Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	984,313	C→T	intergenic (+16/+206)	gloC → / ← aspC	hydroxyacylglutathione hydrolase GloC/aspartate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	984,313	0	C	T	100.0%	195.0 / NA	54	intergenic (+16/+206)	gloC/aspC	hydroxyacylglutathione hydrolase GloC/aspartate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/24);  total (30/24)

CTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGAC  >  NC_000913/984017‑984592                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                        cTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGc                                                                                                                                                                                                                                                                                      >  1:351030/1‑300 (MQ=255)         gtgtTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTaagg                                                                                                                                                                                                                                                                              <  1:545272/300‑3 (MQ=255)         gtgtTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATg                                                                                                                                                                                                                                                                              >  1:666776/1‑300 (MQ=255)          tgtTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGc                                                                                                                                                                                                                                                                             <  1:435743/300‑1 (MQ=255)              aCATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTCATGAACGCCTGCATAATCCCTTCCTGCAAAACGAAATGCCCGTCTGGTAGGGCACATAAAAAAGCTCGCTTTTAATGCTgga                                                                                                                                                                                                                                                                         >  2:80284/1‑299 (MQ=255)                   gCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGa                                                                                                                                                                                                                                                                    >  2:427260/1‑300 (MQ=255)                   gCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGa                                                                                                                                                                                                                                                                    >  1:541030/1‑300 (MQ=255)                   gCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTACTTTCAAAGGCGGAATCGAGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCGAAGATAAATTACTGCAACTGGGGTATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTACTGCAAGACGAACCGCCAGTCTGGTAGGGCACCTAAAAAAGCTCGCTCTTAATGCTGGCATGGa                                                                                                                                                                                                                                                                    >  1:189839/1‑300 (MQ=255)                        gggCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCt                                                                                                                                                                                                                                                               >  1:230829/1‑300 (MQ=255)                         ggCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTg                                                                                                                                                                                                                                                              >  1:463157/1‑300 (MQ=255)                         ggCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGACTGGATTTCTg                                                                                                                                                                                                                                                              >  2:449257/1‑300 (MQ=255)                         ggCATACGCAGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTGTTCTTAAAGGCGGAGTAGGGCGCAGTGACCTCCAGAGTGGCGATCATAATCAACTGATNTCTTCAATCCAAGATAAATTGCTGCCNATGGGGGATGACGTGATATTTATTCCGGGTCGCGGAACATTATCCCCACTTGGTTATGAACACCTGCATGATTCATTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCAAATAAAAAAGCTCGCCCTCACTGCTGGCCTGGATTTccg                                                                                                                                                                                                                                                              >  2:487307/1‑298 (MQ=255)                                                ttttttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACTTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGcc                                                                                                                                                                                                                                       <  1:191374/300‑1 (MQ=255)                                                ttttgtGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGcc                                                                                                                                                                                                                                       <  2:444205/300‑1 (MQ=255)                                                                     ctgctgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCATTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTa                                                                                                                                                                                                                  >  2:217799/1‑300 (MQ=255)                                                                       gctgATTTCTGGCGATGTTATTTTCAAAGGCTGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATc                                                                                                                                                                                                                <  1:469432/300‑1 (MQ=255)                                                                       gctgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATc                                                                                                                                                                                                                <  1:104865/300‑1 (MQ=255)                                                                        ctgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATcc                                                                                                                                                                                                               <  2:319464/300‑1 (MQ=255)                                                                                                       gAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGTGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATAtt                                                                                                                                                                                >  2:212228/1‑300 (MQ=255)                                                                                                       gAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATAtt                                                                                                                                                                                >  1:63216/1‑300 (MQ=255)                                                                                                       gAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCACGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATAtt                                                                                                                                                                                >  2:684857/1‑300 (MQ=255)                                                                                                             ggCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAggg                                                                                                                                                                          <  1:320982/300‑1 (MQ=255)                                                                                                                       cTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAgg                                                                                                                                                                >  1:661811/1‑300 (MQ=255)                                                                                                                              cgTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGTTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATa                                                                                                                                                         <  2:83162/300‑1 (MQ=255)                                                                                                                              cgTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGNACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGaga                                                                                                                                                         <  1:507070/300‑3 (MQ=255)                                                                                                                              cgTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATa                                                                                                                                                         <  2:705359/300‑1 (MQ=255)                                                                                                                              cgTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATa                                                                                                                                                         <  1:297046/300‑1 (MQ=255)                                                                                                                                        aTAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATNCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTNGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAgcgc                                                                                                                                               <  2:564760/300‑1 (MQ=255)                                                                                                                                        aTAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAgcgc                                                                                                                                               <  1:632273/300‑1 (MQ=255)                                                                                                                                                                          gctgcCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGACTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGTGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGc                                                                                                             >  1:225760/1‑300 (MQ=255)                                                                                                                                                                               cACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCAGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGcaagct                                                                                                        >  1:708863/1‑295 (MQ=255)                                                                                                                                                                                 gggggggATGAAGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCATTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGTTGGCCTGGATTTCTGGCACAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTATAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCACTGTTGCATTTGTCATCAGTCTCAGCCCGCttt                                                                                                      >  2:277290/3‑300 (MQ=39)                                                                                                                                                                                                atatTTATGCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAAGCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCAtt                                                                                       <  2:229686/300‑1 (MQ=255)                                                                                                                                                                                                            ctgcgagatgtgtataagagacagggTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCt                                                                           >  1:119396/25‑300 (MQ=255)                                                                                                                                                                                                            ctgcgagatgtgtataagagacagggTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTAATTGTTTTTAATGCt                                                                           >  1:119426/25‑300 (MQ=38)                                                                                                                                                                                                             gTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCtt                                                                          >  2:275090/1‑300 (MQ=255)                                                                                                                                                                                                                  ggACCATTATCCACGCTTGGTTATGAACGCCTGCATAATCCCTTCCTTCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAGAAGCTCGCTTTTAATGCTGGTCGGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCGTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGc                                                                     <  2:354986/300‑1 (MQ=255)                                                                                                                                                                                                                    aCCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCAc                                                                   <  1:425672/300‑1 (MQ=255)                                                                                                                                                                                                                         tctCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCCAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCTGCTTTTCAGCGGGATTCATTGTTTTTAATGCTTACAGCACTGCca                                                              >  1:346460/3‑300 (MQ=255)                                                                                                                                                                                                                         tATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCca                                                              <  1:329350/300‑1 (MQ=255)                                                                                                                                                                                                                          aTCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCGGTCTAAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTACcac                                                             >  2:358885/1‑300 (MQ=255)                                                                                                                                                                                                                                             cGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGa                                          >  1:207991/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCAt                                     >  1:336442/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTAAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCAt                                     >  1:86160/1‑300 (MQ=255)                                                                                                                                                                                                                                                         ccattcttGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTTGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCCCAGCCCGCTTTTCAGCGGGCTCCATTGTTTTTAATGCTTACAGCACTGCCACAATCACTTCGCACAGCGGAGCCATGTTATCt                              <  2:335265/293‑1 (MQ=38)                                                                                                                                                                                                                                                           cTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTNTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTNATCTgg                            >  2:585412/1‑300 (MQ=255)                                                                                                                                                                                                                                                           cTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTGTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTgg                            <  2:648913/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aaGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTTGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATcc                    <  2:457470/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aaGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATcc                    <  1:268245/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                cGTCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTAGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTAc       >  2:394524/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  tCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTAcgc     <  1:456581/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tCTGGTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTAcgc     <  2:654297/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     ggTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCACGTTATCTGGTGTCATCCCGGCCACATTTACGCGaa  >  2:141658/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                     ggTAAGGCACATAAAAAAGCTCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCAATGTTATCTGGTGTCATCCCGGCCACATTTACGCGAc  >  1:676175/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                        CTTTACAGGTGTTACATTGCCCTGGGCATACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTGGCCTGGATTTCTGGCAAAGTGCGCTTTGTTTATGCCGGATGCGGCACGAGCGCCTTATCCGGCCTACAAAATCGTGCAAATTCAAAATATTGCAGGGGACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTCTCAGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACAGCACTGCCACAATCGCTTCGCACAGCGGAGCCATGTTATCTGGTGTCATCCCGGCCACATTTACGCGAC  >  NC_000913/984017‑984592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑