Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,588	C→G	noncoding (35/85 nt)	tyrT ←	tRNA‑Tyr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,588	0	C	G	100.0%	250.2 / NA	65	noncoding (35/85 nt)	tyrT	tRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (29/36);  total (29/36)

ACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGA  >  NC_000913/1287293‑1287875                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                 tctggagcgtaccggtggagtatctgacacgtaagtaccagctggtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTc                                                                                                                                                                                                                                                                                              <  1:616955‑M2/46‑1 (MQ=255)       gcgtaccggtggagtatctgacacgtaagtaccagctggtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTcc                                                                                                                                                                                                                                                                                        >  1:233307‑M2/249‑300 (MQ=255)          ctgcgagatgtgtataagagacagagtaccagctggtgcgcgctcgtcactacgcgaattttagttcccctagaccctgcttcggaggggtttttttatgggcacggtgtggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtcaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTCGCGTCTGCTCCCtt                                                                                                                                                                                                                                                                                     >  1:698532‑M2/246‑300 (MQ=255)              ggtggagtatctgacacgtaagtaccagctggtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGc                                                                                                                                                                                                                                                                                 <  1:65536‑M2/59‑1 (MQ=255)              ggtggagtatctgacacgtaagtaccagctggtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTAGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGc                                                                                                                                                                                                                                                                                 <  1:236950‑M2/59‑1 (MQ=255)                       tctgacacgtaagtaccagctggtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgccctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtcacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGa                                                                                                                                                                                                                                                                        >  2:281453‑M2/233‑300 (MQ=255)                                   gtaccagctggtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgcaatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAATCTTCGAAGTAGATGACGGCAGATTTAGAGTCTGCTCACTTTGGCCGCTCGGGAaccccaccaagg                                                                                                                                                                                                                                                            >  2:16916‑M2/221‑297 (MQ=255)                                     accagctggtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcntatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACgggg                                                                                                                                                                                                                                                          <  1:527732‑M2/82‑1 (MQ=255)                                           tggtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccnacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGc                                                                                                                                                                                                                                                    <  1:507575‑M2/88‑1 (MQ=255)                                            ggtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCt                                                                                                                                                                                                                                                   >  1:576655‑M2/212‑300 (MQ=255)                                             gtgcgcgctcgtcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCtt                                                                                                                                                                                                                                                  <  1:190576‑M2/90‑1 (MQ=255)                                                        tcactaagcgaattttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCt                                                                                                                                                                                                                                       >  2:198078‑M2/200‑300 (MQ=255)                                                                      ttagttcacatagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCggg                                                                                                                                                                                                                         <  1:583095‑M2/115‑1 (MQ=255)                                                                              catagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtagcaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCgggg                                                                                                                                                                                                                 >  2:335230‑M2/178‑300 (MQ=255)                                                                              catagaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCgggg                                                                                                                                                                                                                 >  1:288748‑M2/178‑300 (MQ=255)                                                                                 agaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGgcgc                                                                                                                                                                                                              <  1:649324‑M2/126‑1 (MQ=255)                                                                                  gaccctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCAGCTCGGGAACCCCACCACGGGGTAGTGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGca                                                                                                                                                                                                             >  1:87771‑M2/174‑300 (MQ=255)                                                                                      ctgcttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGcatcat                                                                                                                                                                                                         <  1:266462‑M2/131‑1 (MQ=255)                                                                                         cttcggcggggtttttttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATc                                                                                                                                                                                                      <  1:136216‑M2/134‑1 (MQ=255)                                                                                                         ttatgggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTg                                                                                                                                                                                      <  1:112576‑M2/150‑1 (MQ=255)                                                                                                              ggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAg                                                                                                                                                                                 <  1:625778‑M2/155‑1 (MQ=255)                                                                                                              ggcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAg                                                                                                                                                                                 <  2:447015‑M2/155‑1 (MQ=255)                                                                                                              ggcacggggcggggtgagttgtcggatgcgcttcgcttatccgacctacatgggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAg                                                                                                                                                                                 <  2:26758‑M2/155‑1 (MQ=255)                                                                                                              gacgcggtgctgggtgagttgtcggatgcgcttcgcttatccgaccgacagtggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaagttttgattatttaagtaatcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTGTCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAg                                                                                                                                                                                 <  2:254055‑M2/155‑1 (MQ=255)                                                                                                               gcacggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGTGGGGCGCATAATATCAAATGACGCGCCGCTGTAAAgt                                                                                                                                                                                >  2:92159‑M2/145‑300 (MQ=255)                                                                                                                 acggtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAgtgt                                                                                                                                                                              <  1:685498‑M2/158‑1 (MQ=255)                                                                                                                    gtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAc                                                                                                                                                                           >  1:3544‑M2/140‑300 (MQ=255)                                                                                                                    gtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAc                                                                                                                                                                           >  2:398511‑M2/140‑300 (MQ=255)                                                                                                                    gtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCATTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAc                                                                                                                                                                           >  1:161385‑M2/140‑300 (MQ=255)                                                                                                                    gtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAAGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTtaa                                                                                                                                                                           >  2:233589‑M2/140‑299 (MQ=255)                                                                                                                    gtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTNGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCAGCTGTAAAGTGTTAc                                                                                                                                                                           >  2:631276‑M2/140‑300 (MQ=255)                                                                                                                    gtgcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCACTTNGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCAGCTGTAAAGTGTtat                                                                                                                                                                           >  2:619257‑M2/140‑299 (MQ=255)                                                                                                                      gctgggtgagttgtcggatgcgcttcgcttatccgacctacagggtaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑Gt                                                                                                                                                                        <  1:177076‑M2/163‑1 (MQ=255)                                                                                                                      gcgtggtgagttgtcagatgcgctgcgcttatccgacctacaggggaggatatggtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatgtaaggaagcgatgttggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCGTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑Gt                                                                                                                                                                        <  2:89233‑M2/163‑1 (MQ=255)                                                                                                                      gcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑Gt                                                                                                                                                                        <  1:574037‑M2/163‑1 (MQ=255)                                                                                                                      gcggggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggggccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCAGCTGTAAAGTGTTAC‑Gt                                                                                                                                                                        <  1:95808‑M2/163‑1 (MQ=255)                                                                                                                      gcgggatgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGTTCCGTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑Gt                                                                                                                                                                        <  2:452059‑M2/163‑1 (MQ=255)                                                                                                                         gggtgagttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTga                                                                                                                                                                     >  2:419462‑M2/135‑300 (MQ=255)                                                                                                                               gttgtcggatgcgcttcgcttatccgacctccaggggaggatattgtaggcccggtaaacgtggtgctatcgggcaaagttagttcggagcaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGACGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAGTGCTTTTTCCTGGCCTGCTCCCTTATCGGGAAGCGGGGGGAATCATATCAAATGACGCGACGCTGTAACGTGTTAC‑GTCgagaagaa                                                                                                                                                               >  2:263365‑M2/129‑297 (MQ=255)                                                                                                                               gttgtcggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGaaaaa                                                                                                                                                               >  1:389455‑M2/129‑300 (MQ=255)                                                                                                                                    cggatgcgcttcgcttatccgacctacatgggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatgtaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAAc                                                                                                                                                          <  1:215870‑M2/177‑1 (MQ=255)                                                                                                                                    cggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAAc                                                                                                                                                          <  1:425703‑M2/177‑1 (MQ=255)                                                                                                                                     ggatgcgcttcgcttatccgacctacaggggaggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACt                                                                                                                                                         <  1:385868‑M2/178‑1 (MQ=255)                                                                                                                                                                     ggatattgtcggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacnggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGNGTTAA‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTaaa                                                                                                                         >  2:669633‑M2/91‑300 (MQ=255)                                                                                                                                                                     ggatattgtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTaaa                                                                                                                         >  2:58979‑M2/91‑300 (MQ=255)                                                                                                                                                                            gtaggcccggtaaacgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAAcc                                                                                                                  <  1:300023‑M2/217‑1 (MQ=255)                                                                                                                                                                                         acgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCCGTTATTTTTGCt                                                                                                     >  2:143389‑M2/71‑300 (MQ=255)                                                                                                                                                                                         acgtggtgccatcgggcaaaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCt                                                                                                     >  1:70126‑M2/71‑300 (MQ=255)                                                                                                                                                                                                           aaattagctcggagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATc                                                                                   >  2:642251‑M2/53‑300 (MQ=255)                                                                                                                                                                                                                    ctgagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTAGCGATTAAAGAATAATCGTTCGATTa                                                                          >  2:100669‑M2/44‑300 (MQ=255)                                                                                                                                                                                                                     ggagtaacaggttttgattattgaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTAc                                                                         <  1:192829‑M2/258‑1 (MQ=255)                                                                                                                                                                                                                      gagtaacaggttttgattatttaaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTAcc                                                                        >  1:118538‑M2/42‑300 (MQ=255)                                                                                                                                                                                                                        ggaacatgttttgattattgaaggaagcgatggtggtgcGGGAAGGATTCGAACNTTCGAAGTCGATGACGGCAGATTTAGACTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGt                                                                      <  2:538013‑M2/261‑1 (MQ=255)                                                                                                                                                                                                                            caggttttgattatttaaggaagcgatggtggtgcTGGAAGGATTCGATCCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACNGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGANGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCATAAAc                                                                  <  2:567428‑M2/265‑1 (MQ=255)                                                                                                                                                                                                                                            aaggaagcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTACTTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTTGATTACCGTAAACAAAGAAGCGCTGTGcc                                                  >  2:319276‑M2/20‑300 (MQ=255)                                                                                                                                                                                                                                                 ggcgatattggtgcgtgAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTTGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGTGGTAATGCTTTTTACTGTCCTGCTCCCCTATCGTGAAGCTGGTCGCATCATATCAAATGACGCGCCGCTCTAAAGTTGTACTGTT‑AAAAAAGTGAACTTGTTGCGTAATGTTCATCCGTGACGAATTAAAGGTAACCAGTGGTGTTGACTGACAAGGAAAGAATAACCGTTCGATGACCGTAAACAAGGACGCGCTGTGCCAGTAc                                             <  2:683472/284‑1 (MQ=16)                                                                                                                                                                                                                                                 agcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTNGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCNGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTAc                                             <  2:655118‑M2/286‑1 (MQ=255)                                                                                                                                                                                                                                                 agcgatggtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTAc                                             <  1:280310‑M2/286‑1 (MQ=255)                                                                                                                                                                                                                                                        ttggtgcTGGAAGGATTCGAACCTTCGAATTCGATGACGGCAGATTTAGAGTCTACTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTTAGAAAAATGAACTGGTTGCGTAATCTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGtat                                      <  2:21182‑M2/293‑1 (MQ=255)                                                                                                                                                                                                                                                        gtggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGtat                                      <  1:1940‑M2/293‑1 (MQ=255)                                                                                                                                                                                                                                                          ggtgcGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAg                                    <  1:331774‑M2/295‑1 (MQ=255)                                                                                                                                                                                                                                                                     gATTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGCTTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTa                         >  1:125397/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      aTTCGAACCTTCGAAGTCGATGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTaa                        >  1:533689/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          tGACGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCAc    <  1:470673/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            aCGGCAGATTTAGAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGa  <  1:267314/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                 ACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCAGGGGTAATTCAAATTTTGAGGTAATGCTTGAGATGGTGGTGGGGGAAGGATTATTCGTCGCTTCGCTCCTCACCCTTCGGGCCGTTGCCTGTGGCAACGTTCTCTCGCTTTCGCTCGAATCGAACCTTAGTCGAAGGTTCTCACCCTTCCCCGATGAGTGCAAACTTTCACAATCTCACCGAAGTTACCACATCGCTGTGGTGAATTATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCCCACCACGGGGTAATGCTTTTTACTGGCCTGCTCCCTTATCGGGAAGCGGGGCGCATCATATCAAATGACGCGCCGCTGTAAAGTGTTAC‑GTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGA  >  NC_000913/1287293‑1287875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑