breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,645 G→A W282* (TGG→TAG)  thrB → homoserine kinase
RA 71,170 T→C S262P (TCG→CCG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 80,168 C→T V233I (GTT→ATT)  leuC ← 3‑isopropylmalate dehydratase subunit LeuC
RA 81,102 G→A S286L (TCG→TTG)  leuB ← 3‑isopropylmalate dehydrogenase
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
MC JC 255,892 Δ18,063 bp IS5‑mediated gpt[ykfC] 26 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 292,019 C→T L174L (CTA→TTA)  yagJ → protein YagJ
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
RA 321,260 A→T intergenic (+179/‑348) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
RA 335,909 A→G intergenic (+24/‑16) yahD → / → yahE ankyrin repeat‑containing protein YahD/DUF2877 domain‑containing protein YahE
RA 410,666 C→T P175S (CCT→TCT)  mak → fructokinase
RA 421,027 C→T R14R (CGC→CGT proY → putative transporter ProY
RA 428,720 G→A K358K (AAG→AAA secD → Sec translocon accessory complex subunit SecD
RA 431,912 C→T W34* (TGG→TGA tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 465,005 G→A V181V (GTC→GTT queC ← 7‑cyano‑7‑deazaguanine synthase
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
RA 482,771 A→T Y545N (TAT→AAT)  acrB ← multidrug efflux pump RND permease AcrB
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 534,742 G→A G276D (GGT→GAT)  gcl → glyoxylate carboligase
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 640,133 C→T L127L (CTC→CTT ahpF → alkyl hydroperoxide reductase, AhpF component
RA 694,941 G→A L5L (CTC→CTT miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 708,774 G→A A147A (GCG→GCA chiP → chitooligosaccharide outer membrane channel
RA 721,604 G→A R44C (CGT→TGT)  kdpE ← DNA‑binding transcriptional activator KdpE
RA 780,624 C→T noncoding (71/76 nt) lysT → tRNA‑Lys
RA 789,580 C→A E134* (GAA→TAA)  galK ← galactokinase
JC JC 820,914 IS5 (+) +4 bp coding (122‑125/714 nt) ybhM → Bax1‑I family protein YbhM
RA 822,527 C→T E405K (GAA→AAA)  clsB ← cardiolipin synthase B
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 908,169 C→T R38R (AGG→AGA ybjT ← putative NAD(P)‑binding protein YbjT
JC 924,170 Δ10 bp coding (907‑916/2277 nt) clpA → ATP‑dependent Clp protease ATP‑binding subunit ClpA
RA 1,038,477 2 bp→AA coding (738‑739/1545 nt) appC → cytochrome bd‑II subunit 1
RA 1,038,933 C→T S398S (TCC→TCT appC → cytochrome bd‑II subunit 1
JC JC 1,105,699 IS1 (+) +9 bp coding (286‑294/312 nt) ymdA → uncharacterized protein YmdA
RA 1,123,823 2 bp→AG coding (231‑232/648 nt) grxB ← reduced glutaredoxin 2
RA 1,147,792 T→A S58T (TCG→ACG)  plsX → putative phosphate acyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,201,548 A→C T18P (ACC→CCA)  ymfI → uncharacterized protein YmfI
RA 1,201,550 C→A T18P (ACC→CCA ymfI → uncharacterized protein YmfI
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
RA 1,261,462 G→A F138F (TTC→TTT prs ← ribose‑phosphate diphosphokinase
RA 1,265,412 T→A M134K (ATG→AAG)  prfA → peptide chain release factor RF1
RA 1,278,858 G→A G301D (GGT→GAT)  narK → nitrate:nitrite antiporter NarK
RA 1,279,307 G→A V451M (GTG→ATG)  narK → nitrate:nitrite antiporter NarK
RA 1,285,662 C→T A175V (GCG→GTG)  narJ → nitrate reductase 1 molybdenum cofactor assembly chaperone
RA 1,289,871 G→A W209* (TGG→TGA rssA → putative patatin‑like phospholipase RssA
JC JC 1,290,542 IS1 (+) +9 bp coding (301‑309/1014 nt) rssB → regulator of RpoS
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,308,684 T→C intergenic (‑39/‑104) clsA ← / → yciY cardiolipin synthase A/uncharacterized protein YciY
RA 1,331,059 2 bp→AT coding (12‑13/2598 nt) topA → DNA topoisomerase 1
RA 1,334,416 G→A E188K (GAA→AAA)  cysB → DNA‑binding transcriptional dual regulator CysB
RA 1,335,273 G→A W42* (TGG→TGA ymiA → uncharacterized protein YmiA
RA 1,336,316 G→A W162* (TGG→TGA acnA → aconitate hydratase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,379,534 G→A A551T (GCG→ACG)  ycjT → kojibiose phosphorylase
RA 1,380,878 G→A intergenic (+71/‑1069) ycjU → / → ompG beta‑phosphoglucomutase/outer membrane porin G
RA 1,381,391 G→A intergenic (+584/‑556) ycjU → / → ompG beta‑phosphoglucomutase/outer membrane porin G
RA 1,381,989 G→A G15R (GGA→AGA)  ompG → outer membrane porin G
RA 1,384,299 G→A E61E (GAG→GAA ycjX → DUF463 domain‑containing protein YcjX
RA 1,386,294 G→A V262I (GTA→ATA)  ycjF → DUF697 domain‑containing inner membrane protein YcjF
RA 1,389,732 G→A G268D (GGT→GAT)  ycjG → L‑Ala‑D/L‑Glu epimerase
RA 1,395,367 G→A R186C (CGT→TGT)  ynaI ← small conductance mechanosensitive channel YnaI
MC JC 1,397,239 Δ49,912 bp IS5‑mediated ynaJ[feaR] 61 genes
RA 1,447,847 G→A G110D (GGC→GAC)  feaB → phenylacetaldehyde dehydrogenase
RA 1,451,495 G→A intergenic (‑146/+102) tynA ← / ← paaZ copper‑containing amine oxidase/fused 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase and oxepin‑CoA hydrolase
RA 1,451,818 G→A L609L (CTG→TTG)  paaZ ← fused 3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase and oxepin‑CoA hydrolase
RA 1,471,130 G→A pseudogene (612/3495 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,476,960 G→A S606N (AGT→AAT)  ydbD → DUF2773 domain‑containing protein YdbD
RA 1,480,239 G→A G368R (GGG→AGG)  ynbC → hydrolase/methyltransferase domain‑containing protein YnbC
RA 1,481,364 G→A R152R (AGG→AGA ynbD → phosphatase domain‑containing protein YnbD
RA 1,485,508 G→A W816* (TGG→TGA hrpA → ATP‑dependent 3'‑>5' RNA helicase HrpA
RA 1,489,607 G→A G459D (GGC→GAC)  aldA → aldehyde dehydrogenase A
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,497,862 G→A G336E (GGG→GAG)  opgD → glucans biosynthesis protein D
RA 1,499,082 G→A E49K (GAG→AAG)  rimL → ribosomal‑protein‑L12‑serine N‑acetyltransferase
RA 1,502,803 G→A G116E (GGG→GAG)  ydcL → DUF3313 domain‑containing lipoprotein YdcL
RA 1,507,136 G→A C119Y (TGC→TAC)  rlhA → 23S rRNA 5‑hydroxycytidine C2501 synthase
RA 1,507,509 G→A G243G (GGG→GGA rlhA → 23S rRNA 5‑hydroxycytidine C2501 synthase
RA 1,510,901 G→A R300H (CGC→CAC)  ydcR → fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
RA 1,517,587 2 bp→TT intergenic (+25/‑60) ortT → / → ydcY orphan toxin OrtT/DUF2526 domain‑containing protein YdcY
RA 1,522,583 C→T Q161Q (CAG→CAA yncD ← putative TonB‑dependent outer membrane receptor
RA 1,541,037 A→G I518I (ATT→ATC narZ ← nitrate reductase Z subunit alpha
RA 1,541,058 G→A T511T (ACC→ACT narZ ← nitrate reductase Z subunit alpha
RA 1,548,796 G→A G466S (GGT→AGT)  fdnG → formate dehydrogenase N subunit alpha
RA 1,591,711 C→A E156* (GAG→TAG)  hipA ← serine/threonine‑protein kinase toxin HipA
RA 1,614,927 G→A E42K (GAA→AAA)  yneJ → putative DNA‑binding transcriptional regulator YneJ
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
RA 1,636,854 G→A G33G (GGG→GGA ynfO → DUF3950 domain‑containing protein YnfO
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,646,587 G→A Q61Q (CAG→CAA flxA → PF14282 family protein FlxA
RA 1,650,234 G→A intergenic (+249/+2494) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,660,102 C→T N678N (AAC→AAT ynfE → putative selenate reductase YnfE
RA 1,664,527 G→A D8N (GAT→AAT)  dmsD → redox enzyme maturation protein DmsD
RA 1,664,866 C→T Q121* (CAA→TAA)  dmsD → redox enzyme maturation protein DmsD
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,677,597 G→A Q103* (CAA→TAA)  pntA ← pyridine nucleotide transhydrogenase subunit alpha
RA 1,684,969 G→A Q237Q (CAG→CAA tus → DNA replication terminus site‑binding protein
RA 1,692,637 G→A S72F (TCC→TTC)  uidC ← outer membrane porin family protein UidC
RA 1,701,498 G→A C181Y (TGC→TAC)  malY → negative regulator of MalT activity/cystathionine beta‑lyase
RA 1,712,661 A→T intergenic (+503/‑108) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,719,133 G→A A157V (GCT→GTT)  anmK ← anhydro‑N‑acetylmuramic acid kinase
RA 1,723,658 G→A G513E (GGA→GAA)  ydhK → putative transporter YdhK
RA 1,730,562 G→A W492* (TGG→TGA lhr → ATP‑dependent helicase Lhr
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,751,720 C→T G2D (GGT→GAT)  ydhW ← uncharacterized protein YdhW
RA 1,766,605 G→A S694F (TCT→TTT)  ydiJ ← putative FAD‑linked oxidoreductase YdiJ
RA 1,766,814 G→A R624R (CGC→CGT ydiJ ← putative FAD‑linked oxidoreductase YdiJ
RA 1,780,275 G→A G220D (GGC→GAC)  ydiQ → putative electron transfer flavoprotein subunit YdiQ
RA 1,795,734 G→A G737G (GGC→GGT pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,795,878 G→A L689L (CTC→CTT pheT ← phenylalanine‑‑tRNA ligase subunit beta
RA 1,798,325 G→A F206F (TTC→TTT pheS ← phenylalanine‑‑tRNA ligase subunit alpha
RA 1,800,306 G→A L111L (CTC→CTT infC ← translation initiation factor IF‑3
RA 1,806,142 G→A intergenic (‑59/‑228) ydiY ← / → pfkB acid‑inducible putative outer membrane protein YdiY/6‑phosphofructokinase 2
RA 1,816,482 G→A F218F (TTC→TTT chbG ← chitooligosaccharide monodeacetylase ChbG
RA 1,826,466 G→A S153F (TCC→TTC)  astE ← succinylglutamate desuccinylase
RA 1,826,583 G→A A114V (GCT→GTT)  astE ← succinylglutamate desuccinylase
RA 1,829,039 G→A L232L (CTC→CTT astD ← aldehyde dehydrogenase
RA 1,829,270 G→A I155I (ATC→ATT astD ← aldehyde dehydrogenase
RA 1,833,008 G→A G194E (GGG→GAG)  xthA → exodeoxyribonuclease III
RA 1,834,594 G→A G160D (GGC→GAC)  ydjY → 4Fe‑4S ferredoxin‑type domain‑containing protein YdjY
RA 1,836,397 G→A E109K (GAG→AAG)  ynjB → putative ABC transporter periplasmic binding protein YnjB
RA 1,838,612 G→A V467V (GTG→GTA ynjC → putative ABC transporter membrane subunit YnjC
RA 1,839,359 G→A D205N (GAT→AAT)  ynjD → putative ABC transporter ATP‑binding protein YnjD
RA 1,841,392 G→A R4R (CGC→CGT ynjF ← CDP‑alcohol phosphatidyltransferase domain‑containing protein YnjF
RA 1,842,759 G→A G130D (GGC→GAC)  gdhA → glutamate dehydrogenase
RA 1,846,393 G→A Q190* (CAG→TAG)  topB ← DNA topoisomerase III
RA 1,847,078 G→A P311S (CCG→TCG)  selD ← selenide, water dikinase
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
RA 1,853,430 T→C I184V (ATT→GTT)  ydjE ← putative transporter YdjE
RA 1,854,611 G→A L82F (CTT→TTT)  ydjF ← putative DNA‑binding transcriptional regulator YdjF
RA 1,888,066 C→T A561T (GCC→ACC)  fadD ← long‑chain‑fatty‑acid‑‑CoA ligase
RA 1,888,507 C→T V414M (GTG→ATG)  fadD ← long‑chain‑fatty‑acid‑‑CoA ligase
RA 1,888,749 C→T G333D (GGT→GAT)  fadD ← long‑chain‑fatty‑acid‑‑CoA ligase
RA 1,889,965 C→T V190I (GTT→ATT)  yeaY ← Slp family lipoprotein YeaY
RA 1,890,357 C→T G59D (GGT→GAT)  yeaY ← Slp family lipoprotein YeaY
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,900,758 G→A I276I (ATC→ATT yoaE ← TerC family inner membrane protein YoaE
RA 1,901,699 C→T R11R (AGG→AGA yoaL ← protein YoaL
RA 1,905,730 A→G M15V (ATG→GTG)  mntP → Mn(2(+)) exporter
RA 2,023,559 A→G T223A (ACT→GCT)  fliR → flagellar biosynthesis protein FliR
RA 2,027,332 T→A E229V (GAA→GTA)  dgcQ ← diguanylate cyclase DgcQ
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
RA 2,090,780 C→T Q197* (CAA→TAA)  hisG → ATP phosphoribosyltransferase
RA 2,104,024 G→T I162I (ATC→ATA wbbK ← putative glycosyltransferase WbbK
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,120,969 G→A L196L (CTG→TTG)  wcaJ ← UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase
RA 2,142,598 G→A L117L (CTC→CTT udk ← uridine/cytidine kinase
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
JC JC 2,239,316 IS5 (–) +4 bp intergenic (‑27/+31) mglA ← / ← mglB D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit/D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,242,247 C→T G241R (GGG→AGG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter
RA 2,361,210 C→G E69Q (GAA→CAA)  rhmD ← L‑rhamnonate dehydratase
RA 2,404,402 G→A S71L (TCG→TTG)  nuoB ← NADH:quinone oxidoreductase subunit B
RA 2,481,608 A→G L190S (TTA→TCA)  emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,498,392 A→C intergenic (‑97/‑279) alaC ← / → pyrS glutamate‑‑pyruvate aminotransferase AlaC/sensor histidine kinase PyrS
RA 2,546,804 T→A D44E (GAT→GAA murP → N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,577,937 T→C E147E (GAA→GAG maeB ← malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,745,600 A→G Y107H (TAC→CAC)  rimM ← ribosome maturation factor RimM
RA 2,774,947 G→A G544S (GGT→AGT)  yfjW → uncharacterized protein YfjW
RA 2,867,358 +AA coding (194/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,880,635 T→C L138L (TTA→TTG casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,962,178 C→T S84N (AGC→AAC)  recC ← exodeoxyribonuclease V subunit RecC
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
MC JC 3,131,341 Δ128 bp IS5‑mediated yghQ ← putative transport protein YghQ
RA 3,169,395 C→T W94* (TGG→TGA ygiW ← BOF family protein YgiW
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,256,301 G→A S117S (TCC→TCT cyuA ← putative L‑cysteine desulfidase CyuA
RA 3,264,090 T→C T310A (ACG→GCG)  tdcC ← threonine/serine:H(+) symporter
RA 3,279,276 C→G F121L (TTC→TTG kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,344,624 C→T D227D (GAC→GAT lptB → lipopolysaccharide transport system ATP binding protein LptB
RA 3,354,130 C→T intergenic (‑80/‑595) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,355,840 C→T A372A (GCC→GCT gltB → glutamate synthase subunit GltB
RA 3,378,933 G→A C355C (TGC→TGT zapE ← cell division protein ZapE
RA 3,380,224 G→A V12I (GTC→ATC)  zapG → Z‑ring associated protein G
RA 3,383,255 G→A P352P (CCG→CCA degS → serine endoprotease
RA 3,387,090 G→A L367L (CTG→TTG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,387,098 G→A S364F (TCC→TTC)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,390,921 G→A P370S (CCG→TCG)  tldD ← metalloprotease subunit TldD
RA 3,391,506 G→A L175L (CTA→TTA)  tldD ← metalloprotease subunit TldD
RA 3,392,062 G→A intergenic (‑34/+396) tldD ← / ← yhdP metalloprotease subunit TldD/phospholipid transport factor YhdP
RA 3,397,465 G→A P111S (CCG→TCG)  rng ← RNase G
RA 3,398,163 G→A I72I (ATC→ATT yhdE ← nucleoside triphosphate pyrophosphatase YhdE
RA 3,399,245 G→A T245I (ACC→ATC)  mreC ← cell shape determining protein MreC
RA 3,402,640 G→A G231G (GGC→GGT csrD ← regulator of CsrB and CsrC decay
RA 3,411,222 G→A A315T (GCA→ACA)  dusB → tRNA‑dihydrouridine synthase B
RA 3,411,333 G→A Q21Q (CAG→CAA fis → DNA‑binding transcriptional dual regulator Fis
RA 3,411,689 G→T E13* (GAA→TAA)  yhdJ → DNA adenine methyltransferase
RA 3,412,692 G→A Q24Q (CAG→CAA yhdU → DUF2556 domain‑containing protein YhdU
RA 3,417,464 G→A G811D (GGC→GAC)  acrF → multidrug efflux pump RND permease AcrF
RA 3,423,433 G→A noncoding (110/120 nt) rrfF ← 5S ribosomal RNA
RA 3,430,178 G→A P221S (CCT→TCT)  aroE ← shikimate dehydrogenase
RA 3,433,348 G→A N71N (AAC→AAT smf ← protein Smf
RA 3,433,352 G→A P70L (CCC→CTC)  smf ← protein Smf
RA 3,433,900 C→T L71L (CTA→TTA)  def → peptide deformylase
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,486,175 G→A C19Y (TGC→TAC)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,499,989 G→A G27D (GGT→GAT)  frlA → fructoselysine/psicoselysine transporter
RA 3,500,075 G→A V56M (GTG→ATG)  frlA → fructoselysine/psicoselysine transporter
RA 3,506,695 C→T G214G (GGG→GGA yhfT ← uncharacterized protein YhfT
RA 3,507,249 C→T D30N (GAC→AAC)  yhfT ← uncharacterized protein YhfT
RA 3,511,196 G→A S76F (TCC→TTC)  yhfY ← PRD domain‑containing protein YhfY
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,523,972 G→A D368N (GAC→AAC)  mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,524,618 G→A G583D (GGC→GAC)  mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,529,763 G→A intergenic (+14/‑11) hslR → / → hslO heat shock protein Hsp15/molecular chaperone Hsp33
RA 3,530,911 T→C D510G (GAT→GGT)  yhgE ← putative transport protein YhgE
RA 3,532,269 G→A S57S (TCC→TCT yhgE ← putative transport protein YhgE
RA 3,553,283 G→A G67S (GGT→AGT)  malT → DNA‑binding transcriptional activator MalT
RA 3,553,965 G→A G294E (GGG→GAG)  malT → DNA‑binding transcriptional activator MalT
RA 3,555,047 2 bp→TG coding (1963‑1964/2706 nt) malT → DNA‑binding transcriptional activator MalT
RA 3,560,362 G→A intergenic (+496/+260) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,578,486 C→T V81I (GTA→ATA)  gntR ← DNA‑binding transcriptional repressor GntR
RA 3,589,062 C→T G40D (GGC→GAC)  ugpC ← sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
RA 3,591,926 C→T G134S (GGC→AGC)  ugpB ← sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein
RA 3,615,376 C→T P46S (CCG→TCG)  nikB → Ni(2(+)) ABC transporter membrane subunit NikB
RA 3,619,344 C→T P51P (CCC→CCT rhsB → rhs element protein RhsB
RA 3,646,970 G→T Q224H (CAG→CAT gor → glutathione reductase (NADPH)
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,649,847 C→T L305F (CTT→TTT)  arsB → arsenite/antimonite:H(+) antiporter
RA 3,665,264 C→T E183K (GAG→AAG)  gadX ← DNA‑binding transcriptional dual regulator GadX
RA 3,665,323 C→T R163Q (CGA→CAA)  gadX ← DNA‑binding transcriptional dual regulator GadX
RA 3,679,581 G→A G55R (GGA→AGA)  kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,696,355 C→T intergenic (‑170/‑103) yhjR ← / → bcsE PF10945 family protein YhjR/c‑di‑GMP‑binding protein BcsE
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,719,429 G→A intergenic (‑385/‑49) yiaF ← / → yiaG DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ
RA 3,730,560 C→T W69* (TGG→TAG)  xylA ← xylose isomerase
RA 3,744,021 C→T S93S (AGC→AGT yiaL → DUF386 domain‑containing protein YiaL
RA 3,748,394 C→T A437A (GCC→GCT lyxK → L‑xylulose kinase
RA 3,751,455 C→T V138V (GTG→GTA yiaT ← outer membrane protein YiaT
RA 3,754,203 C→T G76R (GGA→AGA)  yiaW ← DUF3302 domain‑containing protein YiaW
RA 3,758,605 C→T W419* (TGG→TGA selB ← selenocysteyl‑tRNA‑specific translation elongation factor
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,799,080 C→T R78K (AGA→AAA)  waaU ← putative ADP‑heptose:LPS heptosyltransferase 4
RA 3,806,032 C→T E346K (GAG→AAG)  waaG ← lipopolysaccharide glucosyltransferase I
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,817,167 C→T T164T (ACC→ACT yicC → putative RNase adaptor protein YicC
RA 3,838,839 C→T L198L (CTG→TTG)  yicL → putative transporter YicL
RA 3,858,148 C→T R86H (CGC→CAC)  yidJ ← putative sulfatase/phosphatase YidJ
RA 3,858,455 C→T R554R (CGG→CGA yidK ← putative transporter YidK
RA 3,916,243 A→T L378Q (CTG→CAG)  atpD ← ATP synthase F1 complex subunit beta
RA 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,931,779 T→C F155S (TTT→TCT)  kup → K(+):H(+) symporter Kup
RA 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho
RA 4,120,093 T→C Q86R (CAA→CGA)  menA ← 1,4‑dihydroxy‑2‑naphthoate octaprenyltransferase
RA 4,153,836 G→A Q338* (CAA→TAA)  argE ← acetylornithine deacetylase
MC JC 4,180,661 Δ6 bp coding (102‑107/366 nt) rplL → 50S ribosomal subunit protein L12
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,215,670 G→T D65Y (GAT→TAT)  aceB → malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,310,977 C→T A2T (GCC→ACC)  alsA ← D‑allose ABC transporter ATP binding subunit
RA 4,319,018 C→T D197N (GAC→AAC)  phnJ ← carbon‑phosphorus lyase core complex subunit PhnJ
RA 4,325,177 C→T intergenic (‑12/+121) phnC ← / ← yjdN phosphonate/phosphate ABC transporter ATP binding subunit/PF06983 family protein YjdN
RA 4,325,485 C→T G86D (GGC→GAC)  yjdN ← PF06983 family protein YjdN
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,332,923 C→T S117N (AGC→AAC)  basS ← sensor histidine kinase BasS
RA 4,338,692 C→T P610P (CCG→CCA adiA ← arginine decarboxylase, degradative
RA 4,340,671 C→T intergenic (‑150/+49) adiA ← / ← melR arginine decarboxylase, degradative/DNA‑binding transcriptional dual regulator MelR
RA 4,342,119 C→T T70I (ACC→ATC)  melA → alpha‑galactosidase
RA 4,347,486 C→T S420N (AGC→AAC)  dcuB ← anaerobic C4‑dicarboxylate transporter DcuB
RA 4,352,881 C→T intergenic (+1/‑27) ghoS → / → ghoT antitoxin of the GhoTS toxin‑antitoxin system/toxin of the GhoTS toxin‑antitoxin system
RA 4,364,530 C→T L171L (TTG→TTA dsbD ← thiol‑disulfide exchange protein DsbD
RA 4,369,788 C→T E209K (GAA→AAA)  yjeH ← L‑methionine/branched chain amino acid exporter
RA 4,384,749 C→T A304V (GCG→GTG)  yjeM → putative transporter YjeM
RA 4,386,553 C→T V940M (GTG→ATG)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,386,622 C→T E917K (GAA→AAA)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,386,718 C→T G885S (GGC→AGC)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,388,393 C→T R326R (CGG→CGA mscM ← miniconductance mechanosensitive channel MscM
RA 4,392,011 C→T T136T (ACC→ACT orn → oligoribonuclease
RA 4,393,834 C→T V78V (GTG→GTA queG ← epoxyqueuosine reductase
RA 4,399,496 C→T A82V (GCT→GTT)  miaA → tRNA dimethylallyltransferase
RA 4,399,890 C→T R213R (CGC→CGT miaA → tRNA dimethylallyltransferase
RA 4,417,079 A→G Y43C (TAT→TGT)  yjfP → carboxylesterase
RA 4,437,529 C→T intergenic (+34/‑178) cysQ → / → ytfI 3'(2'),5'‑bisphosphate nucleotidase/protein YtfI
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
RA 4,439,373 C→T intergenic (‑111/‑214) ytfJ ← / → ytfK PF09695 family protein YtfJ/stringent response modulator YtfK
RA 4,443,638 C→T A419A (GCC→GCT tamA → translocation and assembly module subunit TamA
RA 4,447,716 C→T S1202F (TCC→TTC)  tamB → translocation and assembly module subunit TamB
RA 4,451,585 C→T L176L (CTC→CTT ytfR → galactofuranose ABC transporter putative ATP binding subunit
RA 4,454,889 C→T G241S (GGT→AGT)  fbp ← fructose‑1,6‑bisphosphatase 1
RA 4,456,150 C→T T122T (ACC→ACT mpl → UDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
RA 4,460,211 C→T E52K (GAA→AAA)  nrdG ← anaerobic ribonucleoside‑triphosphate reductase activating protein
RA 4,468,302 C→T R226R (CGC→CGT mgtA → Mg(2(+)) importing P‑type ATPase
RA 4,470,103 C→T P827S (CCG→TCG)  mgtA → Mg(2(+)) importing P‑type ATPase
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,474,886 C→T L9F (CTT→TTT)  tabA → DUF386 domain‑containing toxin‑antitoxin biofilm protein TabA
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
JC JC 4,542,042 IS1 (+) +8 bp coding (6‑13/597 nt) fimE → regulator for fimA
RA 4,551,552 C→T intergenic (‑256/‑84) gntP ← / → uxuA fructuronate transporter/D‑mannonate dehydratase
RA 4,561,547 C→T A377T (GCG→ACG)  yjiJ ← putative transporter YjiJ
RA 4,571,194 C→T E127E (GAG→GAA yjiR ← fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
RA 4,573,105 C→T P231L (CCT→CTT)  yjiT → putative uncharacterized protein YjiT

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 361657 362203 547 108 [0] [0] 107 [lacA]–[lacY] [lacA],[lacY]
* * ÷ NC_000913 1978484 1979270 787 106 [0] [0] 127 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423806–3424234 3424452–3424239 6–647 33 [30] [30] 31 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 19796 =NA (NA)107 (1.050) 76/596 0.1 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 361656 0 (0.000)coding (205/612 nt) lacA galactoside O‑acetyltransferase
* ? NC_000913 = 258675NA (NA)106 (1.040) 79/598 0.1 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 362204 = 0 (0.000)coding (976/1254 nt) lacY lactose permease
* ? NC_000913 574591 =NA (NA)103 (1.010) 73/598 0.2 98.1% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 584978 = 2 (0.020)coding (656/954 nt) ompT omptin family outer membrane protease OmpT
* ? NC_000913 574591 =NA (NA)121 (1.180) 84/598 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 584981 0 (0.000)coding (653/954 nt) ompT omptin family outer membrane protease OmpT
* ? NC_000913 1207790 =22 (0.210)72 (0.740) 50/566 0.7 78.3% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 19 (0.200)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780525 (0.240)66 (0.680) 46/566 0.8 75.6% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 19 (0.200)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)112 (1.110) 81/590 0.1 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19784830 (0.000)106 (1.040) 73/598 0.2 100% intergenic (‑286/+35) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 1979271 =0 (0.000)111 (1.140)
+TTAAGTAATTGAGTG
79/568 0.1 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 2996360 = NA (NA)intergenic (+2504/+12) ygeI/insD4 protein YgeI/IS2 insertion element protein InsB
* ? NC_000913 4542682 =137 (1.340)82 (0.830) 57/580 0.5 39.9% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 114 (1.150)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 4542690143 (1.400)88 (0.890) 52/580 0.6 41.0% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 114 (1.150)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit