Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,935,118	C→T	L399L (CTG→CTA)	zwf ←	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,935,118	0	C	T	100.0%	191.5 / NA	52	L399L (CTG→CTA)	zwf	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/18);  total (34/18)

GCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTC  >  NC_000913/1934830‑1935405                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                gcgcAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCTCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGttt                                                                                                                                                                                                                                                                                      >  1:385618/1‑300 (MQ=255)            aCTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGc                                                                                                                                                                                                                                                                           >  1:620573/1‑300 (MQ=255)             cTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGcc                                                                                                                                                                                                                                                                          <  1:281579/300‑1 (MQ=255)                       ttCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTAAGTAATGGAGTCTCCCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAATAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGTTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAAGCACGGAACTTTa                                                                                                                                                                                                                                                                >  2:88962/1‑300 (MQ=255)                          cAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTc                                                                                                                                                                                                                                                             <  1:260421/300‑1 (MQ=255)                                 gACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCt                                                                                                                                                                                                                                                      >  1:561673/1‑300 (MQ=255)                                    cATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGa                                                                                                                                                                                                                                                   <  1:561647/300‑1 (MQ=255)                                    cATCACGAGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGGTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGAGTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGa                                                                                                                                                                                                                                                   <  1:135078/300‑1 (MQ=255)                                          gggTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATATGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCa                                                                                                                                                                                                                                             <  2:690144/300‑1 (MQ=255)                                          gggTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTNCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGANTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCa                                                                                                                                                                                                                                             <  2:667188/300‑1 (MQ=255)                                              aaTCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGcc                                                                                                                                                                                                                                         <  1:127467/300‑1 (MQ=255)                                               atcatcGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCt                                                                                                                                                                                                                                        >  2:179785/1‑300 (MQ=255)                                               atcatcGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCt                                                                                                                                                                                                                                        >  1:450930/1‑300 (MQ=255)                                                    cGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTtcatc                                                                                                                                                                                                                                   <  1:659806/300‑1 (MQ=255)                                                               cAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGAc                                                                                                                                                                                                                        <  1:210289/300‑1 (MQ=255)                                                                     gTCCCCAGTTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCATTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAg                                                                                                                                                                                                                  <  2:219821/300‑1 (MQ=255)                                                                                 cGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTAtttt                                                                                                                                                                                                      <  2:650583/300‑3 (MQ=255)                                                                                                 cGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCa                                                                                                                                                                                      <  1:445116/300‑1 (MQ=255)                                                                                                          gttGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCttt                                                                                                                                                                             >  2:707141/2‑300 (MQ=255)                                                                                                                              cAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGgtg                                                                                                                                                         >  1:383518/1‑300 (MQ=255)                                                                                                                                gTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCAAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGtt                                                                                                                                                       >  1:312914/1‑300 (MQ=255)                                                                                                                                gTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCATGAATACCACGCATGGTTTACAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGGTTTGTAGGTTATGTTTGTGGTCAAGGCAAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGtt                                                                                                                                                       >  2:80468/1‑300 (MQ=255)                                                                                                                                        gTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATa                                                                                                                                               >  1:618668/1‑300 (MQ=255)                                                                                                                                                         cttcttCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAAc                                                                                                                              >  1:67732/1‑300 (MQ=255)                                                                                                                                                                    tcgtcgCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAANAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGc                                                                                                                   >  2:533297/1‑300 (MQ=255)                                                                                                                                                                    tcgtcgCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGc                                                                                                                   >  1:545649/1‑300 (MQ=255)                                                                                                                                                                    tcgtcgCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGc                                                                                                                   >  2:195073/1‑300 (MQ=255)                                                                                                                                                                          cgACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCTCCAGATGTGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGATGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGt                                                                                                             <  2:86410/300‑1 (MQ=255)                                                                                                                                                                             cGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTa                                                                                                          >  2:651276/1‑300 (MQ=255)                                                                                                                                                                              gTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTAc                                                                                                         >  1:371342/1‑300 (MQ=255)                                                                                                                                                                              gTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTAc                                                                                                         >  2:288454/1‑300 (MQ=255)                                                                                                                                                                               tACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTAcc                                                                                                        >  1:349100/1‑300 (MQ=255)                                                                                                                                                                                          ccTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGCAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGt                                                                                             >  2:674278/1‑300 (MQ=255)                                                                                                                                                                                          ccTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGt                                                                                             >  1:263929/1‑300 (MQ=255)                                                                                                                                                                                          ccTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGt                                                                                             >  2:258839/1‑300 (MQ=255)                                                                                                                                                                                          ccTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTCTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGt                                                                                             >  1:256111/1‑300 (MQ=255)                                                                                                                                                                                             gAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAATATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGa                                                                                          >  2:241630/1‑300 (MQ=255)                                                                                                                                                                                             gAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCNGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAANAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGa                                                                                          >  2:667583/1‑300 (MQ=255)                                                                                                                                                                                                tACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATg                                                                                       <  1:257347/300‑1 (MQ=255)                                                                                                                                                                                                       cATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACAcc                                                                                <  1:180487/300‑1 (MQ=255)                                                                                                                                                                                                           gTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTNAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCacacaagcc                                                                            >  2:157503/1‑295 (MQ=255)                                                                                                                                                                                                           gTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAAATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGcc                                                                            <  2:225399/300‑1 (MQ=255)                                                                                                                                                                                                             ttCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCa                                                                          >  1:41962/1‑300 (MQ=255)                                                                                                                                                                                                                                        cGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGAt                                               >  1:96155/1‑300 (MQ=255)                                                                                                                                                                                                                                        cGCCAGACGCGTCTGATTAACGGTTTCTGAATAGCTCAGACCCAGCGTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAAGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGTAGAAGGATAGTCAGTTTGTTTTGAGGCAGATCCTGCCAAAATTCTTTACACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGAATTCAGAACATTTGGTCGGTAGACGTTTACCAGTACGCCGGTAGAATGGCATACCGGACCAGCGCAAGGTATAAATGTAGACGTGGAt                                               >  2:56264/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCGGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGtt              <  2:143984/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gCTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGtt              >  1:393043/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          cTCAGATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTNTTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCANTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTc             >  2:670388/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCgcgc         >  1:317565/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAATTTATTTTGCAGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTTgcgc         >  2:9576/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gATCCAGCTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGCTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACTGGCCCAGCGCCAGTTATCAATGTCGAAGCGGATCGCCACGAAAGTTTATGTATTGCTGCTCTTGTTCgcgc         >  2:245698/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     cTTGGTGATTTGTAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTTTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCtcttc  <  2:552191/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                GCGCAGATATTACTCAAACTCATTCCAGGAACGACCATCACGGGTAATCATCGCCACCGAGGCAACGGGTCCCCAGGTTCCGGCCTGATACGGTTTCGGCGCATCATTGTCCATCGCCCACGCCTCAGTAATGGAGTCTACCCATTTCCAGGCTTCTTCCACTTCGTCGCGACGTACAAACAGTGCCTGAATACCACGCATGGTTTCCAGCAGCAAACGTTCATAGGCATCCGCCAGATGCGTCTGATTAAAGGTTTCTGAATAGCTCAGATCCAGCTTGGTGATTTGCAGGTTATGTTTGTGGTCAAGGCCAGGAACTTTATTCAGTACCTGGATATCCACGCCTTCATCAGGTTGCAGACGGATAGTCAGTTTATTCTGCGGCAGATCCTGCCACGATTCTTTAAACAGATTCAGTTCAGGTGTTTTGAAATAGACCACGACTTCAGAACATTTGGTCGGCAGACGTTTACCAGTACGCAGGTAGAATGGCACACCGGCCCAGCGCCAGTTATCAATGTCGACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTC  >  NC_000913/1934830‑1935405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑