCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCggg                                                                                                                                                                                         <  2:250987/300‑1 (MQ=255)                                                                               cAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGc                                                                                                                                                                                        <  1:63696/300‑1 (MQ=255)                                                                                       ttGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAAc                                                                                                                                                                                <  2:354975/300‑1 (MQ=255)                                                                                                           cTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAAt                                                                                                                                                            <  1:582053/300‑1 (MQ=255)                                                                                                                       ttCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTgacgacgac                                                                                                                                                <  1:409615/300‑1 (MQ=255)                                                                                                                         cACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACca                                                                                                                                              >  2:645964/1‑300 (MQ=255)                                                                                                                             gAAGCATGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATc                                                                                                                                          <  1:159621/300‑1 (MQ=255)                                                                                                                              aaGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCg                                                                                                                                         >  2:389400/1‑300 (MQ=255)                                                                                                                                      gATGTCCGGCTGGACCAGGTTGAACAGCTTGCTTACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGc                                                                                                                                 <  2:48154/300‑1 (MQ=255)                                                                                                                                       aTGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAANTGTCCAGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGNTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCt                                                                                                                                >  2:659930/1‑300 (MQ=255)                                                                                                                                           ccGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTgg                                                                                                                            >  1:354596/1‑300 (MQ=255)                                                                                                                                            tggCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGTTCGAAATGCCAGGAACGTCAACGTAAGTGTTGGTTTCAGTACCATTCGGGTAAATCTCTTTTACCTAGGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAAACCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGc                                                                                                                           <  2:218034/299‑1 (MQ=255)                                                                                                                                            cGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGc                                                                                                                           <  1:567575/300‑1 (MQ=255)                                                                                                                                                 ggACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTtcgt                                                                                                                      >  2:610892/1‑300 (MQ=255)                                                                                                                                                  gACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTtcgtc                                                                                                                     <  1:382928/300‑1 (MQ=255)                                                                                                                                                      aGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGAcc                                                                                                                 >  1:580047/1‑300 (MQ=255)                                                                                                                                                      aGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCAGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGAcc                                                                                                                 >  2:441184/1‑300 (MQ=255)                                                                                                                                                           gAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCtt                                                                                                            <  1:649538/300‑1 (MQ=255)                                                                                                                                                                gcttgcttACAGTAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCTAAATGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCAGCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCatat                                                                                                       <  2:700214/300‑1 (MQ=255)                                                                                                                                                                   tGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACCGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGc                                                                                                    <  1:224977/300‑1 (MQ=255)                                                                                                                                                                       gaccaTAGTCGAAACGCCGCGAAAATGTCCAGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATc                                                                                                >  1:640251/5‑300 (MQ=255)                                                                                                                                                                              gTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAgg                                                                                         <  1:329973/300‑1 (MQ=255)                                                                                                                                                                                     ctccgcGAAAATGCCCCGGACGGCTGGCACCTTCCAGCATGTTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCa                                                                                  <  2:252937/298‑1 (MQ=255)                                                                                                                                                                                     cgccgcGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCa                                                                                  <  1:120254/300‑1 (MQ=255)                                                                                                                                                                                            aaaaTGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAgg                                                                           >  1:669418/1‑300 (MQ=255)                                                                                                                                                                                                tGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCAcc                                                                       <  1:716544/300‑1 (MQ=255)                                                                                                                                                                                                tGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGAGAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGGTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCAcc                                                                       <  1:176709/300‑1 (MQ=255)                                                                                                                                                                                                     cGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAgcgc                                                                  <  1:385058/300‑1 (MQ=255)                                                                                                                                                                                                           gCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGTTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCAcgcg                                                            <  1:434850/300‑1 (MQ=255)                                                                                                                                                                                                                  ccTTCCAGCATGGTCGAAAGGCCATGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTANGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCt                                                     <  2:620295/300‑1 (MQ=255)                                                                                                                                                                                                                  ccTTCCAGCATGGTCGAAAGGCCAGGAACGTNAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCNGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGGTTGCCt                                                     >  2:498914/1‑300 (MQ=255)                                                                                                                                                                                                                    ttCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCNGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTc                                                   >  2:169933/1‑300 (MQ=255)                                                                                                                                                                                                                    ttCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTc                                                   >  1:709319/1‑300 (MQ=255)                                                                                                                                                                                                                              gTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTAGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTCCATACGCAgg                                         >  1:209791/1‑300 (MQ=255)                                                                                                                                                                                                                                     ggCCAGGAACGTCAACGTAAGTGTGGGTTTCCGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTCCGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATCACGAGCAAGATCTTCCGGGCGGTCGAACCGCATCAGGTTAACGAAAATACTGACGACGACCACATCGGCGAGGGCTTTGGCTTCGTCGAACAGCCTAATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCAAAGCGCTTGCATTACATACGCAggcggcaaa                                  >  2:143509/1‑297 (MQ=255)                                                                                                                                                                                                                                                   aCGTAAGTGTGGGTTTCAGTACCGTTCGGGTAACTCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTCAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGCCCACATCGGAGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTCCATACGCAGGCGGCGAATTTGCTGACgcagc                    >  1:610881/1‑300 (MQ=255)                                                                                                                                                                                                                                                   aCGTAAGTGTGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATCGCAATCGTGCAGGTTACCCATGGTAGGNACCAGCGCCACGCGCTTGCCTTCCATACGCAGGCGGCGAATTTGCTGACgcagc                    >  2:520534/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gtgtGGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTCCATACGCAGGCGGCGAATTTGCTGACGCAGCAGCGGc              >  1:429677/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gtgGGTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTCCATACGCAGGCGGCGAATTTGCTGCCGCAGCAGCGGccg            >  1:34116/1‑298 (MQ=255)                                                                                                                                                                                                                                                              ggTTTCAGTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCAATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTCCATACGCCGGCGGCGAATTTGCTGACGCAGCAGCGGCAGGGt         >  1:599410/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     gTACCGTTCGGGTAAATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGAACAGCTTCATATGGCCATCGTGTAGGCTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTCCATACGCAGGCGGCGAATTTGCTGACGCAGCAGCGGCAGGGTTTCGATa  >  2:48097/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                              TACGGGAACTTAGCGCCAGACCGTCTTTGGCGCGCATAATTGGCACACCGACAATCTCAATATCGAAGCCCATATCGGCAACCATTTTGCGGATCAGCGCCAGTTGCTGAAAATCTTTTTCACCGAAGCAGGCGATGTCCGGCTGGACCAGGTTGAACAGCTTGCTGACAATAGTCGAAACGCCGCGAAAATGTCCCGGACGGCTGGCACCTTCCAGCATGGTCGAAAGGCCAGGAACGTCAACGTAAGTGTGGGTTTCAGTACCGTTCGGGTAGATCTCTTTTACCGAAGGGGCGAAAACTAAATCCACTTTACGTTTGTTTAGCTTCTCGCAGTCCTCCTGCAAGGTCCGTGGATAACGAGCCAGATCTTCCGGGCGGTCGAACTGCATCGGGTTAACGAAAATACTGACGACGACCACATCGGCGCGGGCTTTGGCTTCGTCGACCAGCTTCATATGGCCATCGTGCAGGTTACCCATGGTAGGCACCAGCGCCACGCGCTTGCCTTCCATACGCAGGCGGCGAATTTGCTGACGCAGCAGCGGCAGGGTTTCGATA  >  NC_000913/148228‑148787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑