Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,072,103	A→G	V19V (GTT→GTC)	rutC ←	3‑aminoacrylate deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,072,103	0	A	G	100.0%	158.9 / NA	42	V19V (GTT→GTC)	rutC	3‑aminoacrylate deaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/17);  total (25/17)

GCGTCAGGTTTTACCAGTCCGCACTGAATGCAGAATCGCGCCGGTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGT  >  NC_000913/1071813‑1072377                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                   gcgTCAGGTTTTACCAGTCCGCACTGAATGCAGAATCGCGCCGGTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACAACACGCCATCAGCCAGCGTGCCGGGGACGAAgggg                                                                                                                                                                                                                                                                           >  2:43591/1‑300 (MQ=255)      aGGTTTTACCAGTCCGCACTGAATGCAGAATCGCGCCGGTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTACAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGANAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGATCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAg                                                                                                                                                                                                                                                                      >  2:166266/1‑300 (MQ=255)       ggTTTTACCAGTCCGCACTGAATGCAGAATCGCGCCGGTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCAGGGGACGAAGGGGGCCAGc                                                                                                                                                                                                                                                                     >  2:327663/1‑300 (MQ=255)                aGTCCGCACTGAATGCAGAATCGCGCCGGTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCgctg                                                                                                                                                                                                                                                            >  1:348635/1‑300 (MQ=255)                 gTCCGCACTGAATGCAGAATCGCGCCGGTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATAAGCCAGCGTGCCGGGGACGAAGGGGGCAGGCGATGCgctgc                                                                                                                                                                                                                                                           >  2:100952/1‑300 (MQ=255)                     gCACTGAATGCAGAATCGCGCCGGTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGcc                                                                                                                                                                                                                                                       >  1:112062/1‑300 (MQ=255)                        cTGAATGCAGAATCGCGCCGTTTTATCACCCTGAAAAAACTCGTCTTAAATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGNCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCNCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGc                                                                                                                                                                                                                                                    <  2:562599/300‑1 (MQ=255)                          gAATGCAGAATCGCGCCGGTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCgg                                                                                                                                                                                                                                                  >  1:291914/1‑300 (MQ=255)                                           ggTTTATCACCCGGAAAAAACTCGGCGTAGANTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTNGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGAtttt                                                                                                                                                                                                                                 >  2:512454/1‑300 (MQ=255)                                            gTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGACAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTg                                                                                                                                                                                                                                >  2:702186/1‑300 (MQ=255)                                                        gAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAAc                                                                                                                                                                                                                    <  1:129965/300‑1 (MQ=255)                                                          aaaaaCTTGGCGTAGATTTCGTTAACCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCGGCGTGCCACCCTCCGTCTCGATCACCTGGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTCGCATCGTTAAACTc                                                                                                                                                                                                                  <  2:113994/300‑1 (MQ=255)                                                                  gtcGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGNGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCg                                                                                                                                                                                                          <  2:155777/298‑1 (MQ=255)                                                                  ggCGTAGATTTCGTTAATCGCTGCGTAATTTTTCCATTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGTGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCg                                                                                                                                                                                                          <  1:143356/300‑1 (MQ=255)                                                                  ggCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCg                                                                                                                                                                                                          <  1:174381/300‑1 (MQ=255)                                                                                                    cAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGACCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGc                                                                                                                                                                        <  2:317617/300‑1 (MQ=255)                                                                                                          gTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGANGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATg                                                                                                                                                                  >  1:485830/1‑300 (MQ=255)                                                                                                                           gAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGTAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGAcc                                                                                                                                                 <  1:110943/300‑1 (MQ=255)                                                                                                                           gAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGAcc                                                                                                                                                 <  1:702825/300‑1 (MQ=255)                                                                                                                                   caTCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCaaa                                                                                                                                         >  1:36527/1‑300 (MQ=255)                                                                                                                                         ccATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGt                                                                                                                                   <  1:613968/300‑1 (MQ=255)                                                                                                                                           aTCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGttt                                                                                                                                 >  2:639738/1‑300 (MQ=255)                                                                                                                                                      cgccgTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGaac                                                                                                                      <  1:176624/300‑1 (MQ=255)                                                                                                                                                            ctcGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAAcgcg                                                                                                                >  1:717297/1‑300 (MQ=255)                                                                                                                                                              cGATTACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGCCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGc                                                                                                              >  2:46311/1‑300 (MQ=255)                                                                                                                                                              cGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTAGATATTGAACAACGCGGc                                                                                                              >  1:646161/1‑300 (MQ=255)                                                                                                                                                                       tGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTgcgc                                                                                                     <  1:195650/300‑1 (MQ=255)                                                                                                                                                                                            tgCGGGTTTGCGCCTTTGGTTCATCGGCAAACAGCACGTTATTATGTTGATCAANAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAANAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTg                                                                                <  2:661995/299‑1 (MQ=255)                                                                                                                                                                                               gggTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATGATGGTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTGAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAACTTCGGCCCCGCCTGGTGAGt                                                                             <  2:70987/300‑1 (MQ=255)                                                                                                                                                                                                              gttCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGcac                                                              <  1:80038/298‑1 (MQ=255)                                                                                                                                                                                                                   tCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGc                                                         >  1:260822/1‑300 (MQ=255)                                                                                                                                                                                                                                  ttattaTGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAACGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGaa                                          >  2:91648/1‑300 (MQ=255)                                                                                                                                                                                                                                  ttattaTGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGaa                                          >  2:394920/1‑300 (MQ=255)                                                                                                                                                                                                                                  ttattaTGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCAGAAATACTCCAGaa                                          >  1:76978/1‑300 (MQ=255)                                                                                                                                                                                                                                  ttattaTGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCAGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGaa                                          >  1:268490/1‑300 (MQ=255)                                                                                                                                                                                                                                  ttattaTGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGAAGAATGTTTCGACGTCGCTGACCCAGCCAAACAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGaa                                          >  2:118048/1‑300 (MQ=255)                                                                                                                                                                                                                                                        gCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGa                    >  2:617576/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGAtt                  >  1:662774/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gccATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGANCAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAg          >  1:527976/3‑300 (MQ=255)                                                                                                                                                                                                                                                                  gaCATACACCACGCCATCAGCCAGCGTGCCGGGGACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAg          >  1:322702/1‑300 (MQ=255)                                                                                                                                                                                                                                                                     aTACACCACGCCATCAACCAGTGTGCCGGGGACGAAGGGGGCCAGCGGTACGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACg       <  2:207907/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          ccacGCTATCAGACAGCGTGCCGGGGGCTAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGGTAAACTCCTTAAGCGATATGAGCAGAGGACGTCGGAGAAAGCGCGTCGCAGAAGGTTGCGACGGCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTTGCGTAGCGTCGATTCGACGCAGACGTGGGt  <  2:239033/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                   GCGTCAGGTTTTACCAGTCCGCACTGAATGCAGAATCGCGCCGGTTTATCACCCGGAAAAAACTCGGCGTAGATTTCGTTAATCGCGGCGTAATTTTTCCAGTCGGTAATAAAGATGCTGTTGAAGGTCACATCCGCCATCGTGCCACCCGCCGTCTCGATCACCTTGCGGATAGTTTCCAGAACGTGGCGGGTTTGCGCCTTTGGGTCATCGGCAAACAGCACGTTATTATGTTGATCAAAAGCCAGCGTACCGGAGACATACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCGTCTTCAAGCACCACGCCGAAATACTCCAGAAAAAAGCCGTCGCGTAGCGTCGATTCGACGCAGACGTTGGT  >  NC_000913/1071813‑1072377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑