breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 41 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,037,4960AT45.0% 124.8 / inf 189intergenic (+161/‑23)alaT/rrlAtRNA‑Ala/23S ribosomal RNA
*NC_0009133,054,8940GT39.6% 109.5 / 49.6 106P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009132,767,4450CA38.7% 181.4 / 32.2 111intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009131,611,5070GT37.8% 97.3 / 10.6 82L116L (CTC→CTAdgcFputative diguanylate cyclase DgcF
*NC_0009133,054,8860GT37.1% 137.4 / 47.1 106Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009133,054,8880AC36.2% 173.0 / 13.8 106V186G (GTA→GGA) ygfBPF03695 family protein YgfB
*NC_0009132,477,2650AC34.8% 144.3 / 28.8 89L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009134,427,8220CA32.0% 259.2 / 21.8 147intergenic (‑400/+257)yjfZ/ytfBDUF2686 domain‑containing protein YjfZ/cell division protein YtfB
*NC_0009131,470,6270CA31.9% 231.3 / 15.0 119pseudogene (109/3495 nt)ydbAputative outer membrane protein, N‑terminal fragment
*NC_0009132,767,4490CA31.9% 239.6 / 11.5 117intergenic (+94/‑261)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009131,812,5090GT31.1% 234.4 / 30.3 135L208L (CTC→CTAydjOprotein YdjO
*NC_0009133,841,6630GA29.8% 103.4 / 16.7 67A26V (GCG→GTG) nepIpurine ribonucleoside exporter
*NC_0009133,970,2530GT29.8% 181.8 / 11.0 124E41* (GAG→TAG) wecBUDP‑N‑acetylglucosamine 2‑epimerase
*NC_0009133,741,8710GT29.5% 179.7 / 21.5 112S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR
*NC_000913847,9550GA29.2% 151.0 / 42.5 106A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009131,922,8150CA29.1% 161.6 / 23.3 110G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_000913834,6740CA28.7% 197.2 / 13.9 101L535L (CTC→CTAdinGATP‑dependent DNA helicase DinG
*NC_0009133,911,5330CA28.0% 287.9 / 14.8 161intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009134,296,2680TC27.8% 82.8 / 34.5 54intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009133,602,7060CA26.7% 112.4 / 29.3 75G14G (GGG→GGTftsEcell division protein FtsE

Marginal new junction evidence (lowest skew 10 of 15 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 696469127 (1.240)22 (0.220)
+TAGAAT
18/586 4.0 15.0% noncoding (36/75 nt) glnX tRNA‑Gln
?NC_000913 696802 = 127 (1.240)noncoding (29/75 nt) glnW tRNA‑Gln
* ? NC_000913 = 696741137 (1.340)13 (0.170) 8/442 5.3 11.2% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 106 (1.400)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 4606166104 (1.020)6 (0.080) 5/420 6.3 7.5% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 76 (1.060)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 128708449 (0.480)8 (0.080) 7/558 6.8 14.4% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 49 (0.510)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 781322107 (1.040)6 (0.070) 6/520 6.9 4.9% intergenic (+100/‑47) lysY/lysZ tRNA‑Lys/tRNA‑Lys
?NC_000913 781811 = 141 (1.580)intergenic (+159/‑274) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 3648146124 (1.210)5 (0.060) 5/482 7.0 5.1% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 88 (1.070)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 781016102 (1.000)6 (0.070) 6/524 7.0 4.9% intergenic (+98/‑52) lysW/valZ tRNA‑Lys/tRNA‑Val
?NC_000913 781809 = 144 (1.600)intergenic (+157/‑276) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 3270072 =54 (0.530)5 (0.050)
+25 bp
5/548 7.7 5.8% intergenic (+470/+144) yhaC/rnpB uncharacterized protein YhaC/RNase P catalytic RNA component
?NC_000913 4137788 = 122 (1.190)intergenic (‑131/+144) yijF/gldA DUF1287 domain‑containing protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
* ? NC_000913 = 281827547 (0.460)3 (0.040) 3/440 7.7 4.9% intergenic (‑1/+198) argY/argV tRNA‑Arg/tRNA‑Arg
?NC_000913 2818551 = 83 (1.100)intergenic (‑2/+2) argV/serV tRNA‑Arg/tRNA‑Ser
* ? NC_000913 = 252100671 (0.690)3 (0.040) 3/446 7.7 5.6% noncoding (76/76 nt) valU tRNA‑Val
?NC_000913 2521127 = 49 (0.640)intergenic (+1/‑46) valX/valY tRNA‑Val/tRNA‑Val