Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,969,375	C→A	intergenic (‑11/+8)	cheR ← / ← tap	chemotaxis protein methyltransferase/methyl‑accepting chemotaxis protein Tap

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,969,375	0	C	A	100.0%	213.6 / NA	58	intergenic (‑11/+8)	cheR/tap	chemotaxis protein methyltransferase/methyl‑accepting chemotaxis protein Tap
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/31);  total (27/31)

GATTCGTGGTCAGCGAATTGATAAACGCCTGCCACTCACCGCTGTGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGAT  >  NC_000913/1969088‑1969670                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                        gATTCGTGGTCAGCGAATTGATAAACGCCTGNCACTCACCGCTGTGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTNTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAAAAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAgg                                                                                                                                                                                                                                                                                             >  2:502622/1‑300 (MQ=255)   ttcttGGTCAGCGAATTGATAAACGCCTGCCACTCACCGCTGGGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCTCGTTTATGGTCATCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGGAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCATAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGAt                                                                                                                                                                                                                                                                                           <  2:137400/296‑1 (MQ=255)   ttCGTGGTCAGCGAATTGATAAACGCCTGCCACTCACCGCTGTGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGAt                                                                                                                                                                                                                                                                                           <  1:443867/300‑1 (MQ=255)   ttCGTGGTCAGCGAATGGAGAAACGCCTGCCACTCACCGCTGTGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGAt                                                                                                                                                                                                                                                                                           <  2:110984/300‑1 (MQ=255)                   tGATAAACGCCTGCCACTCATCACTGTGCTGATTAGATTCCAGCAATTTCAGATAAGGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCATTCGTTTGTAACCCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGACCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCGCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAAttt                                                                                                                                                                                                                                                                           <  2:42557/300‑1 (MQ=255)                                  gcTCACCGCTGTGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCGGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGTGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATCAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCCCTGGCGCAATTTGTAACTGCACCGACt                                                                                                                                                                                                                                                            >  2:450807/2‑300 (MQ=255)                                  aCTCACCGCTGTGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACt                                                                                                                                                                                                                                                            >  1:592368/1‑300 (MQ=255)                                   cTCACCGCTGTGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTc                                                                                                                                                                                                                                                           >  1:276679/1‑300 (MQ=255)                                       ccGCTGTGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGt                                                                                                                                                                                                                                                       <  1:421486/300‑1 (MQ=255)                                            gtgCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCAGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCACGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCGTCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATCGCGCCTTCTAAATCAATTAAGGATACCACTGGCGCAATTCGTAACTGCACCGACTCATGTCGTGc                                                                                                                                                                                                                                                  >  2:154614/1‑300 (MQ=255)                                                 gATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACtt                                                                                                                                                                                                                                             >  1:176937/1‑300 (MQ=255)                                                              cAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAGCGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTAGGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGGTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCGTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAg                                                                                                                                                                                                                                <  2:102319/300‑1 (MQ=255)                                                                       tagatgtgtataagagacaggTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATAc                                                                                                                                                                                                                       <  1:699396/280‑1 (MQ=255)                                                                                    tCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGGTGATAGATCAATTGACTTATCCGCCGNAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACNGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGcgac                                                                                                                                                                                                          <  2:571205/300‑1 (MQ=255)                                                                                           gTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATAcctgtctcttatacacatctc                                                                                                                                                                                                   >  2:685980/1‑281 (MQ=255)                                                                                                               cgaACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTNTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACNCGACGAAAGATGGTCGGCCTGATTCGCCAgt                                                                                                                                                                               <  2:493744/300‑1 (MQ=255)                                                                                                                 aaCCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAgttg                                                                                                                                                                             <  1:283807/300‑1 (MQ=255)                                                                                                                        cGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGtt                                                                                                                                                                      >  1:451003/1‑300 (MQ=255)                                                                                                                          gTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAANACGTTTGCCCACAGGGCAGAGATGAANTCATAGCGCCTTCTAAATCACTTCAGGATACCACGGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGc                                                                                                                                                                    >  2:475438/1‑300 (MQ=255)                                                                                                                          gTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGc                                                                                                                                                                    >  2:682506/1‑300 (MQ=255)                                                                                                                          gTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACCTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTAATTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGc                                                                                                                                                                    >  1:255144/1‑300 (MQ=255)                                                                                                                          gTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCAATTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGc                                                                                                                                                                    >  2:375355/1‑300 (MQ=255)                                                                                                                                     tGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAGAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGctt                                                                                                                                                         >  2:251430/1‑300 (MQ=255)                                                                                                                                        cgcttttATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGcttctt                                                                                                                                                      <  2:305434/296‑1 (MQ=255)                                                                                                                                            tttATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTAc                                                                                                                                                  >  2:242188/1‑300 (MQ=255)                                                                                                                                             ttaggggcAGCCAGAACGATCCCTGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCTTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGACCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATTTCGTGCTACTTCATGGTCTTCAAGGGGAAATACCGCCACACGCTACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTAcc                                                                                                                                                 <  2:683357/293‑1 (MQ=255)                                                                                                                                                          gAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTct                                                                                                                                    >  1:533279/1‑300 (MQ=255)                                                                                                                                                                 cccGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGt                                                                                                                             <  1:715961/300‑1 (MQ=255)                                                                                                                                                                   cGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCTAGGCGTTCTGCTGAGTCa                                                                                                                           <  1:149026/300‑1 (MQ=255)                                                                                                                                                                          ttGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATc                                                                                                                    <  2:154474/300‑1 (MQ=255)                                                                                                                                                                            gATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAAGAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGNAACTGCACCGACTCATGTCGTGCTACNTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCa                                                                                                                  <  2:480783/300‑1 (MQ=255)                                                                                                                                                                                gATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTg                                                                                                              >  1:83814/1‑300 (MQ=255)                                                                                                                                                                                          cTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGCCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTTAGTCACCTGATCCATCTGGCTGACAGcc                                                                                                    <  2:153221/300‑1 (MQ=255)                                                                                                                                                                                                   cgAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATANCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAAcc                                                                                           <  1:487481/300‑1 (MQ=255)                                                                                                                                                                                                    gAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCt                                                                                          <  1:70597/300‑1 (MQ=255)                                                                                                                                                                                                    gAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCGTCAAGGGTAAATACCGCCACACGCGACGAAAGATGTTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCt                                                                                          <  2:394251/300‑1 (MQ=255)                                                                                                                                                                                                        aTGCGCGTCGGAAAGCGCCATGCGCTCGGTCGTCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCATCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTc                                                                                      <  2:150872/300‑1 (MQ=255)                                                                                                                                                                                                             cgTCGGAAAGCGCCAGGCGCTCGTTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATcc                                                                                 <  1:611267/300‑1 (MQ=255)                                                                                                                                                                                                             cgTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATcc                                                                                 <  1:590381/300‑1 (MQ=255)                                                                                                                                                                                                               tCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGNCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATcccc                                                                               >  1:489686/1‑300 (MQ=255)                                                                                                                                                                                                                       cgcCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTNGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGNTTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTCATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCtgttg                                                                       >  2:566673/1‑300 (MQ=255)                                                                                                                                                                                                                             gcgcTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGttcttc                                                                 >  1:329316/1‑300 (MQ=255)                                                                                                                                                                                                                             gcgcTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGCGCAGAGATGAAGTNATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGCTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGttcttc                                                                 <  2:520307/300‑1 (MQ=255)                                                                                                                                                                                                                                    gTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCa                                                          >  1:352932/1‑300 (MQ=255)                                                                                                                                                                                                                                           gTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCaa                                                   <  2:200686/300‑1 (MQ=255)                                                                                                                                                                                                                                                          tttGCCCACAGGGCATAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCTACTCATTTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGt                                    <  1:186743/300‑1 (MQ=255)                                                                                                                                                                                                                                                          tttGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGt                                    >  1:121695/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  caGGGCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCAcg                            >  1:157189/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gggCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCAcgcg                          >  1:351834/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gggCAGAGATGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTNCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCANAGGCAATTTCTCCCATAATGTCGTTCAcgcg                          <  2:517714/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        atagaTGAAGTCATAGCGCCTTCTAAATTACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTc                      <  2:40286/298‑1 (MQ=255)                                                                                                                                                                                                                                                                           gaTGAAGTCATAGCGCCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCAcc                   >  1:206445/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        cgcCTTNTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTNATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAatat      <  2:582343/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        cgcCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAatat      <  2:235797/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         gcCTTCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATc     <  2:152761/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            ttCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCACTCCCCAGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGAt  >  2:427439/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            ttCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGAt  >  1:353372/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            ttCTAAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCATGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCACCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGAt  >  1:311514/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                        GATTCGTGGTCAGCGAATTGATAAACGCCTGCCACTCACCGCTGTGCTGATTAGATTCCAGCAAGTTCAGATAATGACCGAAATCCGTCAGTCCCAGCGAACGCAAACGACGAACCAGTCGGTTGTAAACCATGTCGCGTTTATGGTCAGCCAGAACGATCCCGGCTCGTTGATAGATCAATTGACTTATCCGCCGAAAATGCGCGTCGGAAAGCGCCAGGCGCTCGGTCATCTGTAACAATAAAGACGTTTGCCCACAGGGCAGAGATGAAGTCATAGCGCCTTCTCAATCACTTCAGGATACCACTGGCGCAATTTGTAACTGCACCGACTCATGTCGTGCTACTTCATGTTCTTCAAGGGTAAATACCGCCACACGCGACGAAAGATGGTCGGCCTGATTCGCCAGTTGTTCCGTTGCCACCGCCGCTTCTTCTACCAGCGAGGCGTTCTGCTGAGTCACCTGATCCATCTGGCTGACAGCCTGTGCAACCTGCTCAATCCCCCGCTGTTGTTCTTCCGACGCAGAGGCAATTTCTCCCATAATGTCGTTCACGCGGGTCACCGAACTGACAATATCGAT  >  NC_000913/1969088‑1969670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑